##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212586_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 371169 Sequences flagged as poor quality 0 Sequence length 58 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.84863498837457 39.0 38.0 40.0 34.0 40.0 2 37.71901209422123 39.0 38.0 40.0 33.0 40.0 3 37.469955734449805 39.0 38.0 40.0 33.0 40.0 4 37.745296616904966 39.0 38.0 40.0 34.0 40.0 5 37.547720849532155 39.0 38.0 40.0 33.0 40.0 6 37.68274559567205 39.0 38.0 40.0 33.0 40.0 7 37.615323477984425 39.0 38.0 40.0 34.0 40.0 8 37.407121823212606 39.0 38.0 40.0 33.0 40.0 9 37.305243703003214 39.0 38.0 40.0 33.0 40.0 10 36.80403535855635 39.0 36.0 40.0 31.0 40.0 11 37.28522586746199 39.0 37.0 40.0 33.0 40.0 12 37.13210424361948 39.0 36.0 40.0 32.0 40.0 13 37.06050343644 39.0 36.0 40.0 32.0 40.0 14 37.05325067556827 39.0 36.0 40.0 33.0 40.0 15 37.02018218116276 39.0 36.0 40.0 33.0 40.0 16 36.78038306000771 39.0 36.0 40.0 32.0 40.0 17 36.63075580126573 38.0 36.0 40.0 31.0 40.0 18 36.4834347696063 38.0 36.0 40.0 31.0 40.0 19 36.23637211081744 38.0 35.0 40.0 30.0 40.0 20 36.339220139612955 38.0 35.0 40.0 31.0 40.0 21 36.293847277116356 38.0 36.0 40.0 31.0 40.0 22 36.19632566297293 38.0 35.0 40.0 30.0 40.0 23 35.95503126608095 38.0 35.0 40.0 30.0 40.0 24 35.81965627517384 38.0 35.0 40.0 29.0 40.0 25 35.84854608008751 38.0 35.0 40.0 30.0 40.0 26 35.45850542475261 38.0 35.0 39.0 29.0 40.0 27 35.04295078522183 38.0 35.0 39.0 27.0 40.0 28 34.867575147709 38.0 34.0 39.0 27.0 40.0 29 34.984026144424774 38.0 35.0 39.0 27.0 40.0 30 34.52942190754077 38.0 34.0 39.0 26.0 40.0 31 34.041778812346934 38.0 33.0 39.0 24.0 40.0 32 33.621466232363154 38.0 33.0 39.0 22.0 40.0 33 33.644097432705856 38.0 33.0 39.0 23.0 40.0 34 33.209734649175985 38.0 33.0 39.0 20.0 40.0 35 32.85223712109578 37.0 32.0 39.0 18.0 40.0 36 33.32096969305088 38.0 33.0 39.0 16.0 40.0 37 33.215088005733236 38.0 33.0 39.0 17.0 40.0 38 33.05521743464568 38.0 33.0 39.0 15.0 40.0 39 33.03342951593479 38.0 33.0 39.0 15.0 40.0 40 32.88625127637276 38.0 33.0 39.0 14.0 40.0 41 33.12540918018477 38.0 33.0 39.0 9.0 40.0 42 32.987296891712404 38.0 33.0 39.0 8.0 40.0 43 32.80910851929983 38.0 33.0 39.0 6.0 40.0 44 32.74373398640511 38.0 33.0 39.0 2.0 40.0 45 32.547292473239956 38.0 33.0 39.0 2.0 40.0 46 32.07631025220317 38.0 32.0 39.0 2.0 40.0 47 31.901112431264465 38.0 32.0 39.0 2.0 40.0 48 31.878831475688973 38.0 32.0 39.0 2.0 40.0 49 31.692673687727154 37.0 32.0 39.0 2.0 40.0 50 31.459774388486107 37.0 31.0 39.0 2.0 40.0 51 31.196207118590184 37.0 31.0 39.0 2.0 40.0 52 31.028916746818833 37.0 31.0 39.0 2.0 40.0 53 30.858288272996937 37.0 31.0 39.0 2.0 40.0 54 30.613655774054408 36.0 30.0 39.0 2.0 40.0 55 30.360345287456656 36.0 30.0 39.0 2.0 40.0 56 30.03400876689594 37.0 30.0 39.0 2.0 40.0 57 29.69593365825271 36.0 29.0 39.0 2.0 40.0 58 29.33528662145815 36.0 28.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5566038837169742 1 2 -1.048142999226556 1 3 -1.189119970097856 1 4 -1.2183649224127606 1 5 -1.5628820833132835 1 6 -1.3680128489446304 1 7 -1.6166958797513047 1 8 -1.588948055447851 1 9 -1.956553101281024 1 10 -1.5526457316467699 1 11 -1.355795481724698 1 12 -1.730733473153542 1 13 -1.5593886898468199 1 14 -1.8718502731371345 1 15 -2.2318605204355393 1 16 -1.3937185629421194 1 17 -1.5609769044966555 1 18 -1.3585897992964178 1 19 -2.0268987961267015 1 20 -1.5661882203680761 1 21 -1.4708700947107047 1 22 -1.1061418884787102 1 23 -1.496754353883901 1 24 -1.5896198689982697 1 25 -1.185156770126163 1 26 -1.2637421881080684 1 27 -0.7132898273474737 1 28 -1.3288154273941615 1 29 -1.8476556843422216 1 30 -1.4772153240847956 1 31 -1.5933782175750082 1 32 -2.0418981187046086 1 