##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212612_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 83530 Sequences flagged as poor quality 0 Sequence length 58 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.53250329223034 38.0 35.0 39.0 32.0 40.0 2 34.76135520172393 38.0 33.0 39.0 26.0 40.0 3 34.48297617622411 38.0 33.0 39.0 25.0 40.0 4 34.11047527834311 38.0 33.0 39.0 23.0 40.0 5 35.34194900035915 38.0 35.0 39.0 26.0 40.0 6 35.077014246378546 38.0 33.0 39.0 28.0 40.0 7 35.78305997845086 38.0 35.0 39.0 30.0 40.0 8 35.613264695319046 38.0 35.0 39.0 29.0 40.0 9 35.41173231174428 38.0 35.0 39.0 28.0 40.0 10 33.047707410511194 36.0 31.0 39.0 22.0 39.0 11 34.215275948760926 37.0 33.0 39.0 26.0 40.0 12 34.15660241829283 37.0 32.0 39.0 26.0 40.0 13 33.93304202083084 37.0 32.0 39.0 25.0 40.0 14 34.37483538848318 38.0 33.0 39.0 26.0 40.0 15 34.201400694361304 37.0 33.0 39.0 26.0 40.0 16 33.53032443433497 36.0 32.0 39.0 23.0 40.0 17 33.72187238118041 36.0 33.0 39.0 24.0 40.0 18 33.602466179815636 36.0 32.0 39.0 23.0 40.0 19 33.21048725008979 36.0 31.0 39.0 23.0 40.0 20 33.4390039506764 36.0 32.0 39.0 23.0 40.0 21 33.40306476714953 36.0 32.0 39.0 23.0 40.0 22 33.245253202442235 36.0 32.0 39.0 23.0 40.0 23 32.66079252963007 36.0 31.0 38.0 22.0 40.0 24 32.86186998683108 36.0 31.0 38.0 23.0 40.0 25 32.689608523883635 36.0 31.0 38.0 22.0 40.0 26 32.45697354243984 36.0 31.0 38.0 19.0 40.0 27 32.5171794564827 36.0 31.0 38.0 20.0 39.0 28 31.886783191667664 35.0 30.0 38.0 18.0 39.0 29 31.98786064886867 36.0 30.0 38.0 18.0 39.0 30 31.349551059499582 35.0 30.0 38.0 14.0 39.0 31 30.586208547827127 35.0 29.0 38.0 2.0 39.0 32 30.291033161738298 35.0 28.0 38.0 2.0 39.0 33 30.276427630791332 35.0 28.0 38.0 2.0 39.0 34 30.00120914641446 35.0 28.0 38.0 2.0 39.0 35 29.464683347300372 35.0 27.0 38.0 2.0 39.0 36 29.24927570932599 35.0 26.0 39.0 2.0 39.0 37 28.969974859331977 35.0 26.0 39.0 2.0 39.0 38 28.913013288638812 35.0 25.0 39.0 2.0 39.0 39 28.691344427151922 35.0 25.0 39.0 2.0 39.0 40 28.43465820663235 35.0 24.0 39.0 2.0 39.0 41 28.51828085717706 35.0 24.0 39.0 2.0 39.0 42 28.515048485574045 35.0 25.0 39.0 2.0 39.0 43 28.376463546031367 35.0 24.0 39.0 2.0 39.0 44 28.191500059858733 35.0 23.0 39.0 2.0 39.0 45 28.0535376511433 35.0 23.0 39.0 2.0 39.0 46 28.106368969232612 35.0 23.0 39.0 2.0 39.0 47 28.050365138273673 35.0 23.0 39.0 2.0 39.0 48 27.886256434813838 35.0 23.0 39.0 2.0 39.0 49 27.69971267807973 35.0 22.0 39.0 2.0 39.0 50 27.370705135879327 35.0 19.0 39.0 2.0 39.0 51 27.062552376391714 35.0 9.0 39.0 2.0 39.0 52 26.91752663713636 35.0 2.0 39.0 2.0 39.0 53 26.568107266850234 35.0 2.0 39.0 2.0 39.0 54 26.341709565425596 35.0 2.0 39.0 2.0 39.0 55 25.95832634981444 35.0 2.0 38.0 2.0 39.0 56 25.320986471926254 35.0 2.0 38.0 2.0 39.0 57 25.098527475158626 34.0 2.0 38.0 2.0 39.0 58 24.822518855501016 34.0 2.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -5.715320196945534 1 2 -16.112224525703994 1 3 -14.659096014425277 1 4 -21.68572657320732 1 5 -16.038340450634465 1 6 -13.605229176805466 1 7 -5.339192752933073 1 8 -7.153434255347058 1 9 -6.9465205708631 1 10 -19.195108290510248 1 11 1.585253394698988 1 12 1.8591726153032724 1 13 1.9083104532515236 1 14 1.2685990222283436 1 15 1.6844339729200755 1 16 2.6667512007430574 1 17 2.5597304108725254 1 18 2.7761803163256715 1 19 2.9261107828927564 1 20 2.798334287145579 1 21 2.971004391893956 1 22 3.180163142520186 1 23 3.7602211151818246 1 24 3.881694449565721 1 25 