FastQCFastQC Report
Tue 31 May 2016
SRR212593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214605
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT62532.9137252160946856No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC26801.2488059458074137No Hit
AGTTTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC21511.002306563220801No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC19870.9258870948952728No Hit
AGTTTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA19330.900724587031989No Hit
AGTTTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA17290.8056662239929173No Hit
AGTTTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC12970.6043661610866476No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11390.5307425269681508No Hit
AGTTTAGGGGGTAATCTTGTGGTAACATGTAGATTTTTATCAGAATAATCTCTGAA11320.5274807203932806No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.46364250599939427No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGT9400.4380140257682719No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8990.4189091586868899No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC8080.3765056732135784No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT7650.3564688613965192No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5340.24882924442580556No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.24277160364390393No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.21574520630926586No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.21155145499871858No Hit
AGTTTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4500.20968756552736423No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTA4340.20223200764194682No Hit
AGTTTAGGGGAGTGAAAAAGAGAGAGGAGAGGAGGAGGAAGCCAACTTTGTGCTAA3930.18312714056056476No Hit
AGTTTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCAACCGACTGCTCTTC3810.1775354721465017No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGTTCTTCC3670.1710118589967615No Hit
AGTTTAGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3650.17007991426108432No Hit
AGTTTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3620.16868199715756857No Hit
AGTTTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3340.1556347708580881No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT3290.15330490901889518No Hit
AGTTTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3000.13979171035157614No Hit
AGTTTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2990.13932573798373757No Hit
AGTTTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2980.138859765615899No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790.1300062906269658No Hit
AGTTTAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2730.12721045641993428No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2720.1267444840520957No Hit
AGTTTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA2640.12301670510938702No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC2630.12255073274154843No Hit
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2590.12068684327019408No Hit
AGTTTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2560.11928892616667833No Hit
AGTTTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2530.11789100906316255No Hit
AGTTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2450.11416323012045386No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG2440.11369725775261527Illumina Single End Adapter 2 (95% over 23bp)
AGTTTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2430.1132312853847767No Hit
AGTTTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2420.11276531301693808No Hit
AGTTTAGGGGAGTGCAGTGCTAGTTTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGA2410.11229934064909951No Hit
AGTTTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2400.11183336828126092No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGGGGTTAAGAGCACCGACTGCTCTTCC2310.10763961697071364No Hit
AGTTTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2290.10670767223503645No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGG2290.10670767223503645No Hit
AGTTTAGGGCATTGACTGTTTGTGGTCAGGTGTCCAGGGAAAAAAGTTATTTTAGG2290.10670767223503645No Hit
AGTTTAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC2260.1053097551315207No Hit
AGTTTAGGGACACCCAGTGGCTCACAAATACCCTAACTCCAGCTCCAGGGCTCCAG2160.10065003145313482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAAAC150.00829035750.19915849
CTCGTCA150.00829035750.19915850
CGCGCGA150.00829035750.19915849
TATTACT252.0333284E-550.19915850
CATGTGT150.00829035750.19915850
CATCGAA252.0333284E-550.19915850
TTATTAC252.0333284E-550.19915849
CGGACTC252.0333284E-550.19915850
ACGGGTA204.0950617E-450.19915450
GGTCGTC150.00830574550.17561748
GAACGGG204.104554E-450.17561748
CGATGCA150.00830574550.17561748
ATTTGAC150.00830574550.17561748
CCCATCG150.00830574550.17561748
TAATAAG301.0205458E-650.16385744
TCGGGAA301.0205458E-650.16385745
ATCTTAT252.0418116E-550.16385746
CTTTAGC150.00831344750.16385745
TTACACC150.00831344750.16385746
AATAAGC301.0205458E-650.16385745
Produced by FastQC (version 0.11.5)