##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212590_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600739 Sequences flagged as poor quality 0 Sequence length 58 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.26049082879587 38.0 37.0 39.0 33.0 40.0 2 36.47289421862073 38.0 35.0 39.0 30.0 40.0 3 35.94236931512687 38.0 35.0 39.0 29.0 40.0 4 36.16641836138489 38.0 35.0 39.0 30.0 40.0 5 36.40373772969626 38.0 35.0 39.0 30.0 40.0 6 36.04849859922529 38.0 35.0 39.0 29.0 40.0 7 35.6166804552393 38.0 35.0 39.0 29.0 40.0 8 35.44167600239039 38.0 34.0 39.0 27.0 40.0 9 35.08865081175019 38.0 34.0 39.0 25.0 40.0 10 34.30499101939444 38.0 33.0 39.0 25.0 40.0 11 34.99477143984326 38.0 33.0 39.0 27.0 40.0 12 35.12914260602358 38.0 33.0 39.0 28.0 40.0 13 34.94761618606417 38.0 33.0 39.0 27.0 40.0 14 35.157827276071636 38.0 34.0 39.0 28.0 40.0 15 35.17923091392435 38.0 33.0 39.0 28.0 40.0 16 35.11418436292633 38.0 35.0 39.0 27.0 40.0 17 34.81894133725295 38.0 34.0 39.0 26.0 40.0 18 34.77282480411626 38.0 33.0 39.0 27.0 40.0 19 34.607709837383624 38.0 33.0 39.0 26.0 40.0 20 34.72933004183181 38.0 33.0 39.0 27.0 40.0 21 35.02569002511906 38.0 35.0 39.0 28.0 40.0 22 34.90367530658073 38.0 35.0 39.0 27.0 40.0 23 34.69258862833943 38.0 34.0 39.0 27.0 40.0 24 34.63662921834607 38.0 34.0 39.0 27.0 40.0 25 34.519340345807414 38.0 34.0 39.0 26.0 40.0 26 34.1125896604016 38.0 34.0 39.0 25.0 40.0 27 34.02984657230511 38.0 34.0 39.0 24.0 40.0 28 33.372406319549754 38.0 33.0 39.0 21.0 40.0 29 33.205367056242395 38.0 33.0 39.0 18.0 40.0 30 32.45550397094246 38.0 33.0 39.0 9.0 40.0 31 31.426113503534815 37.0 31.0 39.0 2.0 40.0 32 30.619856543357432 36.0 29.0 39.0 2.0 40.0 33 30.646896572388343 36.0 29.0 39.0 2.0 40.0 34 30.336766882123516 36.0 29.0 39.0 2.0 40.0 35 29.84507581495458 36.0 28.0 39.0 2.0 40.0 36 29.696805101716386 36.0 28.0 39.0 2.0 40.0 37 29.56312808058075 36.0 27.0 39.0 2.0 40.0 38 29.486262753042503 36.0 27.0 39.0 2.0 40.0 39 28.952540121417123 36.0 25.0 39.0 2.0 40.0 40 29.178796449040266 36.0 26.0 39.0 2.0 40.0 41 29.24255125770093 36.0 27.0 39.0 2.0 40.0 42 29.184073283073015 36.0 27.0 39.0 2.0 40.0 43 29.056090914690074 36.0 26.0 39.0 2.0 40.0 44 28.67634696598689 36.0 24.0 39.0 2.0 40.0 45 28.70704082804679 36.0 25.0 39.0 2.0 40.0 46 28.392608104351474 36.0 22.0 39.0 2.0 40.0 47 28.418948994488456 36.0 23.0 39.0 2.0 40.0 48 28.28610428155988 35.0 22.0 39.0 2.0 40.0 49 28.155629982405003 35.0 22.0 39.0 2.0 40.0 50 27.618215231573114 35.0 16.0 39.0 2.0 40.0 51 27.32846211083349 35.0 2.0 39.0 2.0 40.0 52 27.211695927848865 35.0 2.0 39.0 2.0 40.0 53 27.00399507939388 35.0 2.0 39.0 2.0 40.0 54 26.637399935745805 35.0 2.0 39.0 2.0 40.0 55 26.30525069955505 35.0 2.0 39.0 2.0 40.0 56 25.658132733183628 35.0 2.0 39.0 2.0 40.0 57 25.456214762151284 35.0 2.0 39.0 2.0 40.0 58 25.15283842067853 34.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.6253944886793619 1 2 -3.5306039733682653 1 3 -3.4221270624589764 1 4 -4.440601970019916 1 5 -3.777762977841249 1 6 -3.485133046340927 1 7 -2.6577159193114994 1 8 -3.0905047314988536 1 9 -5.387528159770962 1 10 -5.472751524807393 1 11 -5.93867948805266 1 12 -5.05194726114852 1 13 -4.761723656654556 1 14 -5.321521187496149 1 15 -4.302853910374623 1 16 -4.608194671753427 1 17 -2.971396111467925 1 18 -3.3661131665428066 1 19 -4.692287026560379 1 20 -4.074003729103449 1 21 -4.419375547706423 1 22 -3.564202956004788 1 23 -3.954011950968212 1 24 -3.5541870162464377 1 25 -3.5545807394986753 1 26 -4.320370222026547 1 27 -3.3942968221042094 1 28 -4.376031779739893 1 29 -3.9482306746777205 1 30 -4.244687937113564 1 31 -4.286110811385438 1 32 -4.221457880153515 1 33 -3.3365546999476443 1 34 -4.410550622923999 1 35 -5.406933639879359 1 36 -4.394898605114726 1 37 -4.898742220614086 1 38 -4.873611073185064 1 39 -4.638484702329059 1 40 -5.532497156760826 1 41 -4.770548582572708 1 42 -4.946751633245302 1 43 -4.542112902589118 1 44 -4.774929243272773 1 45 -5.466205791607557 1 46 -4.330107887023278 1 47 -4.493481934516296 1 48 -4.3467999777457855 1 49 -4.2728087348430215 1 50 -4.244638053838699 1 51 -4.106410783978635 1 52 -3.8715573970539126 1 53 -3.9618324181102835 1 54 -4.295303535854799 1 55 -3.8437734685220235 1 56 -3.8628990562533936 1 57 -4.335904809555007 1 58 -4.130423187532553 2 1 -0.5696058338064702 2 2 -1.367227542852163 2 3 -1.132234667913501 2 4 -0.8918141519473863 2 5 -1.4444158942672871 2 6 -2.768625686033552 2 7 -2.370834827463824 2 8 -3.068830423627041 2 9 -2.9067097454487083 2 10 -1.2110274803509 2 11 -1.4266873139038978 2 12 -1.6103406044606032 2 13 -1.6387493617472515 2 14 -1.3844462172556007 2 15 -1.599187310494841 2 16 -1.5636085343531647 2 17 -1.4780139058507515 2 18 -1.7565571421695196 2 19 -1.5508265152871488 2 20 -1.5240078541756361 2 21 -1.4379383725558554 2 22 -1.4404036466597319 2 23 -1.2368681509517074 2 24 -1.2041923199106819 2 25 -1.890603227035065 2 26 -1.582365342655443 2 27 -1.6000259580783762 2 28 -1.8607631196811987 2 29 -1.5527246461793638 2 30 -2.079991483497057 2 31 -2.287872806506069 2 32 -2.193077383494824 2 33 -2.3942349950670945 2 34 -3.0401839629360197 2 35 -2.5558899966156012 2 36 -2.892464812523354 2 37 -2.655121350105997 2 38 -2.75815030262158 2 39 -2.498302963434341 2 40 -2.85640371798279 2 41 -2.585368199058852 2 42 -2.6573424614419388 2 43 -2.6850466539297635 2 44 -2.0786995592533017 2 45 -2.1654055276029354 2 46 -2.278373588815917 2 47 -2.0760187360531823 2 48 -2.3573189118271323 2 49 -2.1801596134834007 2 50 -1.888667895789503 2 51 -1.801296873056625 2 52 -1.7448351794151016 2 53 -1.6218439683124117 2 54 -1.8615286233652597 2 55 -2.2176759046718004 2 56 -1.6201621297857507 2 57 -1.5657066882307404 2 58 -1.87012523946132 3 1 -0.05253165619156874 3 2 -0.3172072638713246 3 3 -0.3281296227927655 3 4 -0.49929999287326865 3 5 -0.2635744614124391 3 6 -0.39196534447757614 3 7 -0.7816906004551143 3 8 -0.9120992504879979 3 9 -0.9247833871191204 3 10 -0.1096108486896199 3 11 0.05514489201466688 3 12 -0.30434164737961567 3 13 -0.297675010312517 3 14 0.17498206033467767 3 15 -0.43574234620032826 3 16 -0.010676773123677208 3 17 0.1747918359978229 3 18 -0.0179314776707713 3 19 -0.10944363364396992 3 20 0.05742673886867067 3 21 -0.3448650930101067 3 22 -0.21449217889607297 3 23 -0.4385788278351228 3 24 -0.31432167083844575 3 25 -0.7021318276405353 3 26 -0.24158069094693957 3 27 0.22481796619411654 3 28 0.17837531333297818 3 29 0.1036516447950433 3 30 -0.29133105503115786 3 31 -0.31146522796122866 3 32 -0.7245279100240758 3 33 -1.098538484500299 3 34 -0.8816638939874863 3 35 -0.5099870749938624 3 36 -0.6984166933988263 3 37 -0.7888612287218102 3 38 -0.7688981624648505 3 39 -0.3770817342384021 3 40 -0.8257027894951676 3 41 -0.3848271848201108 3 42 -0.32575215479478103 3 43 -0.468764080816797 3 44 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0.2997842886009394 