3.6792111566357946 1 26 4.097932012930304 1 27 4.2807497996528845 1 28 4.614615316287331 1 29 4.576592321570008 1 30 5.295792714073798 1 31 5.941507005683071 1 32 6.28693231883593 1 33 6.6617066823631035 1 34 7.1948534255182395 1 35 1.4560733740364533 1 36 -21.788955919368966 1 37 -21.523152054500667 1 38 -20.428804178991744 1 39 -22.214192283515466 1 40 -20.55802609660209 1 41 -17.599706489791224 1 42 -17.772897328642824 1 43 -10.605861645381456 1 44 -5.60041378305505 1 45 -0.38207224053014954 1 46 9.113049901874355 1 47 9.163302837839375 1 48 9.262727035713734 1 49 9.550631672653864 1 50 9.940092596443353 1 51 10.189638757871819 1 52 10.483398918286639 1 53 10.858952180368508 1 54 10.86411783565471 1 55 11.334947932654767 1 56 12.086158318062619 1 57 12.234432964084426 1 58 12.73253815394865 2 1 3.284679803054466 2 2 5.3877754742960065 2 3 5.840903985574723 2 4 8.314273426792681 2 5 5.961659549365535 2 6 3.394770823194534 2 7 2.660807247066927 2 8 2.846565744652942 2 9 3.0534794291368996 2 10 2.304891709489752 2 11 1.585253394698988 2 12 1.8591726153032724 2 13 -1.5916895467484764 2 14 -0.7314009777716564 2 15 -1.8155660270799245 2 16 -1.3332487992569426 2 17 1.0597304108725254 2 18 1.2761803163256715 2 19 0.9261107828927564 2 20 -2.701665712854421 2 21 -0.028995608106043846 2 22 1.1801631425201862 2 23 5.760221115181825 2 24 4.381694449565721 2 25 4.679211156635795 2 26 5.597932012930304 2 27 4.7807497996528845 2 28 6.114615316287331 2 29 6.576592321570008 2 30 6.795792714073798 2 31 8.441507005683071 2 32 8.78693231883593 2 33 8.661706682363103 2 34 7.6948534255182395 2 35 9.956073374036453 2 36 10.711044080631034 2 37 10.976847945499333 2 38 11.071195821008256 2 39 9.785807716484534 2 40 11.941973903397908 2 41 11.900293510208776 2 42 11.727102671357176 2 43 11.394138354618544 2 44 11.89958621694495 2 45 12.11792775946985 2 46 11.613049901874355 2 47 11.663302837839375 2 48 11.762727035713734 2 49 12.050631672653864 2 50 12.440092596443353 2 51 10.689638757871819 2 52 12.983398918286639 2 53 12.858952180368508 2 54 13.36411783565471 2 55 13.834947932654767 2 56 14.086158318062619 2 57 14.734432964084426 2 58 15.23253815394865 3 1 -2.715320196945534 3 2 -6.6122245257039935 3 3 -6.659096014425277 3 4 -10.685726573207319 3 5 -4.038340450634465 3 6 -2.605229176805466 3 7 -2.839192752933073 3 8 -2.153434255347058 3 9 -3.9465205708631004 3 10 -0.6951082905102481 3 11 3.085253394698988 3 12 5.859172615303272 3 13 5.408310453251524 3 14 4.768599022228344 3 15 5.6844339729200755 3 16 4.666751200743057 3 17 3.5597304108725254 3 18 4.7761803163256715 3 19 5.426110782892756 3 20 5.298334287145579 3 21 5.971004391893956 3 22 7.180163142520186 3 23 5.760221115181825 3 24 7.881694449565721 3 25 4.679211156635795 3 26 6.097932012930304 3 27 2.2807497996528845 3 28 6.614615316287331 3 29 4.576592321570008 3 30 7.295792714073798 3 31 6.441507005683071 3 32 7.78693231883593 3 33 5.6617066823631035 3 34 -4.8051465744817605 3 35 6.456073374036453 3 36 7.211044080631034 3 37 8.476847945499333 3 38 7.571195821008256 3 39 8.785807716484534 3 40 -5.058026096602092 3 41 -3.599706489791224 3 42 -0.7728973286428236 3 43 -0.6058616453814558 3 44 -3.6004137830550498 3 45 -3.3820722405301495 3 46 -1.886950098125645 3 47 -5.836697162160625 3 48 2.762727035713734 3 49 2.5506316726538643 3 50 2.9400925964433533 3 51 11.189638757871819 3 52 11.483398918286639 3 53 11.858952180368508 3 54 12.86411783565471 3 55 12.834947932654767 3 56 12.086158318062619 