##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212601_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96948 Sequences flagged as poor quality 0 Sequence length 58 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.042981804678796 39.0 38.0 40.0 35.0 40.0 2 37.3647728679292 39.0 37.0 40.0 33.0 40.0 3 37.48718900854066 39.0 38.0 40.0 33.0 40.0 4 37.296117506292035 39.0 37.0 40.0 33.0 40.0 5 37.82882576226431 39.0 38.0 40.0 35.0 40.0 6 37.08227090811569 39.0 36.0 39.0 33.0 40.0 7 38.05321409415357 39.0 38.0 40.0 35.0 40.0 8 38.062187977059864 39.0 38.0 40.0 35.0 40.0 9 38.01868011717622 39.0 38.0 40.0 35.0 40.0 10 37.01705037752197 39.0 36.0 39.0 33.0 40.0 11 36.84205966084912 39.0 35.0 39.0 32.0 40.0 12 36.68837933737674 39.0 35.0 39.0 31.0 40.0 13 36.66717209225564 39.0 35.0 39.0 31.0 40.0 14 36.82248215538227 39.0 36.0 39.0 32.0 40.0 15 36.90527911870281 39.0 36.0 39.0 33.0 40.0 16 36.428456492140114 38.0 35.0 39.0 31.0 40.0 17 35.98220695630647 38.0 35.0 39.0 30.0 40.0 18 35.91852333209555 38.0 35.0 39.0 30.0 40.0 19 35.66833766555267 38.0 35.0 39.0 29.0 40.0 20 35.667646573420804 38.0 35.0 39.0 29.0 40.0 21 35.31053760778974 38.0 34.0 39.0 28.0 40.0 22 35.28767999339852 38.0 34.0 39.0 28.0 40.0 23 35.174557494739446 38.0 34.0 39.0 28.0 40.0 24 34.94106118744069 38.0 33.0 39.0 27.0 40.0 25 34.946847794694065 38.0 33.0 39.0 28.0 40.0 26 34.53420390312333 38.0 33.0 39.0 26.0 40.0 27 34.03528695795684 37.0 33.0 39.0 25.0 40.0 28 33.88395841069439 37.0 33.0 39.0 25.0 40.0 29 34.061765069934395 37.0 33.0 39.0 26.0 40.0 30 33.82342080290465 37.0 33.0 39.0 25.0 40.0 31 33.00306349795767 36.0 32.0 38.0 22.0 40.0 32 32.964125097990674 36.0 32.0 38.0 22.0 40.0 33 32.853901060362254 36.0 31.0 38.0 23.0 40.0 34 31.711123488880638 35.0 29.0 38.0 17.0 39.0 35 31.265699137682056 35.0 29.0 38.0 16.0 39.0 36 32.52297107727854 36.0 32.0 39.0 19.0 40.0 37 32.230752568387175 36.0 31.0 38.0 18.0 40.0 38 32.00030944423815 36.0 31.0 38.0 18.0 40.0 39 32.104086726905145 36.0 31.0 38.0 18.0 40.0 40 31.885031150719975 36.0 31.0 38.0 17.0 40.0 41 32.144479514791435 36.0 32.0 39.0 14.0 40.0 42 31.873457936213228 36.0 31.0 39.0 13.0 40.0 43 31.69259809382349 36.0 31.0 39.0 10.0 40.0 44 31.622591492346412 36.0 31.0 39.0 10.0 40.0 45 31.37518050913892 36.0 30.0 38.0 2.0 39.0 46 30.398956141436646 35.0 29.0 38.0 2.0 39.0 47 30.34077030985683 35.0 29.0 38.0 2.0 39.0 48 30.35951231588068 35.0 29.0 38.0 2.0 39.0 49 30.382452448735403 35.0 29.0 38.0 2.0 39.0 50 30.104746874613195 35.0 29.0 38.0 2.0 39.0 51 29.826989726451295 35.0 29.0 38.0 2.0 39.0 52 29.571636341131327 35.0 29.0 38.0 2.0 39.0 53 29.317582621611585 35.0 28.0 38.0 2.0 39.0 54 29.104014523249578 35.0 28.0 38.0 2.0 39.0 55 29.067830177002104 35.0 28.0 38.0 2.0 39.0 56 28.974635887279778 35.0 28.0 38.0 2.0 39.0 57 28.407878450303254 35.0 27.0 38.0 2.0 39.0 58 27.867506292032843 34.0 25.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.3415704493091596 1 2 -1.658852867639105 1 3 -1.8262505305342458 1 4 -2.159988815068999 1 5 -1.2933387392367095 1 6 -2.1342318180427426 1 7 -1.6269840568605645 1 8 -1.5395912887983982 1 9 -1.6437163959649226 1 10 -2.526567992967699 1 11 -1.22970658317859 1 12 -1.6631199220588115 1 13 -1.8383606945730548 1 14 -1.412968662831922 1 15 -2.5605281674407863 1 16 -2.5159378626098388 1 17 -2.115157028262807 1 18 -1.4366433594410353 1 19 -1.1892988363877706 1 20 -2.8009037868884334 1 21 -1.9684118829665493 1 22 -1.5040797402120774 1 23 -2.374363656414367 1 24 -1.5487664541112949 1 25 -1.0646612692332056 1 26 -1.7420791242870024 1 27 -1.321083970904887 1 28 -2.1296877179967133 1 29 -1.4168376014445627 1 30 -1.758021709229169 1 31 -1.0616524592097 1 32 -0.3801023258000953 1 33 -1.691239099416947 1 34 -1.3320481597177043 1 35 -0.7975313169226794 1 36 -2.077551266269463 1 37 -1.6691116447370682 1 38 -1.5106719340919632 1 39 -2.0547013931205385 1 40 -1.429661863796838 1 41 -0.4774042507857388 1 42 -1.7371361900285756 1 43 -0.9795266200008257 1 44 -0.1033085255651649 1 45 -0.3939594229725394 1 46 -1.2097690020966105 1 47 -0.8189745990021251 1 48 -0.29080518803069566 1 49 -0.30900939264241956 1 50 -0.3306245624327495 1 51 0.840552490832625 1 52 0.1599768952098799 1 53 0.8409697372364029 1 54 0.8066644720692189 1 55 1.4794990375456614 1 56 0.2182011817530558 1 57 -1.197635591996601 1 58 0.4854302039440306 2 1 -0.33176652774052684 2 2 -0.33905828313864106 2 3 -0.6941310160617888 2 4 -0.2981774238458499 2 5 -0.416588038956597 2 6 -1.1419348992752347 2 7 -0.3020540848717701 2 8 -0.6934194867442471 2 9 -0.3594026704747293 2 10 0.3525169743525609 2 11 -0.671116480470836 2 12 -0.32488462794116657 2 13 -1.0913952417252517 2 14 -1.4433141343538622 2 15 -0.46622377901874756 2 16 -0.9771890297433572 2 17 -2.0988171589817597 2 18 -1.2022829486100335 2 19 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118 15 -0.9046551515677663 118 16 -0.9089537356257154 118 17 -1.039601472707254 118 18 -0.4093417721394488 118 19 -1.0153305824195158 118 20 -0.09042759641224052 118 21 -0.6407928353474972 118 22 -0.8259845021168388 118 23 -0.8823001643508732 118 24 -0.27384581919066164 118 25 -0.09259777716971485 118 26 -0.3681108703187519 118 27 -0.06775063757154953 118 28 -0.5696877179967146 118 29 -0.7641391887461495 118 30 -1.3097677409752038 118 31 0.46088722332998344 118 32 -0.12740391310168064 118 33 -0.30330259148043837 118 34 -1.654587842257385 118 35 0.5345321751408108 118 36 -0.582313171031366 118 37 -0.7541910098164344 118 38 -1.0389259023459338 118 39 -0.26359028200942447 118 40 -0.750296784431761 118 41 -0.5288328222143122 118 42 -1.505390158282541 118 43 -0.32682820730241247 118 44 -0.5464831287397693 118 45 -0.3076102166233312 118 46 -0.4345309068585159 118 47 0.6076920676645408 118 48 0.2653852881597807 118 49 -0.05186653549956333 118 50 -0.2887198005279892 118 51 1.0272191574992888 118 52 1.358072133305118 118 53 0.6320808483475133 118 54 0.9469819323866773 118 55 1.5639434819901048 118 56 1.3908995944514686 118 57 0.6290310746700669 118 58 1.5241603626741878 119 1 -0.17039927813798528 119 2 -0.49883570762194296 119 3 -0.37537107965479066 119 4 -0.9420565971367836 119 5 -0.3506102965082647 119 6 -0.7552099390208653 119 7 -0.69712562700213 119 8 -0.6127358619429728 119 9 -0.3959686482171776 119 10 -1.0520935184932227 119 11 -0.8734217268937314 119 12 -1.636092895031787 119 13 -1.1255764817888405 119 14 -1.8526941025573578 119 15 -1.9320425389551588 119 16 -1.159224005895986 119 17 -0.5821239952297717 119 18 -0.6196120424097202 119 19 -0.7236188707078028 119 20 -1.4212384072230577 119 21 -1.9838558984105603 119 22 -1.1849034210357559 119 23 -3.0399578220085317 119 24 -0.616548521893364 119 25 -0.7843094888814264 119 26 -0.8664892486971283 119 27 -0.3931560429769547 119 28 -1.688246276555276 119 29 -0.6392743238812884 119 30 -1.7762542274616848 119 31 -2.180914578471821 119 32 -1.6565931022908735 119 33 -1.701320609498456 119 34 -0.9515247791943224 119 35 -1.683846203237568 119 36 -0.7976284863466852 119 37 -1.164821640447066 119 38 -0.1726195960396275 119 39 -0.30395064236978797 119 40 -0.6385850727200477 119 41 -1.2335175068989948 119 42 -0.1062910591834445 119 43 -0.7515128919870975 119 44 -0.3821588044154467 119 45 0.35743482842171304 119 46 1.4575411652135593 119 47 0.3116560316285053 119 