33 -1.5345110151193069 1 34 -2.087230533009066 1 35 -1.775696885068502 1 36 -2.1529059770376158 1 37 -2.088648751120342 1 38 -2.14276917020441 1 39 -1.9023483435353086 1 40 -1.8276146140002396 1 41 -1.7600325661726401 1 42 -1.8817014425628429 1 43 -1.8776048651704684 1 44 -2.264632187730925 1 45 -2.183511314429431 1 46 -1.9856681196727521 1 47 -1.764399314928479 1 48 -1.9772202813093998 1 49 -2.175532021250678 1 50 -2.056610695557204 1 51 -2.0545185318756616 1 52 -2.140418139507304 1 53 -1.5931493430581725 1 54 -1.7491325323739169 1 55 -1.278864928458539 1 56 -1.4919569003724007 1 57 -1.5603659976453699 1 58 -1.605165177659572 2 1 -0.6004703433443055 2 2 -0.812376953677898 2 3 -1.1277845663711474 2 4 -1.1580647153734205 2 5 -1.0279441951145216 2 6 -0.9690739255491891 2 7 -1.1779018838920905 2 8 -1.9048124446818022 2 9 -1.9908439915501717 2 10 -0.8710981125991495 2 11 -0.4752312995300798 2 12 -0.638342168805714 2 13 -0.4343886898468199 2 14 -0.6393192172365119 2 15 -0.527538574265769 2 16 -0.1494121447226604 2 17 -0.624512101184024 2 18 -0.7557171284889606 2 19 -0.6270540756298075 2 20 -0.8599252804094846 2 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117 57 1.0363277433961962 117 58 1.1029189121636485 118 1 -0.4391383577198624 118 2 -0.6557057946482487 118 3 -0.45561475505039084 118 4 -0.6107825949780974 118 5 -0.4808866928683386 118 6 -0.6780249587926761 118 7 -0.9306534562191331 118 8 -0.9428290664248422 118 9 -1.2314661838233079 118 10 -0.25974366515979597 118 11 -0.6112937433755405 118 12 -0.3983376764427362 118 13 -0.48077233764556127 118 14 -0.3661273933371376 118 15 -0.2090861933133823 118 16 -0.1965819560434099 118 17 -0.4245457939063968 118 18 -0.21803743396964137 118 19 -0.6556928896459766 118 20 -0.1594535822962726 118 21 -0.38151113537635695 118 22 -0.33056471749547 118 23 -0.2987583384145438 118 24 -0.5232110249027926 118 25 -0.3712577896972391 118 26 -0.2892705290588893 118 27 -0.14916622876940266 118 28 -0.5829855121630985 118 29 -0.27345356707201063 118 30 -0.3792232150180297 118 31 -0.7258052655065654 118 32 -0.6698132716402725 118 33 -0.5262294416203446 118 34 -0.6951839686901451 118 35 -0.41292880819050026 118 36 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-0.3140613176914968 119 16 -0.40712358750872824 119 17 -0.5856084053773429 119 18 -0.5384729920284101 119 19 -0.631051943433647 119 20 -0.6374128425985361 119 21 -0.8592357200004059 119 22 -0.5899001159747712 119 23 -0.52773609116025 119 24 -0.9556901387674301 119 25 -0.9080361619867361 119 26 -0.7200599780403962 119 27 -0.30887710833384574 119 28 -0.5021444345323829 119 29 -0.8474702759802994 119 30 -0.52355814414598 119 31 -0.5072189517921188 119 32 -0.6196583856926523 119 33 -0.43319461574969864 119 34 -0.7534398590496707 119 35 -0.2388933252279557 119 36 -0.1435243139246083 119 37 -0.4653985425359366 119 38 -0.3048717010285529 119 39 -0.1421462484208149 119 40 -0.3491868769093003 119 41 -0.25412730984557896 119 42 -0.12650537322011246 119 43 -0.5868196607577474 119 44 -0.2195408741126812 119 45 -0.1298912161629957 119 46 0.05527931705664457 119 47 -0.1547488096906946 119 48 0.0264229930022708 119 49 0.38968537005366954 119 50 0.40798557773472055 119 51 0.7464177803520187 119 52 0.6681384563951696 119 53 0.31139316180879817 119 54 1.0546590686272879 119 55 0.8497343113671789 119 56 0.8294206837566449 119 57 0.5491176400666902 119 58 0.3825515183634174 120 1 -1.2239267598091956 120 2 -2.014697373371199 120 3 -1.970581176540641 120 4 -1.815227750078833 120 5 -2.3272783355503606 120 6 -2.1118806244999533 120 7 -2.9248651607282454 120 8 -3.614729716675349 120 9 -3.6594376154404102 120 10 -2.821410864818681 120 11 -1.2360565618383887 120 12 -1.3900976167476031 120 13 -1.5034564864569901 120 14 -1.2022429460500703 120 15 -1.4593686791890903 120 16 -1.0025194893554286 120 17 -1.2911485015068678 120 18 -0.9460621648085734 120 19 -1.158954802022059 120 20 -1.4693885572456438 120 21 -1.4706700736558602 120 22 -0.8780863036803126 120 23 -1.292522329460276 120 24 -1.1131907711984965 120 25 -0.9409731718526615 120 26 -0.826611963873944 120 27 -0.9797599821006102 120 28 -1.743480128941691 120 29 -1.7729951937642667 120 30 -1.5989439270956325 120 31 -2.2789872290499 120 32 -2.2192536298110426 120 33 -1.6861164472700594 120 34 -2.044762904837679 120 35 -1.631860691784997 120 36 -1.600847865657471 120 37 -2.0801215377294575 120 38 -1.456402184205885 120 39 -1.5200870528731336 120 40 -1.422498285965709 120 41 -1.3349772971969607 120 42 -1.1017140754715697 120 43 -1.6841634502846894 120 44 -1.2366924045958605 120 45 -1.13135845623383 120 46 -0.8853733518024498 120 47 -1.01323603458318 120 48 -1.067429777045792 120 49 -0.8950603926581948 120 50 -0.9716754392144331 120 51 -1.0598559358129194 120 52 -1.0148364993346561 120 53 -0.8368486798304637 120 54 -1.2908000763259118 120 55 -1.3031622229105864 120 56 -1.5388565740222582 120 57 -1.425888684243958 120 58 -1.3717616614999777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 481.0 3 66.0 4 173.0 5 151.0 6 236.0 7 652.0 8 557.0 9 891.0 10 1074.0 11 1028.0 12 1387.0 13 1703.0 14 1860.0 15 1969.0 16 2293.0 17 2345.0 18 2746.0 19 3003.0 20 3358.0 21 2708.0 22 3573.0 23 3429.0 24 3434.0 25 3316.0 26 3723.0 27 4189.0 28 5204.0 29 6473.0 30 7858.0 31 9448.0 32 11641.0 33 14880.0 34 18427.0 35 23951.0 36 32120.0 37 50615.0 38 70012.0 39 70001.0 40 194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 41.56085233411196 40.165261646312054 6.210378560709541 12.063507458866447 11 28.581589518521213 33.25654890359917 15.014184913071944 23.147676664807676 12 45.101018673434474 13.489273080456613 23.303670295741295 18.10603795036762 13 21.505567544703354 14.43197034235079 29.300130129401968 34.76233198354389 14 19.960449283210615 28.668342453168233 32.861580573808695 18.509627689812458 15 33.902076951469546 16.24677707459405 31.816773491320667 18.034372482615733 16 25.063852487283388 18.09638761962238 35.990171566514356 20.849588326579877 17 35.59806988191363 18.247213533457803 21.555410069267637 24.599306515360926 18 35.094333521075136 19.343963651748826 24.008494193927408 21.553208633248623 19 22.93750757749249 23.63881292686541 33.00068702598575 20.422992469656354 20 25.326267350633675 32.39934477110096 22.63206957496336 19.64231830330201 21 24.096301145839227 22.60506669468625 32.94240628931834 20.356225870156184 22 26.540613793790936 21.776451031476398 23.70523241559729 27.977702759135376 23 29.11161522545047 30.158311061298388 23.83368178291232 16.896391930338822 24 27.908204919556706 30.903104294709955 22.758540784790277 18.430150000943062 25 24.353385565410438 21.217305557651063 23.097606448901296 31.3317024280372 26 30.918937852802884 18.19822975467148 27.87799587751088 23.004836515014752 27 18.889345303404554 19.6373846727598 26.6664684866796 34.80680153715605 28 20.456241798452787 31.283496668184597 28.293889561030287 19.966371972332325 29 32.08586411136681 19.2827770561954 28.745237107179232 19.88612172525856 30 20.402936426844096 26.581780242473585 33.52115106206882 19.4941322686135 31 31.695152281049676 17.425489662456922 33.2940830937452 17.585274962748198 32 27.821001711290478 15.264172045328985 42.342985730262896 14.571840513117648 33 30.951245084483542 14.438074599953103 35.85180273786518 18.758877577698176 34 24.037181373738026 17.571675749098496 42.00638195803216 16.38476091913132 35 18.494221398076675 30.281868558368192 40.98953167450084 