##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212621_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13680
Sequences flagged as poor quality	0
Sequence length	58
%GC	58
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.00694444444444	38.0	36.0	39.0	33.0	40.0
2	36.51483918128655	38.0	35.0	39.0	32.0	40.0
3	36.63326023391813	38.0	36.0	39.0	32.0	40.0
4	36.23618421052632	38.0	36.0	39.0	30.0	40.0
5	35.75497076023392	38.0	35.0	39.0	28.0	40.0
6	35.59078947368421	38.0	35.0	39.0	30.0	39.0
7	36.71323099415205	38.0	36.0	39.0	32.0	40.0
8	36.75051169590643	38.0	37.0	39.0	33.0	40.0
9	36.748684210526314	38.0	37.0	39.0	33.0	40.0
10	35.693347953216374	38.0	35.0	39.0	29.0	39.0
11	35.89342105263158	38.0	35.0	39.0	31.0	39.0
12	35.64086257309941	38.0	35.0	39.0	30.0	39.0
13	35.226023391812866	38.0	34.0	39.0	29.0	39.0
14	35.39795321637427	38.0	34.0	39.0	29.0	39.0
15	35.5983918128655	38.0	35.0	39.0	30.0	39.0
16	35.11491228070175	38.0	34.0	39.0	28.0	39.0
17	34.50526315789474	36.0	33.0	38.0	27.0	39.0
18	34.731505847953215	37.0	33.0	38.0	28.0	39.0
19	34.04254385964912	36.0	33.0	38.0	26.0	39.0
20	34.244152046783626	36.0	33.0	38.0	27.0	39.0
21	33.622149122807016	36.0	32.0	38.0	25.0	39.0
22	33.52441520467836	36.0	32.0	38.0	25.0	39.0
23	33.34956140350877	36.0	31.0	38.0	25.0	39.0
24	33.57485380116959	36.0	32.0	38.0	25.0	39.0
25	33.3046783625731	36.0	31.0	38.0	25.0	39.0
26	32.89773391812866	36.0	31.0	38.0	23.0	39.0
27	32.710818713450294	35.0	31.0	38.0	23.0	39.0
28	31.863523391812866	35.0	29.0	38.0	20.0	39.0
29	32.169005847953215	35.0	30.0	38.0	22.0	39.0
30	31.626461988304094	35.0	29.0	38.0	19.0	39.0
31	30.99393274853801	35.0	28.0	38.0	17.0	39.0
32	30.706286549707603	34.0	28.0	38.0	16.0	39.0
33	31.126973684210526	35.0	29.0	38.0	18.0	39.0
34	29.72872807017544	33.0	27.0	38.0	12.0	38.0
35	28.537280701754387	33.0	23.0	37.0	9.0	38.0
36	30.524926900584795	35.0	29.0	38.0	5.0	39.0
37	30.00299707602339	35.0	28.0	38.0	2.0	39.0
38	29.32214912280702	34.0	27.0	38.0	2.0	39.0
39	29.818713450292396	35.0	28.0	38.0	2.0	39.0
40	29.38530701754386	34.0	27.0	38.0	2.0	39.0
41	29.55862573099415	35.0	27.0	38.0	2.0	39.0
42	29.86842105263158	35.0	28.0	38.0	2.0	39.0
43	29.199415204678363	35.0	26.0	38.0	2.0	39.0
44	29.56001461988304	35.0	28.0	38.0	2.0	39.0
45	28.926754385964912	34.0	27.0	38.0	2.0	39.0
46	28.409868421052632	34.0	25.0	38.0	2.0	39.0
47	27.847587719298247	33.0	23.0	38.0	2.0	39.0
48	28.36315789473684	34.0	25.0	38.0	2.0	39.0
49	28.10372807017544	33.0	24.0	38.0	2.0	39.0
50	27.58545321637427	33.0	23.0	38.0	2.0	39.0
51	28.05168128654971	35.0	25.0	38.0	2.0	39.0
52	27.4	33.0	23.0	38.0	2.0	39.0
53	27.321783625730994	33.0	23.0	37.0	2.0	39.0
54	27.316593567251463	33.0	23.0	37.0	2.0	39.0
55	26.944298245614036	33.0	22.0	38.0	2.0	39.0
56	26.98793859649123	34.0	20.0	38.0	2.0	39.0
57	26.590497076023393	34.0	17.0	38.0	2.0	39.0
58	25.468201754385966	33.0	2.0	36.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-3.575775633572647
1	2	-3.603029589376348
1	3	-5.639263628098771
1	4	-8.35609057630537
1	5	-10.802415292789714
1	6	-9.37604743943849
1	7	-11.061436667048127
1	8	-13.66606390903701
1	9	-11.58344081020746
1	10	-7.862697582989764
1	11	-6.577566492460079
1	12	-6.196439675914334
1	13	-6.931242342681966
1	14	-0.6524030494502568
1	15	-3.6916919837343833
1	16	-2.4772480821909895
1	17	-2.8621156553499674
1	18	-2.9440857698229976
1	19	-8.121385061983954
1	20	-0.7405795433639852
1	21	-2.181020407109809
1	22	-2.7754168150820604
1	23	-4.599443164656989
1	24	-3.71814562648251
1	25	2.103818194368692
1	26	-1.1963944397893655
1	27	2.1069914487387322
1	28	1.4888501308131659
1	29	-4.190289360514797
1	30	-2.962554036663949
1	31	-1.7394196968751956
1	32	-4.223163211451698
1	33	1.0033486093441617
1	34	2.8408133191985776
1	35	-4.749624326016544
1	36	-1.2168461288382062
1	37	4.947457336050608
1	38	3.326727482219937
1	39	-0.8707183406119121
1	40	-0.036399547412507616
