##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212587_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 372367 Sequences flagged as poor quality 0 Sequence length 58 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.85409287074311 39.0 38.0 40.0 34.0 40.0 2 37.70116041432243 39.0 38.0 40.0 33.0 40.0 3 37.43714668593081 39.0 38.0 40.0 33.0 40.0 4 37.69777128478088 39.0 38.0 40.0 33.0 40.0 5 37.49834974635239 39.0 38.0 40.0 33.0 40.0 6 37.65158029578346 39.0 38.0 40.0 33.0 40.0 7 37.54631049475383 39.0 38.0 40.0 33.0 40.0 8 37.30519621770995 39.0 38.0 40.0 33.0 40.0 9 37.18916015651226 39.0 38.0 40.0 32.0 40.0 10 36.815727494649096 39.0 36.0 40.0 31.0 40.0 11 37.31021008843426 39.0 37.0 40.0 33.0 40.0 12 37.16643257861196 39.0 36.0 40.0 32.0 40.0 13 37.12426450249351 39.0 36.0 40.0 32.0 40.0 14 37.093617318398245 39.0 36.0 40.0 33.0 40.0 15 37.03456267606958 39.0 36.0 40.0 33.0 40.0 16 36.85555379504628 39.0 36.0 40.0 32.0 40.0 17 36.698678991425126 38.0 36.0 40.0 31.0 40.0 18 36.547239685579015 38.0 36.0 40.0 31.0 40.0 19 36.29370486643553 38.0 35.0 40.0 30.0 40.0 20 36.408266575716965 38.0 36.0 40.0 31.0 40.0 21 36.361927882975664 38.0 36.0 40.0 31.0 40.0 22 36.2507741018941 38.0 36.0 40.0 31.0 40.0 23 36.03147701058364 38.0 35.0 40.0 30.0 40.0 24 35.878190065177634 38.0 35.0 40.0 29.0 40.0 25 35.90895272674539 38.0 35.0 40.0 30.0 40.0 26 35.660872204035265 38.0 35.0 40.0 29.0 40.0 27 35.62264916063991 38.0 35.0 40.0 29.0 40.0 28 35.09298353506084 38.0 35.0 39.0 28.0 40.0 29 35.2080662357298 38.0 35.0 39.0 28.0 40.0 30 34.76770497922748 38.0 35.0 39.0 27.0 40.0 31 34.25324478270094 38.0 34.0 39.0 25.0 40.0 32 33.828244715562874 38.0 33.0 39.0 23.0 40.0 33 33.830092355122765 38.0 33.0 39.0 23.0 40.0 34 33.38652995566202 38.0 33.0 39.0 21.0 40.0 35 33.06156292045213 38.0 32.0 39.0 19.0 40.0 36 33.42393122913685 38.0 33.0 39.0 16.0 40.0 37 33.339681013623654 38.0 33.0 39.0 17.0 40.0 38 33.16782905037235 38.0 33.0 39.0 15.0 40.0 39 33.15568780262483 38.0 33.0 39.0 15.0 40.0 40 33.02378298828844 38.0 33.0 39.0 14.0 40.0 41 33.254509663853135 38.0 34.0 40.0 10.0 40.0 42 33.13464404740485 38.0 33.0 40.0 9.0 40.0 43 32.940295461198225 38.0 33.0 39.0 7.0 40.0 44 32.91198736730161 38.0 33.0 39.0 2.0 40.0 45 32.688675419680045 38.0 33.0 39.0 2.0 40.0 46 32.27068993761531 38.0 33.0 39.0 2.0 40.0 47 32.11483563258828 38.0 32.0 39.0 2.0 40.0 48 32.10974119618548 38.0 32.0 39.0 2.0 40.0 49 31.92840396705401 38.0 32.0 39.0 2.0 40.0 50 31.703378656003352 38.0 32.0 39.0 2.0 40.0 51 31.452881162938713 38.0 32.0 39.0 2.0 40.0 52 31.30648526856569 38.0 31.0 39.0 2.0 40.0 53 31.156523000158447 37.0 31.0 39.0 2.0 40.0 54 30.91957933973741 37.0 31.0 39.0 2.0 40.0 55 30.664508401657503 37.0 30.0 39.0 2.0 40.0 56 30.402664575539724 38.0 30.0 39.0 2.0 40.0 57 30.099557694425123 37.0 30.0 39.0 2.0 40.0 58 29.735406198723304 36.0 29.