FastQCFastQC Report
Tue 31 May 2016
SRR212588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525941
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT126572.406543699768605No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA46310.8805170161672127No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGT37280.7088247541073999No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA33470.6363831684542562No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA32790.6234539615660312No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA32530.6185104412852392No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC26090.4960632466379309No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA22870.4348396493142767No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA21940.41715705754067467No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20900.397382976417507No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA20360.3871156650650929No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC19970.37970038464390493No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA19340.3677218547327552No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAA17090.3249413907643633No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA16980.32284990141479747No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAA16060.3053574450366106No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15460.29394932131170604No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA14510.27588645874727397No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG14420.2741752401885383Illumina Single End Adapter 2 (95% over 23bp)
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAA12670.2409015459909001No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA12490.23747910887342874No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAA12450.23671856729176846No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAA11620.22093732947231723No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAA11370.21618394458694035No Hit
ATGCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11030.2097193411428278No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA11000.20914893495658257No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10950.20819825797950722No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAA10620.20192378993080973No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAA10410.19793094662709315No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAA10380.19736054044084791No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGG9490.18043849024890624No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT8970.17055144968732233No Hit
ATGCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA8910.1694106373148319No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAA8710.1656079294065304No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA8610.16370657545237963No Hit
ATGCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8460.1608545445211535No Hit
ATGCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8340.1585729197761726No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA7980.1517280455412299No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA7470.1420311403750611No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAA7450.14165086958423093No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG7330.13936924483925003Illumina Single End Adapter 2 (96% over 30bp)
ATGCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7000.13309477679055254No Hit
ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6990.1329046413951375No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAA6910.13138355823181685No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAA6900.1311934228364018No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAA6660.12663017334644No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA6630.12605976716019476No Hit
ATGCTTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC6460.1228274654381385No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAA6430.12225705925189327No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAA6270.11921489292525207No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAA5960.11332069566738474No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA5770.10970812315449832No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAA5490.10438433208287623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT204.05999E-450.30041550
TCGTCGG204.05999E-450.30041550
GATATGG204.05999E-450.30041550
ACCCCGC204.05999E-450.30041550
TTTTACG150.00823122350.3004150
GTCGTCG204.061913E-450.2955949
GTAGCCG252.0144233E-550.2955849
CCCCTAG204.0734682E-450.26664448
GGTCGTC355.0653398E-850.26664448
TAGCTGC150.00825623350.2618247
CACCTAA150.008262495550.25218246
GCGTGAC150.008262495550.25218246
TTTCGTT150.00826562850.24736445
TGTACTC150.00827189450.2377343
ACCAGAC150.00828443950.21847541
ACATCCT301.0204785E-650.18481439
TACATCC301.0211515E-650.18000838
CGCTTTG355.1462848E-850.165637
ACCTCTC150.0083190150.16559637
ATCGGAT150.00832530750.15599436
Produced by FastQC (version 0.11.5)