FastQCFastQC Report
Tue 31 May 2016
SRR212581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176626
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT31011.755687158176033No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13480.7631945466692333No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11510.6516594385877504No Hit
TATGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10190.5769252544925435No Hit
TATGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7910.4478389365099136No Hit
TATGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7730.4376479114060218No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6700.37933260108930733No Hit
TATGTAGGGATGGAAATAGTCTGTGATGTTCTTGTCAACATTCAGTATTTGATTACTT6490.36744307180143354No Hit
TATGTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC5010.28365019872498953No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4770.27006216525313376No Hit
TATGTAGGGGGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAGGGTCCCTCTT4610.2610034762718966No Hit
TATGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3840.21740853554969258No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.2157100313657106No Hit
TATGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA3790.21457769524305595No Hit
TATGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA3480.19702648534190892No Hit
TATGTAGGGTGAGCTTGGACAGGAGATGCCGCATCCTGCAGGGAGCCTGGAGAAGTGC3300.18683546023801706No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG3150.17834293931810719No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3140.17777677125677985No Hit
TATGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT3120.17664443513412523No Hit
TATGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3110.1760782670727979No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG2860.16192406553961478No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC2860.16192406553961478No Hit
TATGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2510.14210818339315842No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.12172613318537474No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA2140.12115996512404742No Hit
TATGTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2140.12115996512404742No Hit
TATGTAGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTCTG2130.12059379706272011No Hit
TATGTAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG2050.1160644525721015No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.11549828451077418No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.11436594838811953No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC2010.1137997803267922No Hit
TATGTAGGGGCTCCTTAAGCTCTCTTCCTTGTCCTGGCCAGAGGTCAATTTCCCTCCA1980.11210127614281022No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTCTG1970.11153510808148291No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1930.1092704358361736No Hit
TATGTAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1900.10757193165219164No Hit
TATGTAGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAGA1890.10700576359086432No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1870.10587342746820967No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1850.10474109134555501No Hit
TATGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1830.10360875522290036No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10191025103891839No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAGCAGTGG1790.10134408297759108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAA150.00715720452.0968227
GTTCGAA150.00715720452.0968227
TCATGCG150.00715720452.0968227
TGCGTTC150.00715720452.0968227
AATGTCC150.00716524652.08203525
ACGGTCG203.4129893E-452.0820324
TTGATAG150.00717329552.06725323
GTCATCC150.00717329552.06725351
CTGTCAA150.00717329552.06725350
GCAAGTT150.00717329552.06725322
GGCCGTT203.4177766E-452.06725322
CCTACGG203.4177766E-452.06725321
ATTCGGC150.00717329552.06725350
TATGCCT150.00717329552.06725322
CTTATGA251.6368685E-552.06725352
ACCAGCC150.00717329552.06725352
CTCATTC353.81915E-852.06725322
GCGATGC251.6368685E-552.06725321
CCAAGTT150.00717329552.06725323
ACATGCA150.00717329552.06725350
Produced by FastQC (version 0.11.5)