48 0.4511510539255461 119 49 0.10218751855449071 119 50 -0.07322430503249322 119 51 -0.4342222839421517 119 52 -0.0613873261543425 119 53 -0.665036268769601 119 54 -0.6744595090547634 119 55 -1.251732193685573 119 56 -0.2654968019449271 119 57 -0.7201581145191227 119 58 -0.23872252020869666 120 1 -1.5167116922470072 120 2 -2.8859177048734352 120 3 -2.310781065912238 120 4 -2.713930165620525 120 5 -2.2271563340712532 120 6 -3.072892027889395 120 7 -2.719800844592605 120 8 -2.7774174927263005 120 9 -2.5533209157389365 120 10 -2.4794870136833254 120 11 -2.2735591524548866 120 12 -2.341086023753725 120 13 -3.0016644341479832 120 14 -2.2832941941744025 120 15 -2.3017172984604244 120 16 -2.59234356613419 120 17 -2.757341585701603 120 18 -3.052731602647924 120 19 -2.6697938592556767 120 20 -3.407037765903766 120 21 -2.2880244737655815 120 22 -2.5710692478795494 120 23 -2.6393623112435307 120 24 -2.1850322598686276 120 25 -2.753388737621691 120 26 -2.5728566330306144 120 27 -1.2080896206223954 120 28 -2.2878798083921943 120 29 -1.91939342603429 120 30 -1.298242317246384 120 31 -0.5542540196078676 120 32 -1.967403913101684 120 33 -1.5957319700115136 120 34 -1.2744748479071042 120 35 -1.8647898587574936 120 36 -0.3608442444776969 120 37 -1.5709141736582417 120 38 -0.5184739249448036 120 39 -1.2769236153427599 120 40 -0.881370230759444 120 41 -1.1787763250391698 120 42 -1.953977728904011 120 43 -2.3069412016526947 120 44 -2.181059399926209 120 45 -2.72433338046514 120 46 -3.3631184774799827 120 47 -4.502590418211167 120 48 -3.837213581896716 120 49 -3.85864619651651 120 50 -5.709962738381094 120 51 -4.317639599562856 120 52 -4.890063459915222 120 53 -3.940574518884123 120 54 -3.964656485692416 120 55 -3.550632789196335 120 56 -3.2052585976389274 120 57 -3.4801214677028156 120 58 -3.5140317277212922 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114.0 3 9.0 4 34.0 5 35.0 6 68.0 7 105.0 8 97.0 9 176.0 10 243.0 11 253.0 12 350.0 13 375.0 14 404.0 15 510.0 16 528.0 17 572.0 18 634.0 19 658.0 20 754.0 21 760.0 22 987.0 23 1172.0 24 1250.0 25 1183.0 26 1341.0 27 1621.0 28 1943.0 29 2265.0 30 2792.0 31 3356.0 32 4296.0 33 5366.0 34 6837.0 35 8664.0 36 11404.0 37 14668.0 38 13822.0 39 7296.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 61.948673515699134 22.650286751660683 3.4802161983743862 11.920823534265793 11 36.67636258612865 17.9003176960845 13.873416676981476 31.549903040805383 12 38.51446136072946 11.138961092544458 28.41420142756942 21.93237611915666 13 20.16544951932995 15.355654577711764 35.864587201386314 28.61430870157198 14 15.979700457977472 20.0963403061435 45.0478607088336 18.876098527045425 15 25.504394108181707 14.45104592152494 42.14939967817799 17.89516029211536 16 28.507034699013907 15.093658456079547 32.4245987539712 23.974708090935344 17 25.80558649997937 16.55629822172711 22.99480133679911 34.643313941494405 18 30.382162976414023 15.601205092754999 25.28734446255752 28.729287468273455 19 28.046994264966784 24.131493171597146 26.799933985229195 21.021578578206874 20 33.39728514255065 19.68065354623097 26.561661921855016 20.36039938936337 21 30.851590543384084 20.684284358625245 25.932458637620165 22.53166646037051 22 34.34901904152827 18.194665071276795 27.8227054236379 19.633610463557034 23 40.07199735940917 19.61154433304452 23.053595742047285 17.26286256549903 24 37.85911018041902 21.862782517201186 19.653191116245964 20.624916186133834 25 31.405495729669514 23.964393282997072 17.49185130172876 27.138259685604655 26 28.61224868734591 19.116782372783444 23.736086898216442 28.534882041654203 27 22.427896298900734 18.798151001540834 22.97390926670872 