FastQCFastQC Report
Tue 31 May 2016
SRR212621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13680
Sequences flagged as poor quality0
Sequence length58
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2782.0321637426900585No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2661.9444444444444444No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2381.739766081871345No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2051.4985380116959064No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1711.25No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1381.0087719298245614No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1330.9722222222222222No Hit
GTCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1240.9064327485380117No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1200.8771929824561403No Hit
GTCCCGGGGGTTTGAATTTCTGATTCTCCAGCCTCCACTGGCCAAGTATTGGGTTACA1140.8333333333333334No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA880.6432748538011697No Hit
GTCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC840.6140350877192983No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG800.5847953216374269No Hit
GTCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC660.4824561403508772No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA590.43128654970760233No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT590.43128654970760233No Hit
GTCCCGGGGAGCCTTGTAGAGACTGTGTGGTCCTGATGACTCTAGAGTATGGTGACTT560.40935672514619886No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA550.402046783625731No Hit
GTCCCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC520.38011695906432746No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCT510.37280701754385964No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA490.358187134502924No Hit
GTCCCGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA460.3362573099415205No Hit
GTCCCGGGGATTGTTGAGCATCTTTGAGAAGAAAGTTCTGTAATGCCCTTCTCTCCAC450.3289473684210526No Hit
GTCCCGGGGAACGGAGTCCGTGCAGCACCGCTGTGGGAAAGTAAAAAGCAGAGCTTTG440.32163742690058483No Hit
GTCCCGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA420.30701754385964913No Hit
GTCCCGGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC410.29970760233918126No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT400.29239766081871343No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.27046783625730997No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT360.2631578947368421No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT360.2631578947368421No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCT360.2631578947368421No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC360.2631578947368421No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA340.24853801169590645No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT330.2412280701754386No Hit
GTCCCGGGGAGTTGGCTCCCGACCTAGTCAAGAGCCACACCTTCAGAGGAAGGGGAGT320.23391812865497078No Hit
GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC320.23391812865497078No Hit
GTCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT310.22660818713450293No Hit
GTCCCGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC310.22660818713450293No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT300.21929824561403508No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT300.21929824561403508No Hit
GTCCCGGGGAAGCTGGTTTATTAAGATTCTACCCAGCACTGGGTAAAGTTGTGCTGTC290.21198830409356723No Hit
GTCCCGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG290.21198830409356723No Hit
GTCCCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC290.21198830409356723No Hit
GTCCCGGGGGGGGCTGGAAAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA280.20467836257309943No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA270.19736842105263158No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCC260.19005847953216373No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCCC260.19005847953216373No Hit
GTCCCGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG250.18274853801169588No Hit
GTCCCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC250.18274853801169588No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT250.18274853801169588No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA250.18274853801169588No Hit
GTCCCGGGGAGCTTAGCATCCCTGGAGCCTACAGACCCCTCCCCATATATGCTAGACC240.17543859649122806No Hit
GTCCCGGGGATTCCATTTGCAACTTATCCCCAACAGGTGGTGAAGCAGTATTTTCCAA240.17543859649122806No Hit
GTCCCGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGA240.17543859649122806No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA220.16081871345029242No Hit
GTCCCGGGGCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGAG210.15350877192982457No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA210.15350877192982457No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG210.15350877192982457No Hit
GTCCCGGGGAGCCCAAGAGGCTGGGTCTCTAAAGATATTTCTAGTTTAGATAATAATT210.15350877192982457No Hit
GTCCCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.14619883040935672No Hit
GTCCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA190.1388888888888889No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCA190.1388888888888889No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG190.1388888888888889No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCTCCCC190.1388888888888889No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA180.13157894736842105No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCT180.13157894736842105No Hit
GTCCCGGGGGAATTGCTGAAACTGAGATTAAGGAGAATAGGTAACCACCTCCAAGTAA170.12426900584795322No Hit
GTCCCGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA160.11695906432748539No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCGCCGACTGCTCTTCCAAA160.11695906432748539No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAA160.11695906432748539No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA160.11695906432748539No Hit
GTCCCGGGGCACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTTAGGCT150.10964912280701754No Hit
GTCCCGGGGACTGCCCTGGAGGAGGATGGCACTGCCCAGAAGATGCAGCTGGGCTACC150.10964912280701754No Hit
GTCCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG150.10964912280701754No Hit
GTCCCGGGGAGTTGGGGTGGCTGGCTCTGGGGCAGGGATTGGGACTGTTTTTGGGAGT150.10964912280701754No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA150.10964912280701754No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT150.10964912280701754Illumina Single End Adapter 2 (96% over 32bp)
GTCCCGGGGTGGTCCATGTACTCGACGTTTAATGGAGTTTCCTTTAGGAAACTGCCTT140.10233918128654972No Hit
GTCCCGGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT140.10233918128654972No Hit
GTCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA140.10233918128654972No Hit
GTCCCGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA140.10233918128654972No Hit
GTCCCGGGGGGGCTGGTGTGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGAA140.10233918128654972No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10233918128654972No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC140.10233918128654972Illumina Single End Adapter 2 (96% over 25bp)
GTCCCGGGGCAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10233918128654972No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAAT140.10233918128654972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCT150.006999736752.12867744
CTGGGTA150.006999736752.12867739
TATGCTC150.006999736752.12867745
AGGACAC150.006999736752.12867746
GGGTAAA150.006999736752.12867741
CTCTCCC503.6379788E-1252.12867751
AGTATGC150.006999736752.12867743
ATGCTCA150.006999736752.12867746
CTCAGGC150.006999736752.12867749
AGTTGTG150.006999736752.12867747
AGCACCT150.006999736752.12867738
TCTCCCC503.6379788E-1252.12867752
ACTGGGT150.006999736752.12867738
TAAAGTT150.006999736752.12867744
TGTGCTG150.006999736752.12867750
TGAGTAT150.006999736752.12867741
GTAAAGT150.006999736752.12867743
TGGTTTA150.007102621751.9377314
CGCTGCT150.007102621751.9377321
TCCTTTC150.007102621751.9377315
Produced by FastQC (version 0.11.5)