FastQCFastQC Report
Tue 31 May 2016
SRR212578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182402
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT42892.3513996557055297No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC14940.8190699663380884No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13570.7439611407769652No Hit
TATGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC13120.719290358658348No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC12650.693523097334459No Hit
TATGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC9270.5082181116435127No Hit
TATGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6830.3744476486003443No Hit
TATGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6360.3486803872764553No Hit
TATGTAGGGATGGAAATAGTCTGTGATGTTCTTGTCAACATTCAGTATTTGATTAC5050.2768609993311477No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4890.2680891656889727No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.24287014396771966No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4330.2373877479413603No Hit
TATGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4330.2373877479413603No Hit
TATGTAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC4270.23409831032554468No Hit
TATGTAGGGGGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAGGGTCCCTC3830.2099757678095635No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAGCAGT3610.1979144965515729No Hit
TATGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3580.19626977774366508No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC3570.19572153814102913No Hit
TATGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT3130.17159899562504796No Hit
TATGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3120.17105075602241204No Hit
TATGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3110.1705025164197761No Hit
TATGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3020.16556835999605268No Hit
TATGTAGGGTGAGCTTGGACAGGAGATGCCGCATCCTGCAGGGAGCCTGGAGAAGT2970.162827161982873No Hit
TATGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2910.15953772436705738No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2560.14034933827479962No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.13870461946689183No Hit
TATGTAGGGATTGTTAACAAGCAAATGGTGTTTCGGTTAGTAACGGTTCTAAGTGC2400.13157750463262463No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.1304810254273528No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.12719158781153717No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.12390215019572155No Hit
TATGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2230.12225743138781374No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC2200.12061271257990593No Hit
TATGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2110.1156785561561825No Hit
TATGTAGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTC2020.11074439973245909No Hit
TATGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1970.10800320171927939No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1920.1052620037060997No Hit
TATGTAGGGGCTCCTTAAGCTCTCTTCCTTGTCCTGGCCAGAGGTCAATTTCCCTC1910.10471376410346378No Hit
TATGTAGGGACTTCTCTTATATTTTTACATCTGTGGAAGAAAAGCAATTCCTAGTA1840.10087608688501223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCA204.07965E-450.2334349
GTTGACA150.00826602350.23342550
CCCCTAT252.0239133E-550.23342550
ACCGACA150.00826602350.23342549
CACGAAC355.066977E-850.23342549
TCCTAGT150.00826602350.23342549
TGTTGAC150.00826602350.23342549
TGATTAC252.0239133E-550.23342550
TATTATA150.00826602350.23342549
TCCGTAA150.00826602350.23342550
ATTATAT150.00826602350.23342550
TAGTGGG150.00826602350.23342549
ACGAACT355.066977E-850.23342550
CGGGTTG150.00829314350.19183346
CGAAATG252.0338612E-550.19183346
TCATTAT204.0963673E-450.19183346
TGTGAAA150.00829314350.19183348
ACCTATA150.00829314350.19183348
TGACCGA150.00829314350.19183347
CAAGGCG204.0963673E-450.19183348
Produced by FastQC (version 0.11.5)