FastQCFastQC Report
Tue 31 May 2016
SRR212602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96599
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT30623.1698050704458636No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC18981.964823652418762No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC16101.6666839201233967No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA14871.5393534094555845No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11721.2132630772575286No Hit
CACAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10281.0641932111098458No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8260.8550813155415689No Hit
CACAAGGGGGAGTAAGATACTTTCAGTTTTGTTCATTTTTCAGAAGCTACTGTAAAGA7920.8198842638122548No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6040.6252652718972246No Hit
CACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5380.5569415832462034No Hit
CACAAGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5140.5320966055549229No Hit
CACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5130.5310613981511195No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4660.482406650172362No Hit
CACAAGGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4190.4337519021936045No Hit
CACAAGGGGGGGTATATTAGCCTGGGCTGGCATTTGTGTTCTCTTAGTGTCTGTATAA3830.3964844356566838No Hit
CACAAGGGGAGTCAAAACGCCTTGTTAAAAAGAAACGAGCTTTAAAATCATGTTTATA3790.39234360604147045No Hit
CACAAGGGGACCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3770.39027319123386367No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3070.3178086729676291No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2940.30435097671818545No Hit
CACAAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2940.30435097671818545No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGTTCTTCAGA2920.3022805619105788No Hit
CACAAGGGGACAGGGCAGGGTGTTGGGTAGGGTTTCTAAGCTATAGGTGTGTTACCCT2550.2639778879698548No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC2510.2598370583546413No Hit
CACAAGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA2490.25776664354703466No Hit
CACAAGGGGAAGATAAAAGAAAAACAGAAGAGGAAGATAAATCACAATCATTTATGCA2430.25155539912421454No Hit
CACAAGGGGGGGGCTGGTGAGATGACTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG2420.2505201917204112No Hit
CACAAGGGGATGCTAACAAGATACGCTCGGACCCAGTTTGTCAGTCCTTGGAGAGAGG2410.24948498431660782No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2360.2443089472975911No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA2350.2432737398937877No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2280.23602728806716425No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2230.23085125104814747No Hit
CACAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2170.22464000662532738No Hit
CACAAGGGGAGCAGTTTCCTCAACCAGCGCTGTCCCACCGGTGGCTCCGTGGTTTCTC2130.22049917701011398No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2120.21946396960631065No Hit
CACAAGGGGACGTCTGGCGGGGAAACTCACCGCCTCAGCAGCTCAGCTAAAACCCTGA2050.21221751777968714No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1960.202900651145457No Hit
CACAAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1890.19565419931883354No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.19565419931883354No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA1870.19358378451122682No Hit
CACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1850.19151336970362012No Hit
CACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1840.19047816229981676No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1810.18737254008840673No Hit
CACAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG1730.1790908808579799No Hit
CACAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1680.17391484383896313No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1620.16770359941614302No Hit
CACAAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1590.16459797720473296No Hit
CACAAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1590.16459797720473296No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCCAA1470.15217548835909273No Hit
CACAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1460.1511402809552894No Hit
CACAAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGAA1420.146999451340076No Hit
CACAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1410.14596424393627264No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1400.14492903653246927No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA1390.14389382912866594No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1340.13871779210964916No Hit
CACAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1310.1356121698982391No Hit
CACAAGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCTAAG1280.13250654768682907No Hit
CACAAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1270.1314713402830257No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA1270.1314713402830257No Hit
CACAAGGGGTGCCCTCCGTCCGATATGCCCACACTCCAGCTGCTACTGGTTCCCTCCT1240.12836571807161565No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAA1220.12629530326400895No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCCCTTCCGA1200.12422488845640223No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1170.12111926624499218No Hit
CACAAGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1160.12008405884118883No Hit
CACAAGGGGGTAGCCATGACAACTTGCATTTTCTTAGTTGTGCATCCCGTGCTTGCAC1160.12008405884118883No Hit
CACAAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1160.12008405884118883No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG1160.12008405884118883No Hit
CACAAGGGGGCAGAGTGCAGTGCTCACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGT1150.11904885143738549No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1150.11904885143738549No Hit
CACAAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1150.11904885143738549No Hit
CACAAGGGGGGTCTTGAGTTCAAATCCCAACAACCACATGGTGGCTTAGAACCTTCTA1140.11801364403358212No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA1130.11697843662977878No Hit
CACAAGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1120.11594322922597543No Hit
CACAAGGGGATTGTAAATGTATACGTTGGAACTAGACTCCACAACTATACATTTTGGT1100.11387281441836872No Hit
CACAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1090.11283760701456538No Hit
CACAAGGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTCCA1090.11283760701456538No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1070.11076719220695866No Hit
CACAAGGGGGGGCTGGAGAGATGGCTTGGCGGTTAAGAGCACTGGCTGCTCTTCCGAA1030.10662636259174527No Hit
CACAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1020.1055911551879419No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTCTGA1020.1055911551879419No Hit
CACAAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA1000.10352074038033521No Hit
CACAAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC990.10248553297653185No Hit
CACAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG990.10248553297653185No Hit
CACAAGGGGGGGCTGGGGAGTTAGCCCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA980.1014503255727285No Hit
CACAAGGGGAGTGGAGTTTACCCAGGAGAATCCCTTTTGCCGGTTTAAGGCTGTAGGC970.10041511816892515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC251.6327851E-552.0591649
TGCGCTA150.007167884652.0591652
TATATGG251.6327851E-552.0591651
GGCCGCT150.007167884652.0591649
CTCTTAC150.007167884652.0591650
GGTTCTT150.007167884652.0591650
CTTAGTC150.007167884652.0591650
AAGGGAG353.799505E-852.0591652
GTTTATA401.8462742E-952.0591652
GAGTTAC203.4126113E-452.0591652
TACAAGC150.007167884652.0591649
GCTGTAG150.007167884652.0591650
CTTACTG150.007167884652.0591652
CTAACGC251.6327851E-552.0591652
GACGAAG150.007167884652.0591650
GACGAAC203.4126113E-452.0591652
GTGGTTT203.4126113E-452.0591649
ATCGCCA150.007167884652.0591652
AAAGTCT251.6327851E-552.0591651
CTCATCA251.6327851E-552.0591651
Produced by FastQC (version 0.11.5)