FastQCFastQC Report
Tue 31 May 2016
SRR212594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234647
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT69402.957634233550823No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC27811.1851845538191412No Hit
AGTTTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA20620.8787668284699997No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC19020.8105792957080209No Hit
AGTTTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA17380.7406870746269929No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGT14040.5983455999863625No Hit
AGTTTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13490.5749061355994323No Hit
AGTTTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC12990.553597531611314No Hit
AGTTTAGGGGGTAATCTTGTGGTAACATGTAGATTTTTATCAGAATAATCTCTGAA12920.5506143270529774No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12030.5126850119541269No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.4606920182231181No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9710.4138130894492578No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC8580.36565564443611037No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT8050.343068524208705No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.2774380239253006No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.22714971851334134No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5300.22587120227405422No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.1994485333287875No Hit
AGTTTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4450.18964657549425307No Hit
AGTTTAGGGGAGTGAAAAAGAGAGAGGAGAGGAGGAGGAAGCCAACTTTGTGCTAA4420.18836805925496597No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGTTCTTCC4300.18325399429781758No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTA4200.17899227350019392No Hit
AGTTTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCAACCGACTGCTCTTC4150.1768614131013821No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.16791179942637238No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT3790.1615192182299369No Hit
AGTTTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3740.15938835783112507No Hit
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC3660.1559789811930261No Hit
AGTTTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3280.13978444216205618No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3250.13850592592276909No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGG3250.13850592592276909No Hit
AGTTTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG3060.13040865640728413No Hit
AGTTTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3060.13040865640728413No Hit
AGTTTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3040.1295563122477594No Hit
AGTTTAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2950.12572076352989808No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2890.12316373105132392No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC2870.12231138689179917No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG2810.11975435441322498Illumina Single End Adapter 2 (95% over 23bp)
AGTTTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2710.1154926336156013No Hit
AGTTTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2600.11080474073821528No Hit
AGTTTAGGGCATTGACTGTTTGTGGTCAGGTGTCCAGGGAAAAAAGTTATTTTAGG2590.11037856865845291No Hit
AGTTTAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2540.10824770825964107No Hit
AGTTTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2530.1078215361798787No Hit
AGTTTAGGGAAGGGTGTTTCTCTCGAACATGCCATCTGGTGCCAAATGAAAATTCT2500.10654301994059161No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG2460.10483833162154214Illumina Single End Adapter 2 (96% over 30bp)
AGTTTAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC2460.10483833162154214No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCACCGACTGCTCTTCC2450.10441215954177978No Hit
AGTTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2420.10313364330249268No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGA2370.10100278290368085No Hit
AGTTTAGGGGTTAAGGGTGTACCAGCTCACTAAGAAACACTGCTATCAAGATTTTT2360.10057661082391847No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2350.1001504387441561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG252.013449E-550.28500750
GCTTGAG150.00823548950.28500749
TATTACT301.004104E-650.28500750
TCCAAAT204.0614468E-450.28500750
CTCTGAA1300.050.28500750
ATTATAT204.0614468E-450.28500750
CTGATGA150.00823548950.28500750
ACCCCGC150.00823548950.28500750
TTATTAC301.004104E-650.28500749
ACACCTC150.00824249450.27419348
CCCAGTA252.0160136E-550.27419348
CAGATAG150.00824249450.27419348
AAATTCG402.5411282E-950.27419348
CGAACAG150.00826353950.2417847
CGGGTAT204.0830532E-450.23098446
TCTTGCC150.00827056350.23098442
TTATGGC150.00827056350.23098444
CATCTTA301.01158E-650.23098445
GTAGGAC150.00827056350.23098445
TGCGAAA252.0262985E-550.23098444
Produced by FastQC (version 0.11.5)