##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 594376 Sequences flagged as poor quality 0 Sequence length 58 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.477182793383314 39.0 37.0 39.0 33.0 40.0 2 36.70066590844852 39.0 36.0 39.0 31.0 40.0 3 36.153736018950966 38.0 35.0 39.0 30.0 40.0 4 36.48152516252338 39.0 35.0 39.0 30.0 40.0 5 36.61221516346555 39.0 35.0 39.0 31.0 40.0 6 36.222993525983554 38.0 35.0 40.0 30.0 40.0 7 35.82426948598194 38.0 35.0 39.0 29.0 40.0 8 35.51238946390837 38.0 35.0 40.0 26.0 40.0 9 35.196485053232294 38.0 34.0 40.0 25.0 40.0 10 35.229904975974804 38.0 33.0 39.0 28.0 40.0 11 35.67538056718306 38.0 35.0 39.0 29.0 40.0 12 35.61856299716005 38.0 35.0 39.0 29.0 40.0 13 35.412496466882914 38.0 34.0 39.0 28.0 40.0 14 35.818831850545784 38.0 35.0 39.0 29.0 40.0 15 35.587128349731486 38.0 35.0 39.0 29.0 40.0 16 35.496256578327525 38.0 35.0 39.0 29.0 40.0 17 35.3704002180438 38.0 35.0 39.0 28.0 40.0 18 35.14247547007282 38.0 34.0 39.0 28.0 40.0 19 34.94021461162631 38.0 34.0 39.0 27.0 40.0 20 35.10239646284507 38.0 34.0 39.0 28.0 40.0 21 35.322262338990804 38.0 35.0 39.0 28.0 40.0 22 35.15745083919943 38.0 35.0 39.0 28.0 40.0 23 34.96413381428591 38.0 35.0 39.0 27.0 40.0 24 34.89530532861354 38.0 34.0 39.0 27.0 40.0 25 34.80553555325249 38.0 35.0 39.0 27.0 40.0 26 34.47625240588449 38.0 35.0 39.0 26.0 40.0 27 34.4075938463195 38.0 35.0 39.0 26.0 40.0 28 33.69634204611223 38.0 33.0 39.0 23.0 40.0 29 33.75671628733327 38.0 33.0 39.0 23.0 40.0 30 32.95407116034295 38.0 33.0 39.0 16.0 40.0 31 31.807179293914963 37.0 32.0 39.0 2.0 40.0 32 30.963746853843357 36.0 30.0 39.0 2.0 40.0 33 31.0496655315827 36.0 30.0 39.0 2.0 40.0 34 30.686269297549025 36.0 29.0 39.0 2.0 40.0 35 30.343720136748455 36.0 29.0 39.0 2.0 40.0 36 30.139320901247697 37.0 29.0 39.0 2.0 40.0 37 29.98861495080555 37.0 28.0 39.0 2.0 40.0 38 29.913514341090487 36.0 28.0 39.0 2.0 40.0 39 29.77048030203104 36.0 28.0 39.0 2.0 40.0 40 29.619397485766587 36.0 28.0 39.0 2.0 40.0 41 29.830156332018788 37.0 29.0 39.0 2.0 40.0 42 29.764813855202767 37.0 29.0 39.0 2.0 40.0 43 29.653135389046664 36.0 29.0 39.0 2.0 40.0 44 29.515740877828176 36.0 28.0 39.0 2.0 40.0 45 29.383588839387862 36.0 28.0 39.0 2.0 40.0 46 29.179574881892943 36.0 27.0 39.0 2.0 40.0 47 29.0588381765078 36.0 27.0 39.0 2.0 40.0 48 28.887000821029112 36.0 26.0 39.0 2.0 40.0 49 28.690049732829053 36.0 25.0 39.0 2.0 40.0 50 28.39040270805012 36.0 23.0 39.0 2.0 40.0 51 27.843812670767324 35.0 17.0 39.0 2.0 40.0 52 27.719322449089464 35.0 14.0 39.0 2.0 40.0 53 27.501322395251492 35.0 2.0 39.0 2.0 40.0 54 27.121579606175217 35.0 2.0 39.0 2.0 40.0 55 26.739153330551705 35.0 2.0 39.0 2.0 40.0 56 26.09979036838634 35.0 2.0 39.0 2.0 40.0 57 25.80948086732977 35.0 2.0 39.0 2.0 40.0 58 25.49191925649757 35.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -1.4748154401528666 1 2 -2.4288822767711196 1 3 -1.8094579406500344 1 4 -1.96125458591915 1 5 -2.6584466129017628 1 6 -3.3082255574055637 1 7 -3.6854405886149593 1 8 -3.617515714810164 1 9 -3.4983621117885555 1 10 -2.2497307403352025 1 11 -1.5765504107631045 1 12 -1.7360983015488571 1 13 -2.455547775262694 1 14 -1.3288848101847037 1 15 -1.6082142952697893 1 16 -2.0155107943438395 1 17 -2.12005203942622 1 18 -2.299476954606142 1 19 -2.296947074106747 1 20 -2.0808257026728043 1 21 -2.1271047614058602 1 22 -2.052032009916921 1 23 -1.9423810714795806 1 24 -1.9383605305717424 1 25 -2.246138257040826 1 26 -1.7943875578548543 1 27 -1.6390709510273638 1 28 -1.94186003696883 1 29 -1.9893527550288148 1 30 -1.7557766874595444 1 31 -1.8624415498484872 1 32 -1.7019988531380363 1 33 -1.623331550767638 1 34 -2.0093605467975273 1 35 -2.740824786582042 1 36 -2.8150633540213867 1 37 -2.5934887348344056 1 38 -2.7030573783576877 1 39 -2.3637001386492855 1 40 -2.042808479504295 1 41 -2.2794686101082107 1 42 -2.2206379022939444 1 43 -2.314956508410006 1 44 -2.199137940888061 1 45 -1.88056685964294 1 46 -1.8739767191757224 1 47 -1.9363585825612724 1 48 -1.87653210209956 1 49 -2.019915740497055 1 50 -2.2650026171130406 1 51 -1.80543718105951 1 52 -2.217789837872701 1 53 -1.9889154784191945 1 54 -2.327712741416871 1 55 -2.0807222697002494 1 56 -2.0447386235075236 1 57 -2.0460908544112826 1 58 -2.347779738738293 2 1 -0.0426457325035301 2 2 -0.3674639476091528 2 3 -0.46251965912394155 2 4 -0.07240012223424941 2 5 -0.5267121151359007 2 6 -1.5905165332148599 2 7 -1.6590859433908918 2 8 -1.9056498311017265 2 9 -1.89875036354222 2 10 -0.7184067631268434 2 11 -0.6093726911854418 2 12 -0.4413706829069639 2 13 -0.6662542288588611 2 14 -0.31510235703390777 2 15 -0.8693983178871463 2 16 -0.20862489291717878 2 17 -0.5109663674650662 2 18 -0.6322358173480112 2 19 -0.8681951437403228 2 20 -0.5326839375312247 2 21 -0.7320658636148281 2 22 -0.6226168564800219 2 23 -0.44721485422319773 2 24 -0.40822745026365226 2 25 -0.8643880258809631 2 26 -0.8320557236315764 2 27 -0.604559146140808 2 28 -0.5510618939934275 2 29 -0.7819478936521165 2 30 -1.6482861395985609 2 31 -1.2008111829457384 2 32 -0.7990327452892529 2 33 -0.8278850370457 2 34 -1.2175254521309071 2 35 -1.6677492227214046 2 36 -1.1034879329715572 2 37 -1.6004549975966071 2 38 -1.3317638029075916 2 39 -1.489267145964103 2 40 -1.7120053986636954 2 41 -1.771120229195482 2 42 -1.7919924749023508 2 43 -1.7411959367794942 2 44 -1.538867907436444 2 45 -1.568367912085975 2 46 -1.466898144185528 2 47 -1.2814330620279826 2 48 -1.2419970602003225 2 49 -1.3353485056531547 2 50 -1.5006646541276645 2 51 -1.2891494874782516 2 52 -1.1642720930247812 2 53 -1.5509558817750069 2 54 -1.3400912917177408 2 55 -1.5092611941170233 2 56 -1.1971008110786272 2 57 -0.9916387880115174 2 58 -0.97789974822674 3 1 -0.3098932214451011 3 2 -0.38266878317860886 3 3 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-0.6822423164659526 118 15 -0.5691954781305526 118 16 -0.4155214446413211 118 17 -0.4156316818666852 118 18 -0.40505528996465046 118 19 -0.8418259753833297 118 20 -0.4515480348943086 118 21 -0.5109705289292279 118 22 -0.5313389660134931 118 23 -0.5040099860690361 118 24 -0.4447386064502652 118 25 -0.5215888372399888 118 26 -0.9983843240372536 118 27 -0.9372163469521695 118 28 -1.4293580400380534 118 29 -1.5060858246866005 118 30 -1.5570082491320285 118 31 -1.41784972249949 118 32 -1.5961929886969592 118 33 -1.982043252055842 118 34 -1.93557819046762 118 35 -1.3269784316463564 118 36 -1.6917232270892057 118 37 -1.8763170665621267 118 38 -2.031560963150998 118 39 -1.7793279978910803 118 40 -1.7708289280754599 118 41 -2.0348119127654627 118 42 -2.116536085450015 118 43 -2.1448470523981555 118 44 -1.8147299764019635 118 45 -2.0470697376437847 118 46 -1.9902247161936408 118 47 -1.9163215001618568 118 48 -1.7795224151698967 118 49 -1.7085736577829707 118 50 -1.6101981226875033 118 51 -1.4940176416364643 118 52 -1.3650834520511488 118 53 -0.9992317438439748 118 54 -0.841105490500702 118 55 -0.8845147438127618 118 56 -0.3897985798413046 118 57 -0.4743974087011722 118 58 -0.23144944396710443 119 1 -0.41239943869888407 119 2 -0.7059136665195922 119 3 -1.0776363021462956 119 4 -0.7665836210777783 119 5 -0.8541458873456378 119 6 -1.6898958191701396 119 7 -2.001547743258378 119 8 -1.7420894446086592 119 9 -1.9198351294228715 119 10 -0.852736274075717 119 11 -0.6340305261250947 119 12 -0.7822430971065586 119 13 -1.043649994020015 119 14 -0.6918924483727693 119 15 -0.8319888132795583 119 16 -0.9797208373415245 119 17 -0.7758409442047522 119 18 -1.0332691212783942 119 19 -0.7947541440939716 119 20 -0.5896619910012504 119 21 -1.1097095891998734 119 22 -1.1116070071667892 119 23 -1.03848258237727 119 24 -1.0613511418659272 119 25 -1.2243046136203333 119 26 -0.7057137902719575 119 27 -0.9888205695962426 119 28 -1.4061107340670844 119 29 -0.8795101581405262 119 30 -1.0919239222632804 119 31 -1.9146892433261335 119 32 -2.029221317159159 119 33 -1.9640559590113327 119 34 -2.541235753057304 119 35 -2.240415854158723 119 36 -1.4902370905158548 119 37 -1.456694860689936 119 38 -1.4406997274951046 119 39 -1.4081231134772239 119 40 -1.5260757299957284 119 41 -1.6914476995520147 119 42 -1.5288820756047699 119 43 -1.3690886390196724 119 44 -1.3457270618507486 119 45 -1.4433917935202416 119 46 -1.3348124718266092 119 47 -1.093747345767877 119 48 -0.9205102732389747 119 49 -0.9665297322036182 119 50 -1.128093954919919 119 51 -0.7862532284287091 119 52 -1.0222436141603595 119 53 -0.6439247810339737 119 54 -0.7637255785469783 119 55 -0.547211333747196 119 56 -0.7016514945957617 119 57 -0.6191941014782074 119 58 -0.7623367764413835 120 1 -2.345207410532275 120 2 -3.7520410089236975 120 3 -3.044532500558155 120 4 -3.3416686281847205 120 5 -3.7570343710800245 120 6 -3.8906482011270356 120 7 -4.072823126104268 120 8 -4.354256133876923 120 9 -4.53826497156329 120 10 -3.1733524437584393 120 11 -2.459581377200678 120 12 -3.1878362382761196 120 13 -3.1557589236587447 120 14 -2.996089815538177 120 15 -2.837121886443427 120 16 -3.454645624258717 120 17 -3.2602533780950864 120 18 -3.0462424363295426 120 19 -3.335539976410235 120 20 -3.1924113493671022 120 21 -3.3913154073524687 120 22 -3.1261373209271426 120 23 -3.1147152482101923 120 24 -3.268954318143294 120 25 -3.4641175728721727 120 26 -2.937400220777075 120 27 -3.601147981311904 120 28 -2.973400702475079 120 29 -3.3858511609481496 120 30 -3.150678021941335 120 31 -2.978938041674713 120 32 -3.138537694274291 120 33 -3.061288017112499 120 34 -3.2008356546148065 120 35 -3.4474062761053723 120 36 -3.1415597860322 120 37 -3.1957820576402263 120 38 -3.3652857416286324 120 39 -3.437530298775137 120 40 -3.3415864506852273 120 41 -3.235247077757048 120 42 -3.349827224047015 120 43 -3.3494101843660076 120 44 -3.1376417182311798 120 45 -3.265999414777795 120 46 -3.165983585984751 120 47 -3.218603574516102 120 48 -3.2694236388172513 120 49 -3.2132989598820316 120 50 -3.488885713876428 120 51 -3.078979030910869 120 52 -3.1467231496724857 120 53 -3.0034207152583114 120 54 -3.0247237653968675 120 55 -3.1667009395251675 120 56 -2.9295760153163997 120 57 -2.9489865183922355 120 58 -2.5615241235667128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2597.0 3 320.0 4 644.0 5 458.0 6 985.0 7 2034.0 8 1557.0 9 2451.0 10 3004.0 11 2984.0 12 4373.0 13 5320.0 14 6146.0 15 6804.0 16 7404.0 17 7565.0 18 9196.0 19 9052.0 20 10006.0 21 8660.0 22 10784.0 23 8473.0 24 6566.0 25 6656.0 26 6916.0 27 8004.0 28 9145.0 29 11421.0 30 13993.0 31 17378.0 32 21214.0 33 25705.0 34 30760.0 35 37443.0 36 46471.0 37 67302.0 38 85557.0 39 88702.0 40 326.