FastQCFastQC Report
Tue 31 May 2016
SRR212612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83530
Sequences flagged as poor quality0
Sequence length58
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40274.821022387166288No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT22792.7283610678798036No Hit
TAGTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC12391.4832994133844126No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10851.2989345145456723No Hit
TAGTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10071.2055548904585178No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7720.924218843529271No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6320.7566143900395067No Hit
TAGTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT6030.7218963246737699No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5460.6536573686100803No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.4237998323955465No Hit
TAGTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3070.3675326230096971No Hit
TAGTCGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3070.3675326230096971No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.241829282892374No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.22866036154674968No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.22746318687896566No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1610.19274512151322878Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1370.16401292948641208No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.15443553214413983No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.14725248413743564No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.14366096013408355No Hit
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1190.14246378546629954No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1110.1328863881240273Illumina Single End Adapter 2 (96% over 32bp)
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1070.12809768945289118No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.12690051478510717No Hit
TAGTCGGGGTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGGTTC990.11852029211061893No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA980.1173231174428349No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.11014006943613074No Hit
TAGTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT870.10415419609721059No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC860.10295702142942656Illumina Single End Adapter 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC150.00718044852.02995751
ATTAAGC251.6363063E-552.02995751
TTAAGCC251.6363063E-552.02995752
CGTTCCT150.007197489551.99880618
ACAAATT150.007197489551.99880626
GGATGTG150.007197489551.9988068
TGGTTAG150.007197489551.99880637
GGGTTTA150.007197489551.9988068
AAACTCT150.007197489551.99880634
CCAACGC150.007197489551.99880621
GGGTCAG150.007197489551.9988067
GGCACCC307.9067104E-751.99880637
TTCGCTA150.007197489551.99880632
TTCCGTT150.007197489551.99880615
TTTCCGT150.007197489551.99880614
GTTCCTA150.007197489551.99880619
AAGAGTG150.007197489551.99880610
GGATTTC150.007197489551.9988068
GGATCGC150.007197489551.99880635
GTCGAGT150.007197489551.99880631
Produced by FastQC (version 0.11.5)