FastQCFastQC Report
Tue 31 May 2016
SRR212611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88741
Sequences flagged as poor quality0
Sequence length58
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43134.860211176344643No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT25062.823948344057426No Hit
TAGTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC12781.4401460429790063No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11381.2823835656573623No Hit
TAGTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10341.1651885825041413No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8420.9488286136058868No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.7459911427637732No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5620.6333036589625991No Hit
TAGTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5360.6040049131742937No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.454130559718732No Hit
TAGTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3140.353838699135687No Hit
TAGTCGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3020.3403162010795461No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.24227809017252455No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.23777059082047755No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.20847184503217228No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1580.17804622440585524Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.16114310183567912No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1370.15438185280760866Illumina Single End Adapter 2 (96% over 32bp)
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.15212810313158517No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.13522498056140905No Hit
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1150.12959060637135034No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1140.1284637315333386No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.12508310701930336No Hit
TAGTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1070.1205756076672564No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1050.1183218579912329No Hit
TAGTCGGGGTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGGTTC1030.11606810831520943No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.11381435863918594No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.11043373412515073No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA970.10930685928713897No Hit
TAGTCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA930.10479935993509201No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.10254561025906853No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.10029186058304505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTCT150.007168702352.05417649
CAGACTA150.007168702352.05417649
GATTAAG150.007168702352.05417650
AGATTAA150.007168702352.05417649
ACTACCC150.007168702352.05417652
GAGGTTC251.6326328E-552.05417652
GTAGATC150.00720077551.9954918
ACAAATT251.6435835E-551.9954926
ATCCTGC150.00720077551.9954920
TTGTCTC150.00720077551.9954940
CGCGAAC203.4317974E-451.9954925
GGATGGA401.8626451E-951.995498
TTGTTAA150.00720077551.9954931
ACGCTAC150.00720077551.9954941
GCTAGGC150.00720077551.9954939
AACGAGC251.6435835E-551.9954935
CCAGTAG150.00720077551.9954926
GGCGTGA307.9158417E-751.995499
CAGTAGC150.00720077551.9954927
GGCAAAC150.00720077551.9954936
Produced by FastQC (version 0.11.5)