SRR212609_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212609_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	175261
Sequences flagged as poor quality	0
Sequence length	56
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	8307	4.739788087480957	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5932	3.3846662976931547	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	2282	1.3020580733876903	No Hit
TAGTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1587	0.905506644376102	No Hit
TAGTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1521	0.8678485230598936	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1299	0.7411802968144653	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1196	0.6824108044573521	No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	946	0.5397664055323204	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	753	0.4296449295621958	No Hit
TAGTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	631	0.3600344628867803	No Hit
TAGTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	467	0.2664597371919594	No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	454	0.25904222844785774	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	438	0.24991298691665575	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	397	0.2265193054929505	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	368	0.20997255521764685	Illumina Single End Adapter 2 (95% over 23bp)
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	357	0.20369620166494543	No Hit
TAGTCGGGGCTTTTGGACCAGGGAGAGCATAAGGGCAGAATGTAGCTGCTAGAGTT	316	0.18030252024124022	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	295	0.16832039073153754	Illumina Single End Adapter 2 (96% over 30bp)
TAGTCGGGGTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGGT	288	0.16432634756163664	No Hit
TAGTCGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	272	0.1551971060304346	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGGAGCAGG	263	0.15006190766913347	No Hit
TAGTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	244	0.13922093335083105	No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	237	0.13522689018093015	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA	222	0.12666822624542826	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	222	0.12666822624542826	No Hit
TAGTCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	219	0.12495649345832788	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAGCAGT	205	0.11696840711852609	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAGCAGG	199	0.11354494154432532	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	194	0.11069205356582469	No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	192	0.10955089837442443	No Hit
TAGTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	192	0.10955089837442443	No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	183	0.1044157000131233	No Hit
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	182	0.10384512241742316	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	177	0.10099223443892251	Illumina Single End Adapter 2 (100% over 29bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAGT	20	4.07245E-4	50.25029	49
GCTAGAG	25	2.0195639E-5	50.25029	48
AGACTAC	15	0.008254519	50.250286	50
ATAGAGG	30	1.0074127E-6	50.250286	49
TCAGACT	15	0.008254519	50.250286	48
ACGGTGA	15	0.008254519	50.250286	48
AACATAG	30	1.01142E-6	50.22139	46
ACATAGA	30	1.01142E-6	50.22139	47
CGAATAA	25	2.03682E-5	50.178112	44
CCGAATA	25	2.03682E-5	50.178112	43
GGGCGTA	15	0.008301567	50.17811	45
CGCTACT	15	0.008301567	50.17811	42
TGGGGTT	15	0.008301567	50.17811	45
TACTCAG	15	0.008301567	50.17811	45
ATGAAGT	15	0.008301567	50.17811	45
CTCGTCG	20	4.1072702E-4	50.1637	40
TCGGCGG	20	4.1072702E-4	50.1637	41
TGTAGCT	25	2.0402858E-5	50.1637	41
AATGTAG	25	2.0402858E-5	50.1637	39
TAGAGCT	15	0.008311001	50.163696	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content