FastQCFastQC Report
Tue 31 May 2016
SRR212619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21843
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10084.6147507210548No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5622.5729066520166644No Hit
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA3261.4924689831982787No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT2591.185734560271025No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2571.1765783088403607No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2110.9659845259350822No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC2010.9202032687817607No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1800.8240626287597858No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1800.8240626287597858No Hit
GTCCCGGGGATTCCATTTGCAACTTATCCCCAACAGGTGGTGAAGCAGTATTTTCC1800.8240626287597858No Hit
GTCCCGGGGGTTTGAATTTCTGATTCTCCAGCCTCCACTGGCCAAGTATTGGGTTA1170.5356407086938607No Hit
GTCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1060.48528132582520717No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCT970.44407819438721785No Hit
GTCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA830.37998443437256785No Hit
GTCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT810.3708281829419036No Hit
GTCCCGGGGAGCCTTGTAGAGACTGTGTGGTCCTGATGACTCTAGAGTATGGTGAC750.34335942864991076No Hit
GTCCCGGGGATTGTTGAGCATCTTTGAGAAGAAAGTTCTGTAATGCCCTTCTCTCC740.3387813029345786No Hit
GTCCCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT710.32504692578858213No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTT660.30215629721192144No Hit
GTCCCGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC650.2975781714965893No Hit
GTCCCGGGGAGCTTAGCATCCCTGGAGCCTACAGACCCCTCCCCATATATGCTAGA640.29300004578125716No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC620.28384379435059287No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT610.2792656686352607No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC570.2609531657739321No Hit
GTCCCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC560.2563750400586No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC550.25179691434326784No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG550.25179691434326784Illumina Single End Adapter 2 (96% over 30bp)
GTCCCGGGGAGTTGGCTCCCGACCTAGTCAAGAGCCACACCTTCAGAGGAAGGGGA520.23806253719727144No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG510.23348441148193932Illumina Single End Adapter 2 (95% over 23bp)
GTCCCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG440.2014375314746143No Hit
GTCCCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA430.19685940575928212No Hit
GTCCCGGGGGCTCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCG430.19685940575928212No Hit
GTCCCGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCA430.19685940575928212No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC420.19228128004395No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC410.18770315432861787No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCC400.18312502861328572No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.18312502861328572No Hit
GTCCCGGGGGGCCGCGCCGCTCGCCGCCGGGCTATCACTGACGTGTCTCTCGGTCG390.17854690289795358No Hit
GTCCCGGGGGGGGCTGGAAAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT390.17854690289795358No Hit
GTCCCGGGGAGGATTTGGGTTTGGAATACTATGTAATAGGAGAATCTTTTGAGACT380.17396877718262144No Hit
GTCCCGGGGAGACAGACAGATGAATAGGACTGGTGAGAAAGTTCCGTGGGTGAAGG370.1693906514672893No Hit
GTCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG350.160234400036625No Hit
GTCCCGGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGAT340.15565627432129286No Hit
GTCCCGGGGATGCTGCGCATCCTGGACCCTGCATATCCTGACGTAACACTGCCTTT330.15107814860596072No Hit
GTCCCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA320.14650002289062858No Hit
GTCCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG320.14650002289062858No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC320.14650002289062858No Hit
GTCCCGGGGAACGGAGTCCGTGCAGCACCGCTGTGGGAAAGTAAAAAGCAGAGCTT320.14650002289062858No Hit
GTCCCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC320.14650002289062858No Hit
GTCCCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA310.14192189717529644No Hit
GTCCCGGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCT310.14192189717529644No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC310.14192189717529644No Hit
GTCCCGGGGCTTTCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCAT310.14192189717529644No Hit
GTCCCGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA300.1373437714599643No Hit
GTCCCGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC290.13276564574463215No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC290.13276564574463215No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC290.13276564574463215No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA280.1281875200293No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTC280.1281875200293No Hit
GTCCCGGGGCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG270.12360939431396785No Hit
GTCCCGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC270.12360939431396785No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC270.12360939431396785No Hit
GTCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG270.12360939431396785No Hit
GTCCCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA260.11903126859863572No Hit
GTCCCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11903126859863572No Hit
GTCCCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCC260.11903126859863572No Hit
GTCCCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGC260.11903126859863572No Hit
GTCCCGGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCC250.11445314288330358No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTT250.11445314288330358No Hit
GTCCCGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT250.11445314288330358No Hit
GTCCCGGGGATTCCTGGGACTATCAAGCTCCTTAGGCTCAAATATGGGGATTTCAC250.11445314288330358No Hit
GTCCCGGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC250.11445314288330358No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTC250.11445314288330358No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTC240.10987501716797143No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCC230.10529689145263928Illumina Single End Adapter 2 (100% over 22bp)
GTCCCGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA230.10529689145263928No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCT230.10529689145263928No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAGCAGG230.10529689145263928No Hit
GTCCCGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC230.10529689145263928No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT230.10529689145263928Illumina Single End Adapter 2 (100% over 29bp)
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTT230.10529689145263928No Hit
GTCCCGGGGAAGCTGGTTTATTAAGATTCTACCCAGCACTGGGTAAAGTTGTGCTG230.10529689145263928No Hit
GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG230.10529689145263928No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT220.10071876573730715No Hit
GTCCCGGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGG220.10071876573730715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTC600.050.3101949
TAAACGC150.00811923550.3101947
TATATGG150.00811923550.3101950
CAGTATT309.587038E-750.3101946
ACGCACC150.00811923550.3101950
ATGGCTA150.00811923550.3101942
CATTTTA150.00811923550.3101945
CGGATGC150.00811923550.3101950
TTATATG150.00811923550.3101949
GCGGATG150.00811923550.3101949
AGTATTT309.587038E-750.3101947
CCTCTCC600.050.3101950
GCAGTAT309.587038E-750.3101945
GCTAAAC150.00811923550.3101945
TATTTTC309.587038E-750.3101949
CTAAACG150.00811923550.3101946
TTTATAT150.00811923550.3101948
AAACGCA150.00811923550.3101948
ATTTTAT150.00811923550.3101946
GGCGGAT150.00811923550.3101948
Produced by FastQC (version 0.11.5)