##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 196716 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.97889343012261 39.0 38.0 40.0 35.0 40.0 2 37.90269728949348 39.0 38.0 40.0 35.0 40.0 3 37.82160068321845 39.0 38.0 40.0 35.0 40.0 4 37.91134427296203 39.0 38.0 40.0 35.0 40.0 5 38.08974358974359 39.0 38.0 40.0 35.0 40.0 6 37.76986111958356 39.0 38.0 40.0 35.0 40.0 7 38.186634539132555 39.0 38.0 40.0 35.0 40.0 8 38.1585331137274 39.0 38.0 40.0 35.0 40.0 9 38.10976229691535 39.0 38.0 40.0 35.0 40.0 10 36.97098354988918 39.0 36.0 39.0 33.0 40.0 11 36.91599564854918 39.0 36.0 39.0 33.0 40.0 12 36.70525020842229 39.0 35.0 39.0 31.0 40.0 13 36.71569674047866 39.0 35.0 39.0 31.0 40.0 14 36.7678073974664 38.0 36.0 39.0 32.0 40.0 15 36.86796193497224 38.0 36.0 39.0 32.0 40.0 16 36.60818133756278 38.0 36.0 39.0 32.0 40.0 17 36.18136806360438 38.0 35.0 39.0 30.0 40.0 18 35.99558754753045 38.0 35.0 39.0 30.0 40.0 19 35.82134142621851 38.0 35.0 39.0 29.0 40.0 20 35.733148295003964 38.0 35.0 39.0 29.0 40.0 21 35.51571300758454 38.0 35.0 39.0 29.0 40.0 22 35.39157465584904 38.0 35.0 39.0 29.0 40.0 23 35.06556152016105 38.0 34.0 39.0 28.0 40.0 24 34.762149494703024 38.0 33.0 39.0 27.0 40.0 25 34.66015474084467 38.0 33.0 39.0 27.0 40.0 26 33.95192053518778 37.0 33.0 39.0 26.0 40.0 27 33.65304804896399 36.0 33.0 38.0 25.0 40.0 28 33.16268122572643 36.0 32.0 38.0 23.0 40.0 29 33.21776571300759 36.0 32.0 38.0 23.0 40.0 30 32.87056975538339 36.0 32.0 38.0 23.0 40.0 31 31.570970332865652 35.0 30.0 38.0 17.0 39.0 32 31.652056772199515 35.0 30.0 38.0 17.0 39.0 33 30.93044795542813 35.0 29.0 38.0 13.0 39.0 34 30.72189349112426 35.0 29.0 38.0 11.0 39.0 35 30.435648345838672 35.0 29.0 38.0 9.0 39.0 36 30.42886699607556 35.0 29.0 38.0 2.0 39.0 37 30.170169177901137 35.0 29.0 38.0 2.0 39.0 38 29.865145692267024 35.0 29.0 38.0 2.0 39.0 39 29.733890481709672 35.0 28.0 38.0 2.0 39.0 40 29.662635474491143 35.0 28.0 38.0 2.0 39.0 41 28.833953516744952 34.0 26.0 38.0 2.0 39.0 42 28.410398747432847 34.0 25.0 38.0 2.0 39.0 43 28.44413265824844 34.0 26.0 38.0 2.0 39.0 44 28.169177901136663 34.0 25.0 38.0 2.0 39.0 45 27.45370991683442 33.0 23.0 38.0 2.0 39.0 46 26.473001687712234 33.0 18.0 37.0 2.0 39.0 47 26.436842961426624 33.0 19.0 37.0 2.0 39.0 48 26.29257914963704 33.0 19.0 36.0 2.0 39.0 49 25.960293011244637 32.0 17.0 36.0 2.0 39.0 50 25.561799751926635 32.0 7.0 36.0 2.0 39.0 51 24.742588299884098 32.0 2.0 36.0 2.0 39.0 52 24.48612212529738 32.0 2.0 36.0 2.0 39.0 53 24.160281827609346 32.0 2.0 36.0 2.0 39.0 54 23.83908782203786 32.0 2.0 36.0 2.0 39.0 55 23.49983224547063 32.0 2.0 36.0 2.0 39.0 56 13.310696638809247 17.0 2.0 22.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.4916564959559224 1 2 -2.244985024445022 1 3 -2.3128589838546105 1 4 -1.977608113099194 1 5 -1.6969713632453889 1 6 -2.707691090299086 1 7 -2.283277623859753 1 8 -2.442631446075211 1 9 -2.5330839297392345 1 10 -2.5270027561583532 1 11 -3.2909552760512426 1 12 -3.7087867189555155 1 13 -2.628617371044591 1 14 -2.771790885460888 1 15 -3.6835068689813966 1 16 -3.494347813292606 1 17 -3.5344004769091626 1 18 -3.5703776443593966 1 19 -3.173614026680646 1 20 -3.579841212838737 1 21 -3.003002923135689 1 22 -3.055563247549472 1 23 -3.251417151592605 1 24 -3.0762923228751333 1 25 -3.2621818773182767 1 26 -3.5290039986498023 1 27 -3.0290546125892988 1 28 -3.5265564511689753 1 29 -3.0373074221058936 1 30 -3.7364670971193057 1 31 -3.7016285332626317 1 32 -4.068133355688314 1 33 -3.3245916000652294 1 34 -2.9054905355828318 1 35 -4.216109996202885 1 36 -3.4759641939469184 1 37 -3.393270766843603 1 38 -3.340878962216472 1 39 -3.253574486120044 1 40 -3.4731751372637305 1 41 -2.736629182907315 1 42 -3.226955970882404 1 43 -2.411219678130138 1 44 -2.2552624025029893 1 45 -2.057879701250087 1 46 -2.4653008427163137 1 47 -2.9133757575006207 1 48 -2.7919292428890614 1 49 -3.0920576629578704 1 50 -2.6716909554514743 1 51 -3.1298558678101003 1 52 -2.9465657804449528 1 53 -3.01762088222349 1 54 -3.2281712035706036 1 55 -4.285325302690051 1 56 -1.9370019523854474 2 1 -0.26587465003677835 2 2 -0.3260300282589057 2 3 -0.3107613484617815 2 4 -0.4344158934195548 2 5 -0.048420638607709066 2 6 -0.4247963534569834 2 7 -0.39883826459202254 2 8 -0.19282214020183375 2 9 -0.47520826230979196 2 10 0.06662806001250487 2 11 -0.5285601578208841 2 12 -0.5797821422964802 2 13 -0.2812680194046209 2 14 -0.41366731566684223 2 15 -0.5094031313765086 2 16 -1.1530053266411997 2 17 -0.4976918575346403 2 18 -0.5175553712854111 2 19 -0.5368863378934634 2 20 -0.14248423343370575 2 21 -0.08452466226612643 2 22 -0.08731381963185214 2 23 0.19690474009313164 2 24 -0.17316493919854992 2 25 0.17221934312413367 2 26 0.4080669395653018 2 27 0.11911472379514265 2 28 -0.7700728508638619 2 29 -0.3044698935017749 2 30 -0.7556318568447082 2 31 -0.6830358559170939 2 32 0.19559662142838974 2 33 -0.10634217214760966 2 34 -0.9519245554150224 2 35 0.6822500343081757 2 36 0.34249499750998424 2 37 0.74517316908317 2 38 0.6866763390802468 2 39 0.4872721958021522 2 40 1.1489644508369565 2 41 1.8941869879546225 2 42 1.3971859055478042 2 43 2.2545888649667347 2 44 1.97328450825216 2 45 2.546906721328096 2 46 2.0097182266963465 2 47 2.1056936551614704 2 48 1.7640394832741748 2 49 1.5179728481023886 2 50 1.3370809743730874 2 51 1.1333020269267422 2 52 1.7015082088761773 2 53 1.2073028401258625 2 54 1.4461232281608964 2 55 1.0565892663412235 2 56 0.7185091078738974 3 1 -0.10703443572855065 3 2 -0.6056549888640106 3 3 -0.3054824326092742 3 4 0.13290998114710106 3 5 0.1416991218713335 3 6 -0.0011042639517810926 3 7 0.004407874708327597 3 8 0.1280098799683529 3 9 0.17398020286512406 3 10 -0.20889983157274372 3 11 -0.49940793594883814 3 12 -0.41979947605002366 3 13 -0.8586789869432252 3 14 0.1446371280772496 3 15 -0.5772411395706527 3 16 -0.5920220300764356 3 17 -0.578963524726575 3 18 -0.3925159763909889 3 19 -0.4731453987191827 3 20 -0.36518357159948067 3 21 -0.717009692206247 3 22 -1.060249527164153 3 23 -0.4818692907924671 3 24 -0.41804360292373133 3 25 -1.42563757461933 3 26 -1.4958095180457889 3 27 -1.0431134514333493 3 28 -1.0750681234765338 3 29 -0.937073108125162 3 30 -0.8735407758488023 3 31 -0.8226907566419612 3 32 -0.2800573338190091 3 33 -0.853229975488297 3 34 -0.6234893206214522 3 35 -0.2050017778569675 3 36 -0.009852938197550287 3 37 -0.18785551039997017 3 38 -0.024598479703236364 3 39 -0.033449518663651645 3 40 -0.060261833436602075 3 41 0.33336127096754353 3 42 0.6927264034992682 3 43 0.3098599018403547 3 44 0.8505694121286176 3 45 0.3821966677510389 3 46 1.6988925097092675 3 47 0.7470425363464699 3 48 1.1993924615281912 3 49 1.1868193655937205 3 50 0.45305954355052336 3 51 0.3102638926689423 3 52 1.0507439479243956 3 53 0.40345001945141945 3 54 0.3314052956049558 3 55 0.07274117305411565 3 56 0.7813439644764806 4 1 -0.001748075932802351 4 2 -0.27477074508163923 4 3 -0.11129412211369782 4 4 0.004920561213957342 4 5 0.15863457611622067 4 6 -0.4254825013420316 4 7 0.16670903925040648 4 8 0.03514040419597109 4 9 0.11193251909156743 4 10 -0.21114074851187326 4 11 -0.6682114332487217 4 12 -0.4383145930552814 4 13 -0.42225122896226 4 14 0.03402399850816806 4 15 -0.8585313199460956 4 16 -0.4943033569931501 4 17 -0.15425466024694146 4 18 -0.7966157522992958 4 19 -0.10412883062836897 4 20 -0.6517270490617335 4 21 -0.5944939874194972 4 22 -0.633850790894364 4 23 -0.9654297771816474 4 24 -1.0491739802059783 4 25 -1.543634709184552 4 26 -0.23545905333104855 4 27 -0.44489883771599636 4 28 -0.38122880824197125 4 29 -0.7433623700920222 4 30 -0.1268378626567852 4 31 -0.23990540709565522 4 32 -0.6333507469926616 4 33 -0.501038652612575 4 34 -0.014364012954576566 4 35 -0.2232230041161074 4 36 -0.7844019995839773 4 37 0.4437524393447134 4 38 0.07661014599015914 4 39 0.32671359070044304 4 40 0.6351204082150623 4 41 1.0057465617357018 4 42 0.5411155148471245 4 43 0.49284362764029055 4 44 1.2410032683425705 4 45 1.1196787587836994 4 46 1.8988851624406173 4 47 1.2848993380804252 4 48 1.1308057086535754 4 49 1.1783700066429112 4 50 0.7281045455710213 4 51 0.2658496536622934 4 52 0.951629293797069 4 53 1.3968007413205648 4 54 0.5791551945800144 4 55 0.5452072837774509 4 56 0.5796247036117066 5 1 -0.5938821688337654 5 2 -0.4284736372814635 5 3 -0.36151322816103004 5 4 -0.24080687086316743 5 5 -0.40556349575056316 5 6 -0.17792918553718096 5 7 -0.1807931518100645 5 8 -0.242320887068999 5 9 -0.23805287383861184 5 10 -0.6235348472556623 5 11 -0.5551892305025916 5 12 0.16802487524738297 5 13 0.2140327324750757 5 14 0.19974120563642117 5 15 0.004381329776371956 5 16 0.5866563274941399 5 17 0.8581978667761376 5 18 0.7947879871105812 5 19 -0.29033245318168355 5 20 0.3174155159397287 5 21 -0.013096090837557028 5 22 -0.001474220634356982 5 23 -0.07439851805223441 5 24 0.020757366565860025 5 25 -0.14701624584829887 5 26 0.40681380673321854 5 27 0.5974981824417611 5 28 -0.408544028808727 5 29 0.33588098369120445 5 30 0.25188694014777724 5 31 0.4942824398222534 5 32 1.1642683006263894 5 33 0.3842593316117906 5 34 1.0665591538581616 5 35 1.0553703350600543 5 36 1.0530213132994568 5 37 1.1515014898851739 5 38 0.11305478519553702 5 39 0.7541894890352374 5 40 0.1445784859246757 5 41 0.26699400549848207 5 42 0.7101558303598345 5 43 0.841368313588287 5 44 0.8868183428386267 5 45 0.7524205057892495 5 46 1.3468109585259214 5 47 1.0436635799735008 5 48 1.0954931173593856 5 49 0.8704164571249464 5 50 1.0544995207390038 5 51 0.6677631798089472 5 52 0.577761341708257 5 53 0.6327414366170885 5 54 0.27726357903809173 5 55 0.7185566848875915 5 56 0.6144990828112391 6 1 0.2290524266911902 6 2 0.02225786013678288 6 3 0.20303066168406758 6 4 0.16173185547074098 6 5 0.4666396023561461 6 6 0.4429113193394656 6 7 0.43280409431729794 6 8 0.29195908998206477 6 9 0.4825437985652883 6 10 -0.012160779556296575 6 11 -0.39476180652094683 6 12 0.166016843118868 6 13 -0.034676389728019785 6 14 0.4503149291017081 6 15 0.06906865048778599 6 16 0.016303487562986163 6 17 -0.9671750534763035 6 18 0.1829836268122449 6 19 0.4623210178452837 6 20 0.4801378337825284 6 21 -0.15229574660347822 6 22 0.06701401803460527 6 23 -0.9364180246500524 6 24 -0.28381807806982806 6 25 -0.1473748230141041 6 26 0.16298133398508696 6 27 0.043496461271359976 6 28 0.4672448409560488 6 29 0.09391311220526433 6 30 0.07180948747996041 6 31 1.0613363698279947 6 32 1.1015890120434833 6 33 1.2136483161212546 6 34 1.473329090748578 6 35 1.6158264452149602 6 36 1.5650695060705395 6 37 1.43255427244398 6 38 1.0891018305196916 6 39 2.211590521737474 6 40 0.8408636264869251 6 41 2.3402871781892465 6 42 2.5124363811343606 6 43 2.2358031959749773 6 44 2.1753295304461986 6 45 1.8629339882906848 6 46 2.223890706087598 6 47 1.145642925928751 6 48 2.2623749303255387 6 49 2.338359658501883 6 50 2.6817226991670005 6 51 2.9785778671296583 6 52 3.282437187950368 6 53 3.1232032840066495 6 54 3.8440306473111825 6 55 3.949265233367221 6 56 1.3598375796557605 7 1 0.3447824274395259 7 2 0.44031518259431124 7 3 0.0018408091060564402 7 4 0.7025161105032964 7 5 0.5804613489699335 7 6 0.23019710845867536 7 7 0.381318649432167 7 8 0.37827952700967415 