##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212577_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82810 Sequences flagged as poor quality 0 Sequence length 58 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.201485327858954 38.0 36.0 39.0 33.0 40.0 2 36.66754015215554 38.0 36.0 39.0 32.0 40.0 3 35.831445477599324 38.0 35.0 39.0 30.0 40.0 4 35.367274483758 38.0 33.0 39.0 29.0 40.0 5 35.38294891921266 38.0 34.0 39.0 29.0 40.0 6 36.30108682526265 38.0 35.0 39.0 31.0 40.0 7 36.278637845670815 38.0 35.0 39.0 31.0 40.0 8 36.6104939017027 38.0 36.0 39.0 32.0 40.0 9 36.563494747011234 38.0 36.0 39.0 32.0 40.0 10 35.0854365414805 38.0 33.0 39.0 28.0 40.0 11 35.846950851346456 38.0 35.0 39.0 30.0 40.0 12 35.337048665620095 38.0 34.0 39.0 29.0 40.0 13 35.24628668035261 38.0 34.0 39.0 28.0 40.0 14 35.29582176065693 38.0 34.0 39.0 29.0 40.0 15 35.20710059171598 38.0 33.0 39.0 29.0 40.0 16 34.68037676609105 38.0 33.0 39.0 27.0 40.0 17 34.56466610312764 37.0 33.0 39.0 27.0 40.0 18 34.46452119309262 37.0 33.0 39.0 27.0 40.0 19 34.177623475425676 36.0 33.0 39.0 26.0 40.0 20 34.247144064726484 37.0 33.0 39.0 27.0 40.0 21 34.06712957372298 37.0 33.0 38.0 26.0 40.0 22 33.759364811013164 36.0 32.0 38.0 25.0 40.0 23 33.409068953025 36.0 31.0 38.0 24.0 39.0 24 33.20066417099384 36.0 31.0 38.0 23.0 39.0 25 33.252735176911 36.0 31.0 38.0 24.0 39.0 26 32.69176427967637 36.0 31.0 38.0 22.0 39.0 27 32.44065934065934 35.0 30.0 38.0 21.0 39.0 28 32.13697621060258 35.0 30.0 38.0 20.0 39.0 29 32.231457553435575 35.0 30.0 38.0 21.0 39.0 30 32.05913537012438 35.0 30.0 38.0 20.0 39.0 31 31.427738195870063 35.0 29.0 38.0 17.0 39.0 32 31.147470112305278 35.0 29.0 38.0 16.0 39.0 33 30.922654268808113 35.0 29.0 38.0 15.0 39.0 34 30.494722859558024 35.0 28.0 38.0 13.0 39.0 35 30.133607052288372 35.0 27.0 38.0 11.0 39.0 36 31.15634585195025 35.0 30.0 38.0 12.0 39.0 37 30.92632532302862 35.0 29.0 38.0 12.0 39.0 38 30.952203840115928 35.0 29.0 38.0 11.0 39.0 39 30.874387151310227 35.0 29.0 38.0 10.0 39.0 40 30.603755585074268 35.0 29.0 38.0 9.0 39.0 41 30.991812583021375 35.0 30.0 38.0 2.0 39.0 42 30.7545344765125 35.0 29.0 38.0 2.0 39.0 43 30.426434005554885 35.0 29.0 38.0 2.0 39.0 44 30.419224731312642 35.0 29.0 38.0 2.0 39.0 45 30.038063035865235 35.0 28.0 38.0 2.0 39.0 46 29.576427967636757 35.0 27.0 38.0 2.0 39.0 47 29.4960028982007 35.0 27.0 38.0 2.0 39.0 48 29.670498732037192 35.0 28.0 38.0 2.0 39.0 49 29.31272793140925 35.0 27.0 38.0 2.0 39.0 50 29.10896027049873 35.0 27.0 38.0 2.0 39.0 51 28.764497041420118 35.0 26.0 38.0 2.0 39.0 52 28.510059171597632 35.0 26.0 38.0 2.0 39.0 53 28.238340780099023 35.0 25.0 38.0 2.0 39.0 54 28.05361671295737 35.0 25.0 38.0 2.0 39.0 55 27.603658978384253 35.0 23.0 38.0 2.0 39.0 56 27.23935515034416 35.0 20.0 38.0 2.0 39.0 57 26.704335225214347 34.0 17.0 38.0 2.0 39.0 58 26.380823572032362 34.0 16.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 -1.823242478243614 1 2 -3.6064951459975454 1 3 -4.546340513267904 1 4 -3.9507901919243977 1 5 -3.107819050636106 1 6 -5.263628640539732 1 7 -4.017448385755976 1 8 -4.306895594099053 1 9 -4.326308161142258 1 10 -2.9247301140951016 1 11 0.1385484186720234 1 12 -2.4038738141526146 1 13 -3.868078877497684 1 14 -6.0528552079084825 1 15 -5.268759225613433 1 16 -4.055357659936835 1 17 -5.8734832814604765 1 18 -3.9433943842266466 1 19 -5.592653476533236 1 20 -5.306473068016448 1 21 -7.011055523337749 1 22 -7.5905237487870885 1 23 -6.979489256402182 1 24 -5.922288212326137 1 25 -6.090927944911119 1 26 -5.163242287968718 1 27 -5.4786397117945675 1 28 -3.145614946167548 1 29 -3.874175207667456 