FastQCFastQC Report
Tue 31 May 2016
SRR212618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21861
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9054.139792324230364No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5352.4472805452632542No Hit
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA3431.569004162664105No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT3101.4180504094048763No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2591.1847582452769772No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC2491.1390146836832715No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2191.0017839989021546No Hit
GTCCCGGGGATTCCATTTGCAACTTATCCCCAACAGGTGGTGAAGCAGTATTTTCC2030.9285943003522255No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2010.9194455880334842No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1970.901148163396002No Hit
GTCCCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1220.5580714514432094No Hit
GTCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1170.5351996706463565No Hit
GTCCCGGGGGTTTGAATTTCTGATTCTCCAGCCTCCACTGGCCAAGTATTGGGTTA1000.4574356159370569No Hit
GTCCCGGGGAGCCTTGTAGAGACTGTGTGGTCCTGATGACTCTAGAGTATGGTGAC920.42084076666209236No Hit
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTT790.36137413659027495No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCT780.3567997804309044No Hit
GTCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA780.3567997804309044No Hit
GTCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT770.35222542427153375No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT750.34307671195279266No Hit
GTCCCGGGGGGCCGCGCCGCTCGCCGCCGGGCTATCACTGACGTGTCTCTCGGTCG680.3110562188371987No Hit
GTCCCGGGGGCTCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCG670.3064818626778281No Hit
GTCCCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC650.297333150359087No Hit
GTCCCGGGGATTGTTGAGCATCTTTGAGAAGAAAGTTCTGTAATGCCCTTCTCTCC630.2881844380403458No Hit
GTCCCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG600.27446136956223416No Hit
GTCCCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA570.26073830108412244No Hit
GTCCCGGGGAGTTGGCTCCCGACCTAGTCAAGAGCCACACCTTCAGAGGAAGGGGA570.26073830108412244No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC560.2561639449247518No Hit
GTCCCGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC540.24701523260601072No Hit
GTCCCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA520.23786652028726954No Hit
GTCCCGGGGAGCTTAGCATCCCTGGAGCCTACAGACCCCTCCCCATATATGCTAGA470.21499473949041673No Hit
GTCCCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC470.21499473949041673No Hit
GTCCCGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC460.21042038333104618No Hit
GTCCCGGGGAACGGAGTCCGTGCAGCACCGCTGTGGGAAAGTAAAAAGCAGAGCTT460.21042038333104618No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC450.20584602717167558No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC440.201271671012305No Hit
GTCCCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA430.19669731485293446No Hit
GTCCCGGGGAGACAGACAGATGAATAGGACTGGTGAGAAAGTTCCGTGGGTGAAGG420.19212295869356388No Hit
GTCCCGGGGATGCTGCGCATCCTGGACCCTGCATATCCTGACGTAACACTGCCTTT410.1875486025341933No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG390.1783998902154522Illumina Single End Adapter 2 (95% over 23bp)
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC390.1783998902154522No Hit
GTCCCGGGGGGGGCTGGAAAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT380.17382553405608162No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG360.16467682173734047Illumina Single End Adapter 2 (96% over 30bp)
GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG360.16467682173734047No Hit
GTCCCGGGGAGGATTTGGGTTTGGAATACTATGTAATAGGAGAATCTTTTGAGACT350.1601024655779699No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTC340.15552810941859935No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC340.15552810941859935No Hit
GTCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG340.15552810941859935No Hit
GTCCCGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCA340.15552810941859935No Hit
GTCCCGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC330.15095375325922875No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA320.1463793970998582No Hit
GTCCCGGGGCTCCTCACACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTCTGTCT320.1463793970998582No Hit
GTCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC320.1463793970998582No Hit
GTCCCGGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGAT320.1463793970998582No Hit
GTCCCGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT310.14180504094048763No Hit
GTCCCGGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCC310.14180504094048763No Hit
GTCCCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG310.14180504094048763No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT300.13723068478111708No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTC300.13723068478111708No Hit
GTCCCGGGGATTTACAGTTGGTGGTCTAACCGGAATTGTTTTATCCAACTCATCCC290.13265632862174648No Hit
GTCCCGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC290.13265632862174648No Hit
GTCCCGGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC290.13265632862174648No Hit
GTCCCGGGGTGCCCGCCAGGAGCTCAAGGCACGTGCCCGCTACCTGGCCGAAAAGT280.1280819724623759No Hit
GTCCCGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC280.1280819724623759No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1280819724623759No Hit
GTCCCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC280.1280819724623759No Hit
GTCCCGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC270.12350761630300536No Hit
GTCCCGGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCT270.12350761630300536No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC270.12350761630300536No Hit
GTCCCGGGGCTCCTCACACAGTGTGTGTGTGTGTGTGTGTGTGTGTCTCTGTCTGT270.12350761630300536No Hit
GTCCCGGGGGAGGCGTTACTCAGCCCAGGAAGTGTATTCTGTTGACATTGACTACT270.12350761630300536No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCA270.12350761630300536No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC260.11893326014363477No Hit
GTCCCGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA250.11435890398426422No Hit
GTCCCGGGGAAGCTGGTTTATTAAGATTCTACCCAGCACTGGGTAAAGTTGTGCTG250.11435890398426422No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC250.11435890398426422No Hit
GTCCCGGGGAGAACACAGGATGTCAGCGCCATCTTGTAACGGCGAATGTGAGGGCG250.11435890398426422No Hit
GTCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA230.10521019166552309No Hit
GTCCCGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA230.10521019166552309No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT230.10521019166552309Illumina Single End Adapter 2 (100% over 29bp)
GTCCCGGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTT220.1006358355061525No Hit
GTCCCGGGGAGCCCAAGAGGCTGGGTCTCTAAAGATATTTCTAGTTTAGATAATAA220.1006358355061525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTG150.00798858350.51627745
CGCCTGA150.00798858350.51627746
CTCCTCT451.10958354E-1050.51627748
CAGTATT150.00798858350.51627746
GTATTTT150.00798858350.51627748
TGATATG150.00798858350.51627750
AGTATTT150.00798858350.51627747
AGTATGG150.00798858350.51627746
CCTCTCC451.10958354E-1050.51627750
AGAGTAT150.00798858350.51627744
TATTTTC150.00798858350.51627749
TTGATAT150.00798858350.51627749
TCTTCCG150.00798858350.51627750
AGCGACT150.00798858350.51627750
ATTTTCC150.00798858350.51627750
TTTGTTA150.00798858350.51627748
AAGCGCC150.00806252850.3990743
TAGAGTA150.00806252850.3990743
CGTGTCT150.00806252850.3990742
AAAGCGC150.00806252850.3990742
Produced by FastQC (version 0.11.5)