FastQCFastQC Report
Tue 31 May 2016
SRR212606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199691
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT42942.150322247872964No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC40262.016114897516663No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC35981.80178375590287No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT29771.4908032910847258No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA18690.935946036626588No Hit
ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG17860.8943818199117637No Hit
ATCAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC16760.8392967134222374No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT16000.8012379125749283No Hit
ATCAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG13730.6875622837283604No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13430.6725390728675804No Hit
ATCAACGGGGCCCAACTTCACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACC12670.6344802720202714No Hit
ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG12160.6089408135569455No Hit
ATCAACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT10580.5298185696901713No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA7780.38960193498955886No Hit
ATCAACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT6740.3375214706721886No Hit
ATCAACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTT6410.3209959387253306No Hit
ATCAACGGGATCTCAGCCTACTAGAAACCAGTCTGGGATGGACATGATGGGCTTCTGT6210.310980464818144No Hit
ATCAACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5690.28494023265945884No Hit
ATCAACGGGAAGTGGAGTATATTGAATATTGAAGTAGACTTCAGGTTGTTTTTTGGTT5680.28443945896409956No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5240.262405416368289No Hit
ATCAACGGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCTA5190.25990154789149233No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA5100.2553945846332584No Hit
ATCAACGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5060.2533914898518211No Hit
ATCAACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC4790.23987060007711913No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4680.2343620894281665No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCTA4590.22985512616993256No Hit
ATCAACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4380.2193388785673866No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA4100.20531721509732537No Hit
ATCAACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4080.20431566770660672No Hit
ATCAACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA4070.20381489401124736No Hit
ATCAACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG4050.20281334662052872No Hit
ATCAACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC3880.1943001937994201No Hit
ATCAACGGGGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTGCCA3800.19029400423654547No Hit
ATCAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3680.1842847198922335No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA3610.1807793040247182No Hit
ATCAACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT3530.17677311446184354No Hit
ATCAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA3370.1687607353360943No Hit
ATCAACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT3350.16775918794537562No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG3270.16375299838250096No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.16375299838250096No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC3240.16225067729642295No Hit
ATCAACGGGATTGTCCTCTCTGGTGGTTCAACCATGTTCAGGGACTTTGGACGTCGTT3220.1612491299057043No Hit
ATCAACGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG3170.15874526142890766No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCGAA3060.15323675077995502No Hit
ATCAACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2970.14872978752172106No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTTCCGA2960.14822901382636175No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGTGGTTAAGAGCACTGACTGCTCTTCTG2930.14672669274028374No Hit
ATCAACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2910.1457251453495651No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAA2900.14522437165420574No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA2870.14372205056812776No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA2860.14322127687276842No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC2800.14021663470061244No Hit
ATCAACGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2760.13821353991917512No Hit
ATCAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2760.13821353991917512No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTGAGAGCACTGACTGCTCTTCCAGA2730.13671121883309714No Hit
ATCAACGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG2700.13520889774701914No Hit
ATCAACGGGAGTCTTTTGTATTTTTGATTATTAAGTAAAACTTGCTTTTATTTTAATA2670.13370657666094116No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGA2630.13170348187950384No Hit
ATCAACGGGATGGAGCTCGCGCTTCCCGACTTCCTCAAGGCCTTCTACGAGTGCCTGG2580.1291996134027072No Hit
ATCAACGGGAACTTTAAACGATAAAAGTACTATCCAATAGGAGATTTATTCTTTCATA2580.1291996134027072No Hit
ATCAACGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA2560.12819806601198852No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2540.12719651862126985No Hit
ATCAACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG2510.12569419753519187No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTCCA2500.12519342383983256No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGA2370.11868336580016124No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC2370.11868336580016124No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC2360.11818259210480192No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTCT2250.11267408145584928No Hit
ATCAACGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA2240.11217330776048995No Hit
ATCAACGGGGGGCTAGAGAGATGGCTCAGCAGTTAAGAGCACTGACTTCTCTTCCAGA2240.11217330776048995No Hit
ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCAACTGCTCTTCCAAA2220.1111717603697713No Hit
ATCAACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2210.11067098667441196No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2180.10916866558833398No Hit
ATCAACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.1081671181976153No Hit
ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAA2110.10566324972081866No Hit
ATCAACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA2100.10516247602545933No Hit
ATCAACGGGGGGGCTGGAGAAATAGCTCAGCAGTTAAGAGCTCTGACTGCTCTTCCAG2100.10516247602545933No Hit
ATCAACGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT2060.103159381244022No Hit
ATCAACGGGATTGACTGTAGGAAAAGTTTCCTGTTGACCCTTGGGTAGTGAGGGCTGA2020.10115628646258469No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA2020.10115628646258469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC203.389857E-452.15715427
GAATATC203.389857E-452.15715424
AAAGTAC401.8317223E-952.15715424
GTACTAT401.8317223E-952.15715427
ATTATTA251.6210028E-552.15715427
GCAAGGT150.007125871752.1571524
AGAACTT150.007125871752.1571524
ACAGGAT150.007125871752.1571525
TATCTAT150.007125871752.1571524
CAAGGTT150.007125871752.1571525
GTCACTC150.007125871752.1571526
CTATTAG150.007125871752.1571527
CCGGTGC150.007125871752.1571526
GTTATCT150.007125871752.1571525
TAACTGC150.007125871752.1571527
TCTATTA150.007125871752.1571526
GTGCCAA307.797462E-752.1571526
AAGTCAC150.007125871752.1571524
AAAAGTA401.8408173E-952.13093623
TTATCTA150.00714005752.13093623
Produced by FastQC (version 0.11.5)