FastQCFastQC Report
Tue 31 May 2016
SRR212579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201037
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT49132.443828747941921No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC15220.7570745683630377No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14530.7227525281415859No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC12520.6227709327138785No Hit
TATGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC10570.5257738625228192No Hit
TATGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC9420.46857046215373294No Hit
TATGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA7300.36311723712550426No Hit
TATGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6600.32829777603127785No Hit
TATGTAGGGATGGAAATAGTCTGTGATGTTCTTGTCAACATTCAGTATTTGATTAC6490.3228261464307565No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAGCAGT5400.26860727129831824No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.24672075289623305No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4830.24025428155016243No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4740.23577749369519044No Hit
TATGTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC4410.21936260489362652No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC4310.2143883961658799No Hit
TATGTAGGGGGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAGGGTCCCTC4240.21090645005645728No Hit
TATGTAGGGTGAGCTTGGACAGGAGATGCCGCATCCTGCAGGGAGCCTGGAGAAGT4090.20344513696483732No Hit
TATGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4080.20294771609206266No Hit
TATGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3870.1925018777637947No Hit
TATGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3310.16464630888841356No Hit
TATGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT3210.15967210016066694No Hit
TATGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3090.153703049687371No Hit
TATGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2880.14325721135910305No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2870.1427597904863284No Hit
TATGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2750.13679074001303243No Hit
TATGTAGGGATTGTTAACAAGCAAATGGTGTTTCGGTTAGTAACGGTTCTAAGTGC2710.13480105652193378No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.13380621477638444No Hit
TATGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2640.13131911041251113No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.12932942692141247No Hit
TATGTAGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTC2480.12336037644811652No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC2410.11987843033869387No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.11987843033869387No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.11788874684759522No Hit
TATGTAGGGGCTCCTTAAGCTCTCTTCCTTGTCCTGGCCAGAGGTCAATTTCCCTC2230.11092485462874993No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2170.10794032939210195No Hit
TATGTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT2090.10396096240990464No Hit
TATGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2020.10047901630048199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA204.0719303E-450.25520750
TTCAATA150.00825308750.25520750
ATATCCC150.00825308750.25520750
TGATTAC700.050.25520750
TTTTACG150.00825308750.25520750
CGGCCAC150.00826127950.242646
TTTCAAT150.00826127950.242649
TATTATA150.00826127950.242649
CGATGCA252.0224728E-550.242647
CGCAACT150.00826127950.242647
CGTCCGC150.00826127950.242648
TCGCCAA204.0820282E-450.2342
CATGCGG204.0820282E-450.2342
CTAAGGT150.00826947750.2343
CCCACTT150.00826947750.2343
TGTTGGG301.0109779E-650.2343
CACTATT301.0109779E-650.2345
TCTAAGG150.00826947750.2342
TAAGGTA150.00826947750.2344
CGATGGA150.00826947750.2344