FastQCFastQC Report
Tue 31 May 2016
SRR212617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219456
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA39571.8030949256342959No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT31931.454961358996792No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC29381.3387649460484106No Hit
TAGAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC26261.1965952172645087No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG20610.9391404199475065No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG18200.829323417906095No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT17680.8056284631087781No Hit
TAGAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC15980.7281641878098571No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA11140.5076188393117527No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA10890.49622703412073493No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.4456474190726159No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9270.4224081364829396No Hit
TAGAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA8170.3722841936424614No Hit
TAGAGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7630.34767789442986297No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.3262613006707495No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA6510.29664260717410323No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAA5750.26201151939340916No Hit
TAGAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5510.2510753864100321No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA5380.24515164771070283No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA5370.24469597550306213No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4760.21689997083697873No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA4720.21507728200641585No Hit
TAGAGAGGGACATCTCCAGGAGAATGCACATTATAGGTATGTAATGTGGAACCGACAC4650.2118875765529309No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.17543379994167396No Hit
TAGAGAGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3700.1685987168270633No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3570.16267497812773404No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC3470.15811825605132693No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA3440.15675123942840477No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.1540172061825605No Hit
TAGAGAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3370.1535615339749198No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3330.15173884514435695No Hit
TAGAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3310.15082750072907553No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA3300.15037182852143482No Hit
TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT3300.15037182852143482No Hit
TAGAGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA3130.14262540099154272No Hit
TAGAGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT3060.13943569553805774No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTCCG2970.13533464566929135No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC2940.13396762904636922No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA2830.12895523476232137No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC2830.12895523476232137No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2810.12804389034703995No Hit
TAGAGAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2740.12485418489355499No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA2730.12439851268591426No Hit
TAGAGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG2690.12257582385535142No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2670.12166447944007No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG2630.11984179060950714No Hit
TAGAGAGGGGGGCTGGACAGATGGTTCAGTGGTTAAGAGCACTGACTCCTCTTCTGTA2630.11984179060950714No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2580.1175634295713036No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA2560.11665208515602217No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2540.11574074074074073No Hit
TAGAGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG2530.11528506853310003No Hit
TAGAGAGGGATATTGGAAAATGACAATGTCTTTTTTCTTTCACTCTAAGAAACCATTC2530.11528506853310003No Hit
TAGAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG2430.11072834645669291No Hit
TAGAGAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCTGA2400.10936132983377078No Hit
TAGAGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2390.10890565762613008No Hit
TAGAGAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCTG2390.10890565762613008No Hit
TAGAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2380.10844998541848937No Hit
TAGAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC2340.10662729658792651No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA2340.10662729658792651No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGGTAAGAGCACCCGACTGCTCTTCTGA2330.1061716243802858No Hit
TAGAGAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA2310.10526027996500438No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT2280.10389326334208224No Hit
TAGAGAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2250.10252624671916011No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG2250.10252624671916011No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA2240.1020705745115194No Hit
TAGAGAGGGGCCCCCCAAATTTCCAGATAGTATCCCTAACTGCAACAGAAGAATTTTA2220.10115923009623798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCC203.427526E-452.04289649
TTCCCGA401.869921E-952.04289645
TAACCTG203.427526E-452.04289648
CTAACCT203.427526E-452.04289647
TCTAATC203.427526E-452.04289648
GTTATAA203.427526E-452.04289650
GGTAGGC203.427526E-452.04289648
CATGACT150.007188907452.04289243
AACCATT307.92008E-752.04289251
TATGGGT251.6427344E-552.04289252
ATTGTTA251.6427344E-552.04289251
TTACTGC150.007188907452.04289246
CATTCTA150.007188907452.04289245
ATACTAT150.007188907452.04289245
CTAAACG307.92008E-752.04289246
TACTGCA150.007188907452.04289247
TCTAAGA307.92008E-752.04289244
AGACCGT150.007188907452.04289252
GATGCTT150.007188907452.04289243
CCGACAC459.094947E-1152.04289252
Produced by FastQC (version 0.11.5)