##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212603_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 186146 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.264394614979636 39.0 38.0 40.0 35.0 40.0 2 38.21854350885864 39.0 38.0 40.0 35.0 40.0 3 38.11533957216378 39.0 38.0 40.0 35.0 40.0 4 38.20609091788166 39.0 38.0 40.0 35.0 40.0 5 38.3413503379068 39.0 38.0 40.0 35.0 40.0 6 38.03135710678715 39.0 38.0 40.0 35.0 40.0 7 38.37368517185435 39.0 38.0 40.0 36.0 40.0 8 38.34771630870392 39.0 38.0 40.0 36.0 40.0 9 38.31414588548773 39.0 38.0 40.0 36.0 40.0 10 37.29185692950695 39.0 37.0 39.0 33.0 40.0 11 37.289863870295356 39.0 37.0 40.0 33.0 40.0 12 37.09558626024733 39.0 36.0 40.0 32.0 40.0 13 37.148179386073295 39.0 36.0 40.0 33.0 40.0 14 37.1883306651768 39.0 37.0 40.0 33.0 40.0 15 37.251625068494626 39.0 37.0 40.0 33.0 40.0 16 37.01200133228756 39.0 36.0 40.0 33.0 40.0 17 36.69078035520506 38.0 36.0 39.0 32.0 40.0 18 36.45157564492388 38.0 35.0 39.0 31.0 40.0 19 36.3494353894255 38.0 35.0 39.0 31.0 40.0 20 36.23549257034801 38.0 35.0 39.0 31.0 40.0 21 35.92853996325465 38.0 35.0 39.0 30.0 40.0 22 35.83259377048123 38.0 35.0 39.0 30.0 40.0 23 35.51055085792872 38.0 35.0 39.0 29.0 40.0 24 35.14412342999581 38.0 34.0 39.0 28.0 40.0 25 35.12992489766098 38.0 34.0 39.0 29.0 40.0 26 34.409968519334285 38.0 33.0 39.0 27.0 40.0 27 34.08737227767451 37.0 33.0 39.0 26.0 40.0 28 33.6625605707348 36.0 33.0 39.0 25.0 40.0 29 33.66240477904441 37.0 33.0 39.0 24.0 40.0 30 33.33493064583714 36.0 33.0 38.0 23.0 40.0 31 32.04515272957786 36.0 31.0 38.0 18.0 39.0 32 32.13086501993059 36.0 31.0 38.0 18.0 39.0 33 31.420137956227908 35.0 30.0 38.0 15.0 39.0 34 31.26099943055451 35.0 30.0 38.0 14.0 40.0 35 31.00383032673278 35.0 29.0 38.0 12.0 39.0 36 30.840001933965812 35.0 30.0 38.0 2.0 40.0 37 30.507402791357322 35.0 29.0 38.0 2.0 40.0 38 30.280870929270574 35.0 29.0 38.0 2.0 39.0 39 30.08233322230937 35.0 29.0 38.0 2.0 39.0 40 30.049740526253586 35.0 29.0 38.0 2.0 39.0 41 29.0660879094904 34.0 27.0 38.0 2.0 39.0 42 28.581092260913476 34.0 25.0 38.0 2.0 39.0 43 28.708460026001095 34.0 27.0 38.0 2.0 39.0 44 28.34570713311057 34.0 26.0 38.0 2.0 39.0 45 27.595371375157136 33.0 23.0 38.0 2.0 39.0 46 26.673600292243723 33.0 19.0 37.0 2.0 39.0 47 26.694809450646268 33.0 21.0 37.0 2.0 39.0 48 26.576805303364026 33.0 21.0 37.0 2.0 39.0 49 26.245516959805745 33.0 18.0 36.0 2.0 39.0 50 25.7680960106583 32.0 14.0 36.0 2.0 39.0 51 24.995922555413493 32.0 2.0 36.0 2.0 39.0 52 24.81309832067302 32.0 2.0 36.0 2.0 39.0 53 24.44115371804927 32.0 2.0 36.0 2.0 39.0 54 24.14397301043267 32.0 2.0 36.0 2.0 39.0 55 23.760875871627647 32.0 2.0 36.0 2.0 39.0 56 13.49849043224136 17.0 2.0 22.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.18319943086707724 1 2 -0.7313482505276809 1 3 -0.49827176670205375 1 4 -0.4282559128607488 1 5 -0.6132307730894198 1 6 -0.6491627504976094 1 7 -0.5228565564461931 1 8 -0.7014689510741263 1 9 -0.9767979658576635 1 10 -0.6624372616176686 1 11 -0.6898743624951962 1 12 -0.5139873041332947 1 13 -0.584583181785014 1 14 -0.4377981179594528 1 15 -1.0579195189754316 1 16 -0.6042456682234985 1 17 -1.0140318232231849 1 18 -0.7771412326328431 1 19 -0.31196515131065183 1 20 -0.6443916015647844 1 21 -0.6181642642008356 1 22 -0.761072960000341 1 23 -0.775959022869479 1 24 -0.6365853765595375 1 25 -0.8236177684882691 1 26 -1.2508833321360342 1 27 -1.7147860739771588 1 28 -1.3149379495927676 1 29 -1.84791724450875 1 30 -1.4545363738790869 1 31 -0.7959294510279413 1 32 -0.8875922209183926 1 33 -1.2865361274236413 