FastQCFastQC Report
Tue 31 May 2016
SRR212605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198650
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT41322.0800402718348856No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC38901.9582179713063177No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC35321.7780015101938083No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT28831.4512962496853763No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA16650.8381575635539894No Hit
ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG15900.8004027183488548No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT14700.7399949660206393No Hit
ATCAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC13880.6987163352630255No Hit
ATCAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG12450.6267304304052354No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG11750.5914925748804429No Hit
ATCAACGGGGCCCAACTTCACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACC11510.5794110244147999No Hit
ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG11380.5728668512459099No Hit
ATCAACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT9870.4968537628995721No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA7990.4022149509187012No Hit
ATCAACGGGATCTCAGCCTACTAGAAACCAGTCTGGGATGGACATGATGGGCTTCTGT6200.3121067203624465No Hit
ATCAACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTT6010.30254215957714575No Hit
ATCAACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5950.29952177196073493No Hit
ATCAACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT5770.2904606091115026No Hit
ATCAACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC5150.259249937075258No Hit
ATCAACGGGAAGTGGAGTATATTGAATATTGAAGTAGACTTCAGGTTGTTTTTTGGTT5090.25622954945884724No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4780.2406242134407249No Hit
ATCAACGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4590.2310596526554241No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA4510.22703246916687642No Hit
ATCAACGGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCTA4500.22652907123080793No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCTA4250.2139441228290964No Hit
ATCAACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4170.20991693934054872No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA3890.19582179713063175No Hit
ATCAACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3870.19481500125849482No Hit
ATCAACGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG3850.19380820538635793No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3790.19078781776994713No Hit
ATCAACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3680.18525044047319406No Hit
ATCAACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA3620.1822300528567833No Hit
ATCAACGGGGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTGCCA3440.17316889000755098No Hit
ATCAACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT3430.17266549207148252No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG3420.17216209413541403No Hit
ATCAACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC3390.17065190032720867No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA3320.1671281147747294No Hit
ATCAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3320.1671281147747294No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCGAA3050.15353637050088095No Hit
ATCAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA2930.1474955952680594No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA2910.1464887993959225No Hit
ATCAACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2880.1449786055877171No Hit
ATCAACGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA2850.1434684117795117No Hit
ATCAACGGGATTGTCCTCTCTGGTGGTTCAACCATGTTCAGGGACTTTGGACGTCGTT2840.14296501384344326No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.139441228290964No Hit
ATCAACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2750.13843443241882708No Hit
ATCAACGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA2680.13491064686634785No Hit
ATCAACGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2620.13189025924993708No Hit
ATCAACGGGAGTCTTTTGTATTTTTGATTATTAAGTAAAACTTGCTTTTATTTTAATA2520.12685627988925247No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGTGGTTAAGAGCACTGACTGCTCTTCTG2500.12584948401711551No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTTCCGA2490.12534608608104708No Hit
ATCAACGGGATGGAGCTCGCGCTTCCCGACTTCCTCAAGGCCTTCTACGAGTGCCTGG2450.12333249433677324No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAA2450.12333249433677324No Hit
ATCAACGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG2420.12182230052856784No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA2390.12031210672036245No Hit
ATCAACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2340.11779511704002013No Hit
ATCAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2330.11729171910395168No Hit
ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCAACTGCTCTTCCAAA2320.11678832116788322No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTGAGAGCACTGACTGCTCTTCCAGA2280.11477472942360936No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA2260.11376793355147244No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC2240.11276113767933552No Hit
ATCAACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2200.11074754593506167No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2190.11024414799899321No Hit
ATCAACGGGAACTTTAAACGATAAAAGTACTATCCAATAGGAGATTTATTCTTTCATA2150.10823055625471935No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGA2140.1077271583186509No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTCCA2140.1077271583186509No Hit
ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAA2140.1077271583186509No Hit
ATCAACGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT2120.10672036244651396No Hit
ATCAACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA2100.10571356657437705No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2060.10369997483010321No Hit
ATCAACGGGGGGCTAGAGAGATGGCTCAGCAGTTAAGAGCACTGACTTCTCTTCCAGA2050.10319657689403473No Hit
ATCAACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG2020.10168638308582933No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGA2000.10067958721369243No Hit
ATCAACGGGGGGGCTGGAGAAATAGCTCAGCAGTTAAGAGCTCTGACTGCTCTTCCAG2000.10067958721369243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA353.6816346E-852.3156436
CGGGTAC353.6816346E-852.3156436
GGATCTA353.6816346E-852.3156438
ATTGTCC251.592098E-552.31564310
GATTGTT203.339384E-452.3156439
GATTGTC251.592098E-552.3156439
GGATTGT503.6379788E-1252.3156438
GAGATTC251.592098E-552.3156439
AAGTACA150.0070407952.3156410
GGGTTAT150.0070407952.315647
GATCTAT150.0070407952.315649
TGGGAAT307.6355354E-752.3156410
GGTTATC150.0070407952.315648
TGGACAT1200.052.0644439
GCGCTGA150.007176124552.0644439
TCTGCGA150.00719048252.0381427
GATACGT150.00719048252.0381425
GATGTAC150.00719048252.0381428
ATCAGCT251.6430391E-552.0381425
TGCGGTC203.428261E-452.0381426
Produced by FastQC (version 0.11.5)