1 30 -4.660925499868689 1 31 -5.312476298159403 1 32 -4.317701765568689 1 33 -4.674875905168914 1 34 -3.380803841589504 1 35 -2.483609395078492 1 36 -2.7687891246547878 1 37 -2.766973941103487 1 38 -1.5222818280790307 1 39 -3.0953952408368153 1 40 -4.599306798589083 1 41 -2.4987957499568125 1 42 -2.524017006333114 1 43 -3.5245886012992607 1 44 -3.2046911162717926 1 45 -3.0014397489695277 1 46 -3.4852078122821624 1 47 -3.0366486792349257 1 48 -3.0377317803748554 1 49 -4.007424050209682 1 50 -1.758087931056071 1 51 -6.492068787577452 1 52 -3.167421728332112 1 53 -3.003679648162816 1 54 -2.467540314317592 1 55 -3.5299499917494934 1 56 -1.0138530246338675 1 57 -0.703965962986004 1 58 -2.325377131560135 2 1 -4.010742478243614 2 2 -6.368995145997545 2 3 -4.283840513267904 2 4 -4.788290191924396 2 5 -5.970319050636107 2 6 -5.026128640539731 2 7 -5.417448385755975 2 8 -4.781895594099055 2 9 -5.263808161142258 2 10 -5.874730114095101 2 11 -4.423951581327977 2 12 -6.016373814152615 2 13 -3.8805788774976833 2 14 -4.190355207908482 2 15 -4.593759225613432 2 16 -4.4178576599368355 2 17 -5.048483281460477 2 18 -3.830894384226646 2 19 -4.055153476533235 2 20 -4.568973068016447 2 21 -6.711055523337748 2 22 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53 -3.037012981496151 118 54 -2.967540314317592 118 55 -3.3632833250828256 118 56 -2.3471863579671997 118 57 -4.370632629652672 118 58 -1.625377131560132 119 1 -2.135742478243614 119 2 -4.243995145997545 119 3 -4.783840513267904 119 4 -4.600790191924396 119 5 -4.532819050636107 119 6 -4.588628640539731 119 7 -4.417448385755975 119 8 -2.969395594099055 119 9 -2.763808161142258 119 10 -5.062230114095101 119 11 -2.1114515813279766 119 12 -1.1413738141526153 119 13 -3.1930788774976833 119 14 -2.252855207908482 119 15 -2.2187592256134323 119 16 -6.2928576599368355 119 17 -5.673483281460477 119 18 -5.580894384226646 119 19 -5.117653476533235 119 20 -6.756473068016447 119 21 -5.086055523337748 119 22 -7.015523748787089 119 23 -5.454489256402184 119 24 -5.509788212326136 119 25 -6.20342794491112 119 26 -6.388242287968719 119 27 -4.9161397117945675 119 28 -4.558114946167549 119 29 -4.086675207667454 119 30 -3.6109254998686886 119 31 -5.862476298159404 119 32 -4.692701765568689 119 33 -3.799875905168914 119 34 -4.055803841589505 119 35 -5.033609395078493 119 36 -4.3187891246547885 119 37 -3.9919739411034882 119 38 -3.6972818280790314 119 39 -2.3578952408368146 119 40 -2.899306798589084 119 41 -2.5487957499568132 119 42 -0.3490170063331135 119 43 -1.03708860129926 119 44 -1.4546911162717926 119 45 -1.576439748969527 119 46 -1.7352078122821624 119 47 -0.5866486792349264 119 48 0.23726821962514322 119 49 -0.6699240502096835 119 50 -0.2705879310560704 119 51 1.0954312124225467 119 52 1.5325782716678873 119 53 0.20882035183718273 119 54 -1.155040314317592 119 55 0.195050008250508 119 56 -2.3888530246338675 119 57 -1.2539659629860047 119 58 -0.4753771315601334 120 1 -1.4232424782436155 120 2 -2.2064951459975433 120 3 -6.146340513267905 120 4 -2.9507901919243977 120 5 -1.4078190506361068 120 6 -0.7636286405397286 120 7 -1.5174483857559764 120 8 -1.3068955940990534 120 9 -1.3263081611422578 120 10 -0.9247301140950981 120 11 -1.2614515813279752 120 12 -2.9038738141526146 120 13 -2.1680788774976847 120 14 -3.8528552079084832 120 15 -1.1687592256134351 120 16 -2.755357659936834 120 17 -0.1734832814604772 120 18 -2.0433943842266444 120 19 1.0073465234667651 120 20 0.19352693198355553 120 21 -0.011055523337745399 120 22 -1.1905237487870899 120 23 1.0205107435978178 120 24 0.7777117876738657 120 25 1.1090720550888804 120 26 