118 28 0.07756705109678563 118 29 0.0649224126441652 118 30 -0.016993381030260934 118 31 -0.2651024080240951 118 32 -0.3663854163854019 118 33 -0.8739313897540022 118 34 -0.9303167902604983 118 35 -0.5696787379185757 118 36 -1.6293402078427732 118 37 -1.3324774538378108 118 38 -1.1651711754868543 118 39 -0.8049443296582517 118 40 -1.3972639330365553 118 41 -1.0975124115825707 118 42 -1.0319787675772965 118 43 -1.0153439341321686 118 44 -0.6804705902463439 118 45 -0.7839381019229883 118 46 -0.5714792181644306 118 47 -0.690692992852675 118 48 -0.7466294747619848 118 49 -1.0211463307509518 118 50 -0.8013181171683783 118 51 -1.0532260318169016 118 52 -1.204038215468234 118 53 -1.3115340378886273 118 54 -1.3825279908541894 118 55 -1.4326664170057661 118 56 -1.8796814213733555 118 57 -1.7836700025887424 118 58 -1.9459633166276689 119 1 -0.0315543165269645 119 2 -0.289798476229322 119 3 -0.2544985595001066 119 4 0.11072585313262095 119 5 -0.23676674367487038 119 6 -0.7817291240051318 119 7 -1.0942469507586594 119 8 -1.0134396363748763 119 9 -1.1934165541564496 119 10 0.2789357642814707 119 11 0.410662713737338 119 12 0.2922352595146549 119 13 0.043492267441891386 119 14 0.5694041316215674 119 15 -0.03733818679658896 119 16 -0.20337978448390004 119 17 -0.2756950306247674 119 18 -0.21472176438111745 119 19 -0.8130260102743705 119 20 -0.5825624418730513 119 21 -1.1442129321638035 119 22 -1.175197233893968 119 23 -1.009985932481392 119 24 -1.0266646413259153 119 25 -1.0537126414891844 119 26 -1.3344309644337429 119 27 -1.387987828119762 119 28 -1.2789637502001057 119 29 -1.063956698055236 119 30 -1.1961967159075222 119 31 -1.5299240847262716 119 32 -1.5934195371203081 119 33 -1.5773357436215356 119 34 -2.0800620426921803 119 35 -1.727799781611644 119 36 -1.9062816930982933 119 37 -1.6718629718245346 119 38 -1.9727209670570254 119 39 -1.5305348808393546 119 40 -2.0475727179678493 119 41 -1.878290549609435 119 42 -1.9189480434766324 119 43 -1.7570221802337578 119 44 -1.7373564942142288 119 45 -1.8333745694233734 119 46 -1.9027591317046486 119 47 -1.8372731997385792 119 48 -1.6085254142401375 119 49 -2.1189230792456257 119 50 -2.1639782993514878 119 51 -2.1445337360009376 119 52 -2.136877492911495 119 53 -2.015324372920279 119 54 -1.7946555190308153 119 55 -1.4270619705027556 119 56 -1.809456008373509 119 57 -1.78479706758797 119 58 -1.5257379036278245 120 1 -0.358192580686449 120 2 -1.1787093721209274 120 3 -0.9200226585154496 120 4 -0.7254234783036182 120 5 -1.2025018404428067 120 6 -1.179439677126446 120 7 -1.468396088173975 120 8 -1.6231867624399712 120 9 -1.4384164733143976 120 10 -0.5151393218153117 120 11 -0.5175818819715658 120 12 -0.7871112133466696 120 13 -0.9023233480086432 120 14 -0.9172205510733775 120 15 -1.0674922717454223 120 16 -0.8466682774263887 120 17 -0.633518115880797 120 18 -0.7965339197918766 120 19 -0.8921097464705952 120 20 -0.8054116391114832 120 21 -0.7282765386893715 120 22 -1.0782514465732334 120 23 -1.1436825160963267 120 24 -0.9182999143295163 120 25 -1.0518896532436202 120 26 -0.6050578136956588 120 27 -0.9978845119788389 120 28 -0.5997490498815878 120 29 -0.7932126278196563 120 30 -1.0496543304202852 120 31 -1.6400269158148895 120 32 -1.0960226511561046 120 33 -1.2266793061690286 120 34 -1.3970760607037889 120 35 -1.3874264530210425 120 36 -0.8744986408258235 120 37 -1.1049419221713457 120 38 -1.198448141303981 120 39 -0.8304063419785805 