FastQCFastQC Report
Tue 31 May 2016
SRR212583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302079
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT92783.0713819894795735No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC38901.2877426103767557No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC33831.1199057200268803No Hit
ACAATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC28700.9500825942882491No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC25610.8477914717673192No Hit
ACAATAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC17580.581966968905485No Hit
ACAATAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA15400.5098004164473532No Hit
ACAATAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA11110.36778458615130477No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.35752236997606585No Hit
ACAATAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA8700.2880041313696086No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8110.26847281671350876No Hit
ACAATAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT7640.2529139728349207No Hit
ACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7400.244969031279897No Hit
ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC7330.24265175665968172No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT7210.2386792858821699No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.22841706970693096No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.22378252046650046No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAGCAGT6750.22345148123504116No Hit
ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.22014108892044795No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC6450.21352030429126156No Hit
ACAATAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT6180.20458224504185993No Hit
ACAATAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT6080.20127185272726672No Hit
ACAATAGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC6040.19994769580142943No Hit
ACAATAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG6010.19895457810705147No Hit
ACAATAGGGCAGGACCATGTACACATACATGTGTGCCTGTGGTTTAAAAGGCAAAA5380.1780991065251143No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCC5280.17478871421052108No Hit
ACAATAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4810.15922987033193303No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG4790.1585677918690144No Hit
ACAATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC4670.15459532109150256No Hit
ACAATAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4650.1539332426285839No Hit
ACAATAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4640.1536022033971246No Hit
ACAATAGGGACCTAGAAAGGCAAGTTCTAGAGTTCTCTTTTTCCCTTATACCCAAG4550.15062285031399072No Hit
ACAATAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA4500.1489676541566941No Hit
ACAATAGGGATGCCGTTGTGAAAGTCCTACAAGTAAATCTCTATATTCATGATAAT4380.14499518337918227No Hit
ACAATAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4310.14267790875896702No Hit
ACAATAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC4300.14234686952750772No Hit
ACAATAGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT4130.13671920259269926No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGTTCTTCCG4130.13671920259269926No Hit
ACAATAGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4070.13473296720394334No Hit
ACAATAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC4030.13340881027810605No Hit
ACAATAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3970.13142257488935014No Hit
ACAATAGGGGAGTGCAGTGCTACAATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTA3960.1310915356578908No Hit
ACAATAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG3840.12711906488037897No Hit
ACAATAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3820.12645698641746034No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG3670.12149139794557053Illumina Single End Adapter 2 (95% over 23bp)
ACAATAGGGATGATCGGAAGGACGGACGGAACCGGGCTTGTGTGGAGAGAGTCTGA3610.1195051625568146No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3520.11652580947368073No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3400.11255333869616888No Hit
ACAATAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3360.11122918177033159No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.10692567176136043No Hit
ACAATAGGGACGCCATGATGTCGATGCTCTCCTCTGGCAACCACACTGCAGCAAGC3030.10030488713217403No Hit
ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG3030.10030488713217403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATG150.00826280950.24645649
ACGCACC150.00826280950.24645650
GATTAAG301.0104177E-650.24645650
AATAAAC204.0787493E-450.24645649
GCTTAAT150.00827917450.22131348
AAATTCG600.050.22131348
ATATAGC204.092203E-450.2129446
ATGACAG150.00828463350.2129446
TATAGCC204.092203E-450.2129447
CGCGTAT204.092203E-450.2129446
ACGTTGT204.095572E-450.20456744
ATCCGAC204.1023165E-450.18783642
CACGTTG204.1023165E-450.18783643
CATCCGA204.1023165E-450.18783641
TACACGT204.1023165E-450.18783641
GCCTTAT150.00830102950.1878341
TCCCCAT150.00830102950.1878341
TAGGATG150.00830102950.1878341
CTCCAAT301.0221065E-650.1627539
CGCCTTA150.00831744950.1627540
Produced by FastQC (version 0.11.5)