1 34 -1.0613091773593553 1 35 -0.6620368388043083 1 36 -0.5953237409889418 1 37 -1.052075222570629 1 38 -0.8723734642860279 1 39 -1.0198692828093598 1 40 -0.5764471073893525 1 41 -0.18513147831071564 1 42 0.3563428091857581 1 43 0.6142745412363304 1 44 0.4710940492329918 1 45 0.12641054623436432 1 46 1.7087867093546762 1 47 1.642043463557112 1 48 0.8767599421255419 1 49 0.7276925759818802 1 50 1.2620873357185047 1 51 0.4747435876519326 1 52 1.019254444602268 1 53 1.0103347084285126 1 54 1.187436725456081 1 55 1.2526513784023834 1 56 0.4781086921561144 2 1 -0.33257831285465045 2 2 -0.386550113881718 2 3 -0.1790171083169625 2 4 -0.7124562234197569 2 5 -0.4871438165676807 2 6 -0.6700478436653086 2 7 -0.3230894757008471 2 8 -0.36994721194368907 2 9 -0.2954703261061127 2 10 -0.7408922305617693 2 11 -1.5505886482094837 2 12 -1.4490804718351527 2 13 -1.1095443619092364 2 14 -0.6721537080215683 2 15 -0.5867238668015204 2 16 -0.4012565377887185 2 17 -0.7380613263287756 2 18 -0.594454897229113 2 19 -0.7215536606274213 2 20 -0.510734769266648 2 21 -0.5690571213436897 2 22 -0.039605568695989746 2 23 -0.3750661657266221 2 24 -0.4271133268700922 2 25 -0.1718708740783299 2 26 -0.09521563027268343 2 27 0.25307106887999 2 28 0.09441764046934509 2 29 -0.07540171655843864 2 30 -0.4087289204629343 2 31 0.16657054897205725 2 32 0.3484714436778802 2 33 0.4221207048744944 2 34 0.18104330711269512 2 35 0.057761297841654624 2 36 0.09384551366944294 2 37 0.10130210662191885 2 38 0.5360116288816741 2 39 0.2972502824080294 2 40 -0.13770487136450882 2 41 0.33214336640978104 2 42 0.16480554210501452 2 43 -0.4616571357823034 2 44 -0.3285565718850201 2 45 0.7751683102095193 2 46 0.7441516161869757 2 47 0.7399083703894114 2 48 0.7501295073429333 2 49 -0.017066740788305168 2 50 1.8025376462775107 2 51 1.726995140446963 2 52 1.573602270689225 2 53 1.6413129692980775 2 54 1.6886013217293723 2 55 1.3454697013837524 2 56 1.5032251517834432 3 1 -0.46776733210164423 3 2 -0.527490225836317 3 3 -0.7711112728748972 3 4 -0.4622836906385288 3 5 -0.535452995311644 3 6 -0.8635146023494613 3 7 -0.18751705027334964 3 8 -0.2832590745309176 3 9 -0.3447763609193899 3 10 -1.139366273963347 3 11 -1.4438867081742117 3 12 -1.563987304133292 3 13 -1.1218517003035302 3 14 -0.872751821663158 3 15 -1.0843084078643201 3 16 -1.0217611003222657 3 17 -1.2926583664330593 3 18 -0.6485918499167909 3 19 -1.3297892253847294 3 20 -0.7737897497129325 3 21 -0.2551241407440443 3 22 -0.8270451822225624 3 23 -0.13344359077071033 3 24 0.471130672823179 3 25 -0.3306393734265427 3 26 -0.7809759247286294 3 27 -0.4638601480512321 3 28 -0.7173299249014065 3 29 -0.6889666272248007 3 30 -1.3696598306692103 3 31 -0.7408368584353511 3 32 -0.39986073943691025 3 33 -1.6495762508804326 3 34 -1.0857690539025633 3 35 -1.3733022709030749 3 36 -0.8674688027173367 3 37 -0.7595597904718616 3 38 -0.7396574149033128 3 39 -0.4821378013278803 3 40 -1.0809224160313278 3 41 -1.9038814783107156 3 42 -1.8885645982216488 3 43 -1.7094908908624369 3 44 -2.0010510124954024 3 45 -2.003836367345883 3 46 -2.5665990931144584 3 47 -2.2868917216280735 3 48 -2.140369687504087 3 49 -1.7773228561168857 3 50 -1.608128713664211 3 51 -1.3139909802493008 3 52 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0.5454500685348691 4 37 0.19524620600079956 4 38 0.5085789166663517 4 39 0.4292378600477811 4 40 0.40033860689636214 4 41 0.8401661407369048 4 42 -0.15972861938566751 4 43 1.3535602555220443 4 44 0.5606773825663254 4 45 0.8323629271867432 4 46 1.2299176617356302 4 47 1.3982934635571134 4 48 0.8404504183160171 