##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212596_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 248899 Sequences flagged as poor quality 0 Sequence length 58 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.161165774069 38.0 37.0 40.0 33.0 40.0 2 35.40199036556997 38.0 34.0 39.0 27.0 40.0 3 37.118208590633145 38.0 37.0 40.0 33.0 40.0 4 34.31358502846536 37.0 32.0 39.0 24.0 40.0 5 34.59122374939233 38.0 33.0 39.0 24.0 40.0 6 36.55232845451368 38.0 36.0 40.0 31.0 40.0 7 36.078003527535266 38.0 35.0 40.0 29.0 40.0 8 35.48595615088851 38.0 35.0 40.0 25.0 40.0 9 35.40332022225883 38.0 35.0 40.0 25.0 40.0 10 36.07800754522919 38.0 35.0 39.0 30.0 40.0 11 36.79788588945717 39.0 36.0 40.0 31.0 40.0 12 36.64115564948031 39.0 35.0 40.0 31.0 40.0 13 36.492946938316344 38.0 35.0 40.0 31.0 40.0 14 36.50365007492999 38.0 35.0 40.0 31.0 40.0 15 36.486088734788005 38.0 35.0 40.0 31.0 40.0 16 36.41512018931374 38.0 36.0 40.0 31.0 40.0 17 36.2740027079257 38.0 35.0 39.0 30.0 40.0 18 36.10685056990988 38.0 35.0 39.0 30.0 40.0 19 35.93111663767231 38.0 35.0 39.0 30.0 40.0 20 35.9188988304493 38.0 35.0 39.0 30.0 40.0 21 35.978280346646635 38.0 35.0 39.0 30.0 40.0 22 35.765274267875725 38.0 35.0 39.0 30.0 40.0 23 35.476277526225495 38.0 35.0 39.0 29.0 40.0 24 35.250286260692086 38.0 35.0 39.0 28.0 40.0 25 35.409531577065394 38.0 35.0 39.0 29.0 40.0 26 35.01511858223617 38.0 35.0 39.0 28.0 40.0 27 34.52592417004488 38.0 34.0 39.0 26.0 40.0 28 34.32115838151218 38.0 33.0 39.0 25.0 40.0 29 34.4147626145545 38.0 33.0 39.0 26.0 40.0 30 33.905724812072364 38.0 33.0 39.0 23.0 40.0 31 33.36170093089968 38.0 33.0 39.0 20.0 40.0 32 32.924403071125234 38.0 32.0 39.0 18.0 40.0 33 32.88373195553216 38.0 32.0 39.0 18.0 40.0 34 32.376923973177874 37.0 31.0 39.0 17.0 40.0 35 32.04982744004596 36.0 31.0 39.0 14.0 40.0 36 32.57504047826628 38.0 33.0 39.0 2.0 40.0 37 32.34047545389897 38.0 33.0 39.0 2.0 40.0 38 32.25828950698878 38.0 33.0 39.0 2.0 40.0 39 32.24002105271616 38.0 33.0 39.0 2.0 40.0 40 32.07913651722185 38.0 32.0 39.0 2.0 40.0 41 32.222021783936455 38.0 33.0 39.0 2.0 40.0 42 31.99008834908939 38.0 32.0 39.0 2.0 40.0 43 31.93133761083813 38.0 32.0 39.0 2.0 40.0 44 31.841019851425678 38.0 32.0 39.0 2.0 40.0 45 31.688407747720962 38.0 32.0 39.0 2.0 40.0 46 31.26553742682775 38.0 31.0 39.0 2.0 40.0 47 31.166103519901647 38.0 31.0 39.0 2.0 40.0 48 31.18403047019072 38.0 31.0 39.0 2.0 40.0 49 30.984339029084087 37.0 31.0 39.0 2.0 40.0 50 30.798207304971093 37.0 30.0 39.0 2.0 40.0 51 30.52008244307932 37.0 30.0 39.0 2.0 40.0 52 30.41868388382436 37.0 30.0 39.0 2.0 40.0 53 30.16401833675507 36.0 29.0 39.0 2.0 40.0 54 29.929401082366745 36.0 29.0 39.0 2.0 40.0 55 29.625679492484903 36.0 28.0 39.0 2.0 40.0 56 29.376064186678132 37.0 28.0 39.0 2.0 40.0 57 29.066251772807444 36.0 27.0 39.0 2.0 40.0 58 28.770191925238752 36.0 26.