FastQCFastQC Report
Tue 31 May 2016
SRR212620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14255
Sequences flagged as poor quality0
Sequence length58
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2601.823921431076815No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2241.5713784636969486No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2131.4942125569975448No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2081.459137144861452No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT1951.3679410733076114No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1551.0873377762188707No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1350.9470361276745002No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1330.9330059628200632No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1140.7997193967029113No Hit
GTCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1030.7225534900035075No Hit
GTCCCGGGGGTTTGAATTTCTGATTCTCCAGCCTCCACTGGCCAAGTATTGGGTTACA1000.701508242721852No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG810.5682216766047001No Hit
GTCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC780.5471764293230446No Hit
GTCCCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC630.4419501929147668No Hit
GTCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC600.4209049456331112No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA590.4138898632058926No Hit
GTCCCGGGGAGCCTTGTAGAGACTGTGTGGTCCTGATGACTCTAGAGTATGGTGACTT580.4068747807786741No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA550.3858295334970186No Hit
GTCCCGGGGATTGTTGAGCATCTTTGAGAAGAAAGTTCTGTAATGCCCTTCTCTCCAC530.37179936864258156No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT490.34373903893370744No Hit
GTCCCGGGGAGTTGGCTCCCGACCTAGTCAAGAGCCACACCTTCAGAGGAAGGGGAGT470.3297088740792704No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCT450.3156787092248334No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC440.3086636267976149No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA420.2946334619431778No Hit
GTCCCGGGGAACGGAGTCCGTGCAGCACCGCTGTGGGAAAGTAAAAAGCAGAGCTTTG390.2735882146615223No Hit
GTCCCGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA380.26657313223430373No Hit
GTCCCGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC330.23149772009821112No Hit
GTCCCGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA320.22448263767099264No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCA320.22448263767099264No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT300.2104524728165556No Hit
GTCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT300.2104524728165556No Hit
GTCCCGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGA300.2104524728165556No Hit
GTCCCGGGGAAGCTGGTTTATTAAGATTCTACCCAGCACTGGGTAAAGTTGTGCTGTC290.20343739038933706No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT290.20343739038933706No Hit
GTCCCGGGGAGCCCAAGAGGCTGGGTCTCTAAAGATATTTCTAGTTTAGATAATAATT270.18940722553490003No Hit
GTCCCGGGGCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGAG260.1823921431076815No Hit
GTCCCGGGGAGCTTAGCATCCCTGGAGCCTACAGACCCCTCCCCATATATGCTAGACC260.1823921431076815No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA250.175377060680463No Hit
GTCCCGGGGGGGGCTGGAAAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA250.175377060680463No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA240.16836197825324448No Hit
GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC240.16836197825324448No Hit
GTCCCGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG230.16134689582602596No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.16134689582602596No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA220.15433181339880744No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA220.15433181339880744No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA220.15433181339880744No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT220.15433181339880744No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA210.1473167309715889No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA210.1473167309715889No Hit
GTCCCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA210.1473167309715889No Hit
GTCCCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC210.1473167309715889No Hit
GTCCCGGGGATTCCATTTGCAACTTATCCCCAACAGGTGGTGAAGCAGTATTTTCCAA210.1473167309715889No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCT200.14030164854437038No Hit
GTCCCGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT200.14030164854437038No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT200.14030164854437038No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCCC200.14030164854437038No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT190.13328656611715187No Hit
GTCCCGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA190.13328656611715187No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG190.13328656611715187No Hit
GTCCCGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC190.13328656611715187No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCGCCGACTGCTCTTCCAAA190.13328656611715187No Hit
GTCCCGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC180.12627148368993335No Hit
GTCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12627148368993335No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG180.12627148368993335No Hit
GTCCCGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG180.12627148368993335No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCT180.12627148368993335No Hit
GTCCCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC180.12627148368993335No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCTCCCC180.12627148368993335No Hit
GTCCCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA170.11925640126271483No Hit
GTCCCGGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC170.11925640126271483No Hit
GTCCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA160.11224131883549632No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGGA160.11224131883549632No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11224131883549632No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG160.11224131883549632No Hit
GTCCCGGGGGGGCATGGTTAATATGCACCAAGAATGAAGTAATAAATGAGAGCTATTG150.1052262364082778No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT150.1052262364082778No Hit
GTCCCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTAC150.1052262364082778No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAAT150.1052262364082778No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA150.1052262364082778No Hit
GTCCCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1052262364082778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT353.3078322E-852.429085
GCCCTTT353.3078322E-852.429089
CGGGGCA457.2759576E-1152.4290775
GGCCTGT307.031813E-752.4290779
GCCTGTG307.031813E-752.42907710
GGGGAAA150.00684769852.4290776
GGAGTGC307.031813E-752.4290779
CCGGGGT353.4026016E-852.243824
AGCGGGT251.529965E-552.24381628
CAGCGGG251.529965E-552.24381627
GCGGGTA251.529965E-552.24381629
GCGCGCG150.00694474752.24381324
CGCGGCT150.00694474752.24381327
CGCGCGG150.00694474752.24381325
GGCGCGC203.3140104E-452.0598623
CGGGTAA251.5625337E-552.05985630
CTTTCAG150.007141913351.87719315
TGCAATA150.007141913351.87719341
TTCCGAA150.007141913351.87719352
GCGGCGC150.007141913351.87719321
Produced by FastQC (version 0.11.5)