FastQCFastQC Report
Tue 31 May 2016
SRR212596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248899
Sequences flagged as poor quality0
Sequence length58
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT71642.8782759271833154No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT33771.3567752381488072No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC26391.0602694265545463No Hit
AGTTTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC25611.0289314139470227No Hit
AGTTTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG20920.8405015689094772No Hit
AGTTTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17680.7103282857705334No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGTGG14450.5805567720239937No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.421054323239547No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9790.3933322351636607No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG9600.38569861670798194No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC8780.3527535265308418No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7340.2948987340246445No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGA5940.23865101908806383No Hit
AGTTTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5830.2342315557716182No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC5750.23101740063238502No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.2274014761007477No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.22378555156911034No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5570.22378555156911034No Hit
AGTTTAGGGGGTAATCTTGTGGTAACATGTAGATTTTTATCAGAATAATCTCTGAAAT5390.21655370250583567No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.206509467695732No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGTTCTTCCGA4670.18762630625273707No Hit
AGTTTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCAACCGACTGCTCTTCCA4620.18561745929071632No Hit
AGTTTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4530.182001534759079No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC4500.18079622658186656No Hit
AGTTTAGGGGAGTGAAAAAGAGAGAGGAGAGGAGGAGGAAGCCAACTTTGTGCTAAGG4270.17155553055657113No Hit
AGTTTAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4030.16191306513887158No Hit
AGTTTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC3920.15749360182242597No Hit
AGTTTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA3860.15508298546800106No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC3720.149458213974343Illumina Single End Adapter 2 (96% over 25bp)
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA3640.14624405883510982No Hit
AGTTTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3600.14463698126549324No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG3580.14383344248068494No Hit
AGTTTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT3370.1353962852401978No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGG3330.13378920767058122No Hit
AGTTTAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC3210.12896797496173146No Hit
AGTTTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC3060.12294143407566925No Hit
AGTTTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3050.12253966468326509No Hit
AGTTTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2950.11852197075922363No Hit
AGTTTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2920.11731666258201116No Hit
AGTTTAGGGAAGGGTGTTTCTCTCGAACATGCCATCTGGTGCCAAATGAAAATTCTTA2860.1149060462275863No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2830.11370073805037385No Hit
AGTTTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2810.11289719926556555No Hit
AGTTTAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2810.11289719926556555No Hit
AGTTTAGGGCATTGACTGTTTGTGGTCAGGTGTCCAGGGAAAAAAGTTATTTTAGGTT2740.11008481351873652No Hit
AGTTTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2720.10928127473392821No Hit
AGTTTAGGGGAACTCCAGTGTTTCAGAGACTCAGTCTCTACAGCGGGGAACGGGTACA2690.10807596655671578No Hit
AGTTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2660.10687065837950334No Hit
AGTTTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2620.10526358080988675No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCACCGACTGCTCTTCCAA2600.10446004202507844No Hit
AGTTTAGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTCCG2540.10204942567065356No Hit
AGTTTAGGGGTTAAGGGTGTACCAGCTCACTAAGAAACACTGCTATCAAGATTTTTAA2540.10204942567065356No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA2540.10204942567065356No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT2520.10124588688584525Illumina Single End Adapter 2 (96% over 32bp)
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT2500.10044234810103697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA150.007138350552.13790524
GTACAGC150.007138350552.13790524
TAAGTCA150.007138350552.13790524
CCTAATG307.825256E-752.13790523
ATCACGT150.007138350552.13790524
TTAAGTC150.007138350552.13790523
ACGTGTA150.007138350552.13790527
GATATAA150.007138350552.13790525
ACGCTTC150.007138350552.13790524
CGTACGT150.007138350552.13790523
CGTCGTG150.007138350552.13790525
TACAGCC150.007138350552.13790525
GTACGTC150.007138350552.13790524
TACGTCC150.007138350552.13790525
TCACGTG150.007138350552.13790525
ACGCGAT401.8408173E-952.137924
ATGTAGA850.052.137927
CTAAAGG203.3976804E-452.137923
TAGTGTG203.3976804E-452.137926
AATACCC251.6257241E-552.137927
Produced by FastQC (version 0.11.5)