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.006414246945738 1 2 -3.81601359347027 1 3 -1.3144609233055533 1 4 -4.082156207221274 1 5 -4.009727260206329 1 6 -2.583574988359203 1 7 -2.943472215362789 1 8 -2.782335644577735 1 9 -3.3732905687906296 1 10 -2.7931568860624907 1 11 -2.8932711862374845 1 12 -2.2527842085693806 1 13 -2.557897966263063 1 14 -2.265765354650746 1 15 -2.3061600501087867 1 16 -1.888242593115507 1 17 -2.291434853336149 1 18 -2.1868329590810163 1 19 -2.008178801765645 1 20 -1.764275077894041 1 21 -1.705307981386433 1 22 -1.7483082947993864 1 23 -1.5271768294048798 1 24 -1.6984913571816378 1 25 -1.47332296241283 1 26 -1.48322240233243 1 27 -1.173704584114624 1 28 -1.5943409912623352 1 29 -1.8598829493863676 1 30 -1.9747366348996422 1 31 -1.66687535093196 1 32 -1.491215683666553 1 33 -1.753119642024597 1 34 -1.7449152719031815 1 35 -1.717042355893252 1 36 -1.9775806864781522 1 37 -1.5737134497725656 1 38 -1.2229094065570543 1 39 -1.7827797043616656 1 40 -1.3192396864044404 1 41 -0.7853444643940577 1 42 -1.3362606426711636 1 43 -0.8164667243346635 1 44 -0.9265696140225721 1 45 -0.8887753024213438 1 46 -0.6198202260704626 1 47 -0.7708558693155041 1 48 -0.44649474805174094 1 49 -0.5499368337419739 1 50 -0.30630270912383395 1 51 -0.7132008293899403 1 52 -0.5162242793732936 1 53 -0.20434284662560032 1 54 -0.23919063405541507 1 55 -0.880841667073021 1 56 -1.0828751906355336 1 57 -0.8456062329453538 1 58 -1.2416633992599344 2 1 -1.2189597892836517 2 2 -3.429046383953569 2 3 -1.4445693469919618 2 4 -5.148461461599922 2 5 -3.6221674515938886 2 6 -2.785212317712613 2 7 -2.597372395337324 2 8 -3.4086909406577988 2 9 -3.366486653246973 2 10 -2.3466883377107948 2 11 -1.1647474164293072 2 12 -1.9153758419480695 2 13 -1.6566425341046838 2 14 -1.7584785805846366 2 15 -1.2179286291878455 2 16 -0.9241277605796228 2 17 -0.985083068954431 2 18 -1.6256196673027787 2 19 -1.4230420650113302 2 20 -1.423157479895707 2 21 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3 4 -5.161335092851772 3 5 -3.9309232119625506 3 6 -2.1963018201013114 3 7 -2.5857671021398474 3 8 -2.1455530723112943 3 9 -3.0943013067793004 3 10 -1.89165092173819 3 11 -1.668721542332996 3 12 -1.8521491899377551 3 13 -1.371625834320298 3 14 -1.8194222240970888 3 15 -1.5069405938434883 3 16 -1.7835747651879146 3 17 -1.7900126761924113 3 18 -1.719984018547862 3 19 -1.1625677065789972 3 20 -1.6327292044126835 3 21 -1.6937321209141416 3 22 -1.2770054041416614 3 23 -1.683153280797292 3 24 -1.7483667788737023 3 25 -1.556448665232928 3 26 -1.2057324898679838 3 27 -0.9777748771492583 3 28 -0.8503108278773368 3 29 -0.8932655260626632 3 30 -1.2776493418870487 3 31 -1.099217202628651 3 32 -1.0430645139534143 3 33 -1.5600783878627986 3 34 -1.5859842199330778 3 35 -1.676736312191622 3 36 -0.5924903947664717 3 37 -0.670333298514656 3 38 -0.5633719999907854 3 39 -0.26336533265527606 3 40 -0.19342662001412236 3 41 -0.23757474684505553 3 42 -0.17785877289407992 3 43 -0.5645760004575244 3 44 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4 26 -0.16001223043804913 4 27 0.2427405691726392 4 28 0.4208105238891804 4 29 0.12813317678765657 4 30 0.5680154473261254 4 31 0.3049259958693895 4 32 1.1463662762838283 4 33 1.186828080817861 4 34 0.7234969201932202 4 35 0.9966294498838195 4 36 0.6300305132383102 4 37 1.1249175975426908 4 38 1.3707464502566964 4 39 1.1767054985445924 4 40 1.2415878875810265 4 41 0.924760975974543 4 42 1.3631678528099656 4 43 1.3494024941661351 4 44 1.123736074441009 4 45 0.9214320341017803 4 46 1.189323846231506 4 47 1.4313858460841047 4 48 1.7668004849803332 4 49 1.0158747912801758 4 50 1.7209212852054243 4 51 1.4241596171810293 4 52 