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -1.0837485941408715 1 2 -1.6705700910469758 1 3 -1.4093808914734112 1 4 -1.496162045446816 1 5 -2.521353127014031 1 6 -2.0702216735846974 1 7 -2.6678969580125695 1 8 -2.921219429945957 1 9 -2.7800350986127498 1 10 -2.323681287693482 1 11 -1.6283429632185076 1 12 -1.9232540978850992 1 13 -1.9489493120397796 1 14 -1.439038597564135 1 15 -1.718920816853263 1 16 -1.8428681465311314 1 17 -2.016902649654476 1 18 -2.0284692784578766 1 19 -1.8363157252499391 1 20 -1.532950061819676 1 21 -2.2065028744708712 1 22 -1.8085535371468708 1 23 -1.4645415386146823 1 24 -1.8726282280837836 1 25 -1.5622019297103051 1 26 -1.5325681693738389 1 27 -1.4095787760065903 1 28 -1.0829772430990516 1 29 -1.663201102884905 1 30 -1.9135864535160536 1 31 -1.8694086568370665 1 32 -2.015870533567881 1 33 -2.278123025951899 1 34 -2.2639792703665087 1 35 -2.387212061709299 1 36 -1.8296638790157402 1 37 -2.1275263992065625 1 38 -1.9258006091514268 1 39 -1.9918049287984552 1 40 -2.1556855261912133 1 41 -2.0541655597796797 1 42 -1.8433252754965466 1 43 -2.1812998372731087 1 44 -2.1934053734408643 1 45 -1.7547827392795803 1 46 -1.5450979748590186 1 47 -1.442102990687527 1 48 -2.0506999489858657 1 49 -1.8476564968834985 1 50 -1.4757470919074223 1 51 -1.43774621965823 1 52 -1.5210301718752248 1 53 -1.322142318314377 1 54 -1.378282175464843 1 55 -1.3677591674594076 1 56 -1.4339143648032682 1 57 -1.2618674174686895 1 58 -1.7526496233574989 2 1 -0.35809707392704127 2 2 -0.6541150292360811 2 3 -0.490904446434989 2 4 -0.6753301042506905 2 5 -0.6120647875552905 2 6 -0.9431752318874018 2 7 -0.9977081842070277 2 8 -1.6183878228627648 2 9 -1.5945188941356392 2 10 -0.894554993005876 2 11 -0.6684365148389588 2 12 -0.44989960406618934 2 13 -0.5435450353942741 2 14 -0.7424966663914034 2 15 -0.8502689625264992 2 16 -0.6629483336343682 2 17 -0.8496791281041922 2 18 -0.5166082694368299 2 19 -0.6896401489051343 2 20 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0.01515972540203947 117 56 -0.03748765850029301 117 57 -0.30374666062941813 117 58 -0.4378623337580123 118 1 -0.9725828045614691 118 2 -1.24294987652884 118 3 -1.468052003834316 118 4 -1.3306837985500977 118 5 -1.6464686213297028 118 6 -1.4046229108803203 118 7 -2.0954569146268796 118 8 -1.9357917996884595 118 9 -2.363562596302863 118 10 -1.4375687259291325 118 11 -1.3152428949262145 118 12 -0.9269398730026026 118 13 -1.4726005392924222 118 14 -1.3698123090492942 118 15 -1.1014580763237944 118 16 -1.0755439991804678 118 17 -1.472131027109988 118 18 -1.2806988208995236 118 19 -1.161798792778967 118 20 -1.368626758079671 118 21 -1.4774224100832924 118 22 -1.3515174225765136 118 23 -1.1869835905971655 118 24 -1.7929773222066316 118 25 -0.8142908703763609 118 26 -1.0024090885077328 118 27 -1.5647127494127702 118 28 -1.1255092802179192 118 29 -1.2087058632593042 118 30 -1.4755114926836868 118 31 -1.15377574798719 118 32 -1.155488096738253 118 33 -1.0118031769983773 118 34 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-0.23400986395560608 119 14 -0.5350382005023917 119 15 -0.04294305231087492 119 16 -0.003598740138436085 119 17 -0.05176467069875201 119 18 0.21172329648752708 119 19 -0.08520395236925538 119 20 -0.1432538140531534 119 21 -0.06153563206468249 119 22 -0.12147789688481225 119 23 0.09897841373791039 119 24 -0.025983399812986363 119 25 0.053710319644466153 119 26 0.2390121363636979 119 27 -0.0464161792227884 119 28 -0.10484541860034113 119 29 -0.1537014006812143 119 30 -0.009278333603404576 119 31 -0.4595515990645822 119 