FastQCFastQC Report
Tue 31 May 2016
SRR212613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255951
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC57992.265668038022903No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT55052.1508023020031177No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC38871.51865005411192No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC37011.4459798945891988No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC29071.1357642673793031No Hit
TAGAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA22030.8607116205836273No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA17060.6665338287406574No Hit
TAGAGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16610.6489523385335474No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14360.5610448874979977No Hit
TAGAGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC13430.5247098077366371No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.48368633058671384No Hit
TAGAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9740.3805415880383355No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.37272759239073106No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC8730.3410809100179331No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC7890.30826212829799454No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC6900.26958284984235265No Hit
TAGAGAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA6360.2484850615938207No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC5740.22426167508624698No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.20121038792581392No Hit
TAGAGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC5060.19769408988439194No Hit
TAGAGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4890.1910521935839282No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.1894893944544073No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC4630.1808939992420424No Hit
TAGAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4620.1805032994596622No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCT4550.17776840098300065No Hit
TAGAGAGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4240.16565670772921381No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4210.16448460838207313No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTC4180.16331250903493247No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAGCAGT4170.16292180925255223No Hit
TAGAGAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3880.15159151556352582No Hit
TAGAGAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3690.14416821969830163No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3510.13713562361545764No Hit
TAGAGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC3470.13557282448593677No Hit
TAGAGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3270.12775882883833234No Hit
TAGAGAGGGACATCTCCAGGAGAATGCACATTATAGGTATGTAATGTGGAACCGAC3240.12658672949119168No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3230.12619602970881144No Hit
TAGAGAGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3220.12580532992643123No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT3210.12541463014405102No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC3180.12424253079691035No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC3160.1234611312321499No Hit
TAGAGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3030.11838203406120704No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCA2950.11525643580216526No Hit
TAGAGAGGGATATTGGAAAATGACAATGTCTTTTTTCTTTCACTCTAAGAAACCAT2910.11369363667264437No Hit
TAGAGAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTT2900.11330293689026415No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA2890.11291223710788392No Hit
TAGAGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2760.10783313993694105No Hit
TAGAGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2760.10783313993694105No Hit
TAGAGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCC2750.10744244015456084No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.1066610405898004No Hit
TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA2720.10627034080742018No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTC2660.10392614211313886No Hit
TAGAGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.10158194341885751No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTG2560.10001914428933664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT251.9955225E-550.36327750
ACGCACC309.937485E-750.36327750
CAATTAG150.00818585950.36327750
CGTAGTA150.00818585950.36327750
GCCGTGT150.00818585950.36327750
CGATGGC150.00819864650.343449
AGCAATT150.00820504450.33346648
TCGATGG150.00820504450.33346648
CTCGCTT150.00820504450.33346648
AATCCCC150.00821144750.32353647
GTCGATG150.00821144750.32353647
CTGCGTA150.00821144750.32353647
ATACTCT252.004867E-550.32353647
CGGGTAT150.008217853550.3136146
TGACATG150.008217853550.3136146
CGAATCC150.00823709650.28385545
ACCCGCG150.00823709650.28385545
ATTGTAT252.0142475E-550.2838545
ATGGAGC150.00824351650.27394544
ATGGCTA301.0073654E-650.26403842
Produced by FastQC (version 0.11.5)