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 39.328472212875354 45.9244653216146 5.048151338546644 9.698911126963404 11 28.068428065736168 48.91617427352383 13.68594963457475 9.329448026165256 12 59.41811244060998 14.002920710122885 17.612756908085117 8.966209941182013 13 23.43196898932662 16.214483761120906 24.65038965234128 35.703157597211195 14 28.028890803127986 36.59081120368251 18.261504502200626 17.118793490988867 15 40.19459062950052 21.16522201434782 18.076772951801555 20.56341440435011 16 26.301417801339568 23.89870972843561 37.366728861266786 12.43314360895804 17 49.01223808578437 16.674518064383705 19.00671967481754 15.306524175014385 18 42.47049421661409 16.442145110410095 25.624058885383803 15.463301787592009 19 28.697540917411434 16.29546005168515 37.99447480348875 17.012524227414666 20 17.212347988774557 36.184055561911556 25.056026273462052 21.547570175851835 21 19.13764181530318 21.73124454232965 46.63336401125942 12.497749631107753 22 19.93724447734576 24.093241583525412 24.128404865656073 31.84110907347275 23 24.385816146953225 34.330698593122754 27.79280779856183 13.490677461362191 24 27.552256484111066 35.095461458739926 20.479292569013555 16.872989488135456 25 19.364206098843322 14.143259726603574 27.082060988433227 39.41047318611987 26 39.894995456534176 12.91236159256891 32.91690505839195 14.275737892504964 27 26.398411670186007 13.413310674956044 27.255840561299603 32.93243709355835 28 36.54929968547691 31.76531764476224 20.230280041667157 11.455102628093695 29 54.84115231877229 15.806353907249107 19.04338022480985 10.30911354916874 30 38.888823431390534 33.379901667875764 16.90969165590826 10.821583244825444 31 51.12802835723927 20.53324670865495 17.90673484432805 10.431990089777726 32 32.61897830853339 24.86012697525951 31.900977470557258 10.619917245649843 33 38.28452586874146 28.0627216863983 19.555516668124945 14.097235776735296 34 23.718269093234387 33.306408864881234 21.92644571774743 21.048876324136945 35 19.504918005199876 50.70080522343478 20.318723758719045 9.4755530126463 36 26.594810029857978 41.17876750832287 19.569432429066254 12.656990032752894 37 18.281370715671937 41.73970271648659 29.718083933527794 10.260842634313674 38 25.436323752476863 39.99161611927333 23.627560408554253 10.944499719695553 39 18.21640183140318 39.34537436035551 23.804538109345543 18.63368569889577 40 17.962823927088838 39.15069406334275 30.551917554095866 12.334564455472545 41 15.07048773303034 46.05495939064933 25.980389681437526 12.894163194882802 42 16.10109847183777 40.21208386371137 31.12409835734346 12.562719307107404 43 23.809050759617925 37.84754435384069 25.069664616436306 13.273740270105083 44 16.331570318049753 39.154931000003366 26.02287992081581 18.490618761131067 45 17.233566579528986 38.59604530710562 32.36488749812735 11.80550061523804 46 16.753448110420337 38.828088569259336 32.211992505315465 12.206470815004856 47 23.778664007976072 38.28194470642128 26.154307348225593 11.785083937377058 48 24.8905840848047 38.05366810646055 24.976298129951925 12.079449678782826 49 26.674953847812322 38.60546280201048 23.65080648421393 11.068776865963267 50 