7 9 0.3126717638425447 7 10 0.23179641568429332 7 11 0.5827507280895432 7 12 0.4713375046469679 7 13 0.6922936020402943 7 14 0.2468426549117808 7 15 0.45210389706415555 7 16 0.1899171970593585 7 17 0.6467589433806893 7 18 0.3292082769656517 7 19 1.077938768350407 7 20 1.0692271101426272 7 21 0.2733945923922576 7 22 0.8761137710840714 7 23 0.7434068649705452 7 24 1.1390285880979505 7 25 0.6870934850005597 7 26 1.1449090448284593 7 27 1.3933056358579066 7 28 1.3171288490380633 7 29 0.9999596586394475 7 30 0.6020422196508832 7 31 1.4784956903398516 7 32 1.9877672654296958 7 33 1.2996320024192407 7 34 2.11831898822669 7 35 1.978506981022786 7 36 1.8138908785168475 7 37 1.84275407787689 7 38 1.765746296582698 7 39 1.5072950790973465 7 40 1.5392472229847165 7 41 1.3762072560160803 7 42 1.4469570725958576 7 43 1.5970618953688245 7 44 1.3192717589876928 7 45 1.176074750095669 7 46 1.853539736993831 7 47 2.144595256992133 7 48 1.8002032622869244 7 49 1.5777145937709065 7 50 1.0974601832648823 7 51 1.6744919582768567 7 52 1.4964984017496654 7 53 1.8374515815446273 7 54 1.2418080924956492 7 55 1.1473869126308607 7 56 0.061962850927182345 8 1 -0.10115730110889842 8 2 0.14229355848573277 8 3 0.1455414455603119 8 4 0.5336640286077312 8 5 0.30651763299722745 8 6 0.18737063809597743 8 7 0.0449564072728279 8 8 -0.17397873539351139 8 9 0.06970726725486287 8 10 -0.08980468852550416 8 11 0.43180371482368685 8 12 -0.03567882845632653 8 13 -0.06903605059370221 8 14 0.6675542567828003 8 15 0.09829667369171347 8 16 0.9135963627675068 8 17 0.35180456871616883 8 18 0.9325745832305117 8 19 0.5110343898518295 8 20 0.8670186905429063 8 21 0.10220373279560135 8 22 0.22758221675541535 8 23 0.39297361598656977 8 24 1.399284703426531 8 25 0.7160789922469419 8 26 -0.33039959714685097 8 27 -6.058739956316117E-4 8 28 0.4133254597274316 8 29 -0.22276099161743446 8 30 0.01930316589733394 8 31 -0.213169059295911 8 32 -0.2135976605729084 8 33 0.21164028399274315 8 34 -0.1274443734786992 8 35 0.4140564020794564 8 36 -0.20623794595980272 8 37 0.16979954984990187 8 38 -0.21366479152403883 8 39 0.39484204635445863 8 40 -0.048322748643226276 8 41 0.5046483264861372 8 42 -0.11987062466662124 8 43 0.7082113470979863 8 44 0.8045873022742498 8 45 0.5674557791578572 8 46 1.401043762758725 8 47 1.4003655200087728 8 48 1.1023273325269365 8 49 1.5064393848145414 8 50 1.3406547235608741 8 51 -0.0619546332421983 8 52 0.9353451159587003 8 53 0.3688525477281992 8 54 0.686214196322041 8 55 -0.041363950033044716 8 56 -0.563410970098813 9 1 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27 0.8817993129659101 120 28 1.1887495648877433 120 29 1.4156171947223797 120 30 0.1911212669720328 120 31 1.989957328482756 120 32 2.323483785381441 120 33 2.5773477180454982 120 34 1.203934967492966 120 35 2.2209664296129823 120 36 2.826402608263482 120 37 2.255775213763215 120 38 2.3169773614202676 120 39 2.08846492582866 120 40 2.4464369991141233 120 41 2.442653477920544 120 42 2.6856183175129686 120 43 2.3658630035506043 120 44 2.2641307786042972 120 45 2.1193698034939423 120 46 2.937681119539967 120 47 3.4354929736014554 120 48 3.0320724253415747 120 49 3.175015634925561 120 50 3.415839020567713 120 51 3.3845326213985345 120 52 4.143622938145395 120 53 3.8236886788233235 120 54 4.401064538270706 120 55 4.2460061538838225 120 56 3.5715477296080884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99.0 3 25.0 4 55.0 5 67.0 6 108.0 7 265.0 8 179.0 9 379.0 10 484.0 11 512.0 12 778.0 13 1022.0 14 1169.0 15 1522.0 16 1805.0 17 1948.0 18 2157.0 19 2412.0 20 2694.0 21 2704.0 22 2942.0 23 3599.0 24 4042.0 25 4347.0 26 4005.0 27 4480.0 28 4944.0 29 5926.0 30 6791.0 31 7851.0 32 9333.0 33 12211.0 34 15416.0 