FastQCFastQC Report
Tue 31 May 2016
SRR212616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230132
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA40671.7672466236768463No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT34291.4900144265030506No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC29651.2883910103766534No Hit
TAGAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC27001.1732397059079138No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG20670.8981801748561694No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT19310.8390836563363634No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG18940.8230059270331811No Hit
TAGAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC15890.6904732935880277No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA11710.5088384057845062No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA11320.4918916100325031No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.47537934750491023No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9880.42931882571741437No Hit
TAGAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA8280.3597935098117602No Hit
TAGAGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT8010.34806111275268103No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.3332869831227296No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA6670.28983366068169575No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA5990.2602854014217927No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAA5950.25854726852415133No Hit
TAGAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5620.24420767211861016No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA5100.2216119444492726No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA4560.1981471503311143No Hit
TAGAGAGGGACATCTCCAGGAGAATGCACATTATAGGTATGTAATGTGGAACCGACAC4130.17946222168146975No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4120.1790276884570594No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.17294422331531467No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.1603427598074149No Hit
TAGAGAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3630.15773556046095283No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3560.15469382789008046No Hit
TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT3440.14947942919715643No Hit
TAGAGAGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3410.14817582952392538No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA3380.14687222985069437No Hit
TAGAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3250.14122329793336No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA3250.14122329793336No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3240.14078876470894963No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC3150.13687796568925661No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTCCG3070.1334016998939739No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2930.12731823475222917No Hit
TAGAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC2900.12601463507899816No Hit
TAGAGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA2900.12601463507899816No Hit
TAGAGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT2870.12471103540576713No Hit
TAGAGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG2770.12036570316166374No Hit
TAGAGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG2730.11862757026402239No Hit
TAGAGAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCTGA2720.11819303703961205No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA2690.11688943736638104No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA2670.11602037091756034No Hit
TAGAGAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2640.11471677124432933No Hit
TAGAGAGGGATATTGGAAAATGACAATGTCTTTTTTCTTTCACTCTAAGAAACCATTC2620.11384770479550867No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2610.11341317157109833No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA2580.11210957189786731No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2510.10906783932699494No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGGTAAGAGCACCCGACTGCTCTTCTGA2510.10906783932699494No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2480.10776423965376393No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.10646063998053291No Hit
TAGAGAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2440.10602610675612258No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA2440.10602610675612258No Hit
TAGAGAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCTG2430.10559157353171225No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT2420.10515704030730189No Hit
TAGAGAGGGGGGCTGGACAGATGGTTCAGTGGTTAAGAGCACTGACTCCTCTTCTGTA2420.10515704030730189No Hit
TAGAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2380.10341890740966055No Hit
TAGAGAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2360.10254984096083987No Hit
TAGAGAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG2350.10211530773642952No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC2340.10168077451201919No Hit
TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG2340.10168077451201919No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA2330.10124624128760885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT150.007166757752.08431624
TCCATGT150.007166757752.08431626
AGTCCCC150.007166757752.08431624
CGACGTA150.007166757752.08431627
CAATTTG150.007166757752.08431627
CAACATC150.007166757752.08431624
CGCGATC150.007166757752.08431625
TCTAGGT150.007166757752.08431624
AACGACG150.007166757752.08431625
ATGGTGG150.007166757752.08431625
CATAAGT353.816058E-852.08431626
TCATAAG307.8783705E-752.08431625
CGCACAG150.007166757752.08431624
CTAGGTA150.007166757752.08431625
ATGCACA307.8783705E-752.08431624
ACGACGT150.007166757752.08431626
TGCAATT150.007166757752.08431625
GATCGAA203.4144343E-452.08431226
AGATAGT203.4144343E-452.08431225
CGCGGAC203.4144343E-452.08431225
Produced by FastQC (version 0.11.5)