3 57 13.234432964084426 3 58 6.232538153948649 4 1 -2.381986863612198 4 2 -7.445557859037326 4 3 -4.659096014425277 4 4 -8.352393239873987 4 5 -4.371673783967797 4 6 -3.2718958434721337 4 7 -1.0058594195997372 4 8 -2.820100922013726 4 9 -3.6131872375297682 4 10 -9.861774957176916 4 11 3.9185867280323237 4 12 3.5258392819699367 4 13 4.241643786584859 4 14 1.6019323555616793 4 15 3.3511006395867398 4 16 4.000084534076393 4 17 3.893063744205861 4 18 3.7761803163256715 4 19 3.9261107828927564 4 20 0.46500095381224327 4 21 -2.362328941439376 4 22 2.8468298091868505 4 23 1.0935544485151603 4 24 1.881694449565721 4 25 3.345877823302459 4 26 4.097932012930304 4 27 3.61408313298622 4 28 4.614615316287331 4 29 5.243258988236672 4 30 5.295792714073798 4 31 5.608173672349736 4 32 6.28693231883593 4 33 5.328373349029768 4 34 7.1948534255182395 4 35 8.789406707369789 4 36 6.877710747297698 4 37 8.476847945499333 4 38 9.904529154341592 4 39 7.452474383151198 4 40 8.441973903397908 4 41 10.733626843542112 4 42 9.227102671357176 4 43 7.0608050212852085 4 44 10.39958621694495 4 45 9.951261092803186 4 46 10.113049901874355 4 47 10.163302837839375 4 48 10.262727035713734 4 49 10.550631672653864 4 50 10.273425929776689 4 51 10.856305424538483 4 52 10.483398918286639 4 53 12.525618847035172 4 54 12.197451168988046 4 55 12.001614599321432 4 56 13.752824984729283 4 57 13.90109963075109 4 58 12.399204820615314 5 1 -7.381986863612202 5 2 -5.1122245257039935 5 3 -3.659096014425277 5 4 -4.685726573207319 5 5 -5.371673783967797 5 6 -4.605229176805466 5 7 -4.339192752933073 5 8 -3.820100922013726 5 9 -4.9465205708631 5 10 -0.861774957176916 5 11 -4.08141327196768 5 12 -6.8074940513633955 5 13 -1.0916895467484764 5 14 -3.398067644438324 5 15 -1.6488993604132567 5 16 -8.666582132590275 5 17 -4.773602922460807 5 18 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1 -5.048653530278866 6 2 -11.778891192370661 6 3 -11.325762681091945 6 4 -14.685726573207319 6 5 -12.038340450634465 6 6 -7.271895843472134 6 7 -3.339192752933073 6 8 -2.48676758868039 6 9 -2.2798539041964325 6 10 -7.195108290510248 6 11 2.9185867280323237 6 12 -0.14082738469672762 6 13 -0.0916895467484764 6 14 2.6019323555616793 6 15 1.0177673062534112 6 16 3.000084534076393 6 17 0.5597304108725254 6 18 2.7761803163256715 6 19 2.259444116226092 6 20 2.1316676204789147 6 21 3.971004391893956 6 22 4.180163142520186 6 23 4.760221115181825 6 24 -1.7849722171009468 6 25 4.01254448996913 6 26 -6.568734653736364 6 27 -6.7192502003471155 6 28 -6.052051350379337 6 29 -6.423407678429992 6 30 -5.704207285926202 6 31 -4.725159660983596 6 32 -4.379734347830738 6 33 -4.004959984303564 6 34 -3.4718132411484284 6 35 -3.2105932926302145 6 36 -2.7889559193689664 6 37 -2.1898187211673346 6 38 -2.4288041789917436 6 39 -1.8808589501821338 6 40 -1.2246927632687594 6 41 -1.5997064897912239 6 42 -1.7728973286428236 6 43 -1.6058616453814558 6 44 -1.6004137830550498 6 45 -1.3820722405301495 6 46 -1.553616764792313 6 47 -1.8366971621606254 6 48 -1.7372729642862659 6 49 -1.1160349940128036 6 50 -0.7265740702233145 6 51 -0.810361242128181 6 52 -0.516601081713361 6 53 0.19228551370184022 6 54 -0.13588216434528988 6 55 0.3349479326547673 6 56 1.086158318062619 6 57 1.2344329640844265 6 58 1.7325381539486493 7 1 -0.11532019694553242 7 2 -2.9122245257039943 7 3 -0.059096014425275456 7 4 -1.885726573207318 7 5 -1.238340450634464 7 6 -0.005229176805464419 7 7 -0.1391927529330701 7 8 0.6465657446529391 7 9 0.653479429136901 