35 19250.0 36 22388.0 37 24300.0 38 18296.0 39 6211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 57.515911262937436 22.656520059374937 3.1487016816120708 16.678866996075563 11 33.7044266861872 15.963114337420444 18.41588889566685 31.916570080725513 12 33.450761503893936 10.09068911527278 34.6265682506761 21.83198113015718 13 21.575265865511703 11.76975945017182 38.04723560869477 28.60773907562171 14 15.88584558449745 15.897537566847639 48.300087435694095 19.916529412960816 15 23.565444600337543 13.859065861444925 41.20762927265702 21.367860265560505 16 27.9580715345981 12.562780861749934 29.3072246283983 30.17192297525367 17 27.683564122897984 13.559141096809613 24.341690558978428 34.41560422131398 18 31.58563614550926 15.31903861404258 27.57274446410053 25.522580776347624 19 30.758555480997984 19.12960816608715 27.745074117001163 22.366762235913704 20 30.88360885743915 20.5499298481059 27.707456434657068 20.85900485979788 21 33.38518473332113 18.568901360336728 26.35982838203298 21.686085524309156 22 35.16694117407837 17.121637284206674 27.016612781878447 20.694808759836516 23 37.72240183818296 20.139185424673133 23.5212184062303 18.6171943309136 24 36.33969783850831 22.703287988775696 21.00286707741109 19.954147095304904 25 32.44837458112348 20.185398943337894 17.31593586803419 30.050290607504436 26 26.805560500709557 18.187598231952023 22.951053148254587 32.05578811908383 27 25.36449083238765 18.220724961451754 22.81903443643229 33.59574976972831 28 27.207158599838138 23.646691743483505 21.454421443223406 27.69172821345495 29 30.227336371273644 18.06776813217586 21.273898322344138 30.430997174206357 30 27.17534700114606 17.152680504265884 25.28664204762511 30.385330446962943 31 37.539036828350035 14.730830561060476 24.62542475635415 23.104707854235336 32 42.81964372180739 12.573205229491066 25.01694742475598 19.590203623945566 33 39.686594868029204 12.615244972055645 27.00148900583364 20.696671154081507 34 29.379334965320275 17.621889024887803 36.45297837617299 16.545797633618932 35 27.260485684993345 26.17812530285178 32.03419483507011 14.527194177084768 36 30.423466731988118 30.81029221143738 23.438153852434755 15.328087204139749 37 31.571666309330553 26.187632086740788 24.6847785026596 17.55592310126905 38 29.8093322031475 27.338412361237353 23.57714028942289 19.275115146192263 39 32.38889342755606 23.95122748253748 24.338262325885378 19.321616764021076 40 34.028107710463 19.917035765554516 22.878511548069703 23.176344975912784 41 29.61029141292353 22.036314219152327 26.168410512118363 22.18498385580578 42 26.509264182584154 22.13127326271013 26.64679536995378 24.712667184751933 43 27.125013421137396 15.999529621089337 25.301272579465916 31.57418437830735 44 26.10518351133174 15.90431541761034 26.306613974366183 31.683887096691738 45 30.242455242966752 17.464961636828644 28.485421994884913 23.80716112531969 46 28.938146808608593 19.224571437341094 29.82264079517493 22.01464095887538 47 29.47160503935074 18.851511088823163 28.218419625221316 23.458464246604784 48 28.71901989996263 15.360907606219316 29.234053663855995 26.686018829962066 49 25.439754412893322 16.27321565617805 29.43054489639294 28.85648503453569 50 20.64037265491029 15.871618335850119 32.90368815745694 30.584320851782653 51 18.59686485711653 17.532534018655227 37.19372971423306 26.67687140999519 52 17.960471393232304 18.21911283424925 37.85691238457166 25.963503387946794 53 17.513638109873224 19.043667563068254 37.58893584325778 25.853758483800743 54 19.06555369376848 16.75044708510758 36.2705159541488 27.913483266975142 55 19.633718398606593 17.674752183601854 27.8999001050178 34.79162931277375 56 20.69525566144072 19.65519008095092 25.08504969771493 