17.11225352634611 43.0785245774617 27.091804593093176 12.717417303099007 51 16.506765689073013 44.95549885905306 26.105202886469463 12.432532565404468 52 21.048306844185213 40.19003487171888 27.02364575317693 11.738012530918972 53 16.01072591973751 40.96230095232962 26.492415386843902 16.534557741088964 54 14.909529472404046 45.00197077857545 25.922307529047927 14.16619221997258 55 19.935770417997762 41.58090366236672 24.03234394166094 14.450981977974578 56 17.261182560867102 42.69122920542479 26.285927094286716 13.761661139421392 57 22.24717927287923 41.922003693568605 23.43629942170039 12.394517611851771 58 21.56266008789668 42.701171506376554 23.11079430558926 12.625374100137506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 879.5 9 2413.0 10 3067.0 11 2552.5 12 2038.0 13 1635.5 14 1233.0 15 1433.0 16 1827.0 17 2021.0 18 2126.5 19 2232.0 20 2569.0 21 2906.0 22 3830.5 23 6129.0 24 7503.0 25 8829.5 26 10156.0 27 13887.5 28 22938.5 29 28258.0 30 32533.5 31 36809.0 32 43659.5 33 50510.0 34 39600.5 35 25116.0 36 21541.0 37 19793.0 38 18045.0 39 16600.0 40 15155.0 41 14956.5 42 15197.0 43 15636.0 44 16283.0 45 16930.0 46 16566.5 47 17253.5 48 18304.0 49 20194.5 50 22085.0 51 25065.5 52 28046.0 53 35051.0 54 41697.5 55 41339.0 56 37522.0 57 33705.0 58 31210.0 59 25224.5 60 21734.0 61 19640.5 62 17547.0 63 13486.0 64 9425.0 65 7058.0 66 3719.0 67 2747.0 68 2160.0 69 1573.0 70 1424.0 71 1275.0 72 988.0 73 588.0 74 475.0 75 350.5 76 226.0 77 174.0 78 107.5 79 93.0 80 59.5 81 26.0 82 18.5 83 11.0 84 12.0 85 8.5 86 4.0 87 3.5 88 3.0 89 2.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 8.412183533655464E-4 17 3.3648734134621855E-4 18 1.6824367067310927E-4 19 0.0013459493653848742 20 0.0020189240480773113 21 0.004879066449520169 22 0.0010094620240386556 23 0.0016824367067310927 24 0.0 25 1.6824367067310927E-4 26 0.019516265798080676 27 0.00689799049759748 28 0.024058844906254626 29 0.01615139238461849 30 0.04492106006972018 31 0.040714968302892444 32 0.014637199348560507 33 0.01144056960577143 34 0.020357484151446222 35 0.022039920858177315 36 0.03869604425481513 37 0.04424808538702774 38 0.06376435118510843 39 0.04980012651924035 40 0.056866360687510935 41 0.050641344872605895 42 0.07739208850963027 43 0.07722384483895717 44 0.05350148727404875 45 0.04996837018991345 46 0.05972650308895379 47 0.10094620240386556 48 0.09101982583415212 49 0.11575164542309917 50 0.11810705681252272 51 0.0960671359543454 52 0.081598180276458 53 0.11339623403367564 54 0.11810705681252272 55 0.14805443019233616 56 0.16016797448080003 57 0.15343822765387566 58 0.16016797448080003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 594376.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.278400145849986 #Duplication Level Percentage of deduplicated Percentage of total 1 76.40973036645052 30.776616947363983 2 11.379996435878109 9.167361002052903 3 4.6727895935224515 5.6463746713578296 4 2.203817530575877 3.550649773798966 5 1.242581607591309 2.502459960221814 6 0.7648406480087874 1.848393460098548 7 0.5590622778062841 1.5762693892352302 8 0.4091041361188109 1.3182448076732596 9 0.2944021547195852 1.0672243012436302 >10 1.7734312783327766 13.149923633906704 >50 0.1644854642038663 4.60074803032329 >100 0.10035695409534137 8.114793672317617 >500 0.01165972910651269 3.410390437139251 >1k 0.012492566899835024 9.15084254509631 >5k 8.32837793322335E-4 1.7188649584322553 >10k+ 