7 10 -0.7951082905102496 7 11 4.185253394698989 7 12 2.459172615303274 7 13 3.1083104532515264 7 14 2.2685990222283436 7 15 2.4844339729200726 7 16 -0.13324879925693978 7 17 3.5597304108725254 7 18 0.9761803163256744 7 19 0.5261107828927578 7 20 -0.8016657128544225 7 21 2.171004391893959 7 22 3.180163142520186 7 23 0.16022111518182314 7 24 3.081694449565724 7 25 2.8792111566357974 7 26 1.297932012930307 7 27 2.4807497996528873 7 28 2.0146153162873297 7 29 -0.8234076784299909 7 30 1.8957927140737993 7 31 -4.258492994316928 7 32 -1.3130676811640711 7 33 0.6617066823631035 7 34 -2.00514657448176 7 35 2.856073374036452 7 36 -4.188955919368965 7 37 -0.723152054500666 7 38 0.3711958210082571 7 39 -0.4141922835154652 7 40 -1.158026096602093 7 41 0.4002935102087761 7 42 -3.5728973286428243 7 43 4.194138354618545 7 44 4.39958621694495 7 45 4.6179277594698505 7 46 3.113049901874355 7 47 3.1633028378393746 7 48 3.4627270357137334 7 49 2.1506316726538657 7 50 3.1400925964433526 7 51 2.5896387578718176 7 52 3.2833989182866397 7 53 -0.14104781963149193 7 54 3.2641178356547087 7 55 4.134947932654768 7 56 3.2861583180626184 7 57 -1.165567035915572 7 58 -0.2674618460513507 8 1 -8.315320196945535 8 2 -9.112224525703994 8 3 -10.059096014425275 8 4 -9.28572657320732 8 5 -10.438340450634463 8 6 -9.205229176805467 8 7 -9.539192752933072 8 8 -10.353434255347057 8 9 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2.798334287145579 120 21 2.971004391893956 120 22 3.180163142520186 120 23 3.7602211151818246 120 24 3.881694449565721 120 25 3.6792111566357946 120 26 4.097932012930304 120 27 4.2807497996528845 120 28 4.614615316287331 120 29 4.576592321570008 120 30 5.295792714073798 120 31 5.941507005683071 120 32 6.28693231883593 120 33 6.6617066823631035 120 34 7.1948534255182395 120 35 7.456073374036453 120 36 8.211044080631034 120 37 8.476847945499333 120 38 8.571195821008256 120 39 8.785807716484534 120 40 9.441973903397908 120 41 9.400293510208776 120 42 9.227102671357176 120 43 9.394138354618544 120 44 9.39958621694495 120 45 9.61792775946985 120 46 9.113049901874355 120 47 9.163302837839375 120 48 9.262727035713734 120 49 9.550631672653864 120 50 9.940092596443353 120 51 10.189638757871819 120 52 10.483398918286639 120 53 10.858952180368508 120 54 10.86411783565471 120 55 11.334947932654767 120 56 12.086158318062619 120 57 12.234432964084426 120 58 12.73253815394865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 820.0 3 26.0 4 149.0 5 138.0 6 319.0 7 463.0 8 223.0 9 416.0 10 409.0 11 437.0 12 476.0 13 572.0 14 638.0 15 787.0 16 913.0 17 1000.0 18 1070.0 19 1277.0 20 1230.0 21 1195.0 22 1373.0 23 1606.0 24 1710.0 25 1514.0 26 1814.0 27 1943.0 28 2167.0 29 2741.0 30 3203.0 31 3529.0 32 4072.0 33 4722.0 34 5293.0 35 5528.0 36 6287.0 37 8748.0 38 9296.0 39 5354.0 40 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 37.503890817670296 46.262420687178256 7.7253681312103435 8.508320363941099 11 22.52124985035317 49.48401771818508 16.726924458278464 11.267807973183288 12 45.60038309589369 26.886148689093737 16.301927451215132 11.211540763797439 13 15.663833353286243 31.223512510475278 23.467017837902553 29.645636298335926 14 21.252244702502097 46.740093379624085 18.41374356518616 13.593918352687655 15 31.04633065964324 30.003591524003355 20.98288040225069 17.967197414102717 16 17.93247934873698 34.43912366814318 33.0958936908895 14.532503292230336 17 36.69699509158386 27.223751945408836 16.828684305040106 19.2505686579672 18 