35.80004343284971 28 25.248096721615877 28.43673275701995 22.496853659039797 23.818316862324373 29 33.198180073663686 19.412547587359562 22.696462286050327 24.69281005292643 30 25.294688383807106 16.89890794987717 28.443880184141534 29.36252348217419 31 33.61219710958211 15.94887612052692 25.69501036713052 24.74391640276044 32 41.208638141128155 12.464016343544609 27.474489006283598 18.852856509043633 33 41.599083761530366 12.565261354959864 27.699704905177576 18.135949978332196 34 28.58675801442442 16.116550934285332 36.93806168037227 18.358629370917985 35 24.254713184533944 28.634079394070728 35.35172171831306 11.759485703082273 36 26.79966561051882 33.078756979348356 24.93678593912873 15.184791471004097 37 30.963440061105263 26.277327057657768 27.52626906958981 15.232963811647156 38 27.00875345802882 28.77596102233783 27.198686981295676 17.016598538337668 39 31.716256116949886 23.90101381346658 25.206995519398735 19.1757345501848 40 28.82431874483898 23.112097440132125 25.847440132122212 22.21614368290669 41 28.621718438683967 22.7569760599585 27.930048416901528 20.69125708445601 42 26.229508196721312 21.812156749390923 28.22191022835198 23.73642482553578 43 26.252877359951277 19.135604942349577 26.62757930158861 27.98393839611053 44 24.38656798075831 16.473114283649725 28.906919368657935 30.23339836693403 45 29.170236264360106 17.84317166066286 27.574497073790035 25.412095001186998 46 26.73658783330409 21.648377739467954 31.242580338801083 20.372454088426878 47 24.94475309279415 18.168487577190774 32.37778558004089 24.50897374997418 48 28.313576473503783 16.772334889099998 34.44095658997976 20.473132047416463 49 29.206080383328516 16.657028377875996 29.41054979553059 24.7263414432649 50 18.116750325339282 17.057073805540064 32.779740141703336 32.04643572741732 51 17.803961254879283 15.992688820504346 37.464631652863545 28.738718271752823 52 17.7675667303423 17.61474521142031 40.892147245598636 23.725540812638755 53 15.956589979761265 16.833257610177192 44.08946346701912 23.12068894304242 54 14.46116660296867 19.506047866461458 39.922116288438296 26.110669242131575 55 18.225308163623776 17.572300920616225 34.33764194125001 29.864748974509986 56 18.580330477735636 20.90855542580785 26.313178807262656 34.19793528919385 57 23.234786562367603 20.763022743947175 29.266427619273973 26.73576307441125 58 21.09711616949608 28.006530207375413 28.118122358727437 22.778231264401068 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 19.5 10 39.0 11 67.5 12 96.0 13 100.5 14 105.0 15 112.5 16 245.5 17 371.0 18 548.0 19 725.0 20 623.0 21 521.0 22 590.5 23 808.0 24 956.0 25 1204.5 26 1453.0 27 1638.5 28 1898.0 29 1972.0 30 1873.0 31 1774.0 32 1592.5 33 1411.0 34 1884.5 35 2258.0 36 2158.0 37 1521.0 38 884.0 39 903.5 40 923.0 41 880.5 42 1016.5 43 1195.0 44 1322.5 45 1450.0 46 2230.0 47 2366.5 48 1723.0 49 1832.5 50 1942.0 51 2212.5 52 2483.0 53 3561.0 54 4278.0 55 3917.0 56 3975.5 57 4034.0 58 5641.5 59 7966.0 60 8683.0 61 9178.5 62 9674.0 63 11088.5 64 12503.0 65 9144.0 66 4594.0 67 3403.0 68 2846.5 69 2290.0 70 1817.0 71 1344.0 72 1152.5 73 750.5 74 540.0 75 451.5 76 363.0 77 298.5 78 192.5 79 151.0 80 113.5 81 76.0 82 67.5 83 59.0 84 44.5 85 21.5 86 13.0 87 10.0 88 7.0 89 4.