FastQCFastQC Report
Tue 31 May 2016
SRR212615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239007
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA39021.6325881668737736No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT32621.364813582865774No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC29411.2305078930742614No Hit
TAGAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC24551.0271665683431865No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG21070.8815641382888367No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT18240.7631575644227994No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG17600.7363801060219993No Hit
TAGAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC16280.6811515980703494No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA11240.4702791131640496No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA11190.4681871242264871No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9710.40626425167463714No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.40291706937453714No Hit
TAGAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA8200.34308618576024974No Hit
TAGAGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7320.30626718045914975No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.29036806453367475No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA6780.28367369993347474No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA5710.2389051366696373No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAA5660.2368131477320748No Hit
TAGAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4940.20668850703117483No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA4870.20375972251858734No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA4670.19539176676833733No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC3790.15857276146723737No Hit
TAGAGAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3770.15773596589221237No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.15187839686703736No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.15104160129201236No Hit
TAGAGAGGGACATCTCCAGGAGAATGCACATTATAGGTATGTAATGTGGAACCGACAC3540.14811281677942487No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3470.14518403226683738No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA3330.13932646324166237No Hit
TAGAGAGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3330.13932646324166237No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3270.1368160765165874No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTCCG3210.1343056897915124No Hit
TAGAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3180.1330504964289749No Hit
TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT3070.12844812076633738No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC3060.1280297229788249No Hit
TAGAGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA3050.1276113251913124No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA2930.1225905517411624No Hit
TAGAGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT2890.1209169605911124No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA2860.11966176722857488No Hit
TAGAGAGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2740.1146409937784249No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAGCAGTGG2630.1100386181157874No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2620.1096202203282749No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGGTAAGAGCACCCGACTGCTCTTCTGA2580.10794662917822491No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2560.10710983360319991No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA2560.10710983360319991No Hit
TAGAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC2550.10669143581568741No Hit
TAGAGAGGGATATTGGAAAATGACAATGTCTTTTTTCTTTCACTCTAAGAAACCATTC2510.10501784466563742No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2510.10501784466563742No Hit
TAGAGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG2500.1045994468781249No Hit
TAGAGAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2490.10418104909061242No Hit
TAGAGAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCTGA2470.10334425351558742No Hit
TAGAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2460.1029258557280749No Hit
TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG2440.1020890601530499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA203.3321147E-452.3431249
ACTGCAT203.3321147E-452.34312410
GGGTACT203.3321147E-452.3431247
GGATCAT203.3321147E-452.3431248
GGCATTT203.3321147E-452.3431248
GGATTCA353.6705387E-852.3431248
GGACAGC203.3321147E-452.3431248
AGGGCGA203.3321147E-452.3431246
GTGAATA353.6705387E-852.3431249
ATATTGG458.731149E-1152.34312410
AACCGAG150.00702792952.3431210
GACAATA251.5881413E-552.343129
CTATAAC150.00702792952.3431210
GGATCTA150.00702792952.343128
GATATTG503.6379788E-1252.343129
ACTAATC307.614508E-752.3431210
GAGTTAT150.00702792952.343129
GATTCAT150.00702792952.343129
GACTAAT307.614508E-752.343129
TTCACTC459.094947E-1152.07904439
Produced by FastQC (version 0.11.5)