30.442954627080095 28.93930324434335 21.207949239794086 19.409792888782473 19 22.278490105232912 28.679859689449174 33.330938955332876 15.710711249985035 20 16.982125968226605 39.495265117504104 23.774976355517246 19.747632558752052 21 17.794033138588258 31.82405899818025 33.087108514510106 17.294799348721387 22 24.4079971267808 32.497306356997484 19.45408835149048 23.640608164731233 23 21.243430268057033 47.45291941527889 17.86129036120057 13.442359955463504 24 22.439841972943853 41.15168203040824 19.669579791691607 16.7388962049563 25 19.918592122590688 32.90434574404406 20.98647192625404 26.19059020711122 26 31.88115966326176 31.25127234842588 16.306417426084042 20.561150562228317 27 28.409798381303574 28.586992960107278 17.949331928547483 25.05387673004166 28 27.16908374549417 41.36138821359712 18.39694382237764 13.072584218531071 29 32.26165672821323 33.386019110568284 21.664631079818953 12.687693081399527 30 19.95423834395515 45.20940150462408 22.838899803536343 11.997460347884422 31 26.09758142766444 39.87709483822279 23.14953461349561 10.875789120617162 32 18.477623022478774 39.06061005257422 30.719392582124765 11.742374342822242 33 22.678449545568846 37.231023457987575 23.207721138532648 16.882805857910935 34 19.86108616250524 38.28872522603437 24.80330519130591 17.046883420154483 35 19.507784431137726 44.92095808383233 24.948502994011974 10.622754491017965 36 20.65338492686258 39.59963101841314 23.708264947947242 16.03871910677704 37 17.89732223087522 37.31024980530761 31.390403163002457 13.402024800814713 38 19.677651288196525 36.256440982624326 29.37567405632115 14.690233672858 39 18.82630626744785 36.12738578771432 27.85306063764782 17.193247307190013 40 19.002947307885844 36.519061653847075 31.430762226535357 13.047228811731722 41 16.867975171951016 35.83770700026362 34.97016320368107 12.324154624104297 42 19.787871524448704 36.585570469798654 30.165388302972197 13.46116970278044 43 20.229876791792513 35.19583872668872 29.45491154897167 15.119372932547101 44 17.925704014379868 36.42061114439784 32.795686039544634 12.85799880167765 45 19.39059167485442 37.38646984111769 29.181145007069425 14.04179347695847 46 20.81580807899246 37.92045631568225 27.74442487208062 13.519310733244657 47 20.206197926032488 38.32763891386441 27.79236348378589 13.673799676317207 48 19.94869274385931 38.11361919946295 28.248960069049016 13.688727987628718 49 18.425438543902352 39.08826033260992 28.195105574273686 14.29119554921404 50 15.939422281377027 39.000203844262984 30.78528004604482 14.275093828315166 51 16.796748552314497 39.740315793259725 29.767770864055436 13.695164790370344 52 17.384477075217262 39.862151633203474 29.838777344920587 12.914593946658675 53 15.062054079980813 39.68583248396187 31.411955153186643 13.840158282870677 54 15.752299408809103 40.3856531280354 28.41073976808049 15.451307695075009 55 18.869418156957288 38.62281553980305 28.59079078358701 13.916975519652643 56 17.0071617941674 40.91099941218105 28.983073213450254 13.098765580201299 57 17.894547417671404 39.27418871093516 28.850098974266693 13.981164897126746 58 18.232753449310138 37.552489502099576 29.29574085182963 14.919016196760648 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2143.5 10 4287.0 11 2681.0 12 1075.0 13 706.5 14 338.0 15 374.0 16 525.5 17 641.0 18 807.0 19 973.0 20 1041.5 21 1110.0 22 1175.5 23 1389.5 24 1538.0 25 1785.0 26 2032.0 27 2233.5 28 2894.5 29 3354.0 30 4011.0 31 4668.0 32 4420.0 33 4172.0 34 4591.0 35 3743.5 36 2477.0 