FastQCFastQC Report
Tue 31 May 2016
SRR212577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82810
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT14681.772732761743751No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT12991.5686511290906897No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10151.2256973795435333No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC9381.1327134404057482No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9341.1278831059050838No Hit
CCAGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8591.0373143340176307No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6050.7305880932254559No Hit
CCAGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3410.41178601618162053No Hit
CCAGACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2930.3538220021736505No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2440.29465040454051444No Hit
CCAGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2430.2934428209153484No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2190.26446081391136333No Hit
CCAGACGGGCTCCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2180.26325323028619735No Hit
CCAGACGGGATAGTTACTTTTCTTTCTGTTTGGCTTATTCCAGCCCCGAGTTTGATTA2150.25963047941069917No Hit
CCAGACGGGATCCACTCCTATTATCCGTAGGTTTGGTCTTCTCATTGTGTCCTGGATT2150.25963047941069917No Hit
CCAGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2010.24272430865837458No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.2378939741577104No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1920.2318560560318802No Hit
CCAGACGGGACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCC1870.22581813790605No Hit
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCAGAAAAACATCGAACT1710.20649679990339334No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGA1630.19683613090206498No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1560.18838304552590265No Hit
CCAGACGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG1550.1871754619007366No Hit
CCAGACGGGGGGAATTCTTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC1470.17751479289940827No Hit
CCAGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1460.17630720927424223No Hit
CCAGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1450.1750996256490762No Hit
CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1400.16906170752324598No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1330.16060862214708369No Hit
CCAGACGGGGTTGCTGGGCTTGCGTGAGCCACCCCGTGTAGTGTTGGGCTTGTATGAG1330.16060862214708369No Hit
CCAGACGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1280.15457070402125347No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTA1170.141287284144427No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1130.13645694964376284No Hit
CCAGACGGGGAGTGCCCCAGACTTTAACTGTGTAAATAAGAGTGTTTCATTCAAATGT1120.1352493660185968No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.1352493660185968No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1120.1352493660185968No Hit
CCAGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1110.13404178239343076No Hit
CCAGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1110.13404178239343076No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1080.13041903151793263No Hit
CCAGACGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1070.1292114478927666No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1020.12317352976693637No Hit
CCAGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG1010.12196594614177032No Hit
CCAGACGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTG1010.12196594614177032No Hit
CCAGACGGGAGTAATTGAGAGTTTTGCTTGGTATAGTAGCCTGGGCTGGCATTTTTGT980.1183431952662722No Hit
CCAGACGGGAAGATTGCCGTGGCCCTGGCTGTTGCCGCAATCCCTGAGGGTCTGCCTG930.11230527714044197No Hit
CCAGACGGGATTAAAGGCATGCACTACAATATATGACTTTCAACTTGACTTATTTCCA920.11109769351527593No Hit
CCAGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA900.10868252626494385No Hit
CCAGACGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG890.1074749426397778No Hit
CCAGACGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT890.1074749426397778No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT870.10505977538944572No Hit
CCAGACGGGCAGTTTCTCAATCATCTCAAATACCCACAGGCAAGTTAACTGGTGAGTT860.10385219176427968No Hit
CCAGACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG850.10264460813911364No Hit
CCAGACGGGAAGAGAATTTCTGTAGAATGAAATAGAGCCTTCAGAAACTTGACTCAGA850.10264460813911364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATG203.4115074E-452.0538152
ATCCAAG150.00716722152.0538151
TTCAAAT203.4115074E-452.0538150
CATCCAA150.00716722152.0538150
CCGTCAT203.4115074E-452.0538151
TCAAATG150.00716722152.0538151
CCCGTCA203.4115074E-452.0538150
TGGGGCG150.00716722152.0538152
TACCGTA150.00716722152.0538152
TCCAAGC150.00716722152.0538152
CAAATGT150.00716722152.0538152
ACGCGGT150.00716722152.0538152
CCCTATT150.00716722152.0538151
CTACCGT150.00716722152.0538151
CCTGGAT203.4115074E-452.0538151
GGTTCGC600.052.02235845
CTGACCA150.007184385352.02235843
ACATCCA150.007184385352.02235849
GACATCC150.007184385352.02235848
TCTCGTG150.007184385352.02235843
Produced by FastQC (version 0.11.5)