FastQCFastQC Report
Tue 31 May 2016
SRR212586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371169
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT110382.9738474926515956No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT43261.1655068176491032No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA41931.1296740837731598No Hit
ACAATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC36100.9726027766327469No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG28310.7627253353593646No Hit
ACAATAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG19690.5304861127949801No Hit
ACAATAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC13480.36317688169001183No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12420.33461846220993674No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA11910.3208780905732968No Hit
ACAATAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT10530.2836982614388594No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAGCAGTGG10020.26995788980221946No Hit
ACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC9950.2680719564403277No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9330.25136797523500076No Hit
ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA8940.2408606322187467No Hit
ACAATAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8680.23385573687457734No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA8450.22765909868550446No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.22469548911681742No Hit
ACAATAGGGCAGGACCATGTACACATACATGTGTGCCTGTGGTTTAAAAGGCAAAAAT8230.22173187954813034No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.22065420334133506No Hit
ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.21930710808284096No Hit
ACAATAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7830.21095511748017748No Hit
ACAATAGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT7470.2012560316190199No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGA7460.2009866125673211No Hit
ACAATAGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC6420.17296703119064363No Hit
ACAATAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6380.17188935498384833No Hit
ACAATAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC6300.16973400257025775No Hit
ACAATAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5970.16084317386419664No Hit
ACAATAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5890.15868782145060606No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC5700.15356885946832846No Hit
ACAATAGGGACCTAGAAAGGCAAGTTCTAGAGTTCTCTTTTTCCCTTATACCCAAGGC5470.14737222127925556No Hit
ACAATAGGGGAGTGCAGTGCTACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGA5400.1454862879173638No Hit
ACAATAGGGACGCCATGATGTCGATGCTCTCCTCTGGCAACCACACTGCAGCAAGCAG5370.14467803076226732No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGTTCTTCCGAA5290.14252267834867677No Hit
ACAATAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5260.1417144211935803No Hit
ACAATAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5250.14144500214188147No Hit
ACAATAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA5200.14009790688338736No Hit
ACAATAGGGATGATCGGAAGGACGGACGGAACCGGGCTTGTGTGGAGAGAGTCTGATG5150.13875081162489322No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC5130.1382119735214956Illumina Single End Adapter 2 (96% over 25bp)
ACAATAGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCC4840.13039882102222977No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4760.1282434686086392No Hit
ACAATAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC4600.12393276378145804No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4400.11854438274748161No Hit
ACAATAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4340.11692786843728867No Hit
ACAATAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG4300.11585019223049339No Hit
ACAATAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4100.11046181119651695No Hit
ACAATAGGGATGCCGTTGTGAAAGTCCTACAAGTAAATCTCTATATTCATGATAATCT3930.10588168731763697No Hit
ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC3890.10480401111084169No Hit
ACAATAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC3790.10210982059385347No Hit
ACAATAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3750.10103214438705818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT401.8499122E-952.12329524
TCGTTTA203.4045737E-452.12329527
ACCGGCT203.4045737E-452.12329525
CGCGATC401.8499122E-952.12329525
GCGCTAG203.4045737E-452.12329526
AAACGAC150.007149028652.1232924
ACGGAAC503.6379788E-1252.1232926
CAAGCGT307.850995E-752.1232924
ACACTCA251.6299944E-552.1232926
TGCACTC150.007149028652.1232926
GTTAGAC353.8184226E-852.09512323
CGATTGG203.4136735E-452.09512323
AGCGAGA203.4136735E-452.09512323
CAGCGAG203.4159518E-452.08808522
TACGGAC150.00716815952.08808522
TCGATCA307.887702E-752.08808521
AACGCGT150.00716815952.08808522
CGATCAT307.887702E-752.08808522
CTAGGCA150.00716815952.08808522
CTTACTT251.6365278E-552.0880821
Produced by FastQC (version 0.11.5)