4 49 1.6488235283628327 4 50 1.649587335718504 4 51 0.7836721590805062 4 52 1.1918734922213154 4 53 0.663310898904701 4 54 0.9166033921227488 4 55 1.2202109022119103 4 56 0.7420967873942086 5 1 -0.5723807174167845 5 2 -0.5346377242118905 5 3 -0.4842366789827537 5 4 -0.43596790116484385 5 5 -0.5588448081771418 5 6 -0.35669199026369114 5 7 -0.18491795995496574 5 8 -0.2855332785595053 5 9 -0.20702457404479446 5 10 -0.510061530623517 5 11 -0.4578568186355483 5 12 -0.30375338600463664 5 13 -0.36649107652185364 5 14 -0.5140773577255402 5 15 -0.5989282909052562 5 16 -0.11597812436384913 5 17 -0.3722555074337137 5 18 0.25524180830283427 5 19 -0.2147794787960322 5 20 -0.2952322448396387 5 21 -0.41951660338212093 5 22 -0.862132901520809 5 23 -0.7351329994776634 5 24 -0.8531058443957917 5 25 0.04330474613161073 5 26 -0.3053423964635229 5 27 -0.3517743780707221 5 28 -0.4964072478383841 5 29 -0.32087046088302174 5 30 -0.5590685376217763 5 31 -0.049803720033789745 5 32 0.06602620013423888 5 33 -0.262961566020131 5 34 -0.3466527446108181 5 35 0.7693228103184993 5 36 0.027592925677726754 5 37 0.6416747774293725 5 38 0.11154466436894239 5 39 0.38140995695671975 5 40 0.1487721908562598 5 41 0.04348694274191445 5 42 -0.3817419861358786 5 43 0.03839734825387353 5 44 -0.22002437181963685 5 45 -0.05516840113405763 5 46 0.4551682883020476 5 47 0.15852738168576863 5 48 0.6588505854003976 5 49 0.38869403797018265 5 50 0.6780961076483294 5 51 1.379641248470648 5 52 1.269254444602268 5 53 0.9253200885454689 5 54 1.1907261991402898 5 55 0.9332434836655423 5 56 0.18267740560640533 6 1 -0.5026605087113865 6 2 -1.0092674122043235 6 3 -0.5892897307739133 6 4 -0.4599550146571545 6 5 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0.17029841015241942 8 13 0.34238110392927723 8 14 0.3052375963262577 8 15 -0.0697052332611463 8 16 -0.06585281108064578 8 17 0.05721817677681429 8 18 0.21250162451001842 8 19 0.4985705629750612 8 20 0.15435839843521393 8 21 0.5966571643705905 8 22 0.8523198971425145 8 23 0.6420766914162357 8 24 1.214128909154752 8 25 0.4904893743688703 8 26 0.32590238214967826 8 27 0.3330710688799883 8 28 0.6198834789786645 8 29 0.8642256126341081 8 30 0.42903505469234204 8 31 0.04657054897205626 8 32 0.061514921938751854 8 33 0.5729281582906438 8 34 1.2202979654977888 8 35 0.7054631611956914 8 36 0.8180691161539144 8 37 0.31024620600080155 8 38 0.4661979642853993 8 39 0.510666431476352 8 40 0.6953386068963603 8 41 1.0446899502607145 8 42 1.335985666328618 8 43 1.0864173983791865 8 44 1.4987726206615655 8 45 1.176410546234365 8 46 1.8139652807832505 8 47 2.255436320699971 8 48 1.872117084982687 8 49 1.9757282902675932 8 50 1.4974444785756482 8 51 1.2301007305090756 8 52 1.2178258731736982 8 53 1.380691851285654 8 54 1.1720795825989363 8 55 1.1335442355452408 8 56 1.1390015492989711 9 1 -0.19593235012172983 9 2 -0.13810290891277077 9 3 -0.11690530707472391 9 4 -0.08512703087317419 9 5 0.12155183560622618 9 6 -0.09240809211251388 9 7 0.0992707727463582 9 8 0.01514595575817168 9 9 -0.15882436337319206 9 10 -0.87753819329469 9 11 -0.25245200224674846 9 12 -0.24411152773577527 9 13 -0.6437058525924613 9 14 -0.18147047820790618 9 15 -0.18299715872698386 9 16 -0.24597703468312915 9 17 0.12529271093830374 9 18 0.18815379842305902 9 19 0.2163345381303401 9 20 -0.05110744007410517 9 21 0.5365329407681116 9 22 0.32685405863320227 9 23 0.674623275267173 9 24 0.2995947476640666 9 25 -0.1159702529603237 9 26 0.31472225792607844 9 27 -0.7593512913684606 9 28 -0.46458857071078086 9 29 0.06745542629870016 9 30 0.3055567938227739 