FastQCFastQC Report
Tue 31 May 2016
SRR212601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96948
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT30383.1336386516483064No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17831.8391302553946447No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC16241.6751248091760533No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13941.4378842265957008No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC10851.1191566613029664No Hit
CACAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10171.0490159673226884No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8880.9159549449189256No Hit
CACAAGGGGGAGTAAGATACTTTCAGTTTTGTTCATTTTTCAGAAGCTACTGTAAAGA7980.8231216734744399No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6370.6570532656681932No Hit
CACAAGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5490.5662829558113628No Hit
CACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5060.5219292816767752No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4980.5136774353261543No Hit
CACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4790.4940793002434295No Hit
CACAAGGGGGGGTATATTAGCCTGGGCTGGCATTTGTGTTCTCTTAGTGTCTGTATAA4110.4239386062631514No Hit
CACAAGGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4000.4125923175310475No Hit
CACAAGGGGAGTCAAAACGCCTTGTTAAAAAGAAACGAGCTTTAAAATCATGTTTATA3820.39402566324215044No Hit
CACAAGGGGACCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3510.3620497586334942No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3230.33316829640632095No Hit
CACAAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2990.30841275735445806No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGTTCTTCAGA2990.30841275735445806No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2960.30531831497297524No Hit
CACAAGGGGACAGGGCAGGGTGTTGGGTAGGGTTTCTAAGCTATAGGTGTGTTACCCT2380.2454924289309733No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.2444609481371457No Hit
CACAAGGGGATGCTAACAAGATACGCTCGGACCCAGTTTGTCAGTCCTTGGAGAGAGG2340.24136650575566285No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2290.2362091017865247No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2280.2351776209926971No Hit
CACAAGGGGAAGATAAAAGAAAAACAGAAGAGGAAGATAAATCACAATCATTTATGCA2270.23414614019886948No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2190.22589429384824855No Hit
CACAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2170.2238313322605933No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2160.22279985146676567No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2090.21557948590997236No Hit
CACAAGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA2070.21351652432231713No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC2070.21351652432231713No Hit
CACAAGGGGGGGGCTGGTGAGATGACTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG2040.21042208194083428No Hit
CACAAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2000.20629615876552376No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA1990.20526467797169615No Hit
CACAAGGGGAGCAGTTTCCTCAACCAGCGCTGTCCCACCGGTGGCTCCGTGGTTTCTC1950.20113875479638568No Hit
CACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1760.18154061971366092No Hit
CACAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG1750.1805091389198333No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1750.1805091389198333No Hit
CACAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1680.17328877336303997No Hit
CACAAGGGGACGTCTGGCGGGGAAACTCACCGCCTCAGCAGCTCAGCTAAAACCCTGA1650.17019433098155712No Hit
CACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1650.17019433098155712No Hit
CACAAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1640.1691628501877295No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1600.165036927012419No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA1540.15884804224945331No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1540.15884804224945331No Hit
CACAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1480.15265915748648762No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA1390.14337583034203905No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1350.13924990716672855No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCCAA1320.1361554647852457No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCCCTTCCGA1310.13512398399141806No Hit
CACAAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGAA1310.13512398399141806No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA1310.13512398399141806No Hit
CACAAGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1290.13306102240376283No Hit
CACAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1280.1320295416099352No Hit
CACAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1270.1309980608161076No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1260.12996658002227998No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1260.12996658002227998No Hit
CACAAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1230.12687213764079713No Hit
CACAAGGGGGCAGAGTGCAGTGCTCACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGT1220.1258406568469695No Hit
CACAAGGGGTGCCCTCCGTCCGATATGCCCACACTCCAGCTGCTACTGGTTCCCTCCT1210.12480917605314189No Hit
CACAAGGGGGGTCTTGAGTTCAAATCCCAACAACCACATGGTGGCTTAGAACCTTCTA1190.12274621446548666No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAA1150.11862029129017616No Hit
CACAAGGGGGTAGCCATGACAACTTGCATTTTCTTAGTTGTGCATCCCGTGCTTGCAC1130.11655732970252093No Hit
CACAAGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCTAAG1130.11655732970252093No Hit
CACAAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1130.11655732970252093No Hit
CACAAGGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTCCA1100.11346288732103808No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA1090.11243140652721047No Hit
CACAAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA1070.11036844493955522No Hit
CACAAGGGGATTGTAAATGTATACGTTGGAACTAGACTCCACAACTATACATTTTGGT1070.11036844493955522No Hit
CACAAGGGGGGGCTGGGGAGTTAGCCCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA1060.1093369641457276No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1050.10830548335189999No Hit
CACAAGGGGAGTGGAGTTTACCCAGGAGAATCCCTTTTGCCGGTTTAAGGCTGTAGGC1050.10830548335189999No Hit
CACAAGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1020.10521104097041714No Hit
CACAAGGGGAGTCCTGTGCTGCCGCTGTCGCCGCTGCGCTTTGGTCTCGGAGCTGGCA1020.10521104097041714No Hit
CACAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAG1010.1041795601765895No Hit
CACAAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1010.1041795601765895No Hit
CACAAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1000.10314807938276188No Hit
CACAAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1000.10314807938276188No Hit
CACAAGGGGAGGCAGCTGACACTCGCAGTTATTGCAGGAATGAACGGCAGAGTATTTA980.10108511779510665No Hit
CACAAGGGGGAAAAAAATGAGAACAAGCTGTTAGTGAAAAAAATGAAACACTGTCTCT970.10005363700127903No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC970.10005363700127903No Hit
CACAAGGGGGTAAAGGGTGTCTTTAGATCACTGTTTCGTCGGTATAGGTAAATGACAT970.10005363700127903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATC150.00714271752.1055927
GCCCACA150.00714271752.1055927
ACCATGT150.00714271752.1055925
GCTGGGT401.8317223E-952.1055926
GTGAGTG150.00714271752.1055927
AGAATCC203.39767E-452.1055927
AACTTGG150.00714271752.1055926
GCTGTTA150.00714271752.1055927
TATCTTC150.00714271752.1055927
CCATATT150.00714271752.1055925
GCTTGAT150.00714271752.1055926
ATCACTG150.00714271752.1055927
GATCACT150.00714271752.1055926
TACCACT150.00714271752.1055925
GTTATCT150.00714271752.1055925
AAGCCCG150.00714271752.1055925
TAGATCA150.00714271752.1055924
ACAGCTC150.00714271752.1055927
TCGGACC353.7731297E-852.10558727
CTTCATT150.007186706652.02480730
Produced by FastQC (version 0.11.5)