34.564504559893436 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 9.0 9 18.0 10 72.0 11 126.0 12 164.0 13 235.5 14 269.0 15 512.5 16 756.0 17 957.5 18 1159.0 19 1203.5 20 1248.0 21 1246.5 22 1667.0 23 2089.0 24 2354.0 25 2619.0 26 2886.0 27 3153.0 28 3414.5 29 3521.5 30 3367.0 31 2844.0 32 2321.0 33 2896.5 34 3472.0 35 2872.5 36 2273.0 37 1940.0 38 1778.5 39 1950.0 40 2061.0 41 2172.0 42 2396.0 43 2620.0 44 3259.5 45 3899.0 46 3523.5 47 3221.5 48 3295.0 49 4338.5 50 5382.0 51 6304.0 52 7226.0 53 7628.0 54 8945.5 55 9861.0 56 13781.0 57 17701.0 58 18500.0 59 19299.0 60 21741.5 61 24184.0 62 23213.0 63 17798.0 64 13354.0 65 11110.0 66 8866.0 67 7181.5 68 5497.0 69 4232.0 70 2967.0 71 2550.0 72 1686.0 73 1239.0 74 1059.5 75 880.0 76 689.5 77 499.0 78 414.5 79 255.0 80 180.0 81 129.5 82 79.0 83 62.0 84 45.0 85 30.0 86 15.0 87 14.0 88 10.5 89 8.0 90 5.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.02999247646353118 26 0.05845991175095061 27 0.10624453526911894 28 0.12861180585209134 29 0.15809593525691862 30 0.19876369995323206 31 0.21706419406657312 32 0.26484881758474144 33 0.311108399926798 34 0.32330872933569205 35 0.3370340999206979 36 0.3873604587323858 37 0.4178612822546209 38 0.4463287175420403 39 0.44124524695500117 40 0.49258829988409686 41 0.49818011752984 42 0.5713820939832042 43 0.5739238292767238 44 0.5668069704548689 45 0.6181500233839646 46 0.6288253116167469 47 0.6578010939628703 48 0.7060940645397425 49 0.6435673763191606 50 0.6918603468960328 51 0.7025356351288151 52 0.7447284410012404 53 0.7579454645275423 54 0.7945464527542243 55 0.7681124057016206 56 0.7808210821692186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 196716.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.12277598161817 #Duplication Level Percentage of deduplicated Percentage of total 1 72.28408228191316 20.32829053051099 2 10.265355554752178 5.773805892759105 3 4.4466939011604785 3.751601293234917 4 2.7439355048624416 3.0866833404501923 5 1.8961715050070498 2.6662803229020517 6 1.391851343046166 2.3485634112121025 7 1.0194859188026464 2.00695418776307 8 0.936336358049239 2.1065902112690376 9 0.7031560681103358 1.7797230525224181 >10 3.683887061205307 18.769190101466073 >50 0.3362134413072557 6.614611927855385 >100 0.24944868226022196 13.68317777913337 >500 0.02169118976175843 4.046950934341894 >1k 0.02169118976175843 13.037577014579394 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 3357 1.706521076069054 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 3333 1.69432074666016 No Hit ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 3188 1.6206104231480918 No Hit ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 2713 1.3791455702637305 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2690 1.3674535879135403 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 2238 1.1376807173793693 No Hit ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1706 0.8672400821488848 No Hit ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 1516 0.7706541409951402 No Hit ATCAACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 1347 0.6847434880741781 No Hit ATCAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1325 0.6735598527826918 No Hit ATCAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1214 0.617133329266557 No Hit ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 1020 0.5185139998779966 No Hit ATCAACGGGGCCCAACTTCACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGA 989 0.5027552410581752 No Hit ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 843 0.4285365704874032 No Hit ATCAACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 839 0.42650318225258743 No Hit ATCAACGGGATCTCAGCCTACTAGAAACCAGTCTGGGATGGACATGATGGGCTTCT 