9 31 0.6324090582888253 9 32 0.2304590213176354 9 33 0.2606300216446833 9 34 0.8704843009015164 9 35 -0.20310826737573606 9 36 1.4727275012470855 9 37 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119 31 1.699504680708582 119 32 1.4120709527343038 119 33 1.6874533935344438 119 34 2.071213277730468 119 35 1.7284571731717406 119 36 1.1872735644002788 119 37 1.3063454361120073 119 38 0.9075666554744508 119 39 1.1441651483283621 119 40 1.2706337309340014 119 41 1.599112533665334 119 42 1.5368068810420468 119 43 1.586879331655492 119 44 1.6794398576162273 119 45 1.7021590492283742 119 46 1.9893630566600677 119 47 1.7515868767307659 119 48 1.5431521576944043 119 49 2.4501850909519405 119 50 2.3524316470957487 119 51 2.2189801145980397 119 52 2.335122708075321 119 53 1.825379618608153 119 54 2.216254090725542 119 55 1.7053834143305302 119 56 1.3182658777848566 120 1 -0.6144494308670758 120 2 -0.45009825052768093 120 3 -0.30452176670205233 120 4 -0.09700591286075166 120 5 -0.600730773089424 120 6 -0.04916275049760799 120 7 -1.0728565564461903 120 8 -0.7264689510741249 120 9 -1.1267979658576621 120 10 0.3875627383823286 120 11 -0.3773743624951962 120 12 -0.07023730413329332 120 13 0.12166681821499026 120 14 -0.09404811795945278 120 15 -0.07666951897543584 120 16 -0.04799566822349988 120 17 -0.07028182322318344 120 18 0.11035876736715977 120 19 0.3817848486893496 120 20 0.12435839843521279 120 21 0.6068357357991658 120 22 0.551427039999659 120 23 0.661540977130521 120 24 0.8821646234404668 120 25 0.3576322315117295 120 26 0.6303666678639672 120 27 0.5664639260228412 120 28 0.803812050407231 120 29 0.8020827554912486 120 30 1.0329636261209103 120 31 1.9478205489720573 120 32 1.9999077790816102 120 33 2.075963872576356 120 34 2.3511908226406426 120 35 2.6879631611956896 120 36 2.6484262590110603 120 37 2.5666747774293697 120 38 2.2401265357139692 120 39 2.5051307171906387 120 40 1.9360528926106468 120 41 2.708618521689285 120 42 3.168842809185758 120 43 2.5892745412363283 120 44 3.408594049232992 120 45 2.788910546234362 120 46 3.721286709354679 120 47 3.454543463557112 120 48 3.995509942125544 120 49 3.715192575981881 120 50 3.8870873357185047 120 51 4.262243587651934 120 52 3.7880044446022687 120 53 4.479084708428513 120 54 4.693686725456082 120 55 4.477651378402385 120 56 3.6656086921561144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65.0 3 13.0 4 39.0 5 52.0 6 80.0 7 210.0 8 145.0 9 316.0 10 407.0 11 401.0 12 680.0 13 1006.0 14 1237.0 15 1244.0 16 1527.0 17 1708.0 18 1880.0 19 2019.0 20 2369.0 21 2399.0 22 2573.0 23 3073.0 24 3697.0 25 4024.0 26 3627.0 27 4016.0 28 4440.0 29 5093.0 30 5838.0 31 6692.0 32 8450.0 33 11060.0 34 14580.0 35 18369.0 36 22954.0 37 24210.0 38 19325.0 39 6328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 58.77375823278502 21.21238167889721 3.116371020596736 16.897489067721036 11 33.107345846808414 15.267048445843583 18.358170468342056 33.26743523900594 12 32.398225049154966 9.95777507977609 35.32872046673042 22.315279404338533 13 21.216142167975676 11.652681228712945 38.1039614066378 29.027215196673577 14 15.635576375533184 15.482470748767096 49.12111998109011 19.760832894609607 15 22.93844616591278 13.45180664639584 41.9933815392219 21.616365648469483 16 27.18242669732361 12.17109150881566 29.444629484383228 31.201852309477506 17 27.07981906675405 13.084890354882727 24.116553672923406 35.71873690543982 18 31.239457200262162 14.997367657645075 27.691167148367413 26.072007993725354 19 31.41834903785201 18.214197457909385 27.64765291760231 22.719800586636296 20 