0.9941203954450657 4 53 0.9930255744270298 4 54 1.4949490079789634 4 55 1.8881641808801959 4 56 1.655030178848783 4 57 1.93722204988293 4 58 1.2215210770824392 5 1 -0.9127304733773158 5 2 -2.55696310298503 5 3 -1.1646062118070049 5 4 -3.738445123784164 5 5 -2.5856377712285905 5 6 -2.6290186098662716 5 7 -2.1568169096014955 5 8 -2.5872339426267175 5 9 -2.617921448605358 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-0.9869796770904316 117 38 -0.9131459326922879 117 39 -1.1913063812597429 117 40 -1.0386357322652309 117 41 -0.858694649462219 117 42 -0.954704264590454 117 43 -0.6254402731294633 117 44 -0.8702366718902255 117 45 -0.5565553872818647 117 46 -0.2577411432863137 117 47 -0.6879363062569119 117 48 -0.26508281341239837 117 49 -0.20853121850319312 117 50 -0.3476496442145631 117 51 -0.39109195514601325 117 52 -0.4874484375388626 117 53 0.16283689518174782 117 54 -0.02374421354444678 117 55 -0.25124881702336666 117 56 0.1288247281150312 117 57 0.17883630564812592 117 58 0.1701583936442823 118 1 -0.6524974533310441 118 2 -2.536978255430917 118 3 -0.42860686704791107 118 4 -3.5432242871234365 118 5 -2.9450374067508385 118 6 -1.381480882323629 118 7 -1.9722232613430037 118 8 -1.8907742911329706 118 9 -2.511424325522455 118 10 -0.9014667965909524 118 11 -0.770971960799244 118 12 -1.0839898214601433 118 13 -0.8505578671363239 118 14 -0.7534388800868399 118 15 -0.9462784872534229 118 16 -0.5810784332253647 118 17 -0.8220847557113302 118 18 -0.8032639672986619 118 19 -1.259879190119321 118 20 -1.3945237529857621 118 21 -1.2808867571068205 118 22 -1.3100694036456986 118 23 -1.5663340375495807 118 24 -1.7722141885159886 118 25 -1.0162242433362962 118 26 -0.7539542666446621 118 27 -0.026151641423609817 118 28 -0.23315061213473598 118 29 -0.3091073148250345 118 30 -0.8201675787492775 118 31 -0.16096422170244296 118 32 -0.7762587029555021 118 33 -0.6273016318544506 118 34 -0.6895158683801021 118 35 -0.8546826245224288 118 36 -0.5852576448749645 118 37 -0.9602219141663255 118 38 -1.2970828139851243 118 39 -0.8029698045612079 118 40 -0.7126140722263123 118 41 -0.7998487234109426 118 42 -0.7795202388691571 118 43 -1.1453658167456773 118 44 -0.8425693565505199 118 45 -0.7586875473630919 118 46 -0.9178012160505098 118 47 -0.607768779393389 118 48 -1.0557165387698717 118 49 -0.7497995153134127 118 50 -1.7692589174187248 118 51 -1.5636953902928106 118 52 -1.3975296626848142 118 53 -1.1493511071873215 118 54 -0.8528752737018195 118 55 -1.2610635221591657 118 56 -0.2228970757600841 118 57 -0.6429971828026737 118 58 -0.28607732849803114 119 1 -0.6871470132048145 119 2 -2.7252152592508807 119 3 -0.5909760748207091 119 4 -2.864142739207807 119 5 -2.237908192334629 119 6 -1.588049558623247 119 7 -1.4166469451164616 119 8 -0.981830594072683 119 9 -1.1158920219183983 119 10 -1.0435113074695437 119 11 -1.2468065397728338 119 12 -0.8253741430014188 119 13 -0.9126737947230978 119 14 -1.0475968272716898 119 15 -0.9977026692829867 119 16 -0.6046833161351799 119 17 -0.7181360760900048 119 18 -0.6578147063785522 119 19 -0.8441259929759894 119 20 -0.5109080745270376 119 21 -0.22464609940876556 119 22 -0.4132914597825561 119 23 -0.08211214749809415 119 24 -0.3846955039121056 119 25 -0.5660706143710073 119 26 -1.2016788971047205 119 27 -0.05818535675329173 119 28 -0.35233762425897197 119 29 -0.399644600990122 119 30 0.06801544732612541 119 31 -0.4172962263528319 119 32 -0.3258559459383932 119 33 -0.5724311784413985 119 34 -0.1515030798067798 119 35 -0.29966684641248165 119 36 -0.013488005280208881 119 37 -0.2269342543091568 119 38 -0.3653646608544143 119 39 -0.1936648718257814 119 40 0.12584714684028597 119 41 0.2210572722708335 119 42 0.2983530379951489 119 43 0.24755064231428037 119 44 0.517254592959528 119 45 0.7177283303980779 119 46 0.32821273512039184 119 47 0.2276821423804023 119 48 0.4843930775729284 119 49 0.20568960609499243 119 50 0.0727731370572755 119 51 0.0491596171810329 119 52 -0.15865738233271287 119 53 0.1920996485011024 119 54 0.42550456353451693 119 55 0.13353455125056612 119 56 0.36336351218211504 119 57 0.10851834617922407 119 58 0.7122618178231797 120 1 -1.8231069527361825 120 2 -6.121379280414903 120 3 -2.0816223333241055 120 4 -5.931414092193446 120 5 -4.443596023022462 120 6 -3.925729074874191 120 7 -4.656537345721151 120 8 -5.1269553106259735 120 9 -5.486829285712808 120 10 -3.4061341343780853 120 11 -2.986035564716147 120 12 -2.900865450635578 120 13 -3.173633734254466 120 14 -3.1867691628726007 120 15 -3.08396495197384 120 16 -2.5822910258857448 120 17 -2.5806644207612024 120 18 -2.1230452430376587 120 19 -2.721488048909478 120 20 -2.578746320936709 120 21 -2.664271949748901 120 22 -2.63003371223909 120 23 -3.0131401142403433 120 24 -2.607484619558363 120 25 -3.0597403044692726 120 26 -2.872124853264957 120 27 -2.624417405128195 120 28 -2.4380330135257893 120 29 -2.683752688669639 120 30 -3.0841009547902765 120 31 -4.026801139429175 120 32 -4.327462143973165 120 33 -4.335753173906248 120 34 -4.377788038234591 120 35 -4.358434798037575 120 36 -2.7182310135946466 120 37 -2.3424860305752198 120 38 -2.5931613350796603 120 39 -2.9870322187645577 120 40 -2.2841112809752815 120 41 -2.1847439371018 120 42 -2.0089976800698537 120 43 -2.034951247330465 120 44 -1.8232593904115966 120 45 -1.7809489182791687 120 46 -1.9223541583036443 120 47 -2.474131916576514 120 48 -1.6964459246946468 120 49 -1.738565873874773 120 50 -1.8591051698210315 120 51 -2.163425416832574 120 52 -2.0949763543659685 120 53 -1.6651376908790922 120 54 -2.126555149239479 120 55 -1.5341714200268335 120 56 -1.1907748098133482 120 57 -1.4193539138358915 120 58 -1.4491138435524817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 769.0 3 107.0 4 97.0 5 90.0 6 200.0 7 392.0 8 430.0 9 648.0 10 769.0 11 858.0 12 1177.0 13 1449.0 14 1565.0 15 1759.0 16 1899.0 17 2092.0 18 2452.0 19 2575.0 20 2630.0 21 2461.0 22 3054.0 23 2801.0 24 2899.0 25 2637.0 26 2770.0 27 3178.0 28 3818.0 29 4805.0 30 5782.0 31 6873.0 32 8535.0 33 10572.0 34 12973.0 35 17315.0 36 23799.0 37 34810.0 38 42019.0 39 35784.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 44.30753036372183 38.68717833338021 6.293315762618572 10.71197554027939 11 26.400266774876556 36.387852100651266 14.03902787877814 23.172853245694036 12 45.54377478414939 14.229064801385302 22.98402163126409 17.243138783201218 13 20.336763104713157 16.28853470684896 28.695173544289048 34.67952864414883 14 19.734109016106935 31.98204894354738 30.07766202355172 18.20618001679396 15 33.884828785973426 15.872301616318266 32.715278084684954 