FastQCFastQC Report
Tue 31 May 2016
SRR212580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181314
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT29031.6010898220766185No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG12370.6822418566685419No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11170.6160583297483923No Hit
TATGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10520.5802089193333112No Hit
TATGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7350.40537410238591615No Hit
TATGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7050.3888282206558788No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6620.3651124568428252No Hit
TATGTAGGGATGGAAATAGTCTGTGATGTTCTTGTCAACATTCAGTATTTGATTACTT5800.31988704678072294No Hit
TATGTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC4760.2625279901165933No Hit
TATGTAGGGGGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAGGGTCCCTCTT4520.2492912847325634No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4340.23936375569454096No Hit
TATGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA3660.2018597571064562No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.1935868162414375No Hit
TATGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA3330.18365928720341507No Hit
TATGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3260.17979858146640634No Hit
TATGTAGGGTGAGCTTGGACAGGAGATGCCGCATCCTGCAGGGAGCCTGGAGAAGTGC3010.16601034669137518No Hit
TATGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2960.16325269973636894No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2960.16325269973636894No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG2950.1627011703453677No Hit
TATGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2920.16104658217236395No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAGCAGTGG2880.15884046460835896No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC2570.14174305348732033No Hit
TATGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2530.13953693592331534No Hit
TATGTAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA2340.12905787749429165No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG2060.11361505454625677No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.11306352515525553No Hit
TATGTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1960.10809976063624431No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.10809976063624431No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1930.10644517246324056No Hit
TATGTAGGGGCTCCTTAAGCTCTCTTCCTTGTCCTGGCCAGAGGTCAATTTCCCTCCA1910.10534211368123808No Hit
TATGTAGGGATTCTTGAGACCCTATGCTGTCACCAGAGGCTCTTTTAGGTGTAAGGTA1900.10479058429023684No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1880.10368752550823433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCG203.3341668E-452.3297168
GGATCCT203.3341668E-452.3297168
ATCCCCG150.007032308752.32971610
GATATTC150.007032308752.3297169
GATGCGT150.007032308752.3297169
GACGCAC150.007032308752.32971610
AGGGCGT550.052.3297166
GAGGGAC150.007032308752.3297169
GACTAAC150.007032308752.3297169
TAGAGCT150.00716481552.0833627
CGAACGG150.00716481552.0833627
ATGATAC150.00716481552.0833626
ATTAGCG353.810601E-852.0833629
GCGAACG150.00716481552.0833626
GACGTAT150.00716481552.0833628
TTGTCAC150.00716481552.0833628
ATAGAGC150.00716481552.0833626
GTAGCAT251.634038E-552.0833629
ACGTATG251.634038E-552.0833629
GCCAGTA251.634038E-552.0833625
Produced by FastQC (version 0.11.5)