FastQCFastQC Report
Tue 31 May 2016
SRR212622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13469
Sequences flagged as poor quality0
Sequence length58
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2561.900660776598114No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2341.7373227411092138No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2321.722473828792041No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1831.3586754770213083No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT1641.2176108100081668No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1501.1136684237879575No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1411.04684831836068No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1290.9577548444576435No Hit
GTCCCGGGGGTTTGAATTTCTGATTCTCCAGCCTCCACTGGCCAAGTATTGGGTTACA1250.9280570198232979No Hit
GTCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1140.8463880020788477No Hit
GTCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1120.8315390897616749No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1080.8018412651273295No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG800.5939564926869108No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA630.4677407379909421No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCT630.4677407379909421No Hit
GTCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC610.45289182567376945No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA600.445467369515183No Hit
GTCCCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC580.43061845719801023No Hit
GTCCCGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA560.41576954488083745No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT540.4009206325636647No Hit
GTCCCGGGGAGCCTTGTAGAGACTGTGTGGTCCTGATGACTCTAGAGTATGGTGACTT540.4009206325636647No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT520.38607172024649195No Hit
GTCCCGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA510.37864726408790556No Hit
GTCCCGGGGAACGGAGTCCGTGCAGCACCGCTGTGGGAAAGTAAAAAGCAGAGCTTTG510.37864726408790556No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT490.3637983517707328No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT470.34894943945356005No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC470.34894943945356005No Hit
GTCCCGGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC410.3044027025020417No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA400.2969782463434554No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCT400.2969782463434554No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT390.28955379018486893No Hit
GTCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT360.2672804217091098No Hit
GTCCCGGGGAGTTGGCTCCCGACCTAGTCAAGAGCCACACCTTCAGAGGAAGGGGAGT360.2672804217091098No Hit
GTCCCGGGGAGCTTAGCATCCCTGGAGCCTACAGACCCCTCCCCATATATGCTAGACC360.2672804217091098No Hit
GTCCCGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGA360.2672804217091098No Hit
GTCCCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC350.2598559655505234No Hit
GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC350.2598559655505234No Hit
GTCCCGGGGATTGTTGAGCATCTTTGAGAAGAAAGTTCTGTAATGCCCTTCTCTCCAC340.25243150939193704No Hit
GTCCCGGGGGGGGCTGGAAAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA310.23015814091617787No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCCC300.2227336847575915No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT300.2227336847575915No Hit
GTCCCGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC300.2227336847575915No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA280.20788477244041872No Hit
GTCCCGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG270.20046031628183236No Hit
GTCCCGGGGAAGCTGGTTTATTAAGATTCTACCCAGCACTGGGTAAAGTTGTGCTGTC270.20046031628183236No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT260.19303586012324597No Hit
GTCCCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC260.19303586012324597No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.19303586012324597No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA250.1856114039646596No Hit
GTCCCGGGGAGTTGGGGTGGCTGGCTCTGGGGCAGGGATTGGGACTGTTTTTGGGAGT240.1781869478060732No Hit
GTCCCGGGGCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGAG230.17076249164748683No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC230.17076249164748683Illumina Single End Adapter 2 (96% over 25bp)
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT230.17076249164748683No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCA210.15591357933031405No Hit
GTCCCGGGGATTCCATTTGCAACTTATCCCCAACAGGTGGTGAAGCAGTATTTTCCAA210.15591357933031405No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCTCCCC200.1484891231717277No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT190.14106466701314127No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG190.14106466701314127No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA180.1336402108545549No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT180.1336402108545549No Hit
GTCCCGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA180.1336402108545549No Hit
GTCCCGGGGAGGTTGTTCGGCTGAAGCCTACCAGAAAGTTTGCTTACCTGGGGCGTCT180.1336402108545549No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG180.1336402108545549No Hit
GTCCCGGGGAGCCCAAGAGGCTGGGTCTCTAAAGATATTTCTAGTTTAGATAATAATT180.1336402108545549No Hit
GTCCCGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC170.12621575469596852No Hit
GTCCCGGGGATGTGTGGAAGTTTCTCACGTTGATGTTTTTGCCACAGCTTTTGCAGCG170.12621575469596852No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGGA160.11879129853738213No Hit
GTCCCGGGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTATGAGCCCACCAC160.11879129853738213No Hit
GTCCCGGGGCACAATGGCAAAGTTATTTGCACTTTTCACAGCATTGAACTGTGTATGG160.11879129853738213No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCAACTGCTCTTCCGAA160.11879129853738213No Hit
GTCCCGGGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGG160.11879129853738213No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCT150.11136684237879575No Hit
GTCCCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11136684237879575No Hit
GTCCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA140.10394238622020936No Hit
GTCCCGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC140.10394238622020936No Hit
GTCCCGGGGGTCAAAACCACTGTCTCCTGTGTGAGATACGTTTCGAACCGGAGCTCCG140.10394238622020936No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGGGCGGGGCCTTCT140.10394238622020936No Hit
GTCCCGGGGAATTTTAGGAACCCTGATGACATCCTGCTGTTCAGAGACCGTTTTGACG140.10394238622020936No Hit
GTCCCGGGGGCTCTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCTTCTCTCAGCT140.10394238622020936No Hit
GTCCCGGGGCAGTTGCGTTTCCATTCATTTTGGTACACTAGTCAAGACTACGAATGGA140.10394238622020936No Hit
GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC140.10394238622020936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTC503.6379788E-1252.00000449
CTCCTCT503.6379788E-1252.00000448
CGGGGCT1000.052.0000045
GGCCCTT251.5686726E-552.0000048
GGCCTGT503.6379788E-1252.0000049
CCTCTCC503.6379788E-1252.00000450
CCGGGGC2450.052.0000044
TCTCCCC503.6379788E-1252.00000452
CCCTTTT251.5686726E-552.00000410
GCCCTTT251.5686726E-552.0000049
AGCCTCC150.007066785452.030
TTTGAAT150.007066785452.011
AAGTATT150.007066785452.044
TGCACTC307.4158925E-752.016
ACTGCAC150.007066785452.014
AACGCAG550.052.023
GTTTGAA150.007066785452.010
GCCTCCA150.007066785452.031
CGGGGTG150.007066785452.05
AATTTCT150.007066785452.015
Produced by FastQC (version 0.11.5)