17.527591513023356 16 22.136689982683738 16.649725390620294 38.978461142873215 22.235123483822754 17 36.82055773627054 16.329515184874186 19.963921108562108 26.88600597029317 18 34.51267687139074 18.009234886814365 24.79796173429406 22.680126507500834 19 23.35109203992093 19.295115953907718 36.70949648843675 20.6442955177346 20 22.958308390150222 32.778355879292405 23.618415501870235 20.644920228687138 21 24.72980899806346 18.129514901686637 35.77569928243698 21.36497681781292 22 28.102161921100528 19.50309161547455 20.902052639825794 31.492693823599133 23 29.40349298309756 30.75183106400588 23.09772236931446 16.746953583582094 24 24.679160070395938 32.76263872258697 23.878366107088613 18.67983509992848 25 24.983025106771073 20.135236664162285 23.144915366597427 31.736822862469218 26 33.40900415854712 17.48679108825361 25.913976334451654 23.190228418747612 27 18.712477133716384 19.428483935176605 26.419724234634263 35.43931469647275 28 21.753769873955825 31.799936515844923 27.0458572570827 19.40043635311655 29 33.554603682302925 18.069563540218432 29.24344828140445 19.13238449607419 30 19.181232011062693 27.10922802334743 33.196925600167226 20.51261436542265 31 32.403698182651006 16.76798765665243 34.308639941497674 16.519674219198887 32 27.050313454428547 15.081578524352999 42.66074586079409 15.20736216042437 33 32.159364022924755 14.058292539808853 33.82727660019131 19.955066837075083 34 25.047420028934255 15.550956437871724 39.671274714676095 19.730348818517925 35 20.054979282295303 28.592441956265752 39.94156441779432 11.411014343644627 36 25.79281013960855 22.251745609345292 36.04617975856927 15.909264492476897 37 21.015267605180856 21.419268216206653 43.5034289803105 14.062035198301992 38 25.329050517395256 19.541620769781222 40.079438463332075 15.04989024949145 39 21.661091096242924 20.183832990221305 38.94871011322697 19.2063658003088 40 22.319016530520358 19.817328101884605 43.31261658197723 14.551038785617804 41 20.775200032165976 20.949298379638936 41.92392746572313 16.351574122471956 42 23.52877320371018 19.723706482363774 40.37656651428709 16.370953799638954 43 19.502771981876585 17.57786273965884 44.181250226140655 18.738115052323923 44 16.853553120916676 16.835862900794048 46.65232686702181 19.658257111267464 45 19.382902168887817 17.761965064624416 45.450963838469114 17.404168928018652 46 19.53366250643335 22.057144235717963 42.358787956767884 16.050405301080804 47 21.680689843787707 19.20639006419022 43.204523882301835 15.908396209720232 48 24.86858457442316 18.60930907862403 40.844758867273434 15.677347479679375 49 22.46829460575906 18.76138799840719 41.314552102229534 17.45576529360422 50 15.037651450546269 20.733640122930378 43.525639994207474 20.703068432315884 51 15.146212943968465 21.57113551345481 44.749205583041714 18.533445959535015 52 19.143647742080795 19.55066844704709 45.68204121687929 15.623642593992827 53 14.008888531381341 19.579302994349145 46.24690811832606 20.164900355943452 54 14.978416629453958 22.560153839345702 44.069098969710865 18.392330561489473 55 18.596958588025096 19.656503140807153 42.88197727995235 18.8645609912154 56 16.635872168616984 21.51066133700452 40.893885782602915 20.959580711775576 57 19.73137668442999 20.68568576626471 40.361116030460614 19.22182151884469 58 