1	41	3.3272728772632014
1	42	-2.3350627767055734
1	43	0.8868556361580779
1	44	4.977896915355302
1	45	-2.302286043777908
1	46	5.653533330060025
1	47	-0.13517114575453704
1	48	3.168440322935293
1	49	1.9461667666698546
1	50	2.6882826283193815
1	51	6.55567513966799
1	52	2.749533498443128
1	53	7.734410613287363
1	54	6.951937377530438
1	55	5.770586446899337
1	56	3.150949384293039
1	57	-1.2731340580286066
1	58	5.894932169009177
2	1	-4.575775633572647
2	2	-3.536362922709685
2	3	-2.5725969614321045
2	4	-0.15609057630536682
2	5	-0.469081959456382
2	6	0.7572858938948386
2	7	-1.6614366670481289
2	8	-1.3993972423703411
2	9	-1.6501074768741262
2	10	-0.7960309163230974
2	11	-0.9775664924600775
2	12	0.2035603240856645
2	13	0.8020909906513651
2	14	0.8142636172164046
2	15	-1.625025317067717
2	16	-0.47724808219098946
2	17	-3.462115655349969
2	18	-4.544085769822999
2	19	-7.054718395317288
2	20	-1.1405795433639838
2	21	-0.847687073776477
2	22	-1.7754168150820604
2	23	-1.7994431646569886
2	24	-3.518145626482511
2	25	-6.762848472297971
2	26	1.4036055602106359
2	27	-4.6930085512612685
2	28	0.2888501308131666
2	29	-0.9236226938481273
2	30	-5.162554036663948
2	31	1.0605803031248051
2	32	-0.623163211451697
2	33	-1.4633180573225069
2	34	4.307479985865239
2	35	4.317042340650122
2	36	6.183153871161792
2	37	6.080790669383941
2	38	-2.139939184446728
2	39	-0.3373850072785807
2	40	-1.236399547412507
2	41	-2.2727271227368
2	42	-1.801729443372242
2	43	-7.113144363841922
2	44	-1.2887697513113636
2	45	-3.168952710444575
2	46	-1.87980000327331
2	47	-5.7351711457545385
2	48	-4.431559677064708
2	49	-4.520499899996814
2	50	-1.1117173716806192
2	51	-6.777658193665346
2	52	-4.91713316822354
2	53	-5.132256053379301
2	54	-4.648062622469563
2	55	-2.3627468864339995
2	56	-2.2490506157069596
2	57	-0.47313405802860586
2	58	-0.10506783099082284
3	1	-1.825775633572647
3	2	-2.9363629227096837
3	3	-2.472596961432103
3	4	-7.60609057630537
3	5	-9.219081959456382
3	6	-3.0427141061051586
3	7	-6.5614366670481274
3	8	-6.7493972423703426
3	9	-5.750107476874128
3	10	-0.696030916323096
3	11	-4.577566492460079
3	12	-7.696439675914334
3	13	-2.3479090093486334
3	14	1.0142636172164075
3	15	-3.2750253170677155
3	16	-4.4772480821909895
3	17	-2.3621156553499674
3	18	-3.1940857698229976
3	19	-0.7047183953172862
3	20	0.25942045663601476
3	21	-2.597687073776477
3	22	-5.77541681508206
3	23	-2.3494431646569893
3	24	-3.46814562648251
3	25	-4.312848472297972
3	26	-3.9463944397893655
3	27	-2.8930085512612678
3	28	-6.011149869186834
3	29	-0.7736226938481288
3	30	-6.462554036663949
3	31	-1.2394196968751956
3	32	-4.473163211451698
3	33	-2.163318057322506
3	34	-5.992520014134758
3	35	-2.3329576593498764
3	36	2.783153871161794
3	37	5.2807906693839435
3	38	1.6600608155532726
3	39	2.71261499272142
3	40	0.9636004525874924
3	41	3.5772728772632014
3	42	1.4982705566277588
3	43	3.386855636158078
3	44	1.0612302486886378
3	45	6.531047289555424
3	46	-1.1798000032733107
3	47	3.864828854245463
3	48	4.418440322935293
3	49	0.029500100003186702
3	50	1.1882826283193815
3	51	-1.5276581936653457
3	52	1.0828668317764603
3	53	-1.1822560533793016
3	54	-0.7980626224695619
3	55	-3.562746886433999
3	56	-2.099050615706961
3	57	-0.7731340580286066
3	58	-6.605067830990823
4	1	-1.075775633572647
4	2	0.2303037439569806
4	3	-1.8059302947654388
4	4	-1.1894239096387054
4	5	-1.469081959456382
4	6	-1.2093807727718229
4	7	0.6052299996185369
4	8	6.027576296574466E-4
4	9	0.0832258564592081
4	10	1.303969083676904
4	11	-0.7442331591267433
4	12	0.6368936574190016
4	13	1.2354243239847023
4	14	-0.31906971611692825
4	15	-0.8583586504010512
4	16	-1.6439147488576538
4	17	-1.5287823220166317
4	18	-2.6107524364896655
4	19	1.3786149380160495
4	20	-3.2405795433639852
4	21	-3.181020407109809
4	22	0.8912498515846039
4	23	2.067223502009675
4	24	-0.051478959815845826
4	25	-1.8961818056313042
4	26	2.13693889354397
4	27	0.6069914487387322