120 40 -0.8245820471405132 120 41 -0.6843828065578101 120 42 -0.6257662620412034 120 43 -0.7163384181680819 120 44 -0.5569129947235361 120 45 -0.7250219687345982 120 46 -0.4777158812074731 120 47 -0.6687660290486726 120 48 -0.772699451007103 120 49 -1.0605955395925974 120 50 -0.6958987831103194 120 51 -0.8908432963818669 120 52 -0.7741553793769178 120 53 -0.8516636831759001 120 54 -0.8356060598641335 120 55 -0.6272430566965497 120 56 -0.6204270830877334 120 57 -0.3249108931955007 120 58 -0.29820795164800273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2616.0 3 327.0 4 783.0 5 512.0 6 1175.0 7 2531.0 8 1593.0 9 2822.0 10 3219.0 11 3144.0 12 4530.0 13 6100.0 14 6727.0 15 7201.0 16 8004.0 17 8029.0 18 9724.0 19 9442.0 20 10488.0 21 8833.0 22 11181.0 23 9595.0 24 7140.0 25 7545.0 26 7731.0 27 8825.0 28 10135.0 29 12777.0 30 15385.0 31 18782.0 32 22998.0 33 27709.0 34 32611.0 35 39295.0 36 48110.0 37 66652.0 38 80028.0 39 76180.0 40 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 38.83368547535817 46.37014014637249 5.128239552793613 9.667934825475728 11 27.72121195967242 49.14540450853263 13.709011068094433 9.424372463700514 12 59.67566613787352 13.930342461534876 17.503441594436186 8.89054980615542 13 23.273168547405778 15.987308964458775 24.333196279915235 36.40632620822021 14 27.587188446230392 37.07733308475061 18.060422246599607 17.275056222419387 15 40.73815750267587 21.162934319230146 17.906278766652406 20.192629411441573 16 26.14796109458517 23.428144335560035 38.07527062501352 12.34862394484127 17 49.262226425965366 16.481466987942177 18.919341973579694 15.33696461251276 18 43.13893910490097 16.291461502352107 25.325349819721744 15.24424957302518 19 28.26019286245774 16.19005924369818 38.59979125710167 16.94995663674241 20 17.082102894126187 36.7418857597539 24.84760309887571 21.328408247244205 21 19.10213919855378 21.518163462002633 47.00460599361786 12.375091345825725 22 19.81327766290758 23.706378459007986 23.84671987320994 32.63362400487449 23 24.182629004040038 35.000840634087794 27.422815641453745 13.393714720418421 24 27.259591935932242 35.75695934507332 20.244898366844836 16.738550352149602 25 19.22258589746578 14.039103782851473 26.789787200037292 39.94852311964546 26 40.605213811571375 12.700797026388802 32.458091999427126 14.235897162612702 27 26.187577952105563 13.348064789543884 26.82768026190242 33.63667699644813 28 36.02267030626231 32.49454976658511 20.01555544082354 11.467224486329032 29 54.9316292310872 15.675273849764318 18.967804734950764 10.425292184197717 30 38.19902810620791 34.18171145360491 16.69762896603708 10.921631474150093 31 51.57982709221582 20.261808955710904 17.857648247697977 10.300715704375303 32 32.35519371326774 24.45698683049465 32.6373973993973 10.550422056840318 33 38.78438228438228 27.62703962703963 19.508991008991007 14.07958707958708 34 23.34860147486584 32.6748218855572 21.821787321614995 22.154789317961963 35 19.068259476597415 51.16829744293393 20.190764189085677 9.572678891382974 36 27.38045464464126 40.68464878893022 19.48922022130903 12.445676345119491 37 18.280759147042264 40.9528981041312 30.601907528507986 10.164435220318543 38 26.225834827170285 39.29352063494179 23.720020524986506 10.760624012901419 39 18.403631990739893 38.38508508725551 23.541557906534948 19.669725015469652 40 17.684209824857184 38.424630947283894 31.581349864140623 12.3098093637183 41 14.990828164283323 46.31252217984795 