37 2257.5 38 2038.0 39 1931.5 40 1825.0 41 1848.0 42 1841.0 43 1811.0 44 2222.0 45 2633.0 46 2971.5 47 2788.5 48 2267.0 49 2408.0 50 2549.0 51 2733.5 52 2918.0 53 3633.5 54 3967.0 55 3585.0 56 3472.0 57 3359.0 58 3394.0 59 3486.5 60 3544.0 61 3094.5 62 2645.0 63 2207.0 64 1769.0 65 1614.5 66 1161.0 67 862.0 68 705.0 69 548.0 70 451.5 71 355.0 72 310.5 73 222.0 74 178.0 75 130.5 76 83.0 77 72.0 78 43.0 79 25.0 80 20.0 81 15.0 82 11.0 83 7.0 84 4.5 85 2.0 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0011971746677840295 20 0.0011971746677840295 21 0.002394349335568059 22 0.0 23 0.003591524003352089 24 0.0 25 0.0 26 0.027535017359032684 27 0.007183048006704178 28 0.0323237160301688 29 0.019154794684544472 30 0.0646474320603376 31 0.06105590805698551 32 0.034718065365736864 33 0.022746318687896564 34 0.029929366694600744 35 0.03591524003352089 36 0.0682389560636897 37 0.07781635340596194 38 0.09577397342272237 39 0.08021070274152999 40 0.07661917873817789 41 0.09098527475158626 42 0.10774572010056266 43 0.11253441877169879 44 0.09577397342272237 45 0.08619657608045013 46 0.09457679875493834 47 0.1376750867951634 48 0.1328863881240273 49 0.15443553214413983 50 0.15922423081527595 51 0.1448581348018676 52 0.12570334011732312 53 0.16161858015084402 54 0.16640727882198011 55 0.18795642284209266 56 0.2047168681910691 57 0.2095055668622052 58 0.21549144020112532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 83530.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.94588770501616 #Duplication Level Percentage of deduplicated Percentage of total 1 81.7158962295555 44.082365617143545 2 9.784514325026075 10.556686220519573 3 3.484165908435232 5.63869268526278 4 1.6666296797674265 3.596312702023225 5 0.9409467166729545 2.538010295702143 6 0.5725572002396752 1.8532263857296778 7 0.36838951643327933 1.3911169639650425 8 0.3062515257095937 1.3216808332335688 9 0.186413972171057 0.9050640488447264 >10 0.8677126561771821 8.418532263857296 >50 0.05326113490601629 1.9154794684544476 >100 0.033288209316260184 3.1892733149766554 >500 0.008876855817669381 3.056386926852628 >1k 0.011096069772086726 11.537172273434694 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4027 4.821022387166288 No Hit TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 2279 2.7283610678798036 No Hit TAGTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1239 1.4832994133844126 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1085 1.2989345145456723 No Hit TAGTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1007 1.2055548904585178 No Hit TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 772 0.924218843529271 No Hit TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 632 0.7566143900395067 No Hit TAGTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 603 0.7218963246737699 No Hit TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 546 0.6536573686100803 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 354 0.4237998323955465 No Hit TAGTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 307 0.3675326230096971 No Hit TAGTCGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 307 0.3675326230096971 No Hit TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.241829282892374 No Hit TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.22866036154674968 No Hit TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 190 0.22746318687896566 No Hit TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 161 0.19274512151322878 