4	28	2.8221834641465016
4	29	2.6430439728185355
4	30	4.370779296669387
4	31	-1.2394196968751956
4	32	2.6101701218816373
4	33	0.170015276010826
4	34	3.007479985865242
4	35	-0.24962432601654427
4	36	0.2831538711617938
4	37	3.2807906693839435
4	38	1.8267274822199404
4	39	1.1292816593880879
4	40	3.6302671192541567
4	41	2.9939395439298657
4	42	4.164937223294423
4	43	1.886855636158078
4	44	5.477896915355302
4	45	-0.6356193771112437
4	46	1.6535333300600215
4	47	-0.3018378124212049
4	48	0.6684403229352931
4	49	1.6128334333365189
4	50	-2.6450507050139507
4	51	-6.110991526998678
4	52	-5.083799834890208
4	53	-7.5989227200459695
4	54	-4.048062622469562
4	55	-6.062746886433999
4	56	-3.349050615706961
4	57	-3.9398007246952744
4	58	-6.438401164324155
5	1	-1.4091089669059826
5	2	-1.2696962560430194
5	3	-3.3059302947654388
5	4	-7.689423909638702
5	5	-7.802415292789714
5	6	-1.8760474394384943
5	7	-3.7281033337147917
5	8	-3.166063909037007
5	9	-1.916774143540792
5	10	-0.8626975829897603
5	11	-2.2442331591267433
5	12	1.303560324085666
5	13	0.23542432398470226
5	14	-9.15240304945026
5	15	-0.3583586504010512
5	16	-3.1439147488576573
5	17	-2.028782322016635
5	18	-1.110752436489662
5	19	-1.4547183953172862
5	20	-2.907246210030653
5	21	-3.847687073776477
5	22	-2.9420834817487282
5	23	-4.4327764979903215
5	24	-4.551478959815842
5	25	-7.22951513896464
5	26	-3.1963944397893655
5	27	-4.559675217927936
5	28	-1.177816535853502
5	29	-1.856956027181461
5	30	-0.629220703330617
5	31	-1.7394196968751956
5	32	-2.5564965447850305
5	33	-0.9966513906558383
5	34	-1.8258533474680902
5	35	-5.249624326016544
5	36	3.6164872044951295
5	37	3.447457336050608
5	38	3.9933941488866083
5	39	4.46261499272142
5	40	5.296933785920828
5	41	3.660606210596537
5	42	4.498270556627759
5	43	2.72018896949141
5	44	3.811230248688638
5	45	4.697713956222088
5	46	2.8201999967266893
5	47	-2.801837812421205
5	48	3.668440322935293
5	49	4.779500100003187
5	50	5.854949294986046
5	51	6.55567513966799
5	52	3.0828668317764603
5	53	4.734410613287363
5	54	-3.21472928913623
5	55	-2.896080219767331
5	56	-8.015717282373629
5	57	-7.439800724695274
5	58	-6.771734497657491
6	1	-2.1757756335726484
6	2	-1.3363629227096823
6	3	-1.3725969614321016
6	4	-3.156090576305367
6	5	-3.6690819594563813
6	6	-0.44271410610515716
6	7	-5.0614366670481274
6	8	-1.1993972423703454
6	9	-3.0501074768741248
6	10	-2.7960309163230974
6	11	-0.17756649246008038
6	12	-0.5964396759143327
6	13	-0.7979090093486363
6	14	-2.585736382783594
6	15	-0.8250253170677126
6	16	-3.6772480821909888
6	17	1.1378843446500326
6	18	-1.9440857698229976
6	19	-3.6547183953172855
6	20	-3.7405795433639852
6	21	-2.6476870737764777
6	22	-1.575416815082061
6	23	-2.5994431646569893
6	24	-3.1181456264825087
6	25	-4.1628484722979735
6	26	-0.796394439789367
6	27	-1.6930085512612685
6	28	-0.5111498691868341
6	29	-2.723622693848128
6	30	-2.3625540366639477
6	31	-0.339419696875197
6	32	-1.2231632114516984
6	33	-2.463318057322507
6	34	-1.2925200141347588
6	35	0.517042340650125
6	36	-4.816846128838208
6	37	-1.9192093306160558
6	38	2.260060815553274
6	39	-5.337385007278581
6	40	0.36360045258749096
6	41	2.5272728772632043
6	42	1.7982705566277595
6	43	0.08685563615807723
6	44	0.5112302486886371
6	45	1.2310472895554234
6	46	5.920199996726691
6	47	2.864828854245463
6	48	2.7684403229352945
6	49	6.4795001000031895
6	50	4.6882826283193815
6	51	1.8223418063346557
6	52	5.482866831776459
6	53	2.467743946620697
6	54	5.351937377530437
6	55	4.637253113566004
6	56	4.950949384293036
6	57	5.126865941971392
6	58	6.494932169009179
7	1	-3.075775633572647
7	2	-5.269696256043016
7	3	-5.639263628098771
7	4	-6.0227572429720375
7	5	-10.802415292789714
7	6	-1.8760474394384943
7	7	-4.89477000038146
7	8	-3.166063909037007
7	9	-3.0834408102074633
7	10	-2.5293642496564317
7	11	-4.244233159126747
7	12	-2.196439675914334
7	13	-5.5979090093486334
7	14	-3.6524030494502604
7	15	-5.358358650401048