10.234378369054294 36 22.41972832577185 27.008914731816724 35.90855054062578 14.662806401785641 37 21.94738601561489 23.53152094206962 41.765787430444725 12.755305611870767 38 22.2834218976213 24.29195749992586 39.971206496332066 13.453414106120775 39 22.363673625680217 21.303102702527788 40.01504700165571 16.318176670136285 40 19.977569650011056 22.155092929587035 42.188199263464945 15.679138156936965 41 17.99679119871648 23.932534278896064 41.632443947095226 16.43823057529223 42 17.684919539238102 21.200493981621296 40.28296086975279 20.831625609387807 43 17.824846723357904 21.645573715684634 40.904777054608005 19.624802506349454 44 17.90333670374115 19.843882709807886 41.71782945736434 20.534951129086622 45 20.137342342099444 19.790885903941245 43.10160745002669 16.970164303932616 46 18.93268546282115 23.59568134262355 41.62424229350476 15.847390901050543 47 19.185652904510032 20.51403772343163 44.03710306917581 16.263206302882526 48 21.85304358849184 18.617553531501255 43.06012196470522 16.46928091530169 49 21.559498150840685 19.20050497829834 42.46646291295868 16.77353395790229 50 15.277931397278014 20.10116406113007 44.84265616358903 19.778248378002885 51 14.348606034447876 20.32613533672327 46.204933842273505 19.120324786555347 52 14.94043300364927 19.868162337259513 47.24416669588601 17.94723796320521 53 13.128019225945318 20.35635969348535 48.020089062950944 18.495532017618387 54 13.444924697413649 22.122394357837027 46.12020354092133 18.312477403827994 55 15.513765454255942 20.171970345101194 42.871947944695286 21.442316255947578 56 14.97423077026914 22.336752132137157 40.0701398206818 22.618877276911903 57 18.287537555776908 22.799359695936552 39.65836053005947 19.25474221822707 58 18.444080954000462 26.211259926258222 38.94332248260374 16.401336637137568 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 154.5 7 309.0 8 422.5 9 611.0 10 686.0 11 938.0 12 1190.0 13 2468.0 14 3746.0 15 4428.0 16 5510.5 17 5911.0 18 6741.0 19 7571.0 20 8904.0 21 10237.0 22 11427.0 23 13809.0 24 15001.0 25 15277.5 26 15554.0 27 14567.5 28 12089.0 29 10597.0 30 14990.5 31 19384.0 32 13927.0 33 8470.0 34 7244.0 35 5713.0 36 5408.0 37 5568.0 38 5728.0 39 6224.0 40 6720.0 41 7657.0 42 9363.5 43 10133.0 44 9347.5 45 8562.0 46 8744.0 47 9815.5 48 10705.0 49 13640.5 50 16576.0 51 16556.0 52 16536.0 53 16336.0 54 18473.5 55 20811.0 56 22482.0 57 24153.0 58 23876.0 59 24933.5 60 26268.0 61 18777.0 62 11286.0 63 8288.5 64 5291.0 65 4384.5 66 2774.5 67 2071.0 68 1859.5 69 1648.0 70 1277.5 71 907.0 72 643.0 73 306.5 74 234.0 75 186.5 76 139.0 77 124.0 78 130.5 79 152.0 80 105.0 81 58.0 82 38.5 83 19.0 84 18.0 85 12.0 86 7.0 87 3.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.6941905169882184E-4 17 0.0 18 0.02478655275629161 19 0.0010776762067952873 20 2.6941905169882184E-4 21 0.0 22 5.388381033976437E-4 23 2.6941905169882184E-4 24 0.010237923964555229 25 0.0013470952584941091 26 0.009160247757759943 27 0.3057906236781628 28 0.013470952584941092 29 0.01912875267061635 30 0.06519941051111489 31 0.012393276378145805 32 0.02801958137667747 33 0.04041285775482327 34 0.03313854335895508 35 0.03906576249632917 36 0.05657800085675258 37 0.06492999145941605 38 0.0678936010281031 39 0.08917770611231002 40 0.0654688295628137 41 0.08459758223343006 42 0.08217281076814066 43 0.07301256301038071 44 0.07920920119945363 45 0.07301256301038071 46 0.08432816318173122 47 0.11261716361010753 48 0.10938413498972166 49 0.12393276378145804 50 0.13039882102222977 51 0.1250104399882533 52 0.11046181119651695 53 0.11315600171350516 54 0.14306151645207438 55 0.17242819308724597 56 0.20637499360129752 57 0.19398171722315172 58 0.1850908885170906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 371169.