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.026818500639518093 19 0.0 20 0.0 21 0.0 22 0.0020629615876552377 23 0.0 24 0.007220365556793332 25 0.0 26 0.007220365556793332 27 0.25477575607542186 28 0.010314807938276188 29 0.021661096670379996 30 0.06807773239262285 31 0.007220365556793332 32 0.029912943021000945 33 0.030944423814828568 34 0.029912943021000945 35 0.040227750959277137 36 0.05673144366051904 37 0.06807773239262285 38 0.07426661715558856 39 0.08870734826917523 40 0.07014069398027808 41 0.08354994430003714 42 0.0825184635062095 43 0.07323513636176095 44 0.07736105953707142 45 0.06704625159879522 46 0.07942402112472666 47 0.11346288732103808 48 0.10727400255807237 49 0.11552584890869332 50 0.12996658002227998 51 0.11346288732103808 52 0.10624252176424476 53 0.10727400255807237 54 0.14131286875438379 55 0.17019433098155712 56 0.1846350620951438 57 0.1805091389198333 58 0.17225729256921235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 96948.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.643685274580186 #Duplication Level Percentage of deduplicated Percentage of total 1 69.38805970149254 19.1814168420184 2 10.921641791044776 6.038288567066881 3 5.130597014925374 4.254858274538928 4 3.074626865671642 3.3997606964558322 5 2.246268656716418 3.1047571894211328 6 1.4888059701492538 2.4693650204233197 7 1.2313432835820897 2.3827206337417994 8 0.8768656716417911 1.9391838923959237 9 0.7574626865671642 1.8845154103230597 >10 4.1940298507462686 20.959689730577217 >50 0.3992537313432836 7.71547633783059 >100 0.25 12.931674712216859 >500 0.01865671641791045 3.484342121549697 >1k 0.022388059701492536 10.25395057144036 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 3038 3.1336386516483064 No Hit CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1783 1.8391302553946447 No Hit CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1624 1.6751248091760533 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1394 1.4378842265957008 No Hit CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1085 1.1191566613029664 No Hit CACAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1017 1.0490159673226884 No Hit CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 888 0.9159549449189256 No Hit CACAAGGGGGAGTAAGATACTTTCAGTTTTGTTCATTTTTCAGAAGCTACTGTAAAGA 798 0.8231216734744399 No Hit CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 637 0.6570532656681932 No Hit CACAAGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 549 0.5662829558113628 No Hit CACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 506 0.5219292816767752 No Hit CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 498 0.5136774353261543 No Hit CACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 479 0.4940793002434295 No Hit CACAAGGGGGGGTATATTAGCCTGGGCTGGCATTTGTGTTCTCTTAGTGTCTGTATAA 411 0.4239386062631514 No Hit CACAAGGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 400 0.4125923175310475 No Hit CACAAGGGGAGTCAAAACGCCTTGTTAAAAAGAAACGAGCTTTAAAATCATGTTTATA 382 0.39402566324215044 No Hit CACAAGGGGACCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 351 0.3620497586334942 No Hit CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 323 0.33316829640632095 No Hit CACAAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 299 0.30841275735445806 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGTTCTTCAGA 299 0.30841275735445806 No Hit CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 296 0.30531831497297524 No Hit CACAAGGGGACAGGGCAGGGTGTTGGGTAGGGTTTCTAAGCTATAGGTGTGTTACCCT 238 0.2454924289309733 No Hit CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 237 0.2444609481371457 No Hit CACAAGGGGATGCTAACAAGATACGCTCGGACCCAGTTTGTCAGTCCTTGGAGAGAGG 234 0.24136650575566285 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 229 0.2362091017865247 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 228 0.2351776209926971 No Hit CACAAGGGGAAGATAAAAGAAAAACAGAAGAGGAAGATAAATCACAATCATTTATGCA 227 0.23414614019886948 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 219 