FastQCFastQC Report
Tue 31 May 2016
SRR212576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86669
Sequences flagged as poor quality0
Sequence length58
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT15071.7387993400177688No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT13581.5668808916683012No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC9961.1491998292353667No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9881.1399693085186169No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9351.0788171087701484No Hit
CCAGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8510.9818966412442741No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6190.7142115404585261No Hit
CCAGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4210.4857561527189653No Hit
CCAGACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2950.3403754514301538No Hit
CCAGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2510.2896075874880292No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2370.27345417623371676No Hit
CCAGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2210.2549931348002169No Hit
CCAGACGGGCTCCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2200.2538393197106232No Hit
CCAGACGGGATAGTTACTTTTCTTTCTGTTTGGCTTATTCCAGCCCCGAGTTTGATTA2140.24691642917306073No Hit
CCAGACGGGATCCACTCCTATTATCCGTAGGTTTGGTCTTCTCATTGTGTCCTGGATT2140.24691642917306073No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.24114735372509202No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1920.22153249720199839No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1860.21460960666443593No Hit
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCAGAAAAACATCGAACT1860.21460960666443593No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1810.20884053121646723No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGA1730.1996100104997173No Hit
CCAGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1680.1938409350517486No Hit
CCAGACGGGACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCC1650.19037948978296737No Hit
CCAGACGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG1620.18691804451418614No Hit
CCAGACGGGGGGAATTCTTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC1480.1707646332598738No Hit
CCAGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1370.1580726672743426No Hit
CCAGACGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1330.15345740691596763No Hit
CCAGACGGGGTTGCTGGGCTTGCGTGAGCCACCCCGTGTAGTGTTGGGCTTGTATGAG1320.1523035918263739No Hit
CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1320.1523035918263739No Hit
CCAGACGGGGAGTGCCCCAGACTTTAACTGTGTAAATAAGAGTGTTTCATTCAAATGT1310.15114977673678015No Hit
CCAGACGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1290.14884214655759267No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.14307307110962397No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1230.14191925602003022No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1220.1407654409304365No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1190.13730399566165527No Hit
CCAGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1170.13499636548246777No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1120.12922729003449906No Hit
CCAGACGGGAAGATTGCCGTGGCCCTGGCTGTTGCCGCAATCCCTGAGGGTCTGCCTG1070.12345821458653036No Hit
CCAGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG1060.12230439949693662No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTA1010.11653532404896792No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1010.11653532404896792No Hit
CCAGACGGGAAGGACTGCACGGTGAAGATCTGGAACAACGACCTGACCATCTCACTGC990.11422769386978043No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC990.11422769386978043No Hit
CCAGACGGGAGTAATTGAGAGTTTTGCTTGGTATAGTAGCCTGGGCTGGCATTTTTGT990.11422769386978043No Hit
CCAGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT990.11422769386978043No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT980.11307387878018668No Hit
CCAGACGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTG930.10730480333221797No Hit
CCAGACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC930.10730480333221797No Hit
CCAGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA920.10615098824262423No Hit
CCAGACGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT900.10384335806343675No Hit
CCAGACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG890.102689542973843No Hit
CCAGACGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG890.102689542973843No Hit
CCAGACGGGATGTCTTTAAGAAATGTTTGCAAACTGTCATTTCCAACCTTACAGATTG890.102689542973843No Hit
CCAGACGGGAAGAGAATTTCTGTAGAATGAAATAGAGCCTTCAGAAACTTGACTCAGA880.10153572788424926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCA203.4003728E-452.0909125
CTCAATG307.8138874E-752.0909126
TGCTTAC150.00714812252.0909152
TTGTCCA150.00714812252.0909126
CCTAACG150.00714812252.0909151
GTGTGTA150.00714812252.0909126
AGTATGT150.00714812252.0909124
TCAATGG307.8138874E-752.0909127
GTTGTCC150.00714812252.0909125
GAGGTTA150.00714812252.0909152
CTACCGT150.00714812252.0909151
GACATTC203.4003728E-452.0909152
CGTGTGA150.00714812252.0909152
GAGTTAC150.00714812252.0909152
TTTGCAA150.00714812252.0909126
CGGTCCC203.4003728E-452.0909124
AAGATCT150.00714812252.0909125
TTGCAAA150.00714812252.0909127
GCATGAG203.4003728E-452.0909126
TATGTGT150.00714812252.0909126
Produced by FastQC (version 0.11.5)