FastQCFastQC Report
Tue 31 May 2016
SRR212591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598128
Sequences flagged as poor quality0
Sequence length58
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT141022.357689324024289No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA52250.8735588369044753No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGTGG40770.6816266752267073No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA36690.6134138512157933No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA36490.6100700853329053No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA35460.5928496910360325No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA24750.41379102800738304No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA24620.4116175801835059No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA24360.40727068453575155No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22170.3706564481181286No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22100.3694861300591178No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA22090.3693189417649734No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC21940.3668111173528074Illumina Single End Adapter 2 (96% over 25bp)
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA18690.3124749217558784No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA18490.3091311558729904No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG18190.3041155070486585No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA17070.285390418104486No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15410.2576371612765161No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15380.25713559639408284No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA14250.23824331915576596No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA13900.23239172886071208No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA13820.2310542225075569No Hit
ATGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13670.22854639809539093No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA13370.22353074927105904No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAA12760.2133322633282508No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA12630.21115881550437363No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA12310.20580879009175296No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA12260.20497284862103096No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11920.19928844662012143No Hit
ATGCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11820.19761656367867747No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11570.19343685632506755No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA10120.16919455367412994No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT9860.1648476580263756Illumina Single End Adapter 2 (96% over 32bp)
ATGCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9790.16367733996736483No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9610.1606679506727657No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA9500.15882887943717733No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA9400.15715699649573336No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA8440.14110692025787122No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA8180.1367600246101169No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA8170.1365928363159725No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA8070.13492095337452853No Hit
ATGCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8020.13408501190380653No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGGGG7820.1307412460209186No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA7790.13023968113848539No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC7700.12873498649118584Illumina Single End Adapter 2 (100% over 31bp)
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA7700.12873498649118584No Hit
ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7260.12137870154883236No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAA7040.11770055907765561No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA6680.11168178048845732No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA6490.10850520289971378No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA6460.10800363801728059No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA6450.10783644972313618No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA6060.1013161062515047No Hit
ATGCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6040.1009817296632159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTT150.007143669852.13711527
CATCGGG150.007143669852.13711527
CGGACGG203.4018094E-452.13711527
ACGCGAA401.8553692E-952.11962524
TACTTAC203.4074503E-452.11962524
CTCACAT150.007153156752.11961724
TAGGTTT150.007153156752.11961724
CGCGAAC307.863E-752.11961725
GCATACC150.007153156752.11961726
CAACTCG203.4159256E-452.09340322
TAACCCC203.4272522E-452.05848351
TCCCGAA203.4272522E-452.05848352
TCAATCC150.007207888652.01925347
TCAAGCG353.867899E-852.01925347
TTTCGTA203.4414526E-452.01490445
CGTAGGG203.4414526E-452.01490443
AGCGTTC150.007210275652.014942
GGTCGCC307.9729034E-752.014948
CGCATGT150.007210275652.014946
AAGCGTT150.007210275652.014941
Produced by FastQC (version 0.11.5)