FastQCFastQC Report
Tue 31 May 2016
SRR212597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250501
Sequences flagged as poor quality0
Sequence length58
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT72732.903381623227053No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT35471.4159624113276992No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC26201.045904008367232No Hit
AGTTTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC25581.021153608169229No Hit
AGTTTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG21310.8506952068055617No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGTGG17570.7013944056111552No Hit
AGTTTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17210.6870232054961857No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.4566848036534784No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG10510.4195592033564737No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9910.3956072031648576No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9530.3804376030435008No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7630.30458960243671684No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.2463064019704512No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC6000.23952000191616005No Hit
AGTTTAGGGGGTAATCTTGTGGTAACATGTAGATTTTTATCAGAATAATCTCTGAAAT5980.23872160190977285No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5740.2291408018331264No Hit
AGTTTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5650.225548001804384No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.21916080175328642No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGA5480.21876160175009282No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.20319280162554243No Hit
AGTTTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCAACCGACTGCTCTTCCA4820.19241440153931522No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC4750.18962000151696No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGTTCTTCCGA4510.1800392014403136No Hit
AGTTTAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4430.17684560141476482No Hit
AGTTTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4200.167664001341312No Hit
AGTTTAGGGGAGTGAAAAAGAGAGAGGAGAGGAGGAGGAAGCCAACTTTGTGCTAAGG4130.1648696013189568No Hit
AGTTTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA4010.1600792012806336No Hit
AGTTTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3900.155688001245504No Hit
AGTTTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC3860.1540912012327296No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC3680.14690560117524482Illumina Single End Adapter 2 (96% over 25bp)
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA3640.1453088011624704No Hit
AGTTTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT3540.1413168011305344No Hit
AGTTTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC3370.1345304010762432No Hit
AGTTTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG3340.1333328010666624No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG3270.13053840104430722No Hit
AGTTTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3180.1269456010155648No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGG3140.12534880100279042No Hit
AGTTTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3110.12415120099320961No Hit
AGTTTAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3080.12295360098362881No Hit
AGTTTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3040.1213568009708544No Hit
AGTTTAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC2940.1173648009389184No Hit
AGTTTAGGGAAGGGTGTTTCTCTCGAACATGCCATCTGGTGCCAAATGAAAATTCTTA2940.1173648009389184No Hit
AGTTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2870.1145704009165632No Hit
AGTTTAGGGCATTGACTGTTTGTGGTCAGGTGTCCAGGGAAAAAAGTTATTTTAGGTT2870.1145704009165632No Hit
AGTTTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2830.11297360090378882No Hit
AGTTTAGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTCCG2770.11057840088462721No Hit
AGTTTAGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2710.1081832008654656No Hit
AGTTTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2700.107784000862272No Hit
AGTTTAGGGGAACTCCAGTGTTTCAGAGACTCAGTCTCTACAGCGGGGAACGGGTACA2680.1069856008558848No Hit
AGTTTAGGGGCACGTGCTGCTCCGGCTCCCGCGTGGCGTTCTCAGCATCCAGTCTTCT2670.10658640085269122No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT2660.10618720084949762No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCACCGACTGCTCTTCCAA2650.10578800084630402No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC2630.1049896008399168No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.103792000830336No Hit
AGTTTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2590.1033928008271424No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2560.1021952008175616No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT2550.101796000814368Illumina Single End Adapter 2 (96% over 32bp)
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGA2530.1009976008079808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAT150.007182291252.0571852
TATGGAT353.833702E-852.0571852
CGCGGGC203.4238235E-452.05717552
GTAGATG203.4238235E-452.05717552
TATATGG203.4272048E-452.04677651
ACAATCA203.4272048E-452.04677651
TATTACT203.4272048E-452.04677650
TTGTTAC203.4272048E-452.04677649
GGCGTTT150.007187972352.04677251
GCGGATG150.007187972352.04677249
CGGACTC353.8397047E-852.04677250
AGATCCT251.6426846E-552.0467750
TAAACTC251.6426846E-552.0467749
ACGGGTA251.6426846E-552.0467750
TATCGGG251.6426846E-552.0467750
CACTAAC459.094947E-1152.02597840
TGTTTGG307.9424535E-752.02597441
AACCGAC503.6379788E-1252.02597442
CGAACCT150.007199343852.02597447
TACTTCT150.007199343852.02597447
Produced by FastQC (version 0.11.5)