FastQCFastQC Report
Tue 31 May 2016
SRR212599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99006
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC24362.460456942003515No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC16501.6665656626871097No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16311.6473749065713188No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13241.3372926893319597No Hit
CACAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11731.1847766802011999No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA10131.0231703129103287No Hit
CACAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8750.8837848211219521No Hit
CACAAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC8160.8241924731834435No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7940.8019715976809486No Hit
CACAAGGGGGAGTAAGATACTTTCAGTTTTGTTCATTTTTCAGAAGCTACTGTAAA7390.7464194089247116No Hit
CACAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5190.5242106538997636No Hit
CACAAGGGGAGTCAAAACGCCTTGTTAAAAAGAAACGAGCTTTAAAATCATGTTTA5170.5221905743086277No Hit
CACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4120.4161363957739935No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC3980.40199583863604227No Hit
CACAAGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3870.39088540088479484No Hit
CACAAGGGGACCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3870.39088540088479484No Hit
CACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3830.3868452417025231No Hit
CACAAGGGGGGGTATATTAGCCTGGGCTGGCATTTGTGTTCTCTTAGTGTCTGTAT3460.3494737692665091No Hit
CACAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3360.3393733713108296No Hit
CACAAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3310.3343231723329899No Hit
CACAAGGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3130.3161424560127669No Hit
CACAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3040.30705209785265536No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.2757408641900491No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGTTCTTCA2470.2494798295052825No Hit
CACAAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT2460.2484697897097146No Hit
CACAAGGGGAGGGTTTTTCTGTGTATTTATAGCTGTCCTGGAATTGTTCTGTAGAC2330.2353392723673313No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2300.2323091529806274No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2290.2312991131850595No Hit
CACAAGGGGATGCTAACAAGATACGCTCGGACCCAGTTTGTCAGTCCTTGGAGAGA2290.2312991131850595No Hit
CACAAGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC2260.22826899379835563No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC2210.22321879482051593No Hit
CACAAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2190.22119871522938003No Hit
CACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2190.22119871522938003No Hit
CACAAGGGGGGGGCTGGTGAGATGACTCAGTGGGTAAGAGCACCCGACTGCTCTTC2160.2181685958426762No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTT2060.20806819788699674No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2020.20402803870472494No Hit
CACAAGGGGACAGGGCAGGGTGTTGGGTAGGGTTTCTAAGCTATAGGTGTGTTACC2000.20200795911358907No Hit
CACAAGGGGAAGATAAAAGAAAAACAGAAGAGGAAGATAAATCACAATCATTTATG1970.19897783972688526No Hit
CACAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1920.19392764074904553No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1900.19190756115790963No Hit
CACAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1880.18988748156677374No Hit
CACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1830.184837282588934No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1730.17473688463325454No Hit
CACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1670.16867664585984687No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1670.16867664585984687No Hit
CACAAGGGGAGTGCAGTGCTCACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAG1630.1646364866775751No Hit
CACAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1630.1646364866775751No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCC1620.16362644688200714No Hit
CACAAGGGGAGCAGTTTCCTCAACCAGCGCTGTCCCACCGGTGGCTCCGTGGTTTC1510.15251600913075974No Hit
CACAAGGGGACGTCTGGCGGGGAAACTCACCGCCTCAGCAGCTCAGCTAAAACCCT1510.15251600913075974No Hit
CACAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCG1470.14847584994848798No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCCCTTCC1400.14140557137951235No Hit
CACAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1380.13938549178837645No Hit
CACAAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTG1360.13736541219724058No Hit
CACAAGGGGATTGTAAATGTATACGTTGGAACTAGACTCCACAACTATACATTTTG1300.1313051734238329No Hit
CACAAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1280.129285093832697No Hit
CACAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1280.129285093832697No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC1270.12827505403712908No Hit
CACAAGGGGGCAGAGTGCAGTGCTCACAAGGGGGCTTTTCCTGTGGGAGCAGCCGG1230.1242348948548573No Hit
CACAAGGGGGGTCTTGAGTTCAAATCCCAACAACCACATGGTGGCTTAGAACCTTC1210.1222148152637214No Hit
CACAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA1160.11716461628588166No Hit
CACAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1150.1161545764903137No Hit
CACAAGGGGAGTGGAGTTTACCCAGGAGAATCCCTTTTGCCGGTTTAAGGCTGTAG1140.11514453669474578No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTCT1120.11312445710360988No Hit
CACAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1110.11211441730804193No Hit
CACAAGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA1100.111104377512474No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT1100.111104377512474No Hit
CACAAGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC1090.11009433771690603No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC1090.11009433771690603No Hit
CACAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1090.11009433771690603No Hit
CACAAGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA1080.1090842979213381No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1070.10807425812577015No Hit
CACAAGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCTA1040.10504413873906632No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA1030.10403409894349837No Hit
CACAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1020.10302405914793043No Hit
CACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.10201401935236247No Hit
CACAAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC1010.10201401935236247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT355.020229E-850.2521750
AACGCAC252.0126297E-550.25216749
ACGCACC252.0126297E-550.25216750
ACTGTAA550.050.25216349
TGGTATG150.00824266250.25216350
GGCCGTG301.002627E-650.25216349
TCGGTGT150.00824266250.25216349
GAAGATT150.00824266250.25216350
CCGTCAT150.00824266250.25216350
CGTGGTT204.0629663E-450.25216348
CCCGTCA150.00824266250.25216349
CCCCGTC150.00824266250.25216348
TCTGTAG301.002627E-650.25216348
TACGTGG150.00824266250.25216349
TATCTCA550.050.25216348
ACCCTTA150.00824266250.25216348
GTCCATG150.00824266250.25216350
TGAAGAT150.00824266250.25216349
CATAGAA150.00824266250.25216348
CGTAAAG150.00824266250.25216349
Produced by FastQC (version 0.11.5)