FastQCFastQC Report
Tue 31 May 2016
SRR212587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372367
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT112403.018527420528672No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT45341.2176159541527578No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA42981.1542376204121203No Hit
ACAATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC38401.0312406845934252No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG29280.7863210220024869No Hit
ACAATAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG20540.5516063453528374No Hit
ACAATAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC14340.38510394315285723No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.3716763300722137No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAGCAGTGG12800.34374689486447507No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA11790.31662311644157515No Hit
ACAATAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT10850.29137920384996524No Hit
ACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10230.27472896362996724No Hit
ACAATAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC9970.2677466048280326No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9380.25190202139287315No Hit
ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA9230.2478737374686801No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA9020.2422341399748098No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8710.23390901986481077No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.23041784046384348No Hit
ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.22209272035384445No Hit
ACAATAGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8240.22128706356900585No Hit
ACAATAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG8080.21699022738319992No Hit
ACAATAGGGCAGGACCATGTACACATACATGTGTGCCTGTGGTTTAAAAGGCAAAAAT7960.21376760024384547No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGA7560.2030255097793306No Hit
ACAATAGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC6420.1724105519554633No Hit
ACAATAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6300.16918792481610884No Hit
ACAATAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC6290.16891937255449596No Hit
ACAATAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6250.1678451635080445No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC6220.1670395067232059No Hit
ACAATAGGGGAGTGCAGTGCTACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGA6090.16354832732223856No Hit
ACAATAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5840.15683452078191676No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGTTCTTCCGAA5760.1546861026890138No Hit
ACAATAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5550.1490465051951435No Hit
ACAATAGGGACCTAGAAAGGCAAGTTCTAGAGTTCTCTTTTTCCCTTATACCCAAGGC5480.1471666393638534No Hit
ACAATAGGGATGATCGGAAGGACGGACGGAACCGGGCTTGTGTGGAGAGAGTCTGATG5460.14662953484062766No Hit
ACAATAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA5420.14555532579417618No Hit
ACAATAGGGACGCCATGATGTCGATGCTCTCCTCTGGCAACCACACTGCAGCAAGCAG5380.1444811167477247No Hit
ACAATAGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCC5250.14098993734675738No Hit
ACAATAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5250.14098993734675738No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5130.1377673102074029No Hit
ACAATAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4810.12917363783579103No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC4720.1267566674812752Illumina Single End Adapter 2 (96% over 25bp)
ACAATAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC4610.12380259260353361No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4480.12031141320256629No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4270.11467181570869599No Hit
ACAATAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4190.11252339761579302No Hit
ACAATAGGGAAGCACAAGATGTGTTAGACTGAAAAAAGAACTGATCTCTTAGAACCGT4110.11037497952289005No Hit
ACAATAGGGATGCCGTTGTGAAAGTCCTACAAGTAAATCTCTATATTCATGATAATCT4010.10768945690676135No Hit
ACAATAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC4000.10742090464514846No Hit
ACAATAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3950.10607814333708412No Hit
ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC3790.10178130715127819No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3760.10097565036643957No Hit
ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA3750.10070709810482668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC150.00718273252.06137545
CCCTAAC251.6415153E-552.06137550
CCTAACG251.6415153E-552.06137551
CGTTACC150.00718273252.06137546
TAGCACC150.00718273252.06137545
GTTACAT150.00718273252.06137552
ACTAGGT150.00718273252.06137546
CGAATGA150.00718273252.06137547
CGGGACA307.915751E-752.06137546
CGCGTAG150.00718273252.06137546
CAATATG150.00718273252.06137545
GCGTAGG150.00718273252.06137547
GTCGGAC150.00718273252.06137551
AACGTCA203.4246282E-452.0613749
TATTATC203.4246282E-452.0613744
TATAGCC203.4246282E-452.0613747
CCGTAGG203.4269047E-452.0543742
AGGTTAG203.4269047E-452.0543748
CCGACTA203.4269047E-452.0543742
CGACTAC203.4269047E-452.0543743
Produced by FastQC (version 0.11.5)