4.164188966611675E-4 2.4008424097384173 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 14314 2.4082399020148864 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGTGG 5157 0.8676326096612246 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 5091 0.8565285273967993 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3611 0.6075278948005975 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3553 0.5977697619015573 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 3545 0.5964238125361724 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 2527 0.4251517557909471 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 2495 0.41976795832940766 No Hit ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 2460 0.4138794298558488 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2227 0.37467865458901434 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 2170 0.3650887653606471 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2096 0.35263873373083704 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 2033 0.34203938247843113 Illumina Single End Adapter 2 (96% over 25bp) ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA 1893 0.31848526858419585 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 1873 0.31512039517073365 No Hit ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1805 0.30367982556496226 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1614 0.27154528446639836 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1590 0.26750743637024377 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1479 0.24883238892552864 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 1442 0.24260737311062358 No Hit ATGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1437 0.24176615475725804 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1423 0.2394107433678345 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA 1354 0.22780193009138996 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 1335 0.22460530034860088 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1333 0.22426881300725465 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA 1325 0.22292286364186978 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAA 1253 0.2108093193534059 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 1207 0.2030701105024429 No Hit ATGCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1135 0.19095656621397902 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1128 0.18977886051926726 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 1124 0.1891058858365748 No Hit ATGCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1052 0.17699234154811097 No Hit ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1039 0.17480517382936053 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 999 0.16807542700243616 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 948 0.1594949997981076 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 945 0.15899026878608827 Illumina Single End Adapter 2 (96% over 32bp) ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 903 0.1519240346178177 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGGGG 898 0.15108281626445214 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA 862 0.14502604412022022 No Hit ATGCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 848 0.14267063273079666 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 841 0.1414929270360849 