32 -0.07942026555120663 119 33 -0.37494653200708683 119 34 -0.1711859410037917 119 35 -0.22444652666860065 119 36 0.10090987520304395 119 37 0.08911048782937314 119 38 -0.11627112731699896 119 39 -0.006009812657893576 119 40 -0.016604724126487724 119 41 -0.3492519207088023 119 42 -0.1531426270947236 119 43 -0.11023571180160019 119 44 -0.41457869089173016 119 45 -0.1598254234169616 119 46 -0.4218599363440312 119 47 -0.10385601828331659 119 48 -0.05490880346273741 119 49 0.09834310605475594 119 50 0.2718606110903963 119 51 0.18965104061574323 119 52 0.6786422521867159 119 53 0.3600196828768105 119 54 0.7146104193336491 119 55 0.6278334471921703 119 56 0.5787221251709198 119 57 0.3478407421501295 119 58 0.9935882166266197 120 1 -2.0604341106426034 120 2 -2.704414482408552 120 3 -2.77868328507747 120 4 -2.8862926943036413 120 5 -3.660411285255144 120 6 -3.1110860035218053 120 7 -4.234330439219612 120 8 -4.926618445204575 120 9 -4.929732851257413 120 10 -3.036936296712156 120 11 -2.6187880468240294 120 12 -2.585261117823677 120 13 -2.695756214979383 120 14 -2.3803319237475336 120 15 -2.776957184035048 120 16 -3.0126141612535022 120 17 -2.92193848059096 120 18 -2.787049203383532 120 19 -2.800155729149907 120 20 -2.754053752162392 120 21 -2.7023835355150894 120 22 -2.417191961560661 120 23 -2.9088817131381504 120 24 -2.590819131413376 120 25 -2.9918928574146904 120 26 -2.1744903650546306 120 27 -2.8322741399221556 120 28 -2.374390005737375 120 29 -2.744820076218886 120 30 -3.221976255105286 120 31 -2.8214268891775376 120 32 -3.2823600767843786 120 33 -3.270639966428668 120 34 -3.5712478317665948 120 35 -3.2674709219442732 120 36 -3.179125196229215 120 37 -2.969139035095999 120 38 -3.111536483962521 120 39 -3.247971749838772 120 40 -3.0491798956154774 120 41 -3.1729560828626013 120 42 -3.563344287830187 120 43 -3.389228955393328 120 44 -3.225007284424141 120 45 -3.244130957482671 120 46 -3.1800733563244314 120 47 -2.6302885315640445 120 48 -2.537171942355922 120 49 -2.5389254482801746 120 50 -2.5117486518937717 120 51 -1.7662208455052557 120 52 -1.9753883837408708 120 53 -1.9205311449121432 120 54 -1.8843271225715554 120 55 -2.111482659878849 120 56 -2.179429404046676 120 57 -2.189742423562386 120 58 -2.1167114378166225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 559.0 3 67.0 4 186.0 5 139.0 6 228.0 7 608.0 8 520.0 9 914.0 10 974.0 11 1025.0 12 1415.0 13 1662.0 14 1857.0 15 1954.0 16 2184.0 17 2300.0 18 2717.0 19 2876.0 20 3539.0 21 2792.0 22 3533.0 23 3475.0 24 3311.0 25 3221.0 26 3508.0 27 4060.0 28 4728.0 29 6178.0 30 7460.0 31 9224.0 32 11314.0 33 14167.0 34 17542.0 35 22937.0 36 31132.0 37 49765.0 38 71476.0 39 76614.0 40 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 41.447550400545694 40.37334135409421 6.237126275958933 11.941981969401155 11 28.63169937185626 33.36144180338215 15.059336622203364 22.94752220255823 12 45.280865382807825 13.416870990179044 23.11993275451369 18.182330872499442 13 21.516138648161625 14.452408510958275 29.05064089997234 34.980811940907756 14 19.829630445232795 28.873396407307844 32.78539720222254 18.51157594523682 15 34.08894988009141 16.242846439131288 31.71065105124783 17.95755262952947 16 24.951391649031592 