7	16	-6.4772480821909895
7	17	-9.362115655349967
7	18	-4.77741910315633
7	19	-3.2880517286506183
7	20	-4.240579543363985
7	21	-4.514353740443145
7	22	-8.27541681508206
7	23	-10.266109831323657
7	24	-7.384812293149178
7	25	-8.896181805631304
7	26	-5.029727773122698
7	27	-8.059675217927936
7	28	-3.8444832025201663
7	29	-6.690289360514797
7	30	-4.795887369997281
7	31	-8.572753030208528
7	32	-6.723163211451698
7	33	-8.829984723989174
7	34	-10.659186680801426
7	35	-13.91629099268321
7	36	-6.550179462171538
7	37	-7.7192093306160565
7	38	-10.67327251778006
7	39	-9.204051673945248
7	40	-9.536399547412508
7	41	-4.172727122736799
7	42	-4.168396110038909
7	43	-4.77981103050859
7	44	-4.188769751311362
7	45	-3.802286043777908
7	46	-1.0131333366066428
7	47	-5.301837812421205
7	48	-3.164893010398039
7	49	-5.720499899996813
7	50	-1.1450507050139507
7	51	-3.7776581936653457
7	52	-3.0837998348902076
7	53	-0.5989227200459695
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119	23	1.2338901686763464
119	24	-3.5514789598158423
119	25	-0.06284847229797208
119	26	-4.529727773122698
119	27	-4.559675217927936
119	28	-6.844483202520166
119	29	-5.356956027181461
119	30	-6.629220703330617
119	31	0.09391363645813655
119	32	3.6101701218816373
119	33	-0.16331805732250615
119	34	-1.159186680801426
119	35	-3.5829576593498764
119	36	-8.383512795504874
119	37	-4.7192093306160565
119	38	-9.17327251778006
119	39	-10.53738500727858
119	40	-8.536399547412508
119	41	-6.506060456070131
119	42	-6.501729443372241
119	43	-9.113144363841922
119	44	-6.85543641797803
119	45	-9.802286043777908
119	46	-7.3464666699399785
119	47	-4.468504479087869
119	48	-5.498226343731375
119	49	-6.887166566663481
119	50	-7.3117173716806185
119	51	-4.777658193665346
119	52	-4.750466501556872
119	53	-6.265589386712634
119	54	-4.21472928913623
119	55	-3.896080219767331
119	56	-6.349050615706961
119	57	-2.6064673913619387
119	58	-7.105067830990823
120	1	-2.325775633572647
120	2	-3.9363629227096837
120	3	-3.972596961432103
120	4	-6.85609057630537
120	5	-7.219081959456382
120	6	-3.2927141061051586
120	7	-3.8114366670481274
120	8	-5.2493972423703426
120	9	-5.500107476874128
120	10	-1.446030916323096
120	11	0.42243350753992104
120	12	-0.1964396759143341
120	13	0.15209099065136655
120	14	0.014263617216407454
120	15	1.4749746829322845
120	16	1.2727519178090105
120	17	-1.3621156553499674
120	18	-1.9440857698229976
120	19	-0.2047183953172862
120	20	1.5094204566360148
120	21	2.652312926223523
120	22	2.7245831849179396
120	23	-1.5994431646569893
120	24	0.7818543735174899
120	25	2.437151527702028
120	26	2.3036055602106345
120	27	3.6069914487387322
120	28	1.7388501308131659
120	29	4.226377306151871
120	30	1.0374459633360509
120	31	2.5105803031248044
120	32	3.7768367885483016
120	33	1.8366819426774939
120	34	2.257479985865242
120	35	7.667042340650124
120	36	3.283153871161794
120	37	6.7807906693839435
120	38	5.160060815553273
120	39	5.21261499272142
120	40	3.2136004525874924
120	41	7.577272877263201
120	42	7.998270556627759
120	43	5.636855636158078
120	44	8.311230248688638
120	45	3.781047289555424
120	46	-0.4298000032733107
120	47	8.364828854245463
120	48	4.418440322935293
120	49	6.029500100003187
120	50	7.6882826283193815
120	51	1.4723418063346543
120	52	8.08286683177646
120	53	7.567743946620698
120	54	7.951937377530438
120	55	7.687253113566001
120	56	9.900949384293039
120	57	9.226865941971393
120	58	8.644932169009177
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33.0
3	2.0
4	11.0
5	7.0
6	15.0
7	30.0
8	20.0
9	28.0
10	33.0
11	58.0
12	69.0
13	65.0
14	76.0
15	79.0
16	106.0
17	120.0
18	153.0
19	138.0
20	170.0
21	177.0
22	240.0
23	267.0
24	238.0
25	232.0
26	277.0
27	344.0
28	418.0
29	499.0
30	525.0
31	670.0
32	771.0
33	936.0
34	1047.0
35	1284.0
36	1486.0
37	1499.0
38	1124.0
39	433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	56.812865497076025	24.605263157894736	2.7850877192982457	15.796783625730995
