FastQCFastQC Report
Tue 31 May 2016
SRR212610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93433
Sequences flagged as poor quality0
Sequence length58
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38764.148427215223744No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT22162.371753020881273No Hit
TAGTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11911.2747102201577603No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10291.101323943360483No Hit
TAGTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9811.0499502317168452No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6910.7395673905365342No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.6421713955454711No Hit
TAGTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5560.5950788265388032No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5010.5362131152804683No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.3649674098016761No Hit
TAGTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3320.35533483886849404No Hit
TAGTCGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3130.3349994113428874No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.22368970278167244No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.22261941712243No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.2044245609153083No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1720.1840891333897017Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1260.13485599306454893No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.12950456476833666No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.1262937077906093No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.12522342213136686No Hit
TAGTCGGGGTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGGTTC1090.11666113685742725No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1070.11452056553894234No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.10916913724273009No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.10916913724273009No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.10702856592424519No Hit
TAGTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA970.10381770894651783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTC150.007034212452.3059927
GGACTAA150.007034212452.3059928
GAAGCAC150.007034212452.3059929
GAACAGT503.6379788E-1252.3059929
GGCTACG150.007034212452.3059928
GGTGGAA150.007034212452.3059928
GGTGTTT150.007034212452.3059929
GGAGTAG150.007034212452.3059928
GAGACAG150.007034212452.30599210
GACTAAT150.007034212452.3059929
GGAATGA203.3331802E-452.305998
AAACTGG203.4139506E-452.0531739
TATACTA150.00717038952.0531744
CAATTAT251.6334676E-552.05316544
GTAGAGC150.0071856452.0252340
TATATAA150.0071856452.0252352
CCGCTCC150.0071856452.0252351
CGCTCCG150.0071856452.0252352
TTATATA150.0071856452.0252351
GCCGCTC150.0071856452.0252350
Produced by FastQC (version 0.11.5)