26.018357000168272 12.67829265570045 42 15.915710267791686 39.67355807040695 31.9682155663081 12.44251609549326 43 24.2043640411541 37.31531813773359 25.185223451305312 13.295094369806995 44 16.405069119811007 38.57229134759441 26.0089356917153 19.013703840879288 45 17.098180509217443 37.9749957504108 33.11396564998717 11.812858090384596 46 16.637054864613507 38.1782241585637 33.07877183800955 12.105949138813239 47 23.924342236861406 37.79104268626065 26.513388630332447 11.771226446545494 48 25.48740481937351 37.45382656122898 25.100514742162183 11.958253877235329 49 27.185230014121114 38.19103017450497 23.662787111273385 10.960952700100531 50 17.16219978361582 42.88807869849415 27.067194123952465 12.882527393937576 51 16.41425720254838 45.027369600485535 26.225963021443828 12.332410175522257 52 21.154763373902256 39.89490325829917 27.307308742941576 11.643024624856997 53 15.823715565277997 40.601209154358926 26.706104795600893 16.868970484762187 54 14.803587596480433 45.09006507896014 25.967889043957893 14.138458280601535 55 20.130027169607402 41.27078374893882 24.174279104753932 14.424909976699851 56 17.05149643039521 42.21962998935293 26.986312342922368 13.74256123732949 57 22.381175591390495 41.568900891944146 23.694153403716907 12.355770112948454 58 21.787461538974952 42.22152882991717 23.381967202173172 12.609042428934705 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 838.0 9 2255.5 10 2835.0 11 2455.0 12 2075.0 13 1658.5 14 1242.0 15 1465.0 16 1824.5 17 1961.0 18 2078.0 19 2195.0 20 2531.0 21 2867.0 22 3728.5 23 5985.5 24 7381.0 25 8765.5 26 10150.0 27 13616.0 28 22546.0 29 28010.0 30 32057.0 31 36104.0 32 42863.0 33 49622.0 34 39634.0 35 25980.5 36 22315.0 37 20239.0 38 18163.0 39 16945.5 40 15728.0 41 15279.5 42 15421.5 43 16012.0 44 16717.5 45 17423.0 46 17062.0 47 18179.0 48 19657.0 49 21542.0 50 23427.0 51 26867.5 52 30308.0 53 37523.0 54 43568.5 55 42399.0 56 38376.0 57 34353.0 58 31295.5 59 24829.5 60 21421.0 61 18990.0 62 16559.0 63 12521.5 64 8484.0 65 6430.5 66 3448.0 67 2519.0 68 1963.5 69 1408.0 70 1291.0 71 1174.0 72 871.5 73 495.0 74 421.0 75 306.5 76 192.0 77 136.0 78 73.0 79 66.0 80 45.0 81 24.0 82 20.5 83 17.0 84 10.0 85 3.0 86 3.0 87 3.0 88 3.0 89 2.0 90 1.0 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.8093365005434972 11 0.07574004684230588 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.035955714544918846 18 1.664616414116613E-4 19 0.0 20 8.323082070583065E-4 21 0.0 22 0.010154160126111339 23 3.329232828233226E-4 24 0.0 25 0.013483392954344565 26 0.04278064184279695 27 0.034956944696448874 28 0.050937262271968355 29 0.1290077720940375 30 0.1806108809316525 31 0.011985238181639615 32 0.018144318913871083 33 0.023138168156220922 34 0.023637553080455904 35 0.03445755977221389 36 0.06691757984748785 37 0.06342188537784295 38 0.08239851249877235 39 0.196091813582937 40 0.08090035772606739 41 0.08855759323100382 42 0.0950495972460586 43 0.10869945184181483 44 0.22389090769868447 45 0.11336037780134134 46 0.12534561598298097 47 0.1376637774474439 48 0.1376637774474439 49 0.15447640323002168 50 0.14648624444226196 51 0.16413117843189806 52 0.1832742671942391 53 0.16329887022483974 54 0.16746041126013128 55 0.19509304373446704 56 0.25185646345584356 57 0.21007459146151655 58 0.23820660886008732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 600739.