11	38.5891812865497	22.668128654970758	15.182748538011696	23.559941520467838
12	46.11842105263158	8.808479532163743	25.27046783625731	19.80263157894737
13	17.478070175438596	14.561403508771932	32.551169590643276	35.4093567251462
14	14.247076023391813	25.40204678362573	43.377192982456144	16.973684210526315
15	30.087719298245613	11.915204678362572	43.48684210526316	14.510233918128653
16	30.277777777777775	11.747076023391813	41.432748538011694	16.542397660818715
17	32.65350877192982	11.527777777777779	25.935672514619885	29.88304093567252
18	38.080269025513566	13.21002997295124	23.956429563564587	24.75327143797061
19	22.156432748538013	26.535087719298247	37.18567251461988	14.12280701754386
20	37.26608187134503	24.480994152046783	19.19590643274854	19.05701754385965
21	24.890350877192983	21.65204678362573	30.431286549707604	23.026315789473685
22	29.576023391812868	23.00438596491228	27.185672514619885	20.23391812865497
23	41.55701754385965	23.172514619883042	20.46783625730994	14.802631578947366
24	38.496966152496526	23.276555303750275	21.390452518458954	16.836026025294245
25	27.00292397660819	24.35672514619883	16.11842105263158	32.521929824561404
26	32.478070175438596	16.89327485380117	23.362573099415204	27.266081871345026
27	21.56030444964871	17.315573770491806	22.211651053864166	38.91247072599532
28	20.462022077637254	41.64778127056071	16.836026025294245	21.054170626507783
29	40.42397660818713	21.535087719298247	19.788011695906434	18.252923976608187
30	20.716636197440586	27.531992687385742	25.550274223034737	26.201096892138942
31	35.13157894736842	19.890350877192983	24.517543859649123	20.460526315789473
32	44.97733586781693	15.609007164790173	23.446410293902616	15.967246673490276
33	46.33326021788404	14.937486290853258	15.887987131680925	22.84126635958178
34	32.74362160976679	17.55976314058045	27.896776080122816	21.799839169529935
35	28.35209825997953	30.881707852025148	27.70141833601404	13.064775551981283
36	26.720292504570388	39.0127970749543	16.672760511883	17.59414990859232
37	38.42778793418647	26.793418647166362	18.544789762340034	16.23400365630713
38	26.992833113938865	33.26751499195554	20.045341524060262	19.694310370045343
39	34.059825934323115	25.583266291230895	18.869304468660864	21.487603305785125
40	29.49908592321755	24.0073126142596	21.184643510054848	25.30895795246801
41	29.32787244935274	27.111826226870473	23.952314780955167	19.60798654282162
42	29.46167349327092	22.030427150380337	22.118197776477473	26.38970157987127
43	21.74930525084101	25.771537223928625	21.566476524791575	30.91268100043879
44	29.059391456992394	18.96576945582212	23.720011702750146	28.254827384435345
45	30.19088714985738	23.425729539969282	19.48365391647773	26.899729393695605
46	29.827384435342307	30.56612053832651	20.74312463428906	18.863370392042132
47	22.093788865315677	23.286268198112516	29.504718706562294	25.11522423000951
48	28.595464520848573	18.858814923189467	27.85662033650329	24.689100219458666
49	32.59931231253201	19.343038993342603	27.880605750237763	20.17704294388763
50	18.703351382994292	17.70818088687253	30.13317722815747	33.4552905019757
51	24.250182882223847	17.944403803950255	28.73445501097293	29.07095830285296
52	21.35332845647403	19.53913679590344	36.664228237015365	22.44330651060717
53	15.398683247988295	18.3979517190929	41.08266276517922	25.120702267739574
54	17.35089645078668	21.32455177460666	35.1335528723015	26.190998902305157
55	18.42933469955354	17.192417477859916	34.46534436068213	29.91290346190441
56	17.612180660273772	23.526828197057316	23.658590147134177	35.20240099553473
57	26.00834492350487	21.696801112656466	20.825708220481662	31.469145743357004
58	22.132767327819657	30.000731903681476	27.966039669179537	19.90046109931933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	20.5
14	41.0
15	34.0
16	25.0
17	23.0
18	24.5
19	26.0
20	28.0
21	30.0
22	35.0
23	33.5
24	27.0