0.22589429384824855 No Hit CACAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 217 0.2238313322605933 No Hit CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 216 0.22279985146676567 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA 209 0.21557948590997236 No Hit CACAAGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA 207 0.21351652432231713 No Hit CACAAGGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC 207 0.21351652432231713 No Hit CACAAGGGGGGGGCTGGTGAGATGACTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG 204 0.21042208194083428 No Hit CACAAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 200 0.20629615876552376 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA 199 0.20526467797169615 No Hit CACAAGGGGAGCAGTTTCCTCAACCAGCGCTGTCCCACCGGTGGCTCCGTGGTTTCTC 195 0.20113875479638568 No Hit CACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 176 0.18154061971366092 No Hit CACAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG 175 0.1805091389198333 No Hit CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 175 0.1805091389198333 No Hit CACAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 168 0.17328877336303997 No Hit CACAAGGGGACGTCTGGCGGGGAAACTCACCGCCTCAGCAGCTCAGCTAAAACCCTGA 165 0.17019433098155712 No Hit CACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 165 0.17019433098155712 No Hit CACAAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA 164 0.1691628501877295 No Hit CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA 160 0.165036927012419 No Hit CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA 154 0.15884804224945331 No Hit CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 154 0.15884804224945331 No Hit CACAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 148 0.15265915748648762 No Hit CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA 139 0.14337583034203905 No Hit CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC 135 0.13924990716672855 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCCAA 132 0.1361554647852457 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCCCTTCCGA 131 0.13512398399141806 No Hit CACAAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGAA 131 0.13512398399141806 No Hit CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA 131 0.13512398399141806 No Hit CACAAGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA 129 0.13306102240376283 No Hit CACAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 128 0.1320295416099352 No Hit CACAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 127 0.1309980608161076 No Hit CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC 126 0.12996658002227998 No Hit CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 126 0.12996658002227998 No Hit CACAAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 123 0.12687213764079713 No Hit CACAAGGGGGCAGAGTGCAGTGCTCACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGT 122 0.1258406568469695 No Hit CACAAGGGGTGCCCTCCGTCCGATATGCCCACACTCCAGCTGCTACTGGTTCCCTCCT 121 0.12480917605314189 No Hit CACAAGGGGGGTCTTGAGTTCAAATCCCAACAACCACATGGTGGCTTAGAACCTTCTA 119 0.12274621446548666 No Hit CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAA 115 0.11862029129017616 No Hit CACAAGGGGGTAGCCATGACAACTTGCATTTTCTTAGTTGTGCATCCCGTGCTTGCAC 113 0.11655732970252093 No Hit CACAAGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCTAAG 113 0.11655732970252093 No Hit CACAAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG 113 0.11655732970252093 No Hit CACAAGGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTCCA 110 0.11346288732103808 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA 109 0.11243140652721047 No Hit CACAAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA 107 0.11036844493955522 No Hit CACAAGGGGATTGTAAATGTATACGTTGGAACTAGACTCCACAACTATACATTTTGGT 107 0.11036844493955522 No Hit CACAAGGGGGGGCTGGGGAGTTAGCCCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA 106 0.1093369641457276 No Hit CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC 105 0.10830548335189999 No Hit CACAAGGGGAGTGGAGTTTACCCAGGAGAATCCCTTTTGCCGGTTTAAGGCTGTAGGC 105 0.10830548335189999 No Hit CACAAGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 102 0.10521104097041714 No Hit CACAAGGGGAGTCCTGTGCTGCCGCTGTCGCCGCTGCGCTTTGGTCTCGGAGCTGGCA 102 0.10521104097041714 No Hit CACAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAG 101 0.1041795601765895 No Hit CACAAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG 101 0.1041795601765895 No Hit CACAAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA 100 0.10314807938276188 No Hit CACAAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT 100 0.10314807938276188 No Hit CACAAGGGGAGGCAGCTGACACTCGCAGTTATTGCAGGAATGAACGGCAGAGTATTTA 98 0.10108511779510665 No Hit CACAAGGGGGAAAAAAATGAGAACAAGCTGTTAGTGAAAAAAATGAAACACTGTCTCT 97 0.10005363700127903 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 97 0.10005363700127903 No Hit CACAAGGGGGTAAAGGGTGTCTTTAGATCACTGTTTCGTCGGTATAGGTAAATGACAT 97 0.10005363700127903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006188884762965714 0.0 0.0 0.0 0.0 11 0.00928332714444857 0.0 0.0 0.0 0.0 12 0.00928332714444857 0.0 0.0 0.0 0.0 13 0.00928332714444857 0.0 0.0 0.0 0.0 14 0.014440731113586664 0.0 0.0 0.0 0.0 15 0.014440731113586664 0.0 0.0 0.0 0.0 16 0.014440731113586664 0.0 0.0 0.0 0.0 17 0.014440731113586664 0.0 0.0 0.0 0.0 18 0.015472211907414284 0.0 0.0 0.0 0.0 19 0.015472211907414284 0.0 0.0 0.0 0.0 20 0.0165036927012419 0.0 0.0 0.0 0.0 21 0.01753517349506952 0.0 0.0 0.0 0.0 22 0.01856665428889714 0.0 0.0 0.0 0.0 23 0.01959813508272476 0.0 0.0 0.0 0.0 24 0.02062961587655238 0.0 0.0 0.0 0.0 25 0.022692577464207615 0.0 0.0 0.0 0.0 26 0.023724058258035235 0.0 0.0 0.0 0.0 27 0.024755539051862854 0.0 0.0 0.0 0.0 28 0.03919627016544952 0.0 0.0 0.0 0.0 29 0.040227750959277137 0.0 0.0 0.0 0.0 30 0.04847959730989809 0.0 0.0 0.0 0.0 31 0.04951107810372571 0.0 0.0 0.0 0.0 32 0.050542558897553325 0.0 0.0 0.0 0.0 33 0.050542558897553325 0.0 0.0 0.0 0.0 34 0.050542558897553325 0.0 0.0 0.0 0.0 35 0.0763295787432438 0.0 0.0 0.0 0.0 36 0.08045550191855427 0.0 0.0 0.0 0.0 37 0.08045550191855427 0.0 0.0 0.0 0.0 38 0.08045550191855427 0.0 0.0 0.0 0.0 39 0.08251846350620952 0.0 0.0 0.0 0.0 40 0.08458142509386475 0.0 0.0 0.0 0.0 41 0.08458142509386475 0.0 0.0 0.0 0.0 42 0.08870734826917523 0.0 0.0 0.0 0.0 43 0.09077030985683046 0.0 0.0 0.0 0.0 44 0.09077030985683046 0.0 0.0 0.0 0.0 45 0.09077030985683046 0.0 0.0 0.0 0.0 46 0.09283327144448571 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGATC 15 0.007142717 52.10559 27 GCCCACA 15 0.007142717 52.10559 27 ACCATGT 15 0.007142717 52.10559 25 GCTGGGT 40 1.8317223E-9 52.10559 26 GTGAGTG 15 0.007142717 52.10559 27 AGAATCC 20 3.39767E-4 52.10559 27 AACTTGG 15 0.007142717 52.10559 26 GCTGTTA 15 0.007142717 52.10559 27 TATCTTC 15 0.007142717 52.10559 27 CCATATT 15 0.007142717 52.10559 25 GCTTGAT 15 0.007142717 52.10559 26 ATCACTG 15 0.007142717 52.10559 27 GATCACT 15 0.007142717 52.10559 26 TACCACT 15 0.007142717 52.10559 25 GTTATCT 15 0.007142717 52.10559 25 AAGCCCG 15 0.007142717 52.10559 25 TAGATCA 15 0.007142717 52.10559 24 ACAGCTC 15 0.007142717 52.10559 27 TCGGACC 35 3.7731297E-8 52.105587 27 CTTCATT 15 0.0071867066 52.024807 30 >>END_MODULE