FastQCFastQC Report
Tue 31 May 2016
SRR212595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249510
Sequences flagged as poor quality0
Sequence length58
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT62642.5105206204160155No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT31041.2440383150975913No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC24961.000360706985692No Hit
AGTTTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC22700.9097831750230452No Hit
AGTTTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG18680.748667388080638No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGTGG18490.7410524628271412No Hit
AGTTTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC15050.6031822371848824No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.40038475411807145No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8830.35389363151777486No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG8160.327041000360707No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC8130.32583864374173377No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6740.27012945372930947No Hit
AGTTTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5310.21281712155825416No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC5300.21241633601859644No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGA5230.20961083724099233No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5090.2039998396857841No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.20279748306681095No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.1811550639252936No Hit
AGTTTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCAACCGACTGCTCTTCCA4320.173139353132139No Hit
AGTTTAGGGGGTAATCTTGTGGTAACATGTAGATTTTTATCAGAATAATCTCTGAAAT4300.17233778205282352No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGTTCTTCCGA4280.17153621097350807No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.16832992665624624No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC4100.16432207125966894No Hit
AGTTTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT3790.15189771953027933No Hit
AGTTTAGGGGAGTGAAAAAGAGAGAGGAGAGGAGGAGGAAGCCAACTTTGTGCTAAGG3670.1470882930543866No Hit
AGTTTAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3570.1430804376578093No Hit
AGTTTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC3540.14187808103883612No Hit
AGTTTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3530.1414772954991784No Hit
AGTTTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC3480.13947336780088976No Hit
AGTTTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3470.139072582261232No Hit
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA3390.13586629794397018No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC3360.134663941324997Illumina Single End Adapter 2 (96% over 25bp)
AGTTTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA3340.13386237024568154No Hit
AGTTTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT3170.12704901607150015No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGG3000.12023566189731875No Hit
AGTTTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2990.119834876357661No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG2970.11903330527834556No Hit
AGTTTAGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2810.11262073664382188No Hit
AGTTTAGGGAAGGGTGTTTCTCTCGAACATGCCATCTGGTGCCAAATGAAAATTCTTA2760.11061680894553325No Hit
AGTTTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2760.11061680894553325No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT2700.10821209570758686No Hit
AGTTTAGGGGAACTCCAGTGTTTCAGAGACTCAGTCTCTACAGCGGGGAACGGGTACA2590.10380345477135186No Hit
AGTTTAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2570.10300188369203639No Hit
AGTTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2510.10059717045409003No Hit
AGTTTAGGGCATTGACTGTTTGTGGTCAGGTGTCCAGGGAAAAAAGTTATTTTAGGTT2510.10059717045409003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA203.3361613E-452.331189
ACAGTTA203.3361613E-452.3311810
AGGGTCT458.731149E-1152.3311736
CTCGATG150.007034643652.33117310
GCTACGA150.007034643652.3311739
TGTAACC150.007034643652.33117310
AGGGACC900.052.3311736
GCTCACG150.007034643652.3311739
CGTCTAT203.4169212E-452.0783239
ATAGTTT401.8608262E-952.0783239
ATAGGTT150.007170712652.07831639
GCCGGAT150.007170712652.07831638
TTAAGCG150.007170712652.07831639
CACCGCG150.007170712652.07831638
GATAGTT353.821333E-852.07831638
GATAGGT150.007170712652.07831638
TGATAGT251.6465498E-552.02594437
ATCTCCT251.6465498E-552.02594444
GTAGGGC307.9422716E-752.02594444
GAAACTC150.00719930552.02594452
Produced by FastQC (version 0.11.5)