30.522815424451778 19.67863934760887 28.53620276557111 21.26234246236825 21 33.22821871004481 17.81827167921954 26.48512457963104 22.468385031104617 22 35.192835615628844 16.73928109034452 26.96851345470955 21.099369839317085 23 38.099689477699336 19.429670459551517 23.57175858771449 18.898881475034653 24 35.60323616945838 22.67897241949867 21.761950297078634 19.955841113964308 25 32.60159281192567 20.600152617606913 17.203336091914494 29.594918478552927 26 27.154419079884917 17.7720416229733 22.839934392729425 32.23360490441235 27 25.674658234110982 18.054166263323758 22.34519699267529 33.92597850988997 28 28.04811137050704 23.24235349807963 20.891652591150176 27.81788254026315 29 30.309177392277558 17.913563772545306 20.9617536911885 30.815505143988638 30 27.48752133622662 17.103442334304344 24.836713924950327 30.572322404518705 31 37.084291538531964 15.069179273684947 23.98035297855954 23.866176209223543 32 42.797121434111865 12.416508661553047 23.998103938636557 20.78826596569853 33 39.94104999973057 12.908110205247306 26.040381289032823 21.1104585059893 34 29.79115479115479 17.73460062933747 35.81619897409371 16.658045605414028 35 28.064189225611003 25.39970029216124 31.431590068674065 15.104520413553693 36 31.721236312638222 29.898052753654458 22.79033389071687 15.590377042990452 37 31.560528444324614 25.69479644108924 24.175249393367483 18.569425721218657 38 30.12222432075115 26.821897849607424 23.412567789979224 19.643310039662197 39 32.320713368094914 23.611425980503288 24.42055035571245 19.647310295689348 40 34.811702374715786 19.712247311769886 22.20686005001107 23.26919026350326 41 30.815439051400478 21.40291170353564 25.893633687167437 21.88801555789644 42 27.202870513471744 21.78391172306462 26.64087239400391 24.372345369459726 43 27.973755181676186 15.786344695639015 24.398060823555483 31.841839299129315 44 26.470270328696348 15.817093074786257 25.822281310461857 31.890355286055538 45 30.233639302433694 17.417534299892964 28.057258387121188 24.29156801055215 46 28.902921649618303 19.00261672541684 29.10620445059579 22.988257174369068 47 29.808009607634173 18.364862891053974 27.593817790352333 24.23330971095952 48 28.441156414724695 15.472621199493448 28.49148708179545 27.594735303986408 49 25.4913838797962 16.841729498177255 28.441850653916468 29.22503596811008 50 21.4867892668882 16.362150519012413 31.852425124746986 30.2986350893524 51 19.087574655933523 18.09378516402666 36.2108326841513 26.607807495888515 52 18.71156677637277 18.949203187250994 36.41953923436688 25.919690802009356 53 17.96615307989649 19.505289257988025 36.4303734421863 26.098184219929188 54 19.75399540906925 17.188921131274633 35.19370696002425 27.86337649963186 55 19.714121674321866 18.378975647803102 26.893036658289283 35.013866019585755 56 20.75945802510587 19.767353702520335 24.492304693000033 34.98088357937376 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 7.5 9 15.0 10 58.5 11 102.0 12 138.0 13 208.5 14 243.0 15 489.0 16 735.0 17 899.5 18 1064.0 19 1075.5 20 1087.0 21 1097.0 22 1475.5 23 1844.0 24 2109.0 25 2374.0 26 2633.0 27 2892.0 28 3014.0 29 3120.0 30 3104.0 31 2578.0 32 2052.0 33 2533.0 34 3014.0 35 2448.5 36 1883.0 37 1635.5 38 1539.5 39 1691.0 40 1817.0 41 1943.0 42 2169.5 43 2396.0 44 2990.0 45 3584.0 46 3307.5 47 3035.0 48 3039.0 49 3994.0 50 4949.0 51 5775.5 52 6602.0 53 6925.0 54 8148.5 55 9049.0 56 12955.5 57 16862.0 58 17842.0 59 18822.0 60 20966.5 61 23111.0 62 22450.0 63 17545.5 64 13302.0 65 10971.5 66 8641.0 67 7012.0 68 5383.0 69 4196.5 70 3010.0 71 2548.0 72 1717.5 73 1349.0 74 1094.5 75 840.0 76 683.5 77 527.0 78 427.5 79 253.0 80 178.0 81 125.5 82 73.0 83 58.5 84 44.0 85 37.5 86 31.0 87 24.5 88 11.5 89 5.0 90 3.