FastQCFastQC Report
Tue 31 May 2016
SRR212582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169563
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT27401.6159185671402372No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13110.7731639567594346No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11240.6628804633086228No Hit
TATGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10400.6133413539510388No Hit
TATGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8100.4776985545195591No Hit
TATGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7630.44998024333138714No Hit
TATGTAGGGATGGAAATAGTCTGTGATGTTCTTGTCAACATTCAGTATTTGATTACTT6910.4075181495963152No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6730.3969026261625473No Hit
TATGTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC5320.3137476925980314No Hit
TATGTAGGGGGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAGGGTCCCTCTT4560.2689265936554555No Hit
TATGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA4320.25477256241043156No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4240.25005455199542354No Hit
TATGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA4030.23766977465602754No Hit
TATGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3340.19697693482658366No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.19166917310969964No Hit
TATGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3240.19107942180782364No Hit
TATGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT3040.17928439577030367No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3030.17869464446842764No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC2840.16748936973278367No Hit
TATGTAGGGTGAGCTTGGACAGGAGATGCCGCATCCTGCAGGGAGCCTGGAGAAGTGC2780.1639508619215277No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG2750.1621816080158997No Hit
TATGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2720.1604123541102717No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG2390.14095056114836374No Hit
TATGTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2280.13446329682772776No Hit
TATGTAGGGGATAACTGTGGATGATAACTAATTCTAATTGTTGAAGGGGGAAAAAAAC2230.13151454031834775No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.12915553511084374No Hit
TATGTAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA2090.12325802209208377No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC2080.12266827079020777No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTCTG2070.12207851948833176No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAGCAGTGG2030.11971951428082776No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.11795026037519979No Hit
TATGTAGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTCTG1990.11736050907332378No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1970.11618100646957179No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA1950.11500150386581978No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1870.11028349345081179No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.10910399084705981No Hit
TATGTAGGGGCTCCTTAAGCTCTCTTCCTTGTCCTGGCCAGAGGTCAATTTCCCTCCA1840.1085142395451838No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1810.1067449856395558No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.1043859804320518No Hit
TATGTAGGGGCCGGGCGTGGTGGCGCACACCTTTAATCCCAGCACTCGGGAGGCAGAG1730.1020269752245478No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1710.1008474726207958No Hit
TATGTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1700.10025772131891983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG150.007181633752.0510849
TATATCC150.007181633752.0510849
TGGCATA150.007181633752.0510852
ATATCCC150.007181633752.0510850
CTAGTAA203.4226407E-452.0510851
GACGAAC150.007181633752.0510852
ACGGTCA150.007181633752.0510851
AGCAATT203.4326344E-452.02036343
TCCGAAT203.4326344E-452.02036343
ATTCCTA203.4326344E-452.02036347
CGAATGC203.4326344E-452.02036345
TTCCTAG203.4326344E-452.02036348
ACTATCC150.007198423652.0203648
TTTGTCC150.007198423652.0203646
TAGGTGT150.007198423652.0203646
GACAAGG150.007198423652.0203646
CATAGAA150.007198423652.0203643
ACAGACG150.007198423652.0203647
CGAGACA150.007198423652.0203643
TCTCAAA353.8451617E-852.0203643
Produced by FastQC (version 0.11.5)