SRR212608_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212608_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	161650
Sequences flagged as poor quality	0
Sequence length	56
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	7436	4.6000618620476335	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5286	3.270027837921435	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	2076	1.284256108877204	No Hit
TAGTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1619	1.0015465511908443	No Hit
TAGTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1575	0.9743272502319827	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1207	0.7466749149396845	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1170	0.7237859573151871	No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	751	0.46458397772966287	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	726	0.44911846582121867	No Hit
TAGTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	674	0.4169502010516548	No Hit
TAGTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	451	0.27899783482833285	No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.2697185276832663	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	418	0.2585833591091865	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	385	0.23816888339004022	No Hit
TAGTCGGGGCTTTTGGACCAGGGAGAGCATAAGGGCAGAATGTAGCTGCTAGAGTT	353	0.2183730281472317	No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	317	0.19610269099907207	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	299	0.18496752242499226	Illumina Single End Adapter 2 (95% over 23bp)
TAGTCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	299	0.18496752242499226	Illumina Single End Adapter 2 (96% over 30bp)
TAGTCGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	277	0.1713578719455614	No Hit
TAGTCGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	266	0.16455304670584597	No Hit
TAGTCGGGGTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGAGGT	256	0.1583668419424683	No Hit
TAGTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	241	0.14908753479740178	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	219	0.13547788431797092	No Hit
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	211	0.13052892050726878	No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	199	0.12310547479121559	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA	199	0.12310547479121559	No Hit
TAGTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	190	0.1175378905041757	No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	185	0.11444478812248685	No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	183	0.11320754716981132	No Hit
TAGTCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	177	0.10949582431178473	No Hit
TAGTCGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	173	0.10702134240643366	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGGAGCAGG	172	0.10640272193009588	No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA	168	0.10392824002474481	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	165	0.10207237859573151	Illumina Single End Adapter 2 (100% over 29bp)
TAGTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	162	0.10021651716671821	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAGT	25	2.015531E-5	50.264275	49
ATTACCT	15	0.008244276	50.264275	50
TAAACTG	20	4.065903E-4	50.264275	49
CGCGCGA	15	0.008244276	50.264275	49
ATAGAGG	20	4.065903E-4	50.264275	49
TCAGCTC	15	0.008244276	50.264275	50
AGATTAA	40	2.5374902E-9	50.264275	49
GATTAAG	40	2.5374902E-9	50.264275	50
CTGCTAC	15	0.008264638	50.23293	43
ATGGGGG	15	0.008264638	50.23293	44
ACTGCTA	15	0.008264638	50.23293	42
TGTGCAA	15	0.008264638	50.23293	43
CATTATA	15	0.008264638	50.23293	47
AACATAG	20	4.0784443E-4	50.23293	46
CATAGAG	20	4.0784443E-4	50.23293	48
TGTTCAT	15	0.008264638	50.23293	43
AGCGCGT	15	0.008264638	50.23293	44
TCACTAA	15	0.008264638	50.23293	45
ATTGGGT	15	0.008264638	50.23293	43
TGCTATC	15	0.008264638	50.23293	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content