20.20083715688642 22.103356677131128 39.39265877807293 18.303147387909522 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 71.5 7 143.0 8 220.0 9 354.0 10 411.0 11 568.0 12 725.0 13 1553.0 14 2381.0 15 2995.0 16 3712.5 17 3816.0 18 4376.5 19 4937.0 20 5855.0 21 6773.0 22 7685.0 23 9026.5 24 9456.0 25 9771.0 26 10086.0 27 9370.0 28 7480.5 29 6307.0 30 9602.0 31 12897.0 32 8915.0 33 4933.0 34 3972.5 35 3063.5 36 3115.0 37 3444.5 38 3774.0 39 3755.0 40 3736.0 41 4376.5 42 6239.0 43 7461.0 44 6598.0 45 5735.0 46 6054.5 47 6906.0 48 7438.0 49 10029.5 50 12621.0 51 11990.5 52 11360.0 53 10571.5 54 11247.5 55 12712.0 56 13400.5 57 14089.0 58 13781.5 59 16337.0 60 19200.0 61 14163.5 62 9127.0 63 7591.5 64 6056.0 65 5106.5 66 3321.0 67 2485.0 68 2161.0 69 1837.0 70 1458.0 71 1079.0 72 796.0 73 403.0 74 293.0 75 247.0 76 201.0 77 165.0 78 92.0 79 55.0 80 40.5 81 26.0 82 17.5 83 9.0 84 6.5 85 3.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 1.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.02410616354424887 19 0.002812385746829035 20 0.0 21 4.0176939240414787E-4 22 0.0 23 0.0 24 0.008437157240487105 25 8.035387848082957E-4 26 0.00562477149365807 27 0.28806865435377405 28 0.007231849063274661 29 0.013258389949336879 30 0.054238867974559964 31 0.008035387848082958 32 0.023704394151844724 33 0.034150398354352574 34 0.023704394151844724 35 0.03133801260752354 36 0.053033559797347515 37 0.05504240675936826 38 0.061470717037834625 39 0.07834503151880882 40 0.059060100683409736 41 0.07593441516438394 42 0.07151495184793832 43 0.06428310278466366 44 0.06990787427832174 45 0.06187248643023878 46 0.07834503151880882 47 0.1056653502022909 48 0.10446004202507844 49 0.1132989686579697 50 0.12173612589845681 51 0.11611135440479872 52 0.1056653502022909 53 0.10606711959469503 54 0.1317803607085605 55 0.15910067939204256 56 0.19204576956918268 57 0.17959091840465408 58 0.1763767632654209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 248899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.76269089068257 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39959540667579 24.44405160326076 2 10.824061402986851 7.308988786616258 3 4.865829713809722 4.928505136621681 4 2.770274290474207 3.741276582067425 5 1.804010233831142 3.0454119944234406 6 1.2066400904385077 2.4443649833868357 7 0.9555542333551496 2.2583457547037153 8 0.7532575712500744 2.0345602031346046 9 0.6592491223894806 2.0032221905270813 >10 3.3533646694829535 19.673441837853908 >50 0.22014636758493486 5.143451761557901 >100 0.16421729041470814 10.869067372709413 >500 0.014279764383887667 3.228618837359732 >1k 0.008329862557267805 5.998417028593927 >5k 0.001189980365323972 2.8782759271833154 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 7164 2.8782759271833154 No Hit AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 3377 1.3567752381488072 No Hit AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 2639 1.0602694265545463 No Hit AGTTTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 2561 1.0289314139470227 No Hit AGTTTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 2092 0.8405015689094772 No Hit AGTTTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1768 0.7103282857705334 No Hit AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGTGG 1445 0.5805567720239937 No Hit AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1048 0.421054323239547 No Hit AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 979 0.3933322351636607 No Hit AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 960 0.38569861670798194 No Hit AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 878 0.3527535265308418 No Hit AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 734 0.2948987340246445 No Hit AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGA 594 0.23865101908806383 No Hit AGTTTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 583 0.2342315557716182 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 575 0.23101740063238502 No Hit AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.2274014761007477 No Hit AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 557 0.22378555156911034 No Hit AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 557 0.22378555156911034 No Hit AGTTTAGGGGGTAATCTTGTGGTAACATGTAGATTTTTATCAGAATAATCTCTGAAAT 539 0.21655370250583567 No Hit AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 514 0.206509467695732 No Hit AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGTTCTTCCGA 467 0.18762630625273707 No Hit AGTTTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCAACCGACTGCTCTTCCA 462 0.18561745929071632 No Hit AGTTTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 453 0.182001534759079 No Hit AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 450 0.18079622658186656 No Hit AGTTTAGGGGAGTGAAAAAGAGAGAGGAGAGGAGGAGGAAGCCAACTTTGTGCTAAGG 427 0.17155553055657113 No Hit AGTTTAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 403 0.16191306513887158 No Hit AGTTTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC 392 0.15749360182242597 No Hit AGTTTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA 386 0.15508298546800106 No Hit AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 372 0.149458213974343 Illumina Single End Adapter 2 (96% over 25bp) AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 364 0.14624405883510982 No Hit AGTTTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 360 0.14463698126549324 No Hit AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG 358 0.14383344248068494 No Hit AGTTTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 337 0.1353962852401978 No Hit AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGG 333 0.13378920767058122 No Hit AGTTTAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC 321 0.12896797496173146 No Hit AGTTTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC 306 0.12294143407566925 No Hit AGTTTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 305 0.12253966468326509 No Hit AGTTTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 295 0.11852197075922363 No Hit AGTTTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 292 0.11731666258201116 No Hit AGTTTAGGGAAGGGTGTTTCTCTCGAACATGCCATCTGGTGCCAAATGAAAATTCTTA 286 0.1149060462275863 No Hit AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 283 0.11370073805037385 No Hit AGTTTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 281 0.11289719926556555 No Hit AGTTTAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 281 0.11289719926556555 No Hit