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.38924760656844 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57918909834393 38.659763917244895 2 9.138251737626984 8.661097485711188 3 3.5455422669180274 5.040617411595971 4 1.713838052571091 3.2486998332340193 5 0.9347834733260436 2.2149342737987974 6 0.5820959701826038 1.6551054037061455 7 0.39760334247707935 1.3189486272091882 8 0.3087002747671497 1.170325900572493 9 0.22921583069447798 0.9776129180513299 >10 1.3561261268461033 11.806050413875482 >50 0.11870741803538488 3.8762539374183875 >100 0.07633692332126832 7.161918021218376 >500 0.009104403698866862 2.977342689513198 >1k 0.009804742444933545 8.25116545260644 >5k 3.5016937303334087E-4 0.9573230395608984 >10k+ 3.5016937303334087E-4 2.0228406746831946 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 12190 2.0291674088081515 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGTGG 5769 0.960317209303874 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 4449 0.740587842640481 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3179 0.5291815580476713 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3156 0.5253529402952031 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 3143 0.5231889389568515 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 2303 0.383361160171056 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 2225 0.3703771521409464 No Hit ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 2219 0.36937838229247644 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 1888 0.3142795789852165 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1885 0.31378019406098157 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1865 0.31045096123274835 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 1813 0.3017949558793419 Illumina Single End Adapter 2 (96% over 25bp) ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 1682 0.2799884808544143 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA 1675 0.2788232493645327 No Hit ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1606 0.26733739610712803 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1408 0.23437799110761914 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1403 0.23354568290056082 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1370 0.228052448733976 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 1297 0.2159007489109247 No Hit ATGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1248 0.2077441284817533 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1220 0.2030832025222268 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA 1150 0.1914308876234105 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1145 0.1905985794163522 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA 1092 0.18177611242153413 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 1076 0.17911272615894755 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAA 1072 0.1784468795933009 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1063 0.17694872482059595 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 1054 0.175450570047891 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 1037 0.17262072214389276 No Hit ATGCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 997 0.16596225648742632 No Hit ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 904 0.15048132383614182 No Hit ATGCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 881 0.14665270608367362 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 