Illumina Single End Adapter 2 (96% over 25bp) TAGTCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 137 0.16401292948641208 No Hit TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 0.15443553214413983 No Hit TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 123 0.14725248413743564 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 120 0.14366096013408355 No Hit TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 119 0.14246378546629954 No Hit TAGTCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 111 0.1328863881240273 Illumina Single End Adapter 2 (96% over 32bp) TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 107 0.12809768945289118 No Hit TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.12690051478510717 No Hit TAGTCGGGGTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGGTTC 99 0.11852029211061893 No Hit TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 98 0.1173231174428349 No Hit TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92 0.11014006943613074 No Hit TAGTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 87 0.10415419609721059 No Hit TAGTCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC 86 0.10295702142942656 Illumina Single End Adapter 2 (100% over 31bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.031126541362384772 0.0 0.0 0.0 0.0 11 0.08619657608045014 0.0 0.0 0.0 0.0 12 0.08859092541601819 0.0 0.0 0.0 0.0 13 0.0969711480905064 0.0 0.0 0.0 0.0 14 0.16760445348976416 0.0 0.0 0.0 0.0 15 0.1723931521609003 0.0 0.0 0.0 0.0 16 0.1819705495031725 0.0 0.0 0.0 0.0 17 0.1891535975098767 0.0 0.0 0.0 0.0 18 0.19154794684544474 0.0 0.0 0.0 0.0 19 0.202322518855501 0.0 0.0 0.0 0.0 20 0.2454208068957261 0.0 0.0 0.0 0.0 21 0.2538010295702143 0.0 0.0 0.0 0.0 22 0.2573925535735664 0.0 0.0 0.0 0.0 23 0.30049084161379147 0.0 0.0 0.0 0.0 24 0.33880043098288043 0.0 0.0 0.0 0.0 25 0.3471806536573686 0.0 0.0 0.0 0.0 26 0.362743924338561 0.0 0.0 0.0 0.0 27 0.37711002035196933 0.0 0.0 0.0 0.0 28 0.5147851071471328 0.0 0.0 0.0 0.0 29 0.524362504489405 0.0 0.0 0.0 0.0 30 0.5842212378786065 0.0 0.0 0.0 0.0 31 0.5902071112175267 0.0 0.0 0.0 0.0 32 0.5961929845564468 0.0 0.0 0.0 0.0 33 0.602178857895367 0.0 0.0 0.0 0.0 34 0.608164731234287 0.0 0.0 0.0 0.0 35 0.8356279181132528 0.0 0.0 0.0 0.0 36 0.8428109661199569 0.0 0.0 0.0 0.0 37 0.847599664791093 0.0 0.0 0.0 0.0 38 0.8655572848078534 0.0 0.0 0.0 0.0 39 0.887106428827966 0.0 0.0 0.0 0.0 40 0.9062612235125105 0.0 0.0 0.0 0.0 41 0.9218244941937028 0.0 0.0 0.0 0.0 42 0.9349934155393271 0.0 0.0 0.0 0.0 43 0.9505566862205196 0.0 0.0 0.0 0.0 44 0.9661199569017119 0.0 0.0 0.0 0.0 45 0.9876691009218245 0.0 0.0 0.0 0.0 46 1.005626720938585 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 15 0.007180448 52.029957 51 ATTAAGC 25 1.6363063E-5 52.029957 51 TTAAGCC 25 1.6363063E-5 52.029957 52 CGTTCCT 15 0.0071974895 51.998806 18 ACAAATT 15 0.0071974895 51.998806 26 GGATGTG 15 0.0071974895 51.998806 8 TGGTTAG 15 0.0071974895 51.998806 37 GGGTTTA 15 0.0071974895 51.998806 8 AAACTCT 15 0.0071974895 51.998806 34 CCAACGC 15 0.0071974895 51.998806 21 GGGTCAG 15 0.0071974895 51.998806 7 GGCACCC 30 7.9067104E-7 51.998806 37 TTCGCTA 15 0.0071974895 51.998806 32 TTCCGTT 15 0.0071974895 51.998806 15 TTTCCGT 15 0.0071974895 51.998806 14 GTTCCTA 15 0.0071974895 51.998806 19 AAGAGTG 15 0.0071974895 51.998806 10 GGATTTC 15 0.0071974895 51.998806 8 GGATCGC 15 0.0071974895 51.998806 35 GTCGAGT 15 0.0071974895 51.998806 31 >>END_MODULE