782 0.39752739990646413 No Hit ATCAACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 607 0.3085666646332785 No Hit ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 605 0.3075499705158706 No Hit ATCAACGGGAAGTGGAGTATATTGAATATTGAAGTAGACTTCAGGTTGTTTTTTGG 604 0.3070416234571667 No Hit ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 581 0.29534964110697653 No Hit ATCAACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 574 0.2917912116960491 No Hit ATCAACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 523 0.2658655117021493 No Hit ATCAACGGGATTGTCCTCTCTGGTGGTTCAACCATGTTCAGGGACTTTGGACGTCG 507 0.25773195876288657 No Hit ATCAACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAG 507 0.25773195876288657 No Hit ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 445 0.22621444112324368 No Hit ATCAACGGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC 441 0.224181052888428 No Hit ATCAACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 435 0.22113097053620445 No Hit ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 423 0.21503080583175743 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 420 0.2135057646556457 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 406 0.20638890583379085 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC 381 0.1936802293661929 No Hit ATCAACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 378 0.19215518819008112 No Hit ATCAACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 365 0.1855466764269302 No Hit ATCAACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 359 0.1824965940747067 No Hit ATCAACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 357 0.18147989995729885 No Hit ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.180463205839891 No Hit ATCAACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 353 0.17944651172248316 No Hit ATCAACGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 352 0.17893816466377926 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 344 0.1748713881941479 No Hit ATCAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC 338 0.1718213058419244 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC 321 0.1631794058439578 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 320 0.16267105878525387 No Hit ATCAACGGGGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTGC 312 0.1586042823156225 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTTCC 307 0.15606254702210293 No Hit ATCAACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA 305 0.1550458529046951 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCG 298 0.15148742349376765 No Hit ATCAACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 297 0.15097907643506375 No Hit ATCAACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 288 0.14640395290672847 No Hit ATCAACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 287 0.14589560584802455 No Hit ATCAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 286 0.14538725878932066 No Hit ATCAACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 272 0.1382703999674658 No Hit ATCAACGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC 264 0.13420362349783446 No Hit ATCAACGGGGTGCATCAACGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCC 259 0.13166188820431485 No Hit ATCAACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.13115354114561092 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGCGTGGTTAAGAGCACTGACTGCTCTTC 256 0.1301368470282031 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTC 255 0.12962849996949916 No Hit ATCAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 255 0.12962849996949916 No Hit ATCAACGGGGGGCTAGAGAGATGGCTCAGCAGTTAAGAGCACTGACTTCTCTTCCA 239 