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA 830 0.1396422466586807 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 790 0.13291249983175632 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 781 0.13139830679569836 No Hit ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 757 0.12736045869954374 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 734 0.1234908542740622 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 700 0.11777056947117649 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC 693 0.11659286377646472 Illumina Single End Adapter 2 (100% over 31bp) ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 681 0.11457393972838742 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA 677 0.11390096504569498 No Hit ATGCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 639 0.10750770556011682 No Hit ATGCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 637 0.10717121821877061 No Hit ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 633 0.10649824353607816 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 628 0.10565702518271261 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 599 0.10077795873319245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.033648734134621855 0.0 0.0 0.0 0.0 11 0.06864341763462858 0.0 0.0 0.0 0.0 12 0.07184004737741766 0.0 0.0 0.0 0.0 13 0.07554140813222607 0.0 0.0 0.0 0.0 14 0.10027322772117313 0.0 0.0 0.0 0.0 15 0.10717121821877061 0.0 0.0 0.0 0.0 16 0.1144056960577143 0.0 0.0 0.0 0.0 17 0.11844354415386893 0.0 0.0 0.0 0.0 18 0.12315436693271599 0.0 0.0 0.0 0.0 19 0.1283699207235824 0.0 0.0 0.0 0.0 20 0.16336460422358912 0.0 0.0 0.0 0.0 21 0.17329098079330255 0.0 0.0 0.0 0.0 22 0.1870869617884975 0.0 0.0 0.0 0.0 23 0.20912688264667484 0.0 0.0 0.0 0.0 24 0.23671884463706475 0.0 0.0 0.0 0.0 25 0.24513102817072022 0.0 0.0 0.0 0.0 26 0.2547209173990874 0.0 0.0 0.0 0.0 27 0.26414256295678157 0.0 0.0 0.0 0.0 28 0.417244303269311 0.0 0.0 0.0 0.0 29 0.4300308222404673 0.0 0.0 0.0 0.0 30 0.48908435064672867 0.0 0.0 0.0 0.0 31 0.5010296512645195 0.0 0.0 0.0 0.0 32 0.5146573885890413 0.0 0.0 0.0 0.0 33 0.529631075278948 0.0 0.0 0.0 0.0 34 0.5476331480409707 0.0 0.0 0.0 0.0 35 0.7259714389544666 0.0 0.0 0.0 0.0 36 0.7419545876684119 0.0 0.0 0.0 0.0 37 0.7560870560049531 0.0 0.0 0.0 0.0 38 0.7742573724376489 0.0 0.0 0.0 0.0 39 0.7895675464689018 0.0 0.0 0.0 0.0 40 0.809925030620348 0.0 0.0 0.0 0.0 41 0.8312919767958329 0.0 0.0 0.0 0.0 42 0.8548460906900682 0.0 0.0 0.0 0.0 43 0.8846252203992085 0.0 0.0 0.0 0.0 44 0.9202928785819078 0.0 0.0 0.0 0.0 45 0.957642973471338 0.0 0.0 0.0 0.0 46 1.0030687645530776 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGAACT 25 1.6383101E-5 52.084995 52 ACGCCAG 15 0.0071743536 52.0806 51 GGTCGCC 25 1.6432221E-5 52.058655 48 CACGACC 15 0.007186321 52.05865 48 CTATTAT 15 0.007186321 52.05865 48 CACACGA 15 0.007191112 52.04988 47 GTTGACG 15 0.0071935086 52.045494 46 ACACACG 15 0.0071935086 52.045494 46 CTCCCAA 25 1.6465036E-5 52.041115 44 CGTAGGG 15 0.0071959053 52.04111 43 CCGAATC 15 0.007198303 52.036724 42 CTACATC 50 3.6379788E-12 52.023582 37 CGTTATG 15 0.0072054993 52.02358 37 CGTGGTT 15 0.0072151027 52.00606 34 CGAACTG 15 0.0072151027 52.00606 35 ACGTGGT 15 0.0072151027 52.00606 33 CGCAACC 15 0.0072151027 52.00606 35 GTCGACG 15 0.007217505 52.001682 31 TACCTTG 20 3.4471892E-4 51.997307 30 TGCACGG 40 1.893568E-9 51.997307 32 >>END_MODULE