FastQCFastQC Report
Tue 31 May 2016
SRR212614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285183
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT64772.2711732466521495No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC63562.2287443501190465No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC43381.5211285385173732No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC37511.3152957925262025No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC30501.0694887142641742No Hit
TAGAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA23550.825785548226928No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA18600.6522127896824145No Hit
TAGAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16920.5933032473885189No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.5147571909966583No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14130.4954713289361568No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.3986913665961856No Hit
TAGAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10390.3643274669247466No Hit
TAGAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC9950.3488987772763454No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC9610.33697660800258084No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC8800.308573792968024No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC7340.25737859549832914No Hit
TAGAGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA6820.23914468955021864No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC6720.235638169175582No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAGCAGT6720.235638169175582No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.2058327459911706No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.19846905320443364No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC5530.19391057671740602No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC5530.19391057671740602No Hit
TAGAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT5170.18128710336871412No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTC4830.16936493409494957No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCT4630.1623518933456763No Hit
TAGAGAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4430.155338852596403No Hit
TAGAGAGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4380.15358559240908468No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4380.15358559240908468No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4380.15358559240908468No Hit
TAGAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4210.1476245077722024No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT3820.13394907831111952No Hit
TAGAGAGGGACATCTCCAGGAGAATGCACATTATAGGTATGTAATGTGGAACCGAC3650.12798799367423724No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3600.12623473348691894No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC3510.12307886514974595No Hit
TAGAGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCC3490.12237756107481862No Hit
TAGAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3490.12237756107481862No Hit
TAGAGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC3440.1206243008875003No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC3430.12027364885003665No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCA3160.11080604383851772No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTG3120.10940343568866308No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTT3110.1090527836511994No Hit
TAGAGAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3110.1090527836511994No Hit
TAGAGAGGGTGAATATAAGCACATTAATACTGAGAATATTTTTGTTAGCTTCCTTC3080.10800082753880842No Hit
TAGAGAGGGATATTGGAAAATGACAATGTCTTTTTTCTTTCACTCTAAGAAACCAT3080.10800082753880842No Hit
TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG3040.10659821938895375No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.10659821938895375No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGGTAAGAGCACCCGACTGCTCTTCT2860.10028648271460783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAC252.0055642E-550.32306350
CATAGTA252.0055642E-550.32306350
TAATCGC252.0055642E-550.32306350
TGCGCCG150.00821271750.3230650
CATCGAA700.050.3230650
TTCAATA150.0082184750.31414849
CACGAGC150.0082184750.31414849
TAACCTG150.0082184750.31414848
AATCTTT150.0082184750.31414849
GGCGGAT204.0513446E-450.31414848
CGAACCG150.00822998350.29633347
CTAACCT150.00822998350.29633347
GCGAACC150.00823574450.28743446
GTATCTG204.0619785E-450.28743446
AATGTCC204.0619785E-450.28743446
TCTGTAA204.0690802E-450.2696445
TCGCACC150.00824727650.26963845
CGTTGAG150.00824727650.26963845
TCACTAA150.00824727650.26963845
ATTATCC301.0070034E-650.26963845
Produced by FastQC (version 0.11.5)