FastQCFastQC Report
Tue 31 May 2016
SRR212600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97735
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT28982.965160894254873No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17981.8396684913285926No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC15611.5971760372435666No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13811.4130045531283573No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC9770.9996418887808871No Hit
CACAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8950.9157415460172915No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7730.7909142067836497No Hit
CACAAGGGGGAGTAAGATACTTTCAGTTTTGTTCATTTTTCAGAAGCTACTGTAAAGA7010.7172456131375659No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6130.6272062209034635No Hit
CACAAGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5150.5269350795518494No Hit
CACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4780.48907760781705634No Hit
CACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4170.42666393820023535No Hit
CACAAGGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4140.42359441346498183No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4110.4205248887297283No Hit
CACAAGGGGAGTCAAAACGCCTTGTTAAAAAGAAACGAGCTTTAAAATCATGTTTATA3890.3980150406712028No Hit
CACAAGGGGACCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3870.3959686908477004No Hit
CACAAGGGGGGGTATATTAGCCTGGGCTGGCATTTGTGTTCTCTTAGTGTCTGTATAA3830.3918759912006958No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3210.3284391466721236No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2640.27011817670230726No Hit
CACAAGGGGATGCTAACAAGATACGCTCGGACCCAGTTTGTCAGTCCTTGGAGAGAGG2500.255793727937791No Hit
CACAAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2500.255793727937791No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGTTCTTCAGA2300.2353302297027677No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.22714483040875838No Hit
CACAAGGGGAAGATAAAAGAAAAACAGAAGAGGAAGATAAATCACAATCATTTATGCA2210.2261216554970072No Hit
CACAAGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA2190.22407530567350487No Hit
CACAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2180.22305213076175373No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC2130.21793625620299792No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2060.21077403182073973No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA2010.20565815726198391No Hit
CACAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1990.2036118074384816No Hit
CACAAGGGGAGCAGTTTCCTCAACCAGCGCTGTCCCACCGGTGGCTCCGTGGTTTCTC1920.19644958305622345No Hit
CACAAGGGGGGGGCTGGTGAGATGACTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1910.19542640814447232No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1860.19031053358571648No Hit
CACAAGGGGACAGGGCAGGGTGTTGGGTAGGGTTTCTAAGCTATAGGTGTGTTACCCT1850.18928735867396532No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1830.187241008850463No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1810.18519465902696067No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1800.1841714841152095No Hit
CACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1790.18314830920345834No Hit
CACAAGGGGACGTCTGGCGGGGAAACTCACCGCCTCAGCAGCTCAGCTAAAACCCTGA1720.17598608482120018No Hit
CACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1680.17189338517419553No Hit
CACAAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1680.17189338517419553No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1660.1698470353506932No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA1600.1637079858801862No Hit
CACAAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1600.1637079858801862No Hit
CACAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1560.15961528623318155No Hit
CACAAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1540.15756893640967923No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCCCTTCCGA1500.15347623676267458No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1490.15245306185092342No Hit
CACAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1330.1360822632629048No Hit
CACAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1320.13505908835115363No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCCAA1260.12892003888064665No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA1240.1268736890571443No Hit
CACAAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1210.12380416432189083No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA1180.12073463958663733No Hit
CACAAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGAA1160.11868828976313502No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGA1160.11868828976313502No Hit
CACAAGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1150.11766511485138385No Hit
CACAAGGGGTGCCCTCCGTCCGATATGCCCACACTCCAGCTGCTACTGGTTCCCTCCT1110.11357241520437919No Hit
CACAAGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCTAAG1100.11254924029262803No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1100.11254924029262803No Hit
CACAAGGGGGGGCTGGAGAGATGGCTTGGCGGTTAAGAGCACTGGCTGCTCTTCCGAA1090.11152606538087687No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1090.11152606538087687No Hit
CACAAGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1080.11050289046912569No Hit
CACAAGGGGGCAGAGTGCAGTGCTCACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGT1050.10743336573387222No Hit
CACAAGGGGGAAAAAAATGAGAACAAGCTGTTAGTGAAAAAAATGAAACACTGTCTCT1040.10641019082212105No Hit
CACAAGGGGGGTCTTGAGTTCAAATCCCAACAACCACATGGTGGCTTAGAACCTTCTA1040.10641019082212105No Hit
CACAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1040.10641019082212105No Hit
CACAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1020.10436384099861871No Hit
CACAAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1010.10334066608686755No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG1010.10334066608686755No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC980.10027114135161405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA150.00697735452.4152039
GGATGCG150.00697735452.4152039
GGGTATA550.052.41520310
GACCATA150.00697735452.4152039
GGGACTA458.367351E-1152.4152037
AGCATGT150.00697735452.41520310
GGAACAT353.6010533E-852.4152038
GGAGTCC307.4957825E-752.4152038
GCCTATG150.00697735452.4152039
GGGATAC150.00697735452.4152037
GACGTCT251.5682743E-552.41529
GGGTAGC203.2997585E-452.41528
GGACTAA401.7371349E-952.41528
ATGCTAA203.2997585E-452.415210
AAGCATG401.7371349E-952.415210
GGACGTC251.5682743E-552.41528
AGGGGTA503.6379788E-1252.41525
ATGCCAC401.7371349E-952.415210
GGTAGCC203.2997585E-452.41529
GACTAAT401.7371349E-952.41529
Produced by FastQC (version 0.11.5)