25	34.0
26	41.0
27	46.5
28	71.0
29	90.0
30	115.0
31	140.0
32	152.0
33	164.0
34	157.5
35	145.0
36	139.0
37	263.0
38	387.0
39	273.0
40	159.0
41	142.5
42	148.0
43	170.0
44	164.0
45	158.0
46	191.5
47	328.5
48	432.0
49	421.5
50	411.0
51	402.5
52	394.0
53	384.5
54	361.5
55	348.0
56	574.0
57	800.0
58	819.0
59	790.5
60	743.0
61	922.5
62	1102.0
63	1347.0
64	1592.0
65	1418.0
66	1191.0
67	1138.0
68	909.0
69	680.0
70	567.5
71	455.0
72	411.0
73	298.0
74	229.0
75	179.5
76	130.0
77	101.5
78	62.0
79	51.0
80	39.5
81	28.0
82	23.5
83	19.0
84	12.5
85	5.0
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.007309941520467837
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.007309941520467837
25	0.0
26	0.0
27	0.11695906432748539
28	0.007309941520467837
29	0.0
30	0.03654970760233918
31	0.0
32	0.014619883040935673
33	0.021929824561403508
34	0.007309941520467837
35	0.014619883040935673
36	0.03654970760233918
37	0.03654970760233918
38	0.043859649122807015
39	0.05116959064327486
40	0.03654970760233918
41	0.05116959064327486
42	0.058479532163742694
43	0.043859649122807015
44	0.058479532163742694
45	0.05116959064327486
46	0.058479532163742694
47	0.08040935672514621
48	0.07309941520467836
49	0.08040935672514621
50	0.10233918128654972
51	0.07309941520467836
52	0.07309941520467836
53	0.07309941520467836
54	0.10964912280701754
55	0.12426900584795322
56	0.1388888888888889
57	0.1388888888888889
58	0.12426900584795322
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	13680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.31871345029239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95781302733717	32.47076023391813
2	11.019237259534256	9.546783625730994
3	4.623692203847452	6.008771929824562
4	2.413094836314546	4.181286549707602
5	1.4174822814714816	3.070175438596491
6	1.0799865001687479	2.807017543859649
7	0.6749915626054674	2.046783625730994
8	0.5399932500843739	1.8713450292397662
9	0.5231184610192373	2.0394736842105265
>10	2.413094836314546	18.14327485380117
>50	0.16874789065136686	4.751461988304094
>100	0.16874789065136686	13.062865497076023
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	278	2.0321637426900585	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	266	1.9444444444444444	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	238	1.739766081871345	No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	205	1.4985380116959064	No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	171	1.25	No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC	138	1.0087719298245614	No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	133	0.9722222222222222	No Hit
GTCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	124	0.9064327485380117	No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	120	0.8771929824561403	No Hit
GTCCCGGGGGTTTGAATTTCTGATTCTCCAGCCTCCACTGGCCAAGTATTGGGTTACA	114	0.8333333333333334	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	88	0.6432748538011697	No Hit
GTCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	84	0.6140350877192983	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	80	0.5847953216374269	No Hit
GTCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	66	0.4824561403508772	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA	59	0.43128654970760233	No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	59	0.43128654970760233	No Hit
GTCCCGGGGAGCCTTGTAGAGACTGTGTGGTCCTGATGACTCTAGAGTATGGTGACTT	56	0.40935672514619886	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA	55	0.402046783625731	No Hit
GTCCCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	52	0.38011695906432746	No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCT	51	0.37280701754385964	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	49	0.358187134502924	No Hit
GTCCCGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	46	0.3362573099415205	No Hit