17.97998732423113 36.23282594450591 20.835795082231364 17 35.810466046846386 18.130280423024658 21.530698291465924 24.528555238663035 18 35.38954848308255 19.266476352630605 23.872416191552954 21.471558972733888 19 22.704848252099442 23.678903000045654 33.10443360072618 20.51181514712873 20 25.377655674704524 32.46858576174325 22.7256736034461 19.428084960106133 21 24.138319985819944 22.514327763364967 33.16360237838186 20.18374987243322 22 26.406546247509173 21.659603723218552 23.82252944683457 28.11132058243771 23 29.274181419731864 30.313251976624265 23.700154692334134 16.71241191130973 24 27.84886952925474 31.178917573254346 22.646743669551814 18.325469227939102 25 24.335197091034093 21.06046201855164 23.152221201720888 31.45211968869338 26 31.114811780663704 18.146143618807162 27.818589597238823 22.920455003290314 27 18.703420715511523 19.33240051671953 26.81731386414359 35.146864903625364 28 20.37176318899753 31.62726979692704 28.208069195229395 19.79289781884603 29 32.390655049045414 19.287256787391087 28.5862780278694 19.735810135694106 30 20.383883760169372 26.76278090038582 33.40095003707724 19.45238530236757 31 32.10351831155434 17.24391764099037 33.260079951856945 17.39248409559835 32 27.994499296296695 15.119414690746568 42.467957326572055 14.418128686384685 33 31.280869544198335 14.265993076090489 35.8840070581979 18.56913032151327 34 24.04024613963133 17.456199017482792 41.82574152115581 16.677813321730067 35 18.386318707292965 30.68540762387726 40.794888048476516 10.133385620353259 36 22.753844335314156 26.9879861163348 35.849031259738446 14.4091382886126 37 22.038515684609848 23.54975121704835 41.864045221540415 12.547687876801392 38 22.662105401766638 24.295185142387247 39.73003259709931 13.3126768587468 39 22.377485222998388 21.26518001074691 39.82240730789898 16.53492745835572 40 20.03353207176078 22.147825923408234 42.33665877624059 15.48198322859039 41 18.01215534335352 24.066720045569102 41.643067101579334 16.27805750949804 42 17.71730045125068 21.040537308137832 40.07799460864284 21.164167631968652 43 17.730058052031822 21.78778757256504 40.77160825628897 19.71054611911417 44 17.8784174111641 20.059649358500707 41.62826627258682 20.43366695774837 45 19.99156349411057 19.777158025965093 43.27202089244261 16.95925758748173 46 18.885111985919465 23.78669604589609 41.49081675153502 15.837375216649423 47 19.44100197049897 20.370335522477937 44.139176906719854 16.049485600303235 48 22.187399204386626 18.363885603698527 43.09697881948178 16.35173637243307 49 21.847155678157954 19.058488871681533 42.43414154124119 16.660213908919324 50 15.342154155564458 20.10147261664377 44.7974446323567 19.758928595435076 51 14.267089944814352 20.298803549300164 46.01993994898083 19.414166556904657 52 15.099471599124886 19.641407707234738 47.31875527460182 17.940365419038557 53 13.15086908010593 20.04758768097434 47.997150116280636 18.804393122639098 54 13.252331774783762 22.258937909858656 46.11308133047614 18.375648984881444 55 15.77633737661709 19.95185713133052 42.80573411150856 21.466071380543827 56 15.060670373329243 22.24562422833902 40.13982026183348 22.553885136498252 57 18.49181139170031 22.763497284808647 39.51591863301748 19.228772690473566 58 