3.4367577120312838 120 27 0.7213602882054317 120 28 3.354385053832452 120 29 1.7258247923325456 120 30 0.6390745001313114 120 31 2.6875237018405933 120 32 2.882298234431314 120 33 4.225124094831084 120 34 4.919196158410497 120 35 1.4163906049215065 120 36 4.031210875345213 120 37 4.433026058896509 120 38 3.6777181719209686 120 39 3.304604759163187 120 40 5.300693201410919 120 41 3.1012042500431853 120 42 2.5759829936668837 120 43 1.77541139870074 120 44 4.295308883728207 120 45 2.298560251030473 120 46 1.8147921877178383 120 47 3.8633513207650765 120 48 1.7622682196251418 120 49 0.19257594979031722 120 50 3.941912068943928 120 51 4.507931212422548 120 52 4.8325782716678845 120 53 4.296320351837181 120 54 2.7324596856824073 120 55 2.4700500082505066 120 56 1.9861469753661325 120 57 1.8960340370139939 120 58 2.7746228684398666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 673.0 3 12.0 4 39.0 5 57.0 6 118.0 7 203.0 8 157.0 9 204.0 10 276.0 11 305.0 12 375.0 13 397.0 14 461.0 15 561.0 16 642.0 17 639.0 18 708.0 19 748.0 20 701.0 21 756.0 22 893.0 23 1007.0 24 1181.0 25 1356.0 26 1583.0 27 1971.0 28 2383.0 29 2867.0 30 3391.0 31 3841.0 32 4614.0 33 5288.0 34 6025.0 35 6973.0 36 7877.0 37 10149.0 38 8966.0 39 4407.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 43.2254558628185 36.237169423982614 5.2324598478444635 15.304914865354426 11 27.647627098176546 26.409853882381356 19.3563579277865 26.586161091655597 12 34.053858229682405 15.821760656925493 28.83226663446444 21.292114478927665 13 18.032846274604516 18.474821881415288 32.44897959183673 31.043352252143464 14 16.427967636758844 26.342229199372056 34.77720082115687 22.452602342712233 15 25.31578311798092 19.137785291631445 35.25419635309745 20.292235237290182 16 23.580485448617317 18.903514068349235 30.66175582659099 26.85424465644246 17 27.100918982236234 19.951937591324615 18.367568742527986 34.57957468391117 18 27.40128003864268 16.49076198526748 25.378577466489556 30.72938050960029 19 26.895627286889106 18.145370672269923 30.81549106981125 24.14351097102972 20 28.44497578765593 22.539820550906303 24.566170343803208 24.449033317634555 21 32.35876627297539 16.37683259667174 28.15375697413231 23.110644156220562 22 35.76258905929235 17.6741939379302 20.190798212776233 26.372418790001205 23 31.821036106750395 26.031880207704383 21.52276295133438 20.624320734210844 24 27.523245984784445 29.605120154570702 21.54087670571187 21.33075715493298 25 27.53444673888104 25.584175633083362 20.76706638167349 26.11431124636211 26 29.969924993658886 20.292778374983392 21.388281618011185 28.34901501334654 27 22.597669222873016 22.595253909788056 23.666445262967212 31.140631604371716 28 25.989539925837352 25.828894445048373 26.632121848993247 21.549443780121027 29 28.31467188462514 17.384739887185805 30.23033904651472 24.070249181674335 30 21.09002162645435 23.883337964696928 29.20417064359845 25.822469765250272 31 27.930888660665744 22.073340180027788 28.754908475804992 21.24086268350148 32 30.304494341309045 18.257582162743226 32.015991690118724 19.421931805828997 33 31.02536231884058 16.154589371980677 31.481884057971016 21.33816425120773 34 29.533622428642175 18.59833065577929 33.63813159070869 18.229915324869847 35 26.57212572174039 25.962119198898364 33.145700963931105 14.320054115430144 36 26.06715236748946 25.39659526139645 31.386905409160654 17.149346961953434 37 23.11698040258077 23.333252784960006 35.895174346954065 17.65459246550516 38 23.916957693376677 22.940558046233974 33.139584063417644 20.00290019697171 39 24.260512324794586 23.657564040599325 33.485983566940554 18.595940067665538 40 25.143796220213638 