GTCCCGGGGATTGTTGAGCATCTTTGAGAAGAAAGTTCTGTAATGCCCTTCTCTCCAC	45	0.3289473684210526	No Hit
GTCCCGGGGAACGGAGTCCGTGCAGCACCGCTGTGGGAAAGTAAAAAGCAGAGCTTTG	44	0.32163742690058483	No Hit
GTCCCGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA	42	0.30701754385964913	No Hit
GTCCCGGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC	41	0.29970760233918126	No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT	40	0.29239766081871343	No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37	0.27046783625730997	No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	36	0.2631578947368421	No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT	36	0.2631578947368421	No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCT	36	0.2631578947368421	No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	36	0.2631578947368421	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA	34	0.24853801169590645	No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT	33	0.2412280701754386	No Hit
GTCCCGGGGAGTTGGCTCCCGACCTAGTCAAGAGCCACACCTTCAGAGGAAGGGGAGT	32	0.23391812865497078	No Hit
GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC	32	0.23391812865497078	No Hit
GTCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	31	0.22660818713450293	No Hit
GTCCCGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC	31	0.22660818713450293	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	30	0.21929824561403508	No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	30	0.21929824561403508	No Hit
GTCCCGGGGAAGCTGGTTTATTAAGATTCTACCCAGCACTGGGTAAAGTTGTGCTGTC	29	0.21198830409356723	No Hit
GTCCCGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	29	0.21198830409356723	No Hit
GTCCCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC	29	0.21198830409356723	No Hit
GTCCCGGGGGGGGCTGGAAAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA	28	0.20467836257309943	No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA	27	0.19736842105263158	No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCC	26	0.19005847953216373	No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCCC	26	0.19005847953216373	No Hit
GTCCCGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG	25	0.18274853801169588	No Hit
GTCCCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	25	0.18274853801169588	No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT	25	0.18274853801169588	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	25	0.18274853801169588	No Hit
GTCCCGGGGAGCTTAGCATCCCTGGAGCCTACAGACCCCTCCCCATATATGCTAGACC	24	0.17543859649122806	No Hit
GTCCCGGGGATTCCATTTGCAACTTATCCCCAACAGGTGGTGAAGCAGTATTTTCCAA	24	0.17543859649122806	No Hit
GTCCCGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGA	24	0.17543859649122806	No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA	22	0.16081871345029242	No Hit
GTCCCGGGGCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGAG	21	0.15350877192982457	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA	21	0.15350877192982457	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG	21	0.15350877192982457	No Hit
GTCCCGGGGAGCCCAAGAGGCTGGGTCTCTAAAGATATTTCTAGTTTAGATAATAATT	21	0.15350877192982457	No Hit
GTCCCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.14619883040935672	No Hit
GTCCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA	19	0.1388888888888889	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCA	19	0.1388888888888889	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG	19	0.1388888888888889	No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCTCCCC	19	0.1388888888888889	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA	18	0.13157894736842105	No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCT	18	0.13157894736842105	No Hit
GTCCCGGGGGAATTGCTGAAACTGAGATTAAGGAGAATAGGTAACCACCTCCAAGTAA	17	0.12426900584795322	No Hit
GTCCCGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA	16	0.11695906432748539	No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCGCCGACTGCTCTTCCAAA	16	0.11695906432748539	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAA	16	0.11695906432748539	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	16	0.11695906432748539	No Hit
GTCCCGGGGCACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTTAGGCT	15	0.10964912280701754	No Hit
GTCCCGGGGACTGCCCTGGAGGAGGATGGCACTGCCCAGAAGATGCAGCTGGGCTACC	15	0.10964912280701754	No Hit
GTCCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG	15	0.10964912280701754	No Hit
GTCCCGGGGAGTTGGGGTGGCTGGCTCTGGGGCAGGGATTGGGACTGTTTTTGGGAGT	15	0.10964912280701754	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA	15	0.10964912280701754	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	15	0.10964912280701754	Illumina Single End Adapter 2 (96% over 32bp)
GTCCCGGGGTGGTCCATGTACTCGACGTTTAATGGAGTTTCCTTTAGGAAACTGCCTT	14	0.10233918128654972	No Hit
GTCCCGGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	14	0.10233918128654972	No Hit
GTCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	14	0.10233918128654972	No Hit
GTCCCGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA	14	0.10233918128654972	No Hit
GTCCCGGGGGGGCTGGTGTGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGAA	14	0.10233918128654972	No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.10233918128654972	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	14	0.10233918128654972	Illumina Single End Adapter 2 (96% over 25bp)
GTCCCGGGGCAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.10233918128654972	No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAAT	14	0.10233918128654972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.021929824561403508	0.0	0.0	0.0	0.0
11	0.05116959064327485	0.0	0.0	0.0	0.0
12	0.05847953216374269	0.0	0.0	0.0	0.0
13	0.06578947368421052	0.0	0.0	0.0	0.0
14	0.11695906432748537	0.0	0.0	0.0	0.0
15	0.11695906432748537	0.0	0.0	0.0	0.0
16	0.11695906432748537	0.0	0.0	0.0	0.0
17	0.11695906432748537	0.0	0.0	0.0	0.0
18	0.11695906432748537	0.0	0.0	0.0	0.0
19	0.11695906432748537	0.0	0.0	0.0	0.0
20	0.16812865497076024	0.0	0.0	0.0	0.0
21	0.17543859649122806	0.0	0.0	0.0	0.0
22	0.17543859649122806	0.0	0.0	0.0	0.0
23	0.2046783625730994	0.0	0.0	0.0	0.0
24	0.21198830409356725	0.0	0.0	0.0	0.0
25	0.21929824561403508	0.0	0.0	0.0	0.0
26	0.21929824561403508	0.0	0.0	0.0	0.0
27	0.21929824561403508	0.0	0.0	0.0	0.0
28	0.29239766081871343	0.0	0.0	0.0	0.0
29	0.30701754385964913	0.0	0.0	0.0	0.0
30	0.3362573099415205	0.0	0.0	0.0	0.0
31	0.3362573099415205	0.0	0.0	0.0	0.0
32	0.3362573099415205	0.0	0.0	0.0	0.0
33	0.3362573099415205	0.0	0.0	0.0	0.0
34	0.3362573099415205	0.0	0.0	0.0	0.0
35	0.4605263157894737	0.0	0.0	0.0	0.0
36	0.4605263157894737	0.0	0.0	0.0	0.0
37	0.4605263157894737	0.0	0.0	0.0	0.0
38	0.4605263157894737	0.0	0.0	0.0	0.0
39	0.4605263157894737	0.0	0.0	0.0	0.0
40	0.47514619883040937	0.0	0.0	0.0	0.0
41	0.47514619883040937	0.0	0.0	0.0	0.0
42	0.47514619883040937	0.0	0.0	0.0	0.0
43	0.4824561403508772	0.0	0.0	0.0	0.0
44	0.4824561403508772	0.0	0.0	0.0	0.0
45	0.49707602339181284	0.0	0.0	0.0	0.0
46	0.49707602339181284	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCT	15	0.0069997367	52.128677	44
CTGGGTA	15	0.0069997367	52.128677	39
TATGCTC	15	0.0069997367	52.128677	45
AGGACAC	15	0.0069997367	52.128677	46
GGGTAAA	15	0.0069997367	52.128677	41
CTCTCCC	50	3.6379788E-12	52.128677	51
AGTATGC	15	0.0069997367	52.128677	43
ATGCTCA	15	0.0069997367	52.128677	46
CTCAGGC	15	0.0069997367	52.128677	49
AGTTGTG	15	0.0069997367	52.128677	47
AGCACCT	15	0.0069997367	52.128677	38
TCTCCCC	50	3.6379788E-12	52.128677	52
ACTGGGT	15	0.0069997367	52.128677	38
TAAAGTT	15	0.0069997367	52.128677	44
TGTGCTG	15	0.0069997367	52.128677	50
TGAGTAT	15	0.0069997367	52.128677	41
GTAAAGT	15	0.0069997367	52.128677	43
TGGTTTA	15	0.0071026217	51.93773	14
CGCTGCT	15	0.0071026217	51.93773	21
TCCTTTC	15	0.0071026217	51.93773	15
>>END_MODULE