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0016116381764851243 23 0.0042977018039603325 24 0.0 25 0.03223276352970249 26 0.10260763056955294 27 0.10744254509900832 28 0.13107990502079012 29 0.16008939219752238 30 0.23046425923737282 31 0.2519527682571745 32 0.2675319372965307 33 0.30352518990469846 34 0.297615849924253 35 0.3395184425128663 36 0.4061328204742514 37 0.3739000569445489 38 0.4464237748863795 39 0.4748960493376167 40 0.530228960063606 41 0.5538663199853878 42 0.5866362962405853 43 0.6011410398289515 44 0.5973805507504861 45 0.6231667615742482 46 0.634985441535139 47 0.6946160540650886 48 0.7349070084772168 49 0.6768880341237523 50 0.7370558593791969 51 0.6973021176925639 52 0.7574699429480086 53 0.7692886229088994 54 0.7703630483598896 55 0.8176377682034531 56 0.7993725355366218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 186146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.67773683023003 #Duplication Level Percentage of deduplicated Percentage of total 1 71.60769395003979 19.81938908168857 2 10.453989635294345 5.786855479032587 3 4.716523359406844 3.9162807688588526 4 2.899788435783467 3.2103832475583682 5 1.9623066322470448 2.715610327377435 6 1.3936064905572485 2.3143124214326387 7 1.1548688884144331 2.237491001686848 8 0.848197822247239 1.8780956883306652 9 0.7336814114632868 1.8275976921341313 >10 3.5888278566021623 18.218495159713342 >50 0.3416082762368743 6.722680046844949 >100 0.25620620717765574 14.032533602655981 >500 0.019409561149822403 3.6181277062091044 >1k 0.023291473379786883 13.702147776476528 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 3422 1.8383419466440323 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 3335 1.7916044395259636 No Hit ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 3108 1.669657150838589 No Hit ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 2789 1.498286291405671 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2542 1.3655947482083957 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1994 1.0712021746371128 No Hit ATCAACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 1845 0.9911574785383517 No Hit ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1477 0.7934631955561763 No Hit ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 1443 0.775197962889345 No Hit ATCAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1324 0.7112696485554351 No Hit ATCAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1123 0.6032898907309316 No Hit ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 1104 0.5930828489465259 No Hit ATCAACGGGGCCCAACTTCACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGA 964 0.51787306737722 No Hit ATCAACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 868 0.46630064572969604 No Hit ATCAACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 845 0.45394475304331006 No Hit ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 749 0.40237233139578615 No Hit ATCAACGGGATCTCAGCCTACTAGAAACCAGTCTGGGATGGACATGATGGGCTTCT 641 0.3443533570423216 No Hit ATCAACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 551 0.29600421174776786 No Hit ATCAACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 548 0.29439257357128273 No Hit ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 532 0.2857971699633621 No Hit ATCAACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 528 0.28364831906138194 No Hit ATCAACGGGATTGTCCTCTCTGGTGGTTCAACCATGTTCAGGGACTTTGGACGTCG 