22.692010246991153 35.32674368021654 16.837449852578665 41 25.875756733569368 19.969307732276423 36.395712749374674 17.759222784779535 42 23.854419745923536 20.652476097230785 34.346254729182526 21.14684942766315 43 24.194406169168662 19.64609471317717 36.76118645296976 19.39831266468441 44 25.12175962293794 17.40165568916551 36.415493383286005 21.06109130461055 45 24.020786754486675 22.01099764336214 35.49942594718714 18.468789654964045 46 23.28990621676496 23.013148989654837 36.96098810789906 16.735956685681135 47 23.484253158435592 22.572689355014205 36.208668318926435 17.734389167623767 48 26.14430447082719 22.530647774258288 33.490098411393475 17.834949343521046 49 21.026093685763342 23.749123358564482 33.4139440400474 21.81083891562477 50 17.792019347037485 20.203143893591292 36.16082224909311 25.844014510278114 51 18.194457877937907 21.76951349260083 38.59174001354096 21.4442886159203 52 18.536426162530674 20.816158783497926 43.09734192363017 17.550073130341236 53 16.37726723095526 23.252720677146314 38.67351874244257 21.696493349455864 54 20.87303506650544 21.2043530834341 37.0459492140266 20.876662636033856 55 20.085387387823225 23.27834353305435 35.41641469727389 21.219854381848528 56 18.300693125597263 24.881152548113562 32.928909264657854 23.889245061631325 57 17.867985920433515 21.818489712481707 38.23497393344864 22.07855043363613 58 19.6537875305446 22.859458544020516 36.87005540367261 20.616698521762274 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 29.0 12 58.0 13 82.0 14 106.0 15 127.0 16 162.0 17 176.0 18 329.0 19 482.0 20 614.0 21 746.0 22 740.0 23 813.0 24 892.0 25 1215.5 26 1539.0 27 1758.5 28 2269.0 29 2560.0 30 2555.0 31 2550.0 32 2387.0 33 2224.0 34 2118.0 35 2344.5 36 2677.0 37 2176.0 38 1675.0 39 1490.5 40 1306.0 41 1228.0 42 1322.0 43 1494.0 44 1401.5 45 1309.0 46 2407.0 47 3419.0 48 3333.0 49 2575.5 50 1818.0 51 1864.0 52 1910.0 53 2222.5 54 3339.5 55 4144.0 56 3789.5 57 3435.0 58 3249.0 59 3494.0 60 3925.0 61 4327.0 62 4729.0 63 4680.5 64 4632.0 65 5402.0 66 5303.0 67 4434.0 68 3854.0 69 3274.0 70 2728.5 71 2183.0 72 1740.5 73 1135.0 74 972.0 75 830.5 76 689.0 77 548.5 78 322.5 79 237.0 80 200.0 81 163.0 82 112.5 83 62.0 84 48.0 85 27.5 86 21.0 87 15.5 88 10.0 89 7.5 90 5.0 91 3.5 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0012075836251660428 18 0.0 19 0.0012075836251660428 20 0.0012075836251660428 21 0.004830334500664171 22 0.0 23 0.0 24 0.0 25 0.0012075836251660428 26 0.020528921627822726 27 0.006037918125830213 28 0.02294408887815481 29 0.02294408887815481 30 0.049510928631807755 31 0.05434126313247192 32 0.020528921627822726 33 0.012075836251660426 34 0.027774423378818983 35 0.028982007003985025 36 0.05192609588213984 37 0.05313367950730588 38 0.06883226663446444 39 0.06037918125830214 40 0.06520951575896632 41 0.06400193213380027 42 0.09539910638811737 43 0.09177635551261924 44 0.07849293563579278 45 0.07849293563579278 46 0.07970051926095882 47 0.11472044439077406 48 0.1159280280159401 49 0.13041903151793263 50 0.13283419876826472 51 0.1183431952662722 52 0.09781427363844947 53 0.13283419876826472 54 0.13283419876826472 55 0.15457070402125347 56 0.17026929114841202 57 0.16543895664774788 58 0.17389204202391015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 82810.