18.614128241958653 26.065589645099337 38.85481727753479 16.465464835407218 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 150.0 7 300.0 8 413.5 9 595.0 10 663.0 11 948.0 12 1233.0 13 2560.5 14 3888.0 15 4549.0 16 5704.5 17 6199.0 18 6828.5 19 7458.0 20 8787.5 21 10117.0 22 11474.0 23 13803.0 24 14775.0 25 15104.0 26 15433.0 27 14433.5 28 11913.0 29 10392.0 30 14985.0 31 19578.0 32 13963.5 33 8349.0 34 7150.0 35 5630.0 36 5309.0 37 5596.0 38 5883.0 39 6252.0 40 6621.0 41 7475.5 42 9146.5 43 9963.0 44 9374.5 45 8786.0 46 8874.0 47 9732.5 48 10503.0 49 13838.0 50 17173.0 51 16864.0 52 16555.0 53 16204.0 54 18728.5 55 21604.0 56 23010.5 57 24417.0 58 24057.5 59 25219.0 60 26740.0 61 19092.0 62 11444.0 63 8329.0 64 5214.0 65 4265.0 66 2720.5 67 2125.0 68 1894.0 69 1663.0 70 1263.5 71 864.0 72 597.5 73 271.5 74 212.0 75 170.0 76 128.0 77 115.5 78 119.0 79 135.0 80 96.5 81 58.0 82 41.5 83 25.0 84 16.5 85 6.5 86 5.0 87 3.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 8.056567848386135E-4 17 2.6855226161287115E-4 18 0.0 19 0.001611313569677227 20 0.0010742090464514846 21 0.0034911794009673254 22 2.6855226161287115E-4 23 0.004028283924193067 24 0.0 25 2.6855226161287115E-4 26 0.016650240219998013 27 0.004833940709031682 28 0.02336404676031979 29 0.0158445834351594 30 0.04592243673580097 31 0.03840297341064058 32 0.01369616534225643 33 0.00913077689483762 34 0.015576031173546528 35 0.016918792481610885 36 0.03518034627128612 37 0.041625600549995034 38 0.06660096087999205 39 0.044848227689349485 40 0.049950720659994036 41 0.04968216839838117 42 0.07788015586773264 43 0.07707449908289403 44 0.05156203422967126 45 0.046996645782252454 46 0.05961860207805741 47 0.10204985941289105 48 0.08781658954740887 49 0.11628312927837321 50 0.11923720415611481 51 0.09399329156450491 52 0.08083423074547422 53 0.11332905440063162 54 0.11816299510966331 55 0.14958360971836926 56 0.16220556601417419 57 0.15307478911933656 58 0.1613999092293356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 372367.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.073830627803765 #Duplication Level Percentage of deduplicated Percentage of total 1 71.57373524977638 22.240701265508196 2 11.026101423218318 6.852464162201442 3 5.078620913876833 4.7343661830189205 4 2.814597789875769 3.4984134003196186 5 1.8230460385150946 2.832451191375334 6 1.2365256957800523 2.3054154022557927 7 0.970661305130178 2.1113515494804673 8 0.8018086884332889 1.9932213904222003 9 0.5870688550798028 1.6418230352647618 >10 3.614195996007047 19.88618040549654 >50 0.24504491140574627 5.418391528856967 >100 0.19482369281375586 11.689981962173434 >500 0.023378843137650703 5.033900442615204 >1k 0.009524713870894732 6.737069495036726 >5k 0.0 0.0 >10k+ 8.658830791722483E-4 3.024268585974392 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 11240 3.018527420528672 No Hit ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 4534 1.2176159541527578 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 4298 1.1542376204121203 No Hit ACAATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 3840 1.0312406845934252 No Hit ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 2928 0.7863210220024869 No Hit ACAATAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 2054 0.5516063453528374 No Hit ACAATAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1434 0.38510394315285723 No Hit ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.3716763300722137 No Hit ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAGCAGTGG 1280 0.34374689486447507 No Hit ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 1179 0.31662311644157515 No Hit ACAATAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 1085 0.29137920384996524 No Hit ACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1023 0.27472896362996724 No Hit ACAATAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 997 0.2677466048280326 No Hit ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 938 0.25190202139287315 No Hit ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 923 0.2478737374686801 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA 902 0.2422341399748098 No Hit ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 871 0.23390901986481077 No Hit ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 858 0.23041784046384348 No Hit ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 827 0.22209272035384445 No Hit ACAATAGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 824 0.22128706356900585 No Hit ACAATAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 808 0.21699022738319992 No Hit ACAATAGGGCAGGACCATGTACACATACATGTGTGCCTGTGGTTTAAAAGGCAAAAAT 796 0.21376760024384547 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGA 756 0.2030255097793306 No Hit ACAATAGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC 642 0.1724105519554633 No Hit ACAATAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 630 0.16918792481610884 No Hit ACAATAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 629 0.16891937255449596 No Hit ACAATAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 625 0.1678451635080445 No Hit ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 622 0.1670395067232059 No Hit ACAATAGGGGAGTGCAGTGCTACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGA 609 0.16354832732223856 No Hit ACAATAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 584 0.15683452078191676 No Hit ACAATAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGTTCTTCCGAA 576 0.1546861026890138 No Hit ACAATAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 555 0.1490465051951435 No Hit ACAATAGGGACCTAGAAAGGCAAGTTCTAGAGTTCTCTTTTTCCCTTATACCCAAGGC 548 0.1471666393638534 No Hit ACAATAGGGATGATCGGAAGGACGGACGGAACCGGGCTTGTGTGGAGAGAGTCTGATG 546 0.14662953484062766 No Hit ACAATAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA 542 0.14555532579417618 No Hit ACAATAGGGACGCCATGATGTCGATGCTCTCCTCTGGCAACCACACTGCAGCAAGCAG 538 0.1444811167477247 No Hit ACAATAGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCC 525 0.14098993734675738 No Hit ACAATAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 525 0.14098993734675738 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 513 0.1377673102074029 No Hit ACAATAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 481 0.12917363783579103 No Hit ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 472 0.1267566674812752 Illumina Single End Adapter 2 (96% over 25bp) ACAATAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 461 0.12380259260353361 No Hit ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 448 0.12031141320256629 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 427 0.11467181570869599 No Hit ACAATAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 419 0.11252339761579302 No Hit ACAATAGGGAAGCACAAGATGTGTTAGACTGAAAAAAGAACTGATCTCTTAGAACCGT 411 0.11037497952289005 No Hit ACAATAGGGATGCCGTTGTGAAAGTCCTACAAGTAAATCTCTATATTCATGATAATCT 401 0.10768945690676135 No Hit ACAATAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC 400 0.10742090464514846 No Hit ACAATAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG 395 0.10607814333708412 No Hit ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC 379 0.10178130715127819 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 376 0.10097565036643957 No Hit ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA 375 0.10070709810482668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.013159060819030687 0.0 0.0 0.0 0.0 11 0.03276337591677028 0.0 0.0 0.0 0.0 12 0.033569032701608895 0.0 0.0 0.0 0.0 13 0.03437468948644751 0.0 0.0 0.0 0.0 14 0.05021927292160691 0.0 0.0 0.0 0.0 15 0.051024929706445524 0.0 0.0 0.0 0.0 16 0.05236769101450988 0.0 0.0 0.0 0.0 17 0.05263624327612275 0.0 0.0 0.0 0.0 18 0.05344190006096136 0.0 0.0 0.0 0.0 19 0.05559031815386433 0.0 0.0 0.0 0.0 20 0.07036069254257225 0.0 0.0 0.0 0.0 21 0.07197200611224948 0.0 0.0 0.0 0.0 22 0.07465752872837819 0.0 0.0 0.0 0.0 23 0.08137133526869997 0.0 0.0 0.0 0.0 24 0.09614170965740788 0.0 0.0 0.0 0.0 25 0.09748447096547223 0.0 0.0 0.0 0.0 26 0.0988272322735366 0.0 0.0 0.0 0.0 27 0.10258696393611678 0.0 0.0 0.0 0.0 28 0.1759017313564306 0.0 0.0 0.0 0.0 29 0.1788558062341722 0.0 0.0 0.0 0.0 30 0.21484180929029695 0.0 0.0 0.0 0.0 31 0.21725877964481277 0.0 0.0 0.0 0.0 32 0.2199443022609415 0.0 0.0 0.0 0.0 33 0.2226298248770702 0.0 0.0 0.0 0.0 34 0.22477824296997317 0.0 0.0 0.0 0.0 35 0.3176973254880266 0.0 0.0 0.0 0.0 36 0.32199416167383255 0.0 0.0 0.0 0.0 37 0.32548534107479987 0.0 0.0 0.0 0.0 38 0.3305878340454444 0.0 0.0 0.0 0.0 39 0.335690327016089 0.0 0.0 0.0 0.0 40 0.34240413355641075 0.0 0.0 0.0 0.0 41 0.3475066265270553 0.0 0.0 0.0 0.0 42 0.35744306020673156 0.0 0.0 0.0 0.0 43 0.3614713441309246 0.0 0.0 0.0 0.0 44 0.36576818031673053 0.0 0.0 0.0 0.0 45 0.36925935971769785 0.0 0.0 0.0 0.0 46 0.3724819868570523 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTAC 15 0.007182732 52.061375 45 CCCTAAC 25 1.6415153E-5 52.061375 50 CCTAACG 25 1.6415153E-5 52.061375 51 CGTTACC 15 0.007182732 52.061375 46 TAGCACC 15 0.007182732 52.061375 45 GTTACAT 15 0.007182732 52.061375 52 ACTAGGT 15 0.007182732 52.061375 46 CGAATGA 15 0.007182732 52.061375 47 CGGGACA 30 7.915751E-7 52.061375 46 CGCGTAG 15 0.007182732 52.061375 46 CAATATG 15 0.007182732 52.061375 45 GCGTAGG 15 0.007182732 52.061375 47 GTCGGAC 15 0.007182732 52.061375 51 AACGTCA 20 3.4246282E-4 52.06137 49 TATTATC 20 3.4246282E-4 52.06137 44 TATAGCC 20 3.4246282E-4 52.06137 47 CCGTAGG 20 3.4269047E-4 52.05437 42 AGGTTAG 20 3.4269047E-4 52.05437 48 CCGACTA 20 3.4269047E-4 52.05437 42 CGACTAC 20 3.4269047E-4 52.05437 43 >>END_MODULE