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.196326807269806 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2696929684886 23.268186530745076 2 10.870613176086326 6.9998762882537715 3 5.0141198280607355 4.843087219051647 4 2.8143991353251 3.624532573080979 5 1.7409098768240188 2.8025451668114965 6 1.250164985095334 2.4150432253880973 7 0.823854652693601 1.8567565547869056 8 0.7431389247883755 1.9141074948551706 9 0.6103134238746626 1.7684865404939176 >10 3.4105226059698914 19.594889389795767 >50 0.23033846811408437 5.254300329929594 >100 0.1899872036269455 11.49677613529178 >500 0.02269758627401561 5.012331314581267 >1k 0.008406513434820597 6.161548808058888 >5k 0.0 0.0 >10k+ 8.406513434820596E-4 2.987532428875643 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 11038 2.9738474926515956 No Hit ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 4326 1.1655068176491032 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 4193 1.1296740837731598 No Hit ACAATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 3610 0.9726027766327469 No Hit ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 2831 0.7627253353593646 No Hit ACAATAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1969 0.5304861127949801 No Hit ACAATAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1348 0.36317688169001183 No Hit ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1242 0.33461846220993674 No Hit ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 1191 0.3208780905732968 No Hit ACAATAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 1053 0.2836982614388594 No Hit ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAGCAGTGG 1002 0.26995788980221946 No Hit ACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 995 0.2680719564403277 No Hit ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 933 0.25136797523500076 No Hit ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 894 0.2408606322187467 No Hit ACAATAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 868 0.23385573687457734 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA 845 0.22765909868550446 No Hit ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.22469548911681742 No Hit ACAATAGGGCAGGACCATGTACACATACATGTGTGCCTGTGGTTTAAAAGGCAAAAAT 823 0.22173187954813034 No Hit ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.22065420334133506 No Hit ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 814 0.21930710808284096 No Hit ACAATAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 783 0.21095511748017748 No Hit ACAATAGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 747 0.2012560316190199 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGA 746 0.2009866125673211 No Hit ACAATAGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC 642 0.17296703119064363 No Hit ACAATAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 638 0.17188935498384833 No Hit ACAATAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 630 0.16973400257025775 No Hit ACAATAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 597 0.16084317386419664 No Hit ACAATAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 589 0.15868782145060606 No Hit ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 570 0.15356885946832846 No Hit ACAATAGGGACCTAGAAAGGCAAGTTCTAGAGTTCTCTTTTTCCCTTATACCCAAGGC 547 0.14737222127925556 No Hit ACAATAGGGGAGTGCAGTGCTACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGA 540 0.1454862879173638 No Hit ACAATAGGGACGCCATGATGTCGATGCTCTCCTCTGGCAACCACACTGCAGCAAGCAG 537 0.14467803076226732 No Hit ACAATAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGTTCTTCCGAA 529 0.14252267834867677 No Hit ACAATAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 526 0.1417144211935803 No Hit ACAATAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 525 0.14144500214188147 No Hit ACAATAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA 520 0.14009790688338736 No Hit ACAATAGGGATGATCGGAAGGACGGACGGAACCGGGCTTGTGTGGAGAGAGTCTGATG 515 0.13875081162489322 No Hit ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 513 0.1382119735214956 Illumina Single End Adapter 2 (96% over 25bp) ACAATAGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCC 484 0.13039882102222977 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 476 0.1282434686086392 No Hit ACAATAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 460 0.12393276378145804 No Hit ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 440 0.11854438274748161 No Hit ACAATAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 434 0.11692786843728867 No Hit ACAATAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 430 0.11585019223049339 No Hit ACAATAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 410 0.11046181119651695 No Hit ACAATAGGGATGCCGTTGTGAAAGTCCTACAAGTAAATCTCTATATTCATGATAATCT 393 0.10588168731763697 No Hit ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC 389 0.10480401111084169 No Hit ACAATAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC 379 0.10210982059385347 No Hit ACAATAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG 375 0.10103214438705818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.014548628791736379 0.0 0.0 0.0 0.0 11 0.02775016232497865 0.0 0.0 0.0 0.0 12 0.029905514738569224 0.0 0.0 0.0 0.0 13 0.030174933790268046 0.0 0.0 0.0 0.0 14 0.04526240068540207 0.0 0.0 0.0 0.0 15 0.04553181973710089 0.0 0.0 0.0 0.0 16 0.04607065784049853 0.0 0.0 0.0 0.0 17 0.046878914995594996 0.0 0.0 0.0 0.0 18 0.047687172150691466 0.0 0.0 0.0 0.0 19 0.049034267409185574 0.0 0.0 0.0 0.0 20 0.06250521999412667 0.0 0.0 0.0 0.0 21 0.06385231525262078 0.0 0.0 0.0 0.0 22 0.06600766766621134 0.0 0.0 0.0 0.0 23 0.07705384878586305 0.0 0.0 0.0 0.0 24 0.09079422042250296 0.0 0.0 0.0 0.0 25 0.09187189662929825 0.0 0.0 0.0 0.0 26 0.09429666809458764 0.0 0.0 0.0 0.0 27 0.09833795387006997 0.0 0.0 0.0 0.0 28 0.1740447073974389 0.0 0.0 0.0 0.0 29 0.1791636693797165 0.0 0.0 0.0 0.0 30 0.21418814610056336 0.0 0.0 0.0 0.0 31 0.2174211747209492 0.0 0.0 0.0 0.0 32 0.2195765271345398 0.0 0.0 0.0 0.0 33 0.22173187954813037 0.0 0.0 0.0 0.0 34 0.22550374627191389 0.0 0.0 0.0 0.0 35 0.33219369074464733 0.0 0.0 0.0 0.0 36 0.3394680051405155 0.0 0.0 0.0 0.0 37 0.3418927766058049 0.0 0.0 0.0 0.0 38 0.34755057669148015 0.0 0.0 0.0 0.0 39 0.3521307005703601 0.0 0.0 0.0 0.0 40 0.35698024350093893 0.0 0.0 0.0 0.0 41 0.36263804358661417 0.0 0.0 0.0 0.0 42 0.3669487484137953 0.0 0.0 0.0 0.0 43 0.3715288722926753 0.0 0.0 0.0 0.0 44 0.3739536437579647 0.0 0.0 0.0 0.0 45 0.3798808628953388 0.0 0.0 0.0 0.0 46 0.38419156772251994 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGAT 40 1.8499122E-9 52.123295 24 TCGTTTA 20 3.4045737E-4 52.123295 27 ACCGGCT 20 3.4045737E-4 52.123295 25 CGCGATC 40 1.8499122E-9 52.123295 25 GCGCTAG 20 3.4045737E-4 52.123295 26 AAACGAC 15 0.0071490286 52.12329 24 ACGGAAC 50 3.6379788E-12 52.12329 26 CAAGCGT 30 7.850995E-7 52.12329 24 ACACTCA 25 1.6299944E-5 52.12329 26 TGCACTC 15 0.0071490286 52.12329 26 GTTAGAC 35 3.8184226E-8 52.095123 23 CGATTGG 20 3.4136735E-4 52.095123 23 AGCGAGA 20 3.4136735E-4 52.095123 23 CAGCGAG 20 3.4159518E-4 52.088085 22 TACGGAC 15 0.007168159 52.088085 22 TCGATCA 30 7.887702E-7 52.088085 21 AACGCGT 15 0.007168159 52.088085 22 CGATCAT 30 7.887702E-7 52.088085 22 CTAGGCA 15 0.007168159 52.088085 22 CTTACTT 25 1.6365278E-5 52.08808 21 >>END_MODULE