0.12149494703023649 No Hit ATCAACGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA 238 0.12098659997153256 No Hit ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCAACTGCTCTTCCA 236 0.11996990585412472 No Hit ATCAACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 231 0.11742817056060513 No Hit ATCAACGGGGGGGCTGGAGAAATAGCTCAGCAGTTAAGAGCTCTGACTGCTCTTCC 228 0.11590312938449339 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTCCTCTTCC 226 0.11488643526708556 No Hit ATCAACGGGAACTTTAAACGATAAAAGTACTATCCAATAGGAGATTTATTCTTTCA 224 0.11386974114967771 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 223 0.11336139409097379 No Hit ATCAACGGGAGTCTTTTGTATTTTTGATTATTAAGTAAAACTTGCTTTTATTTTAA 221 0.11234469997356594 No Hit ATCAACGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT 219 0.11132800585615811 No Hit ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC 217 0.11031131173875029 No Hit ATCAACGGGATGGAGCTCGCGCTTCCCGACTTCCTCAAGGCCTTCTACGAGTGCCT 215 0.10929461762134245 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCTGACTGCTCTTCC 211 0.10726122938652677 No Hit ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCC 209 0.10624453526911894 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTGAGAGCACTGACTGCTCTTCCA 209 0.10624453526911894 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCC 206 0.10471949409300717 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC 203 0.10319445291689543 No Hit ATCAACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.1001443705646719 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.001525041176111755 0.0 0.0 0.0 0.0 11 0.001525041176111755 0.0 0.0 0.0 0.0 12 0.001525041176111755 0.0 0.0 0.0 0.0 13 0.001525041176111755 0.0 0.0 0.0 0.0 14 0.0025417352935195917 0.0 0.0 0.0 0.0 15 0.00305008235222351 0.0 0.0 0.0 0.0 16 0.00305008235222351 0.0 0.0 0.0 0.0 17 0.00305008235222351 0.0 0.0 0.0 0.0 18 0.00305008235222351 0.0 0.0 0.0 0.0 19 0.0035584294109274285 0.0 0.0 0.0 0.0 20 0.005591817645743102 0.0 0.0 0.0 0.0 21 0.006608511763150938 0.0 0.0 0.0 0.0 22 0.007116858821854857 0.0 0.0 0.0 0.0 23 0.010166941174078367 0.0 0.0 0.0 0.0 24 0.012708676467597959 0.0 0.0 0.0 0.0 25 0.012708676467597959 0.0 0.0 0.0 0.0 26 0.013217023526301877 0.0 0.0 0.0 0.0 27 0.013725370585005794 0.0 0.0 0.0 0.0 28 0.0305008235222351 0.0 0.0 0.0 0.0 29 0.03151751763964294 0.0 0.0 0.0 0.0 30 0.03710933528538604 0.0 0.0 0.0 0.0 31 0.03761768234408996 0.0 0.0 0.0 0.0 32 0.03761768234408996 0.0 0.0 0.0 0.0 33 0.03812602940279387 0.0 0.0 0.0 0.0 34 0.03914272352020171 0.0 0.0 0.0 0.0 35 0.05490148234002318 0.0 0.0 0.0 0.0 36 0.056934870574838856 0.0 0.0 0.0 0.0 37 0.05744321763354277 0.0 0.0 0.0 0.0 38 0.05896825880965453 0.0 0.0 0.0 0.0 39 0.06252668822058195 0.0 0.0 0.0 0.0 40 0.06456007645539763 0.0 0.0 0.0 0.0 41 0.06456007645539763 0.0 0.0 0.0 0.0 42 0.06710181174891722 0.0 0.0 0.0 0.0 43 0.06862685292502897 0.0 0.0 0.0 0.0 44 0.06862685292502897 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATCGG 35 5.0808012E-8 50.21961 50 TTAGAAG 15 0.008275993 50.21961 50 TGAAACT 15 0.008275993 50.21961 50 GCACCTC 15 0.008275993 50.21961 47 TTATATG 15 0.008275993 50.21961 49 TCGCAAA 20 4.0859956E-4 50.219604 50 GACGTCG 80 0.0 50.219604 50 TGCGGGC 20 4.0859956E-4 50.219604 48 CACAGGC 20 4.0859956E-4 50.219604 50 GTTTTAG 25 2.027807E-5 50.219604 47 CCTATCG 40 2.561137E-9 50.219604 49 CATTTGA 30 1.0141066E-6 50.20676 46 TAGATTA 20 4.0911607E-4 50.206753 46 GAATGTC 20 4.096331E-4 50.193913 43 GCAACTT 15 0.008292756 50.193913 44 CGCATTT 15 0.008292756 50.193913 43 TCTACGA 20 4.096331E-4 50.193913 44 AATGTCA 20 4.096331E-4 50.193913 44 AGATTAG 20 4.1015062E-4 50.18107 42 CAACGAC 15 0.008309544 50.168247 41 >>END_MODULE