AGTTTAGGGCATTGACTGTTTGTGGTCAGGTGTCCAGGGAAAAAAGTTATTTTAGGTT 274 0.11008481351873652 No Hit AGTTTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 272 0.10928127473392821 No Hit AGTTTAGGGGAACTCCAGTGTTTCAGAGACTCAGTCTCTACAGCGGGGAACGGGTACA 269 0.10807596655671578 No Hit AGTTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 266 0.10687065837950334 No Hit AGTTTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 262 0.10526358080988675 No Hit AGTTTAGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCACCGACTGCTCTTCCAA 260 0.10446004202507844 No Hit AGTTTAGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTCCG 254 0.10204942567065356 No Hit AGTTTAGGGGTTAAGGGTGTACCAGCTCACTAAGAAACACTGCTATCAAGATTTTTAA 254 0.10204942567065356 No Hit AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA 254 0.10204942567065356 No Hit AGTTTAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 252 0.10124588688584525 Illumina Single End Adapter 2 (96% over 32bp) AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT 250 0.10044234810103697 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.029329165645502794 0.0 0.0 0.0 0.0 11 0.055444176151772405 0.0 0.0 0.0 0.0 12 0.05584594554417655 0.0 0.0 0.0 0.0 13 0.056649484328984846 0.0 0.0 0.0 0.0 14 0.08798749693650838 0.0 0.0 0.0 0.0 15 0.08879103572131668 0.0 0.0 0.0 0.0 16 0.08919280511372082 0.0 0.0 0.0 0.0 17 0.08959457450612497 0.0 0.0 0.0 0.0 18 0.09079988268333741 0.0 0.0 0.0 0.0 19 0.09441580721497475 0.0 0.0 0.0 0.0 20 0.12615558921490244 0.0 0.0 0.0 0.0 21 0.12816443617692316 0.0 0.0 0.0 0.0 22 0.13137859131615634 0.0 0.0 0.0 0.0 23 0.1426281343034725 0.0 0.0 0.0 0.0 24 0.1655289896705089 0.0 0.0 0.0 0.0 25 0.16673429784772137 0.0 0.0 0.0 0.0 26 0.1679396060249338 0.0 0.0 0.0 0.0 27 0.17075199177176284 0.0 0.0 0.0 0.0 28 0.28565803799934913 0.0 0.0 0.0 0.0 29 0.2912828094930072 0.0 0.0 0.0 0.0 30 0.3350756732650593 0.0 0.0 0.0 0.0 31 0.33949513658150493 0.0 0.0 0.0 0.0 32 0.3407004447587174 0.0 0.0 0.0 0.0 33 0.34311106111314227 0.0 0.0 0.0 0.0 34 0.3455216774675672 0.0 0.0 0.0 0.0 35 0.43431271318888387 0.0 0.0 0.0 0.0 36 0.4387321765053295 0.0 0.0 0.0 0.0 37 0.4407410234673502 0.0 0.0 0.0 0.0 38 0.4439551786065834 0.0 0.0 0.0 0.0 39 0.4471693337458166 0.0 0.0 0.0 0.0 40 0.45279410523947466 0.0 0.0 0.0 0.0 41 0.4584188767331327 0.0 0.0 0.0 0.0 42 0.46203480126477003 0.0 0.0 0.0 0.0 43 0.4664542645812157 0.0 0.0 0.0 0.0 44 0.47047195850525714 0.0 0.0 0.0 0.0 45 0.47569496060651106 0.0 0.0 0.0 0.0 46 0.47850734635334014 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACAA 15 0.0071383505 52.137905 24 GTACAGC 15 0.0071383505 52.137905 24 TAAGTCA 15 0.0071383505 52.137905 24 CCTAATG 30 7.825256E-7 52.137905 23 ATCACGT 15 0.0071383505 52.137905 24 TTAAGTC 15 0.0071383505 52.137905 23 ACGTGTA 15 0.0071383505 52.137905 27 GATATAA 15 0.0071383505 52.137905 25 ACGCTTC 15 0.0071383505 52.137905 24 CGTACGT 15 0.0071383505 52.137905 23 CGTCGTG 15 0.0071383505 52.137905 25 TACAGCC 15 0.0071383505 52.137905 25 GTACGTC 15 0.0071383505 52.137905 24 TACGTCC 15 0.0071383505 52.137905 25 TCACGTG 15 0.0071383505 52.137905 25 ACGCGAT 40 1.8408173E-9 52.1379 24 ATGTAGA 85 0.0 52.1379 27 CTAAAGG 20 3.3976804E-4 52.1379 23 TAGTGTG 20 3.3976804E-4 52.1379 26 AATACCC 25 1.6257241E-5 52.1379 27 >>END_MODULE