509 0.27344127727697615 No Hit ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 498 0.2675319372965307 No Hit ATCAACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAG 486 0.2610853845905902 No Hit ATCAACGGGAAGTGGAGTATATTGAATATTGAAGTAGACTTCAGGTTGTTTTTTGG 484 0.2600109591396001 No Hit ATCAACGGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC 465 0.24980391735519433 No Hit ATCAACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 443 0.2379852373943034 No Hit ATCAACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 415 0.22294328108044223 No Hit ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 409 0.219720004727472 No Hit ATCAACGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 401 0.21542230292351164 No Hit ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 399 0.21434787747252157 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC 392 0.2105873883940563 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 373 0.20038034660965048 No Hit ATCAACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 362 0.19447100662920502 No Hit ATCAACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 352 0.18909887937425462 No Hit ATCAACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 346 0.18587560302128436 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 345 0.18533839029578933 No Hit ATCAACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 343 0.18426396484479923 No Hit ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.1772801994133637 No Hit ATCAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC 314 0.16868479580544304 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 301 0.16170103037400752 No Hit ATCAACGGGGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTGC 299 0.16062660492301742 No Hit ATCAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 295 0.15847775402103725 No Hit ATCAACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 291 0.1563289031190571 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTTCC 283 0.15203120131509676 No Hit ATCAACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 283 0.15203120131509676 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC 274 0.14719628678564137 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCG 273 0.14665907406014633 No Hit ATCAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 258 0.13860088317772074 No Hit ATCAACGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC 255 0.13698924500123558 No Hit ATCAACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 253 0.1359148195502455 No Hit ATCAACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA 244 0.13107990502079012 No Hit ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC 243 0.1305426922952951 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 240 0.12893105411880995 No Hit ATCAACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 236 0.12678220321682981 No Hit ATCAACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 234 0.12570777776583972 No Hit ATCAACGGGGGGGCTGGAGAAATAGCTCAGCAGTTAAGAGCTCTGACTGCTCTTCC 232 0.12463335231484964 No Hit ATCAACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC 228 0.12248450141286947 No Hit ATCAACGGGGTGCATCAACGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCC 225 0.12087286323638434 No Hit ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCAACTGCTCTTCCA 223 0.11979843778539426 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTC 218 0.11711237415791904 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTCCTCTTCC 216 0.11603794870692896 No Hit ATCAACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 215 0.11550073598143393 No Hit ATCAACGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA 214 0.11496352325593888 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGCGTGGTTAAGAGCACTGACTGCTCTTC 209 0.1122774596284637 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCC 208 0.11174024690296863 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 208 0.11174024690296863 No Hit ATCAACGGGGGGCTAGAGAGATGGCTCAGCAGTTAAGAGCACTGACTTCTCTTCCA 205 0.1101286087264835 No Hit ATCAACGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT 202 0.1085169705499984 No Hit ATCAACGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCG 202 0.1085169705499984 No Hit ATCAACGGGAGTCTTTTGTATTTTTGATTATTAAGTAAAACTTGCTTTTATTTTAA 200 0.10744254509900832 No Hit ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 198 0.10636811964801823 No Hit ATCAACGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA 198 0.10636811964801823 No Hit ATCAACGGGAACTTTAAACGATAAAAGTACTATCCAATAGGAGATTTATTCTTTCA 195 0.1047564814715331 No Hit ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCC 194 0.10421926874603807 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC 194 0.10421926874603807 No Hit ATCAACGGGATGGAGCTCGCGCTTCCCGACTTCCTCAAGGCCTTCTACGAGTGCCT 194 0.10421926874603807 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCTGACTGCTCTTCC 190 0.10207041784405788 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0010744254509900831 0.0 0.0 0.0 0.0 11 0.0021488509019801662 0.0 0.0 0.0 0.0 12 0.0021488509019801662 0.0 0.0 0.0 0.0 13 0.0026860636274752075 0.0 0.0 0.0 0.0 14 0.003760489078465291 0.0 0.0 0.0 0.0 15 0.003760489078465291 0.0 0.0 0.0 0.0 16 0.0042977018039603325 0.0 0.0 0.0 0.0 17 0.0042977018039603325 0.0 0.0 0.0 0.0 18 0.0042977018039603325 0.0 0.0 0.0 0.0 19 0.0042977018039603325 0.0 0.0 0.0 0.0 20 0.005372127254950415 0.0 0.0 0.0 0.0 21 0.0069837654314355395 0.0 0.0 0.0 0.0 22 0.008058190882425624 0.0 0.0 0.0 0.0 23 0.009132616333415706 0.0 0.0 0.0 0.0 24 0.011281467235395873 0.0 0.0 0.0 0.0 25 0.011281467235395873 0.0 0.0 0.0 0.0 26 0.011281467235395873 0.0 0.0 0.0 0.0 27 0.011818679960890914 0.0 0.0 0.0 0.0 28 0.0284722744512372 0.0 0.0 0.0 0.0 29 0.0284722744512372 0.0 0.0 0.0 0.0 30 0.032769976255197536 0.0 0.0 0.0 0.0 31 0.03384440170618762 0.0 0.0 0.0 0.0 32 0.03545603988267274 0.0 0.0 0.0 0.0 33 0.03599325260816778 0.0 0.0 0.0 0.0 34 0.03760489078465291 0.0 0.0 0.0 0.0 35 0.0478119325690587 0.0 0.0 0.0 0.0 36 0.04942357074554382 0.0 0.0 0.0 0.0 37 0.04996078347103886 0.0 0.0 0.0 0.0 38 0.050497996196533904 0.0 0.0 0.0 0.0 39 0.051035208922028945 0.0 0.0 0.0 0.0 40 0.05425848527499919 0.0 0.0 0.0 0.0 41 0.055332910725989275 0.0 0.0 0.0 0.0 42 0.05748176162796945 0.0 0.0 0.0 0.0 43 0.05748176162796945 0.0 0.0 0.0 0.0 44 0.05801897435346449 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTCG 70 0.0 50.289127 50 GGACGTC 70 0.0 50.289127 49 TTCTAGA 15 0.00823014 50.289124 50 CGAGTTT 25 2.0108366E-5 50.289124 50 CTATCGG 15 0.00823014 50.289124 50 CCGAGTT 25 2.0108366E-5 50.289124 49 ATCCCCT 15 0.00823014 50.289124 49 CTTCTAG 15 0.00823014 50.289124 49 ACGCACA 80 0.0 50.275494 47 CGCACAG 85 0.0 50.27549 48 TGAAGGG 15 0.00823897 50.275482 48 ACTAATT 15 0.00823897 50.275482 48 CATCCCC 15 0.00823897 50.275482 48 ATTTATT 25 2.0173014E-5 50.261864 44 TTTGGAC 70 0.0 50.261864 46 GTTAATG 20 4.068479E-4 50.26186 46 TCGGGAC 15 0.008247807 50.261856 45 TACGAGT 15 0.008247807 50.261856 46 TCATCTC 15 0.008247807 50.261856 45 TTCTACG 15 0.0082566505 50.248238 43 >>END_MODULE