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.28124622630117 #Duplication Level Percentage of deduplicated Percentage of total 1 73.47959631417288 30.333293080545825 2 11.4289893228024 9.43605844704746 3 4.943688752376774 6.122448979591836 4 2.5859295012432355 4.270015698587127 5 1.8780166739798159 3.8763434367829976 6 1.1262249524645311 2.789518174133559 7 0.9536346350738628 2.7557058326289097 8 0.6201550387596899 2.0480618282816088 9 0.45341524060260346 1.6845791571066298 >10 2.2583004241626443 17.019683613090205 >50 0.1491882404563405 4.1987682647023306 >100 0.10238408658768466 6.869943243569618 >500 0.01170103846716396 4.028498973553918 >1k 0.008775778850372971 4.567081270377973 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 1468 1.772732761743751 No Hit CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 1299 1.5686511290906897 No Hit CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1015 1.2256973795435333 No Hit CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 938 1.1327134404057482 No Hit CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 934 1.1278831059050838 No Hit CCAGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 859 1.0373143340176307 No Hit CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 605 0.7305880932254559 No Hit CCAGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 341 0.41178601618162053 No Hit CCAGACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 293 0.3538220021736505 No Hit CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 244 0.29465040454051444 No Hit CCAGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 243 0.2934428209153484 No Hit CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 219 0.26446081391136333 No Hit CCAGACGGGCTCCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG 218 0.26325323028619735 No Hit CCAGACGGGATAGTTACTTTTCTTTCTGTTTGGCTTATTCCAGCCCCGAGTTTGATTA 215 0.25963047941069917 No Hit CCAGACGGGATCCACTCCTATTATCCGTAGGTTTGGTCTTCTCATTGTGTCCTGGATT 215 0.25963047941069917 No Hit CCAGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 201 0.24272430865837458 No Hit CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.2378939741577104 No Hit CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT 192 0.2318560560318802 No Hit CCAGACGGGACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCC 187 0.22581813790605 No Hit CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCAGAAAAACATCGAACT 171 0.20649679990339334 No Hit CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGA 163 0.19683613090206498 No Hit CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC 156 0.18838304552590265 No Hit CCAGACGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG 155 0.1871754619007366 No Hit CCAGACGGGGGGAATTCTTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC 147 0.17751479289940827 No Hit CCAGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 146 0.17630720927424223 No Hit CCAGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 145 0.1750996256490762 No Hit CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 140 0.16906170752324598 No Hit CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC 133 0.16060862214708369 No Hit CCAGACGGGGTTGCTGGGCTTGCGTGAGCCACCCCGTGTAGTGTTGGGCTTGTATGAG 133 0.16060862214708369 No Hit CCAGACGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA 128 0.15457070402125347 No Hit CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTA 117 0.141287284144427 No Hit CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC 113 0.13645694964376284 No Hit CCAGACGGGGAGTGCCCCAGACTTTAACTGTGTAAATAAGAGTGTTTCATTCAAATGT 112 0.1352493660185968 No Hit CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.1352493660185968 No Hit CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC 112 0.1352493660185968 No Hit CCAGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 111 0.13404178239343076 No Hit CCAGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 