880 0.14648624444226196 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 835 0.13899547057873718 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 822 0.13683146924038558 No Hit ATGCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 791 0.13167115835662407 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA 776 0.12917423373544917 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 711 0.1183542270436912 Illumina Single End Adapter 2 (96% over 32bp) ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 686 0.11419268600839966 No Hit ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 682 0.11352683944275302 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 673 0.11202868467004806 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGGGG 665 0.11069699153875476 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 648 0.10786714363475652 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 640 0.10653545050346323 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA 637 0.10603606557922825 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 632 0.10520375737216996 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 627 0.10437144916511164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.03412463648939057 0.0 0.0 0.0 0.0 11 0.06808281133736947 0.0 0.0 0.0 0.0 12 0.07424189206960094 0.0 0.0 0.0 0.0 13 0.07890281802912746 0.0 0.0 0.0 0.0 14 0.10653545050346323 0.0 0.0 0.0 0.0 15 0.11302745451851802 0.0 0.0 0.0 0.0 16 0.12085115166486611 0.0 0.0 0.0 0.0 17 0.12534561598298097 0.0 0.0 0.0 0.0 18 0.13083885014956578 0.0 0.0 0.0 0.0 19 0.13666500759897393 0.0 0.0 0.0 0.0 20 0.16762687290154293 0.0 0.0 0.0 0.0 21 0.17927918780035923 0.0 0.0 0.0 0.0 22 0.1922631958304688 0.0 0.0 0.0 0.0 23 0.2112398229513982 0.0 0.0 0.0 0.0 24 0.23254691305209085 0.0 0.0 0.0 0.0 25 0.24236814989537886 0.0 0.0 0.0 0.0 26 0.2520229250972552 0.0 0.0 0.0 0.0 27 0.26167770029913157 0.0 0.0 0.0 0.0 28 0.40583348176163025 0.0 0.0 0.0 0.0 29 0.4181516432260932 0.0 0.0 0.0 0.0 30 0.47424921638182305 0.0 0.0 0.0 0.0 31 0.48856491754322595 0.0 0.0 0.0 0.0 32 0.5017153872147472 0.0 0.0 0.0 0.0 33 0.5173627815074433 0.0 0.0 0.0 0.0 34 0.5355071004213144 0.0 0.0 0.0 0.0 35 0.7162844429943785 0.0 0.0 0.0 0.0 36 0.732764145494133 0.0 0.0 0.0 0.0 37 0.7460810768070659 0.0 0.0 0.0 0.0 38 0.7569010834988239 0.0 0.0 0.0 0.0 39 0.7742130942056368 0.0 0.0 0.0 0.0 40 0.7926903364023311 0.0 0.0 0.0 0.0 41 0.8115005018818489 0.0 0.0 0.0 0.0 42 0.837468517942068 0.0 0.0 0.0 0.0 43 0.8670986901133437 0.0 0.0 0.0 0.0 44 0.9018891731683809 0.0 0.0 0.0 0.0 45 0.940175350693063 0.0 0.0 0.0 0.0 46 0.9866181486469165 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAATC 70 0.0 52.32152 10 GAACACG 15 0.007044628 52.32152 9 CATCGGG 15 0.0072002895 52.03315 27 CGCCAGC 15 0.0072002895 52.03315 27 ACTCACT 20 3.4383388E-4 52.024467 28 TGATCGA 15 0.0072050462 52.024464 29 GTAGACA 15 0.0072288644 51.981064 42 TCCGAAT 20 3.453957E-4 51.97673 41 ACCGACC 15 0.0072312495 51.976723 41 GTGATGT 15 0.0072312495 51.976723 36 ATAACGG 15 0.0072312495 51.976723 36 AGTTCCG 15 0.0072360216 51.968052 30 CAGCGTT 15 0.0072407955 51.959385 34 ACCGGCA 15 0.0072407955 51.959385 33 CCGGCAC 15 0.0072407955 51.959385 34 AATCAAT 25 1.6643708E-5 51.946396 45 GTATTAT 20 3.463925E-4 51.946392 49 TTATCCC 15 0.007247961 51.946392 46 GTCGTCG 15 0.007247961 51.946392 49 TTCGGTC 20 3.463925E-4 51.946392 45 >>END_MODULE