FastQCFastQC Report
Tue 31 May 2016
SRR212603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186146
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC34221.8383419466440323No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT33351.7916044395259636No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC31081.669657150838589No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC27891.498286291405671No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC25421.3655947482083957No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC19941.0712021746371128No Hit
ATCAACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC18450.9911574785383517No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14770.7934631955561763No Hit
ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG14430.775197962889345No Hit
ATCAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA13240.7112696485554351No Hit
ATCAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11230.6032898907309316No Hit
ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG11040.5930828489465259No Hit
ATCAACGGGGCCCAACTTCACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGA9640.51787306737722No Hit
ATCAACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA8680.46630064572969604No Hit
ATCAACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT8450.45394475304331006No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC7490.40237233139578615No Hit
ATCAACGGGATCTCAGCCTACTAGAAACCAGTCTGGGATGGACATGATGGGCTTCT6410.3443533570423216No Hit
ATCAACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT5510.29600421174776786No Hit
ATCAACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA5480.29439257357128273No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC5320.2857971699633621No Hit
ATCAACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5280.28364831906138194No Hit
ATCAACGGGATTGTCCTCTCTGGTGGTTCAACCATGTTCAGGGACTTTGGACGTCG5090.27344127727697615No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC4980.2675319372965307No Hit
ATCAACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAG4860.2610853845905902No Hit
ATCAACGGGAAGTGGAGTATATTGAATATTGAAGTAGACTTCAGGTTGTTTTTTGG4840.2600109591396001No Hit
ATCAACGGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC4650.24980391735519433No Hit
ATCAACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC4430.2379852373943034No Hit
ATCAACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA4150.22294328108044223No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4090.219720004727472No Hit
ATCAACGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT4010.21542230292351164No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3990.21434787747252157No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC3920.2105873883940563No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC3730.20038034660965048No Hit
ATCAACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3620.19447100662920502No Hit
ATCAACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3520.18909887937425462No Hit
ATCAACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG3460.18587560302128436No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT3450.18533839029578933No Hit
ATCAACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3430.18426396484479923No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.1772801994133637No Hit
ATCAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC3140.16868479580544304No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG3010.16170103037400752No Hit
ATCAACGGGGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTGC2990.16062660492301742No Hit
ATCAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2950.15847775402103725No Hit
ATCAACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC2910.1563289031190571No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTTCC2830.15203120131509676No Hit
ATCAACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2830.15203120131509676No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC2740.14719628678564137No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCG2730.14665907406014633No Hit
ATCAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2580.13860088317772074No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC2550.13698924500123558No Hit
ATCAACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC2530.1359148195502455No Hit
ATCAACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA2440.13107990502079012No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC2430.1305426922952951No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2400.12893105411880995No Hit
ATCAACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.12678220321682981No Hit
ATCAACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG2340.12570777776583972No Hit
ATCAACGGGGGGGCTGGAGAAATAGCTCAGCAGTTAAGAGCTCTGACTGCTCTTCC2320.12463335231484964No Hit
ATCAACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC2280.12248450141286947No Hit
ATCAACGGGGTGCATCAACGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCC2250.12087286323638434No Hit
ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCAACTGCTCTTCCA2230.11979843778539426No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTC2180.11711237415791904No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTCCTCTTCC2160.11603794870692896No Hit
ATCAACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2150.11550073598143393No Hit
ATCAACGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA2140.11496352325593888No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGTGGTTAAGAGCACTGACTGCTCTTC2090.1122774596284637No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCC2080.11174024690296863No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC2080.11174024690296863No Hit
ATCAACGGGGGGCTAGAGAGATGGCTCAGCAGTTAAGAGCACTGACTTCTCTTCCA2050.1101286087264835No Hit
ATCAACGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2020.1085169705499984No Hit
ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCG2020.1085169705499984No Hit
ATCAACGGGAGTCTTTTGTATTTTTGATTATTAAGTAAAACTTGCTTTTATTTTAA2000.10744254509900832No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1980.10636811964801823No Hit
ATCAACGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA1980.10636811964801823No Hit
ATCAACGGGAACTTTAAACGATAAAAGTACTATCCAATAGGAGATTTATTCTTTCA1950.1047564814715331No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCC1940.10421926874603807No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC1940.10421926874603807No Hit
ATCAACGGGATGGAGCTCGCGCTTCCCGACTTCCTCAAGGCCTTCTACGAGTGCCT1940.10421926874603807No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCTGACTGCTCTTCC1900.10207041784405788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCG700.050.28912750
GGACGTC700.050.28912749
TTCTAGA150.0082301450.28912450
CGAGTTT252.0108366E-550.28912450
CTATCGG150.0082301450.28912450
CCGAGTT252.0108366E-550.28912449
ATCCCCT150.0082301450.28912449
CTTCTAG150.0082301450.28912449
ACGCACA800.050.27549447
CGCACAG850.050.2754948
TGAAGGG150.0082389750.27548248
ACTAATT150.0082389750.27548248
CATCCCC150.0082389750.27548248
ATTTATT252.0173014E-550.26186444
TTTGGAC700.050.26186446
GTTAATG204.068479E-450.2618646
TCGGGAC150.00824780750.26185645
TACGAGT150.00824780750.26185646
TCATCTC150.00824780750.26185645
TTCTACG150.008256650550.24823843
Produced by FastQC (version 0.11.5)