111 0.13404178239343076 No Hit CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC 108 0.13041903151793263 No Hit CCAGACGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA 107 0.1292114478927666 No Hit CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 102 0.12317352976693637 No Hit CCAGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG 101 0.12196594614177032 No Hit CCAGACGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTG 101 0.12196594614177032 No Hit CCAGACGGGAGTAATTGAGAGTTTTGCTTGGTATAGTAGCCTGGGCTGGCATTTTTGT 98 0.1183431952662722 No Hit CCAGACGGGAAGATTGCCGTGGCCCTGGCTGTTGCCGCAATCCCTGAGGGTCTGCCTG 93 0.11230527714044197 No Hit CCAGACGGGATTAAAGGCATGCACTACAATATATGACTTTCAACTTGACTTATTTCCA 92 0.11109769351527593 No Hit CCAGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 90 0.10868252626494385 No Hit CCAGACGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG 89 0.1074749426397778 No Hit CCAGACGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 89 0.1074749426397778 No Hit CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 87 0.10505977538944572 No Hit CCAGACGGGCAGTTTCTCAATCATCTCAAATACCCACAGGCAAGTTAACTGGTGAGTT 86 0.10385219176427968 No Hit CCAGACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 85 0.10264460813911364 No Hit CCAGACGGGAAGAGAATTTCTGTAGAATGAAATAGAGCCTTCAGAAACTTGACTCAGA 85 0.10264460813911364 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.003622750875498128 0.0 0.0 0.0 0.0 11 0.012075836251660428 0.0 0.0 0.0 0.0 12 0.012075836251660428 0.0 0.0 0.0 0.0 13 0.012075836251660428 0.0 0.0 0.0 0.0 14 0.019321338002656684 0.0 0.0 0.0 0.0 15 0.02294408887815481 0.0 0.0 0.0 0.0 16 0.02294408887815481 0.0 0.0 0.0 0.0 17 0.024151672503320856 0.0 0.0 0.0 0.0 18 0.02656683975365294 0.0 0.0 0.0 0.0 19 0.02656683975365294 0.0 0.0 0.0 0.0 20 0.04105784325564545 0.0 0.0 0.0 0.0 21 0.04105784325564545 0.0 0.0 0.0 0.0 22 0.042265426880811495 0.0 0.0 0.0 0.0 23 0.055548846757637965 0.0 0.0 0.0 0.0 24 0.0652095157589663 0.0 0.0 0.0 0.0 25 0.06641709938413234 0.0 0.0 0.0 0.0 26 0.06883226663446444 0.0 0.0 0.0 0.0 27 0.07003985025963048 0.0 0.0 0.0 0.0 28 0.10385219176427968 0.0 0.0 0.0 0.0 29 0.10989010989010989 0.0 0.0 0.0 0.0 30 0.13162661514309865 0.0 0.0 0.0 0.0 31 0.13404178239343076 0.0 0.0 0.0 0.0 32 0.13645694964376284 0.0 0.0 0.0 0.0 33 0.13887211689409493 0.0 0.0 0.0 0.0 34 0.14007970051926097 0.0 0.0 0.0 0.0 35 0.23306363965704624 0.0 0.0 0.0 0.0 36 0.23427122328221228 0.0 0.0 0.0 0.0 37 0.23668639053254437 0.0 0.0 0.0 0.0 38 0.23910155778287645 0.0 0.0 0.0 0.0 39 0.2451394759087067 0.0 0.0 0.0 0.0 40 0.24876222678420482 0.0 0.0 0.0 0.0 41 0.2523849776597029 0.0 0.0 0.0 0.0 42 0.2608380630358652 0.0 0.0 0.0 0.0 43 0.2632532302861973 0.0 0.0 0.0 0.0 44 0.26446081391136333 0.0 0.0 0.0 0.0 45 0.2668759811616955 0.0 0.0 0.0 0.0 46 0.26929114841202756 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCATG 20 3.4115074E-4 52.05381 52 ATCCAAG 15 0.007167221 52.05381 51 TTCAAAT 20 3.4115074E-4 52.05381 50 CATCCAA 15 0.007167221 52.05381 50 CCGTCAT 20 3.4115074E-4 52.05381 51 TCAAATG 15 0.007167221 52.05381 51 CCCGTCA 20 3.4115074E-4 52.05381 50 TGGGGCG 15 0.007167221 52.05381 52 TACCGTA 15 0.007167221 52.05381 52 TCCAAGC 15 0.007167221 52.05381 52 CAAATGT 15 0.007167221 52.05381 52 ACGCGGT 15 0.007167221 52.05381 52 CCCTATT 15 0.007167221 52.05381 51 CTACCGT 15 0.007167221 52.05381 51 CCTGGAT 20 3.4115074E-4 52.05381 51 GGTTCGC 60 0.0 52.022358 45 CTGACCA 15 0.0071843853 52.022358 43 ACATCCA 15 0.0071843853 52.022358 49 GACATCC 15 0.0071843853 52.022358 48 TCTCGTG 15 0.0071843853 52.022358 43 >>END_MODULE